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IJAAM (Indonesian Journal of Anti-Aging Medicine)
Published by Universitas Udayana
ISSN : 26147882     EISSN : 26155079     DOI : https://doi.org/10.36675/ijaam
Core Subject : Health,
Medicine & Pharmacology
Indonesian Journal of Anti-Aging Medicine is published by Faculty of Medicine Universitas Udayana in collaboration with Indonesian Center for Anti-Aging Medicine (INCAAM). Indonesian Journal of Anti-Aging Medicine is an open access, peer reviewed journal aiming to communicate high quality research articles, reviews and general articles in the field of anti aging. Indonesian Journal of Anti-Aging Medicine encompass all aspects of basic research/clinical studies related to the field of anti aging and allied science fileds. The Journal aims to bridge and integrate the intellectual, methodological, and substantive diversity of medical scholarship, and to encourage a vigorous dialogue between medical scholars and practitioners. The Journal welcomes contributions which promote the exchange of ideas and rational discourse between practicing educators and medical researchers all over the world.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 73 Documents
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Ethanol extract of Neem (azadirachta indica a. juss) twigs peel gel increased neovascularization, fibroblast and epithelialization in wound healing of male albino Wistar rats (Rattus norvegicus) Noviyanti Situmorang; I Gusti Made Aman; Ni Nyoman Ayu Dewi
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i1.54

Abstract

Introduction: Proper wound healing is characterized by increased neovascularization, fibroblast cell number and epithelialization. This process can be accelerated using several natural substances and one of them is Neem (Azadirachta indica A. Juss) twigs extract. However, its wound healing effect is still poorly investigated. Therefore, the aim of this study was to assess the efficacy of ethanol extract of Neem twigs peel gel toward wound healing in male albino Wistar rats (Rattus norvegicus). Methods: A randomized posttest only control group study was conducted using 28 male albino Wistar rats as a subject. Subjects were divided into four groups; the first and second group was the control group which was treated using placebo gel for 4 and 12 days while the third and fourth groups were treated with 12.5% extract gel for 4 and 12 days. On day 4 and 12, rats were euthanized then examined histologically. Results: The results showed a significant increase of neovascularization in the P1.4 compared to P0.4. However, it decreased significantly at day 12. Similarly, the number of fibroblast cells was also increased in the treatment group until day 4 but decreased significantly and even lower than the control group at day 12. On contrary, epithelialization was continuously increased in both group and the treatment groups consistently demonstrated higher epithelialization compared to control. Conclusion: It can be concluded that ethanol extract of Neem twigs peel gel enhanced wound healing process in male albino Wistar rats.
Oral administration of bitter gourd (Momordica Charantia) extract prevent dyslipidemia on male wistar rats (rattus norvegicus) fed with high-fat diet Hendrik Danico; Wimpie Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i1.49

Abstract

Introduction: Dyslipidemia is one of the most prevalent health conditions and often leads to development of many chronic diseases such as coronary artery disease, heart failure, and stroke. This study was conducted to investigate the effects of bitter gourd (Momordica Charantia) extract on lipid profiles in male wistar rats fed with high-fat diet. Methods: This study was an experimental research using randomized post-test only control group design. The subjects were 36 male Wistar rats (Rattus norvegicus) randomly divided into 2 groups. The first group was the control group (P0) and fed with placebo while the treatment group (P1) received bitter gourd (Momordica Charantia) ethanol extract at 1g/kg body weight/day. Both groups were fed with high fat diet. The experiment was lasted for 35 days and lipid profiles were measured at the end of the experiment. Results: Statistical analysis results revealed the mean of total cholesterol, triglyceride, and low-density lipoprotein (LDL) in the treatment group were significantly lower than control group while high-density lipoprotein (HDL) was significantly higher in the treatment group (p<0.05). Conclusion: This study concluded that bitter gourd extract significantly prevent dyslipidemia on male Wistar rats fed with high-fat diet.
Topical virgin red palm oil improved wound healing by increasing fibroblasts, neovascularization and epithelialization in male Wistar rats (Rattus norvegicus) Heidy Dwi Nadia; I Wayan Weta; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i1.50

