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Conservative Dentistry Journal

CDJ
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Published by Universitas Airlangga

ISSN : 20871848 EISSN : 27228045 DOI : 10.20473/cdj.v10i1.2020.1-4

Core Subject : Health,

Dentistry

Journal of conservative dentistry accepts original manuscripts in the field of Endodontic other related subjects articles, including research, case reports and literature reviews. The spread of fields include: Endodontic research; Preventive, curative and rehabilitative related to endodontic field; Oral health education and promotion related to endodontic field; Endodontic and restorative clinical research; Basic sciences related to endodontic field; Endodontic healthcare management

Arjuna Subject : Kedokteran Gigi - Kedokteran Gigi (Lain-Lain)

Articles 190 Documents

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Daya Hambat Aktivitas Enzim Glukosiltransferase (Gtf) Streptococcus mutans Oleh Ekstrak Temulawak (Curcuma Xanthorrhiza Roxb.) A. Amanda; Sri Kunarti; Agus Subiwahjudi
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background: Streptococcus mutans is a bacteria which has glucosyltransferase (GTF) enzyme and acts as the main agent that causes dental caries. GTF enzyme will convert sucrose into fructose and glucan. Temulawak (Curcuma xanthorrhiza Roxb.) is one of the traditional herbs which has xanthorrhizol, curcumin, flavonoid, tanin, and saponin as an antibacterial agent. Purpose: The purpose of this research is to investigate the effect of temulawak extract (Curcuma xanthorrhiza Roxb.) to the activity of GTF enzyme Streptococcus mutans. Method: This research used 25%, 37,5%, and 50% concentration of temulawak extract as the treatment, and 0.12% chlorhexidine gluconate as a control. The method of this research consists of three steps; preparing the temulawak extract concentration of 25%, 37,5%, and 50%, preparing the GTF enzyme from the supernatant of Streptococcus mutans, and testing GTF enzyme activity by analyzing the fructose concentration using High Performance Liquid Chromatography (HPLC). Perusal of the fructose area was based on the retention time of fructose. One unit of GTF enzyme activity is defined as the 1 μmol fructose / ml of enzyme / hour. Result: The obtained data then were analyzed by Post-Hoc Tukey (HSD). The result showed a significant difference between each treatment group with the control group (p<0.05). Conclussion: This research concludes that temulawak extract with 25%, 37,5%, and 50% concentrationcan’t inhibit the GTF enzyme activity of Streptococcus mutans.

The Difference of The Effects of Conventional Flowable Composite and Self-Adhering Flowable Composite on BHK-21 Fibroblast Cells Anuj Bhardwaj; S. Sukaton; Anggi Puspitasari; Widya Saraswati
Conservative Dentistry Journal Vol. 8 No. 2 (2018): July - December
Publisher : Universitas Airlangga

Background: One type of composite resin material on the market is flowable composites (FC) which has low viscosity, can be applied to areas with low stress or require good penetration such as pit and fissure sealants, restoration of class II, class III, and class V. Along with technology development, self-adhering flowable composite (SAFC) material has been developed which shorten the applications time because it combines etch, priming, and bonding in one system. The incomplete composite polymerization process can release residual monomers which affect pulp and gingiva The effects of composite materials can be seen from the viability of BHK-21 fibroblast cells after being exposed by these materials. Aims: Determine the viability of BHK-21 cells after being exposed to conventional flowable composite (CFC) and SAFC. Method: The research was in-vitro experimental laboratory with post-test only control group design. BHK-21 cell cultures were included in a 96-well microplate and divided into control group (N=16) and two treated groups (N=16). The treated group was given CFC and SAFC in a disk form with 5mm in diameter and 2,5mm in thickness, then incubated for 24 hours. MTT was given, the optical density value was read by ELISA reader and cell viability was calculated. Optical density data were analyzed using Tukey HSD to compare between groups. Results: The BHK-21 cells viabitlity of SAFC group is greater than the CFC, , indicated by the optical density SAFC (value=0.1233) and CFC (value=0.0936). Conclusion: The viability of BHK-21 cells exposed to SAFC is higher than that of CFC.

