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IJAAM (Indonesian Journal of Anti-Aging Medicine)
Published by Universitas Udayana
ISSN : 26147882     EISSN : 26155079     DOI : https://doi.org/10.36675/ijaam
Core Subject : Health,
Medicine & Pharmacology
Indonesian Journal of Anti-Aging Medicine is published by Faculty of Medicine Universitas Udayana in collaboration with Indonesian Center for Anti-Aging Medicine (INCAAM). Indonesian Journal of Anti-Aging Medicine is an open access, peer reviewed journal aiming to communicate high quality research articles, reviews and general articles in the field of anti aging. Indonesian Journal of Anti-Aging Medicine encompass all aspects of basic research/clinical studies related to the field of anti aging and allied science fileds. The Journal aims to bridge and integrate the intellectual, methodological, and substantive diversity of medical scholarship, and to encourage a vigorous dialogue between medical scholars and practitioners. The Journal welcomes contributions which promote the exchange of ideas and rational discourse between practicing educators and medical researchers all over the world.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 73 Documents
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Administrations of young betelnut fruit extract (Areca cathecu l.) increased testosteron concentration in old male wistar white rat (Rattus norvegicus) Dessy Indriana Hatta; Wimpie Pangkahila; I Gede Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 1 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Background: With increasing age, testosterone level is tend to and cause sexual dysfunction disorders such as erectile dysfunction, sexual arousal disorders, ejaculatory disorders, orgasm disorders to infertility. Young betel nut extract (Areca catechu L.) is an herbal plant that can increase sexual arousal (libido) which has many nutrients including antioxidants. The purpose of this study was to prove that administration of young areca fruit extract could increase testosterone levels in old male wistar white rats (Rattus norvegicus). Method: This study was an experimental study using a pretest-posttest control group design that used 16 wistar male rats (Rattus norvegicus) aged 18 months old, weighing 225-250 grams divided into 2 (two) groups, namely the control group (Placebo ) and the treatment group who were given young betel nut extract 100 mg / kgBB / day (P1). The study was conducted for 28 days followed by blood sampling for testosterone levels. Results: The results showed significant increase in testosterone levels in treatment group compared to the control group, (p= <0.05)). The testosterone level increased from 14.813 ± 1.343 ng/ml before treatment to 19.602 ± 1.345 ng/ml after treatment. Conclusion: Based on the results of the analysis it can be concluded that the administration of Areca catechu L. extract can increase Testosterone levels in old male wistar white rats (Rattus norvegicus).
The Aplication of Topical Wharton’s Jelly Mesenchymal Stem Cell Conditioned Medium (Wjmsc-Cm) Inhibit the Increase in Expression of Matrix Metalloproteinase-1 and Increased the Amount of Collagen in the Skin Of Wistar Mice Exposed to Ultraviolet-B Rays Suarni .; Wimpie Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 1 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Introduction: Ultraviolet B exposure is one of the factors causing extrinsic skin aging called photoaging. In this study, stem cell derived media growth factors conditioned from umbilical cord was used. The purpose of this study is to prove that topical administration of Wharton’s Mesenchymal Stem Cell Conditioned Medium Jelly (WJMSC-CM) could inhibit the increase in expression of matrix metalloproteinase-1 and increases the amount of collagen in the skin of wistar rats exposed to ultraviolet-B light. Methods: An animal experimental with Post-test Only control group design study was conducted to evaluate the aforementioned hypothesis. The number of samples used in this study was 36 wistar rats, aged 3-4 months and were divided into 2 groups: the control group consisted of 18 rats and were given placebo glycerin gel and exposed to UV B rays, while the treatment group was given WJMSC-CM and exposed to UV B rays. All treatments were given UV B rays with a total irradiation dose of 840mj / cm2 for 4 weeks. The study was carried out at the Laboratory Animal Unit Unit of Pharmacology, Faculty of Medicine, Udayana University, Denpasar. Results: By using the Shapiro Wilk test for numerical data with normal distribution, Levene’s Test showed that the distribution of data and variants are homogeneous groups for both groups. The results of the comparative analysis of the two groups using independent t-test showed significant differences between the two groups on MMP-1 expression and the amount of collagen (p <0.05). Conclusion: The conclusion of this study is the administration of WJMSC-CM topically inhibits the increase in MMP-1 expression and increases the amount of collagen in wistar rats exposed to UV B light.
Black rice bran (oryza sativa l. indica) extract cream prevented the increase of dermal matrix metalloproteinase-1 and dermal collagen reduction of male Wistar rats (rattus norvegicus) exposed to ultraviolet-B rays Astried Haryanto; Wimpie Pangkahila; Anak Agung Gde Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (499.849 KB) | DOI: 10.36675/ijaam.v4i1.48