Abstract

Background: The healing process of burns wound can be accelerated using natural compound and one example of alternative medicine is virgin red palm Oil (VRPO). However, scientific evidence that back this notion is still lacking. Therefore, this study aimed to assess the effect VRPO toward burn wound healing in male wistar rats (Rattus norvegicus). Methods: An experimental randomized post-test only control group study was conducted using 28 male Wistar rats. Burn wound was created in all rats but only treatment group received VRPO while the control group was treated with placebo cream. Skin sample was obtained from each rat at day 4 and 11 for histological examination (fibroblasts density, neovascularization, and epithelialization). Results: The results showed that VRPO improved fibroblast density, neovascularization, and epithelialization at day 4 compared to control group. However, at day 11, there was a decreased in fibroblast density and neovascularization while epithelialization continuously increased at double the rate of the control group. Conclusion: Topical VRPO improved burn wound healing by increasing wound healing speed and epithelialization.
Moringa (Moringa oleifera) leaves extract gel improved wound healing by increasing fibroblasts, neovascularization and in male Wistar rats Citra Anissa Ayu; I Wayan Weta; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i1.51

Abstract

Background: Wound healing can be accelerated by the supplementation of naturalcompound especially flavonoids and tannins. These compounds is known to be abundant in several plant species such as moringa (Moringa oleifera). Therefore, the aim of this study was to assess the effect of moringa leaves extract gel toward wound healing rate in in male Wistar rats (Rattus norvegicus). Methods: This research was a true experimental study using randomized post-test only control group design. Subjects were 28 rats (Rattus norvegicus), males, 5-6 months old, weighing 200-250 gram, which were divided into four groups (n= 7 rats) randomly. The first group was treated with placebo for 4 days (P0(4)), the second group was treated with moringa leaves extract gel for 4 days (P1(4)), the third group was treated with placebo for 11 days (P0(11)), and the fourth group was treated with moringa leaves extract gel for 11 days (P1(11)), and the last of intervention carried out measurements of fibroblast cell , neovascularization and epithelialization. Results: Administration of moringa leaves extract gel significantly increased the fibroblast density, neovascularization, and epithelial thickness. However, despite increased density of fibroblast and neovascularization at day 4, they significantly dropped at the second measurement (day 11). On the other hand, epithelial thickness was consistently improved overtime compared to control. Conclusion: The Moringa oleifera leaves extract gel improved wound healing by speeding healing process and epithelialization in male wistar rats (Rattus norvegicus).
Oral administration of melatonin increased superoxide dismutase and reduced malondialdehyde in male wistar rats (rattus norvegicus) induced by cigarette smoke Aprilany Stella peng; Wimpie Pangkahila; I Wayan Weta
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i1.52

Abstract

Introduction: Cigarette smoke is one of the most common sources of free radicals that accelerate aging process. This process can be countered by avoiding cigarette and consuming anti-oxidants. Melatonin is one of well-known anti-oxidant which can be safely consumed. Therefore, the aim of this study was to assess the effect of oral melatonin supplementation toward the level of superoxide dismutase and malondialdehyde in male Wistar rats exposed by cigarette smoke. Methods: An experimental pretest-posttest control group study was conducted using 18 male Wistar rats, 12 weeks old, and 180-200 gr in weight. The subjects were divided into two groups (n= 9 rats) randomly. Both groups were exposed by cigarette smoke but treatment group (P1) was treated with 2 mg of melatonin per 200 gr BW rats for 14 days. The levels of SOD and MDA were measured using the ELISA method. Results: The baseline level of SOD and MDA in both groups was homogenous. At the end of the experiment, there was significant increase of SOD level (baseline: 1.13±0.45; post-treatment: 2.57±0.08 ng/mL; p= <0.001) and decreased in MDA level (baseline: 9.53 ± 1.25; post-treatment: 1.44 ± 0.46; p= <0.001) in treatment group while no change was observed in both SOD and MDA in control group. Conclusion: The oral consumption of melatonin countered the adverse effect of smoking by increasing the level of SOD and lowers MDA level.
Topical bromelain cream did not increase neovascularization, fibroblast and epithelialization in wound healing of diabetic-rats (rattus norvegicus) Maulidha Suzi Annisa; Wimpie Pangkahila; IGM Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.90