The differences between mangosteen peel extract irrigant and NaOCl 2.5% on root canal cleanliness Cornelia Melinda Adi Santoso; Karlina Samadi; Eric Priyo Prasetyo; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Background: Preparation, one of the stages in root canal treatment, always producesdebrisas aresultof instrumentation onroot canalwalls. Debrisconsistsoforganicand inorganicmaterials. Irrigation need to be performed in every preparation so that debrisandmicroorganisms can be removed from root canal wallsthrough flushingmechanism. NaOCl 2.5% is the most popular irrigant used in root canal treatment. However, NaOCl2.5% onlyworks onorganic tissue. Mangosteenpeel extractcontainsvariousactive compounds, such as saponin. Saponin acts assurfactantso as tolower thesurface tension and remove debris from the root canalwalls. Purpose: to compare the cleanliness of root canal walls following irrigation with NaOCl 2.5% and mangosteen peel extract. Method: Eighteen mandible premolar extracted for orthodontics necessity were used in this study. The teeth were divided into three groups, in which each group consists of six teeth. All of them were instrumented with ProTaper for Hand Use and irrigated. Group 1 used aquadest as irrigant, group 2 used NaOCl 2,5%, and group 3 used mangosteen peel extract 400 ug/ml. The roots were split longitudinally into halves and in 1/3 of apex. The surface of the canal walls were examined using scanning electron microscope. Photomicrographs were scored by three independent observers and statistically tested. Result: There were significant differences between three groups (p<0.05). Group 3, irrigated with mangosteen peel extract 400 ug/ml, had the smallest median score that was 1. Conclusion: Mangosteen peel extract is moreeffective thanNaOCl 2.5% incleaningroot canalwall from debris.

Perbedaan Daya Antibakteri Ekstrak Temulawak (Curcuma xanthorrhiza Roxb.) 3,125% dan Chlorhexidine 0,2% terhadap Lactobacillus acidophilus Nanik Zubaidah; Devi Eka Juniarti; Firza Basalamah
Conservative Dentistry Journal Vol. 8 No. 1 (2018): January - June
Publisher : Universitas Airlangga

Background: Caries is a process of demineralization of hard tissues of teeth due to the activity of bacterial metabolism. Lactobacillus acidophilus as one of the cariogenic bacteria that important process of deep caries. Efforts that can be used to inhibit cariogenic bacteria for example using antibacterial agents such as chlorhexidine that have been shown to inhibit Lactobacillus acidophilus bacteria, but chlorhexidine has some side effects. It takes basic herbal ingredients as an alternative agent that can inhibit the growth of cariogenic microorganisms, one of them is temulawak extract. Purpose: This research was conducted to compare antibacterial agent between Curcuma xanthorrhizaRoxb and chlorhexidine 0,2% to inhibit Lactobacillus acidophilus. Methods: The research was a laboratory experimental study. Temulawak extract was made by maceration method with etanol 96% and certain dilution was performed to obtain concentration of temulawak extract 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, 0,781%. A preliminary study was conducted to determine the value of KHM and KBM of temulawak extract against Lactobacillus acidophilus were known by counting the growth of bacteria colonies on muellerhinton media in CFU/ml. From the calculation of the number of colonies Lactobacillus acidophilus, obtained KHM value of 3.125% and KBM value of 6.25%. Further research is conducted by comparing antibacterial ability of temulawak extract used KHM concentration and chlorhexidine 0,2% by using diffusion method by measurement of inhibiton zoneResult: Measurement result of inhibition zone by using calipers show that chlorhexidine 0,2% have inhibiting zone (in mm) 15.1; 15.2; 15.4; 14.9; 15.0; 15,15; 15.5; 15.7; 15.8; 16.0; 14.6; 15.2; 14,85; 15,1; 15.3; 15,2, while the measurement of inhibition zone of temulawak extract 3,125% are (in mm) 10,0; 10.3; 10.2; 9.9; 9.8; 10.1; 10.3; 10.1; 10.45; 10.1; 9.95; 9.85; 8.85; 9.35; 9.45; 9.4. Conclusion: Chlorhexidine 0,2% more potential as antibacterial agent than temulawak extract 3.125% to inhibit Lactobacillus acidophilus.

The Duration Effectivity of Diode Laser 405 nm with Erythrosine Photosensitizer in Killing Streptococcus Mutans Sri Kunarti; Anin Dita; Widya Saraswati
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Introduction: Dental caries is a multifactorial disease caused by the interaction of pathogenic bacteria, especially Streptococcus mutans. Various caries prevention chemically and mechanically can help reduce the number of bacteria. However, this approach does not cover the entire bacterial population with a photodynamic therapy approach with the addition of photosensitizers and low-intensity light radiation with the right wavelength able to eliminate the number of S. mutans bacteria. Purpose:To determinethe duration of effective irradiation time on a 405 nm diode laser with erythrosine photosensitizer in killing S. mutans bacteria.Methods: This research was an experimental laboratory type with 25 S. mutans samples divided into 5 groups treated with erythrosine photosensitizer 0.1 mg / ml and 405 nm diode laser irradiation with 45 seconds, 60 seconds, 75 seconds duration and no erythrosine photosensitizers and without irradiation. The growth of S. mutans bacteria was calculated then the data were analyzed statistically.Results:the research found that S. mutans bacteria decreased gradually in each group; erythrosine without photosensitizer and without irradiation had the highest average growth rate of 71.6 CFU / ml; without photosensitizer with irradiation of 40.6 CFU / ml; irradiation with photosensitizer 45 seconds at 20.6 CFU / ml; irradiation with 60 seconds photosensitizer at 11 CFU / ml; and irradiation with 75 seconds photosensitizer at 0 CFU / ml. In statistical tests, the data are normally distributed and homogeneous. There are significant differences between groups.Conclusion: The effective duration in this study was found at 75 seconds.