Abstract

Introduction: The radiation of UV B rays exposure is capable of inducting free radicals to the skin, damaging the dermal collagen and stimulating skin aging. Photoaging prevention may be conducted by utilizing antioxidant and one of the good sources of antioxidant is black rice bran. Therefore the purpose of this research was to assess the effects of topical 35% black rice bran (Oryza sativa L.indica) extract toward dermal Matrix Metalloproteinase-1 (MMP-1) and dermal collagen in male Wistar rats (Rattus norvegicus). Method: A post-test only control group design was conducted using 36 male Wistar rats divided into 2 groups (treatment and control). Both groups were exposed to UV-B rays 3 times a week for 4 weeks but the treatment group received 35% black rice bran extract cream twice a day. The control group was only treated by base cream. Histopathological examination was used to assess MMP-1 expression while Picro Sirius Red staining was used to assess dermal collagen on the rat’s skin. Result: Comparative statistical analysis results for both groups revealed that MMP-1 expression in treatment group was significantly lower than control group (30.94±4.22% vs. 9.57±2.76%; p<0.001). Meanwhile, collagen density was significantly higher in treatment group (83.59±2.84% vs. 58.40±2.69%). Conclusion: Topical application of 35% black rice bran extract cream prevented the increase of MMP-1 expression and dermal collagen reduction. Further study is needed to validate these findings.
Oral L-Arginine lower neovascularization and the number of fibroblasts but failed to increase nitric oxide and epithelialization in the healing process of male white diabetic wistar rats (Rattus norvegicus) Fenni Liem; Wimpie Pangkahila; I Wayan Weta
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 2 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.194 KB) | DOI: 10.36675/ijaam.v3i2.44