Abstract

Introduction: The diabetic-wound healing process is longer due to blood vessel ischemia. Bromelain is the enzyme that acts as debridement agent with anti-inflammatory effect. The aim of this study was to prove the topical administration of bromelain increase neovascularization, fibroblast, and epithelialization in wound healing of diabetic-rats. Methods: This study used post-test only control group design. Subjects were 32 male Wistar diabetic-rats (Rattus norvegicus. It was divided into 2 groups (n = 16) and given amoxiciline for 3 days. The control group consist of: O1 group treated with a base cream for 5 days and O2 group was treated for 12 days. While the treatment group consist of: O3 group was treated with 70% bromelain cream for 5 days and O4 group was treated for 12 days. The neovascularization, fibroblasts, and epithelialization were examined histopathologically. Results: The mean number of fibroblasts in O1 and O3 were 222.88±37.94 cells/field of view and 25.38±7.94 cells/field of view, while O2 and O4 were 99.63±82.33 cells/field of view and 32.25±12.453 cells/field of view (p <0.001). The mean number of neovascularization in O1 and O3 were 15.13±1.95 and 2.88±1.80 cells/field of view, while O2 and O4 were 5.50±3.78 cells/field of view and 1.50±1.06 cells/field of view (p <0.001). The epithelial gap size in the O1 and O2 group were 377.56±573.88 μm and 229.04±647.83 μm (p <0,001). Both O3 and O4 groups showed no longer a wound gap (p>0,05). Conclusion: Topical bromelain cream 70% did not increase neovascularization, fibroblast, and epithelialization in wound healing of diabetic-rats.
Administration of Oral Sorghum Seed Extract (Sorghum bicolor L.) Increased Pancreatic Beta Cells Count but did not Reduce Glycated Albumin Levels in Male Wistar Rats (Rattus Norvegicus) with Diabetes Mellitus Efrida Sumawati; Wimpie Pangkahila; A.A. Gede Budhiarta
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.91

Abstract

Background: It is required a long time of diabetes mellitus treatment, such as doing general activities or even insulin. Sorghum is a plant that contains antioxidants. The aim of this research was to prove the oral administration of Sorghum Seed Extract (Sorghum bicolor L.) in increasing the pancreatic beta cells count but did not reduce the levels of glycated albumin in male Wistar rats (Rattus norvegicus) with Diabetes Mellitus. Method: This study used experimental post-test only group design. 36 male Wistar rats were used and injected with the nicotinamide and streptozotocin intraperitoneally. Rats were divided into 2 groups, the control group (n=18) was administrated with distilled water at a dose of 2cc/200g body weight of rats. The the treatment group (n=18) was administrated with ethanol extract of sorghum seeds with a dose of 80 mg/kgBW of rats/ day. The post-test of glycated albumin levels and histopathology examination of pancreatic beta cells count were performed. Results: The mean of pancreatic β cells count in the control group was 64.31 ± 19.397. It was lower than the treatment group which was 119.44 ± 33.799 (p <0.005). The mean results glycated albumin level of the research in the control group was 8.577 ± 3.105 ng/ml and in the treatment group was 9,191 ± 3,014 ng/ml (p> 0,05), therefore it was not proven. Conclusion: The administration of ethanol extract of sorghum seeds increased pancreatic β cells count, but it did not reduce glycated albumin levels in male Wistar rats (Rattus Norvegicus) with Diabetes Mellitus.
Administration of Etanol White Turmeric (Curcuma zedoaria) Extracts Increased Superoxide Dismutase (SOD) And Decreased Malondialdehide (MDA) In Cigarette Smoke-Exposed Male Mice (Mus musculus) Strain Balb/C Yuliana Tjua; Ida Sri Iswari; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.92