Daya Antibakteri Ekstrak Daun Belimbing Wuluh (Averrhoa bilimbi linn) terhadap Bakteri Enterococcus faecalis (Antibacterial Activity of Averrhoa bilimbi linn Leaf Extract against Enterococcus Faecalis) Ranggi Hardian Nugro Astuti; Karlina Samadi; Eric Priyo Prasetyo
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Background. The prevalence of Enterococcus faecalis bacterial infection caused the failure of root canal treatment between 24%-77%. This is due to various factors resistance and virulence of Enterococcus faecalis. This research to find alternative materials that have antibacterial properties and by utilizing natural ingredients that can later be used as a root canal irrigation. Antibacterial activity of the Averrhoa bilimbi linn leaf extract against Enterococcus faecalis bacteria can be determined by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Purpose. The aim of this study was to determine the concentration of Averrhoa bilimbi linn leaf extract that has antibacterial activity against bacteria Enterococcus faecalis. Method. This research is a labolatory experimental with post test only control group design which use diluted Enterococcus faecalis ATCC 29212 according Mc. Farland standard 1,5 x 108 CFU/ml. With treatment Averrhoa bilimbi linn leaf extract on concentration 50%, 45%, 40%, 35%, 30%, and 25% given to each of 0,05 ml Enterococcus faecalis and using Brain Heart Infusion Broth (BHIB) as planting media. Result. At the concentration 30% of Averrhoa bilimbi linn leaf extract, showed that colony’s growth less than 10%. At the concentration 35% was not revealed any bacterial growth. Conclusion. The Averrhoa bilimbi linn leaf extract has antibacterial effect on bacteria Enterococcus faecalis. The MIC was at 30% and MBC was at 35%.

Efek Antibiofilm Glass Ionomer Cements dan Resin Modified Glass Ionomer Cements Terhadap Lactobacillus acidophilus Elsandra Novita Halim; Karlina Samadi; Sri Kunarti
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Background: Risk factors for developing secondary caries are similar to those resulting in primary caries. The marginal seal of a restoration is one of the important factors predicting clinical success. The antibiofilm effect of materials used for the luting cement of oral function affects oral health. Antibiofilm properties of dental luting materials such as Glass Ionomer Cement (GIC) and Resin Modified Glass Ionomer Cement (RMGIC) may improve the restorative treatment outcome. Purpose: This experiment evaluates the antibiofilm effect of GIC and RMGIC on Lactobacillus acidophilus in vitro. Method: Lactobacillus acidophilus served as test microorganism. The quantitative microtiter plate biofilm assays were used to evaluate the antibiofilm effect of the dental luting materials on early-stage biofilm using a direct contact test (DCT) then continued by reading of Optical Density (OD) of biofilm using ELISA reader at a wavelength of 570nm. Result: GIC and RMGIC showed a decrease of OD value from negative control in all groups. The materials’ elute had effect on both bacterial growth with GIC higher then RMGIC to inhibit Lactobacillus acidophilus biofilm formation. Conclusion: The antibiofilm effect of GIC more effective than RMGIC to inhibit Lactobacillus acidophilus biofilm formation.

The effect of theobromine and NaF 2% exposure to enamel surface hardness after immersing in orange juice beverage Tamara Yuanita; Setyabudi Setyabudi; Qintan Sekar Adjani
Conservative Dentistry Journal Vol. 9 No. 2 (2019): July - December
Publisher : Universitas Airlangga

Background: Consumption of orange juice which had acidic quality will cause a demineralization on enamel. The most effective way to prevent the demineralization process was by involving remineralization agents. Fluoride was effective to improve remineralization but has toxicity effect and caused fluorosis at certain dose. Another alternative for remineralization agent without providing side effects is theobromine contained in cocoa peel extract (Theobroma cacao). Objective: To explain the effect of theobromine and NaF 2% exposure on enamel surface hardness after immersion in commercial orange juice. Method: Twenty-seven cattle incisors were cut into a square shape 1x1 cm and then planted in a round resin mold then divided into three groups. All three groups were immersed in orange juice beverage for 60 minutes. The control group was immersed in distilled water, group I was given theobromine 200 mg / L, and group II was given 2% NaF after exposure for 96 minutes. Surface hardness measurements were carried out using the Wolpert Micro Vickers Testers tool. Results: There was a significant difference (p <0.05) between surface hardness of tooth enamel from the control group and theobromine group and there was also a significant difference between the control group and the NaF group. However, there was no significant difference (p> 0.05) in the NaF group with theobromine group. Conclusion: The effect of exposure to theobromine and NaF 2% on surface enamel hardness after immersion in orange juice beverage has the same result.