Abstract

Introduction: Oral L-Arginine is a conditional essential amino acid that plays a role in wound healing in DM. The role of arginine in diabetic wounds is by enhancing blood circulation in the injured area and increasing oxygen supply to the wound tissue. The purpose of this study to prove the administration of oral L-Arginine toward vascularization status in wound healing of male white rats wistar diabetes mellitus. Methods: A randomized posttest only control group study using with 36 diabetic induced wistar rats (Rattus Norvegicus) aged 2-3 months and weighing 180-200gram which then divided randomly into two groups. Nitric oxide level was measured on the third day and each group was then further divided into two groups for examination of neovascularization, fibroblasts and epithelialization on the seventh day and on the tenth day. Results: Administration oral L-Arginine failed to induce any significant change in Nitric Oxide level and wound gap closure. On the other hand, the results showed that the mean neovascularization was significantly different between the two groups on the 10th day (Control group vs intervention group: 4.22±1922 vs1.89±1364; p=0.009). In addition, the mean number of fibroblast at the 10th day was also significantly different (Control group vs intervention group: 74.11±28.57 vs 38.11±20.90; p=0.008). Conclusion: In conclusion, oral L-Arginine did not significantly affect nitric oxide and epithelialization while decreased neovascularization and the number of fibroblasts on day tenth in the healing process of male white rats diabetes mellitus
Administration of deep sea shark liver oil reduce malondialdehid (mda) levels on male wistar rats exposed to cigarette smoke Feily Sari Moelyono; Wimpie Pangkahila
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 2 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Introduction: The Deep Sea Shark Liver Oil contains squalene, omega-3, and beta-carotene that could act as effective antioxidant to counter oxidative stress caused by cigarette smoke. This study aims are to prove the effect of Deep Sea Shark Liver Oil on reducing MDA levels in male Wistar rats exposed to cigarette smoke. Methods: This study was a pre and post-test control group experimental study. 20 male Wistar rats were used and divided into 2 groups that were exposed to cigarette smoke for 45 minutes once a day. Group 1 received placebo while group 2 received 140 mg Deep Sea Shark Liver Oil per 200 grams of rat body weight for 14 days. Result: The pre-test MDA level in both groups were comparable and normally distributed (Group 1: 7.71±0.23 nmol/ml; Group 2: 7.57±0.28 nmol/ml). However, post-test result showed that the MDA level in treatment group was significantly lower compared to control (Group 1: 8.45±0.37 nmol/ml; Group 2: 6.34±0.33 nmol/ml; p: <0.05). Conclusion: In conclusion, the administration of 140 mg of Deep Sea Shark Liver Oil per 200 grams of rat body weight for 14 days effectively reduced MDA levels in rats exposed to cigarette smoke.
Ekstrak Teh Putih (Camellia sinensis) Oral Mencegah Dislipidemia pada Tikus (Rattus novergicus) Jantan Galur Wistar yang Diberi Diet Tinggi Lemak Delly Dahlia; Wimpie I Pangkahila; I Gusti Made Aman; J Alex Pangkahila; N. Tigeh Suryadhi; Ida Sri Iswari
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 1 No 1 (2017): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Dislipidemia adalah kelainan metabolisme lipid yang ditandai terjadinya peningkatan kolesterol total, trigliserida, kolesterol LDL dan penurunan kolesterol HDL. Pada saat ini banyak penelitian untuk mencegah dan mengobati dislipidemia dengan bahan alami. Teh putih merupakan teh tanpa proses fermentasi yang berasal dari daun teh (camellia sinensis) yang sangat muda dan masih menggulung serta terlindung dari sinar matahari sehingga mencegah degradasi polifenol. Ekstrak teh putih mengandung derivat katekin tertinggi dibanding teh lainnya , ECGC (Epigalocathecin 3-Gallate) dan kafein ini dapat memperbaiki profil lipid darah dan memiliki efek vasoprotektif, juga memiliki kemampuan untuk menginhibisi (Cholesteryl ester transfer protein) CETP, yang bisa meningkatkan kadar kolesterol HDL dan menurunkan kadar kolesterol total ,trigliserida dan kolesterol LDL. Penelitian ini bertujuan untuk mengetahui kegunaan Ekstrak Teh Putih (camellia sinensis) sebagai alternatif untuk mencegah dislipidemia dan mengetahui dosis pemberian ekstrak teh putih untuk mencegah dislipidemia pada tikus jantan galur wistar. Penelitian ini adalah penelitian eksperimental murni dengan randomized posttest only control group design. Tikus putih jantan dipilih secara random dan dibagi menjadi 3 kelompok, masing-masing berjumlah 10 ekor tikus, yaitu kelompok kontrol diberikan diet tinggi lemak dan plasebo yang berupa akuades , kelompok perlakuan I diberi diet tinggi lemak dan ekstrak teh putih 14,4 mg, dan kelompok perlakuan II diberi diet tinggi lemak dan ekstrak teh putih 28,8 mg masing-masing 1 kali sehari. Setelah perlakuan selama 28 hari sampel darah diambil dari medial kantus sinus orbitalis, untuk pemeriksaan kadar kolesterol total, trigliserida, kolesterol LDL dan kolesterol HDL. Hasil penelitian menunjukkan bahwa pada kelompok pemberian ekstrak teh putih 14,4 mg dan pemberian ekstrak teh putih 28,8 mg terdapat penurunan kolesterol total secara bermakna masing-masing 137,31% dan 156,65% (p < 0,05), penurunan trigliserida secara bermakna masing-masing 77,29% dan 101,01%(p < 0.05) dan penurunan kolesterol LDL masing-masing 53,58 % dan 75,12%(p < 0,05), serta peningkatan kolesterol HDL secara bermakna masing-masing 44,31% dan 66,39% (p < 0,05). Penelitian ini menyimpulkan ekstrak teh putih mencegah peningkatan kolesterol total, trigliserida, kolesterol LDL dan mencegah penurunan kolesterol HDL, jadi ekstrak teh putih mencegah dislipidemia.
Topical administration of deep sea shark liver oil (desslotm) inhibited mmp-1 expression in rat’s skin exposed to ultraviolet-B Bunge Dianmentari; Gede Ngurah Indraguna Pinatih; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.196 KB) | DOI: 10.36675/ijaam.v4i1.39