Abstract

Background: Cigarette smoke is free radical which decrease superoxide dismutase (SOD) and increased malondialdehyde (MDA) levels. Etanol white turmeric (Curcuma zedoaria) extract contains flavonoids, polyphenols, tannins, and antioxidant activity. This study aimed to prove white turmeric increased SOD and decreased MDA in cigarette smoke-exposed male mice (Mus musculus) strain Balb/c. Methods: This study used a randomized pre-test and post-test control group design. The subjects were 12 male mice and divided into two groups (n = 6). The control group was exposed to cigarette smoke 1 stick/day and given aquadest 1 ml as a placebo. The treatment group was exposed to cigarette smoke 1 stick/day and given white turmeric extract of 4 mg/20gram BW in one-hour cigarette smoke exposure. The blood was drawn through canthus medial sinus orbital to examine the level of SOD by ELISA and MDA level by TBARS method. Results: There was no significant differences in SOD levels (p = 0.930) and MDA levels (p= 0.984) between groups in pre-test. However, the mean SOD levels (p <0.001) and MDA levels (p<0.001) between groups were significant in post-test. In control group, there was no change in SOD (from 0.555±0.156 to 0.595±0.233 ng/mL; p = 0.650) and MDA (from 14.3±0.614 to 14.3±0.513 μM; p = 0.958). In contrast, the treatment group has increase SOD (from 0.565±0.273 to 2.28±0.110 ng/mL; p <0.001) and decrease MDA (from 14.3±0.931 to 5.78±1.06 μM; p <0.001). Conclusion: Administration of Etanol white turmeric extracts increased SOD and decreased MDA in cigarette smoke-exposed male mice strain Balb/c.
Administration of Binahong (Cordifolia (Ten) Steenis Anredera) Leaves Extracts Reduced Body Weight and Visceral Abdominal Fat Mass in Obese Male Rats (Rattus norvegicus) With Moderate Physical Exercise Valentine Retny Shiajanto; Alex Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.57

Abstract

Introduction: Overweight and obesity are abnormal conditions characterized by excessive fat accumulation in adipose tissue, to the extent that health is impaired. It is associated with a greater risk of various metabolic diseases. Binahong (Cordifolia (Ten) Steenis Anredera) leaves extract is known to contain flavonoids, saponins, and tannins that have an effect on weight loss and a decrease abdominal fat mass. This study aims to prove that the administration of binahong leaves extracts reduces body weight and visceral abdominal fat mass in obese male rats (rattus norvegicus) with moderate physical exercise. Methods: This research was a true experimental study with the post-test only control group design. The research subjects were 36 male rats divided equally into 2 groups, (18 rats each). The positive control group (P1) were treated with moderate physical exercise (20 min of swimming/day for 2 weeks) and oral aquadest (placebo) for 2 weeks, while the treatment group (P2) were treated with moderate physical exercise (20 min of swimming/day for 2 weeks) and oral binahong leaves extract of 400 mg/kg bW once a day for 2 weeks. Body weight and abdominal visceral fat mass were measured after 2 weeks of treatment. The daily remaining feed was observed to observe the food intake. Results: The results showed that the average body weight of P1 group was 288.6±6.11 g, whereas the P2 group was 272±6.48 g (p<0.001). The abdominal visceral fat mass in the P1 group was 9.24±0.48 g, while in the P2 group was 7.43±0.58 g (p<0.001). The median remaining feed in group P1 was 3 g, while in the P2 group was 9.54 g (p<0.001). Conclusion: Together, the results of this study suggested that binahong leaves extracts reduced body weight and visceral abdominal fat mass in obese male rats (rattus norvegicus) with moderate physical exercise.
The application of black garlic (Allium sativum linn) extract cream in preventing the increase level of MMP-1 expression and the decrease of dermal collagen count in the skin of male wistar rats (Rattus norvegicus) exposed to UV-B Rays Balqis Wasliati; Wimpie Pangkahila; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.81

Abstract

Introduction: UV-B exposure triggers the activation of Protein-1 (AP-1) which disrupts the integrity of the extracellular matrix by inducing MMP-1. Black garlic causes an increase of antioxidant content namely phenolic, flavonoid (quercetin), and DPPH (IC-50). This study aimed to determine the application of black garlic (Allium sativum linn) extract cream in preventing the increased level of MMP-1 expression and the decrease of dermal collagen count in the skin of male Wistar rats exposed to UV-B rays. Methods: This study employed an experimental Post Test Only Control Group Design using 32 male Wistar rats. The rats that were applied with base cream material and exposed to UV-B rays were called the control group. The rats that were applied with 15% black garlic extract cream and exposed to UV-B rays were called the treatment group. The expression of MMP-1 was examined by immunohistochemistry and the dermal collagen count of the skin using Pico-Sirius-Red staining. Results: The mean expression of MMP-1 of the control group was 28.58% and the treatment group was 8.60% (p <0.001). The mean count of collagen in the control group was 57.53% and the treatment group was 83.80% (p <0.001). There was a significant difference in the MMP-1 expression and the count of collagen between the treatment of the control group and the treatment group. Conclusion: Administration of the cream of 15% black garlic extract was proven to prevent an increase MMP-1 expression and decrease dermal collagen count in the skin of male Wistar rats exposed to UV-B rays.

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