UJI SITOTOKSISITAS EKSTRAK DAUN MIMBA (Azadirachta indica) TERHADAP SEL FIBROBLAS BHK 21 Qurni Restiani; Mandojo Rukmo; Devi Eka Juniarti
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background: The leaves of neem (Azadirachta indicia) is one of herbal medicine that recommended as an alternative material of root canal irrigants. The active ingredients of neem leaves such as alkaloids, tannins, saponins and flavonoids has been proven to have antibacterial activity against E. faecalis. The ideal properties of an alternative material of root canal irrigants is not only have antibacterial activity but also is not toxic to the tissues, but the toxicity of neem leaves remains unclear until now. Objective: The aims of this study to determine the toxicity effect of neem leaves extract in specific concentration. Methods: This research was an experimental laboratory in vitro study of baby hamster kidney fibroblast (BHK-21). The neem leaves extract was made by maceration method using ethanol 96% and certain dilution performed to obtain various concentrations. Cytotoxicity test was conducted by MTT assay and the optical density was measured using ELISA reader at wavelength of 620 nm. Then, the optical density values were calculated using the formula for determining the number of survival fibroblasts after tested. Results: The percentage of survival fibroblast at concentration of 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95, and 100% in sequence were 71.3%, 64, 2%, 62%, 60.2%, 40.1%, 36.3%, 36.7%, 29%, 28.4%, 27.5%, and 25.6% . Conclusion: The extract of neem leaves (Azadirachta indica) has cytotoxic effects at concentration of 70% up to 100%.

Antibacterial power effectiveness of calcium hydroxide and propolis mixture on Fusobacterium nucleatum bacteria ira Widjiastuti; S. Sukaton; Agnes Melinda Wong; Nanik Zubaidah
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Calcium hydroxide is a root canal dressing material that is widely used in dentistry because of its ability to regenerate hard tissue and eliminate bacteria. According to Ferreira et al. (2015), Fusobacterium nucleatum was found in 90% of teeth that had a root canal treatment done using calcium hydroxide as an intracanal medication. Due to this shortcoming of the antibacterial power of calcium hydroxide, additional research on alternative ingredients that can be combined with calcium hydroxide to improve its antibacterial power is necessary. Propolis is a natural material that has high antibacterial power and has long been used in dentistry. The addition of propolis to calcium hydroxide is expected to improve the antibacterial power of calcium hydroxide without eliminating its function in terms of regenerating hard tissue. Purpose: To find out how effective is the antibacterial power of a combination of calcium hydroxide and propolis against Fusobacterium nucleatum. Methods: The research was carried out using 4 treatment groups consisting of 6 samples for each group. Group 1 is given a combination of calcium hydroxide and propolis with a ratio of 1:1, group 2 with a ratio of 1:1.5, group 3 with a ratio of 1:2, and group 4 is a positive control of calcium hydroxide and sterile aquadest suspension. Each sample was put into a test tube containing BHIB and a suspension of Fusobacterium nucleatum, incubated at 37ºC for 24 hours, and vortexed for 1 minute. A total of 0.1 ml of bacterial inoculum was taken from each sample and then put into the MHA and grown for 24 hours. The number of Fusobacterium nucleatum colonies grown on MHA was calculated and expressed using the Colony Forming Unit (CFU). Results: There were fewer colonies of Fusobacterium nucleatum in the treatment group compared to the control group. Conclusion: The combination of calcium hydroxide and propolis has an effective antibacterial power against Fusobacterium nucleatum which the ratio of 1:2 is more effective than ratio of 1:1,5 and 1:1.

Page 3 of 19 | Total Record : 190

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Editorial Address DEPARTEMEN KONSERVASI GIGI. FAKULTAS KEDOKTERAN GIGI, UNIVERSITAS AIRLANGGA PROF. DR. MOESTOPO 47. SURABAYA. 60132

Location Kota surabaya, Jawa timur INDONESIA

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Dian Agustin W

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cdj@journal.unair.ac.id

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+62315030255

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cdj@journal.unair.ac.id

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DEPARTEMEN KONSERVASI GIGI. FAKULTAS KEDOKTERAN GIGI, UNIVERSITAS AIRLANGGA PROF. DR. MOESTOPO 47. SURABAYA. 60132

Location
Kota surabaya,

Jawa timur

INDONESIA