Abstract

Introduction: Squalene is one of strong antioxidants that can inhibit aging proses. However, its effect on the skin aging is still understudied. Therefore, the purpose of this study was to assess the efficacy of topical administration of Deep Sea Shark Liver Oil toward MMP-1 expression in Wistar rat’s (Rattus Norvegicus) skin exposed to UVB. Methods: An experimental randomized post-test only control group study was conducted using a total of 36 rats Wistar rats were used which were divided into 2 groups. The control group received placebo solution while the treatment group was treated by Deep Sea Shark Liver Oil solution. All groups were exposed to UVB with a total dose of 840 mJ/cm² for 4 weeks. Biopsy was conducted for skin sample collection for the examination expression of MMP-1. Results: Immunohistochemistry evaluation showed that the dermal MMP-1 expression in the control group was higher than in control group. According to the percentage of expression analysis, the mean of MMP-1 expression in control group were significantly higher (29.53%) compared to the treatment group (9.72%) (p=0.001). Conclusion: It can be concluded that the topical administration of Deep Sea Shark Liver Oil solution inhibited the expression of MMP-1 in rat’s skin exposed to UVB.
Administration of bali arabica (Coffea arabica) coffee extracts decreases abdominal fat and body weight in obese wistar rats (Rattus norvegicus) Fanny Rustandi; I Gusti Made Aman; Gde Ngurah Indraguna Pinatih
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 1 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Introduction: The amount of obesity in the world in 2016 tripled compared to 1975 based on data from the World Health Organization (WHO). Previous research has shown that coffee has the potential to reduce weight and heavy abdominal fat. But there has been no research on extracts of Balinese arabica coffee beans. The purpose of this study was to prove that extracts of Balinese Arabica coffee beans can reduce weight and weight of abdominal fat in obese wistar male rats. Methods: This research is a study using Post-test only Control Group Design. The research subjects were 36 male white rats wistar (Rattus norvegicus) strain, healthy, aged 3-4 months and obese (Lee index> 0.3) divided into two groups, each amounting to 18 rats. The control group (P0) was the one who received placebo and the treatment group (P1) was given bali coffee bean extract 800mg / kgBW / day for 28 days. The variables observed were body weight, abdominal subcutaneous fat weight (BLS), and weight of visceral abdominal fat (BLV). In addition, the remaining food is measured every day. Results: The results showed the mean body weight P0 302.33 ± 6.22gram, P1 286.88 ± 4.85 gram (p <0.005). The mean BLS P0 was 0.61 ± 0.07 grams and P1 was 0.41 ± 0.08 grams (p <0.005). The mean BLV P0 5.55 ± 0.90 gram and P1 4.58 ± 0.60 gram (p <0.005). The mean feed remaining from the control group was 4.081 ± 0.57 grams and from the treatment group 6.07 ± 0.64 grams (p <0.005). Conclusion: Based on the above data it can be concluded that the administration of Bali coffee bean extract can reduce weight, weight of abdominal subcutaneous fat and heavy abdominal visceral fat in male wistar rats with obesity. In addition, it is suspected that extract of Bali coffee beans also reduces the amount of food intake.
Intraperitoneal administration of dexpanthenol inhibits the decrease in the number of leydig and sertoli cells in the wistar strain of white rat (rattus norvegicus) test exposed to monosodium glutamate Susianingsih Murni Hartati; Wimpie Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 1 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Introduction: Free radical accumulation that goes beyond the ability of the body’s defense mechanism causes oxidative stress which is one of the causes of premature aging in the testicular organs. Dexpanthenol works by increasing the levels of intracellular coenzyme A, ATP and glutathione which play a role in the defense and repair of cells against oxidative and inflammatory stress. The purpose of this study was to prove that intraperitoneal administration of Dexpanthenol inhibited the decrease in the number of Leydig cells and Sertoli cells in the testes of Wistar rats (Rattus norvegicus) exposed to Monosodium glutamate (MSG). Methods: This research is true experimental with post-test only control group design. 32 rats were divided into two groups with each group consisting of 16 rats. The first group was the Control Group (P1) which was given a placebo aquadest twice a week intraperitoneally. The second group was the treatment group (P2) which was given Dexpanthenol 1000 mg / kgBW mice twice a week intraperitoneally. All groups were given 4 g / kgBB of MSG mice every day with sonde. The experiment lasted 14 days and then testicular tissue was taken be evaluated and the number of Leydig cells and Sertolinya cells were counted. Results: The Saphiro-Wilk test results showed that the data of the number of Leydig cells was not normally distributed (p <0.05) while the data of the Sertoli cell counts were normally distributed p> 0.05. Comparative analysis of Leydig cell counts was conducted using Mann-Whitney Test while comparative analysis of Sertoli cell numbers using Independent-T Test. The results of the analysis showed that there were significant differences in the mean number of Leydig cells and Sertoli cells between groups P1 and P2. Conclusion: This study concluded that intraperitoneal administration of Dexpanthenol inhibits the decrease in the number of Leydig cells and Sertoli cells in the testis of Wistar strain white rats (Rattus norvegicus) exposed by Monosodium glutamate.
Macassar fruit extract (Brucea javanica (l.) merr) increased the level of superoxide dismutase (SOD) but had no effect on the level of malondialdehyde (mda) in paraquat-treated male swiss Webster mice Maria Eka Patri Yuliati; I Gusti Made Aman; Ni Nyoman Ayu Dewi
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 2 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.984 KB) | DOI: 10.36675/ijaam.v3i2.41

Abstract

Introduction: Paraquat exposure causes aging because it induces oxidative stress marked by decreased level of SOD and increased MDA serum level. Macassar fruit contains bioactive compounds such as vitamin C, flavonoids, tannins and polyphenol that have antioxidant activity. The purpose of this study was to prove that Macassar fruit extract increased the level of Superoxide Dismutase (SOD) enzyme and reduce the level of Malodialdehid (MDA) in male Swiss Webster mice treated by paraquat. Methods: A randomized pretest-posttest control group study was conducted using 14 male mice which were 2-3 months old, healthy and had 25-30 gram in weight. They divided into 2 groups namely P0 (control) and P1 (Treatment). Both groups were treated by paraquat but only group P1 received 20 mg Macassar fruit extract while the P0 only got 1 cc placebo for 14 days. Results: Our result showed that the level of SOD was increased in P1 group from 17.18±1.69 U/ml to 67.56±3.65 U/ml (p<0.01) while no change was observed in P0 group 16.97 ±1.45U/ml to 17.07±1.89 U/ml (p>0.05). However, no effect on MDA level was observed as the level of MDA tended to slightly decrease in both groups. Conclusion: It can be concluded that Macassar fruit extract 20 mg/kgBB significantly increased the level of SOD whilehave no effect on MDA level in male mice Swiss Webster treated by paraquat.

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