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IJAAM (Indonesian Journal of Anti-Aging Medicine)
Published by Universitas Udayana
ISSN : 26147882     EISSN : 26155079     DOI : https://doi.org/10.36675/ijaam
Core Subject : Health,
Medicine & Pharmacology
Indonesian Journal of Anti-Aging Medicine is published by Faculty of Medicine Universitas Udayana in collaboration with Indonesian Center for Anti-Aging Medicine (INCAAM). Indonesian Journal of Anti-Aging Medicine is an open access, peer reviewed journal aiming to communicate high quality research articles, reviews and general articles in the field of anti aging. Indonesian Journal of Anti-Aging Medicine encompass all aspects of basic research/clinical studies related to the field of anti aging and allied science fileds. The Journal aims to bridge and integrate the intellectual, methodological, and substantive diversity of medical scholarship, and to encourage a vigorous dialogue between medical scholars and practitioners. The Journal welcomes contributions which promote the exchange of ideas and rational discourse between practicing educators and medical researchers all over the world.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 73 Documents
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The relationship between the levels of adiponectin HMW and the metabolic syndrome component in menopausal women Melanie Maria Magdalena Ratulangi; Anak Agung Gede Budhiarta; Wimpie Pangkahila
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 1 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Introduction: In menopausal women, there increasing evidence of adipocyte dysfunction which result in a decrease in adiponectin HMW. The decrease in adiponectin HMW causes various pathological conditions related to aging such as metabolic syndrome. Simple adiponectin HMW measurements can evaluate early insulin resistance, metabolic disorders, or vascular health. The purpose of this study was to prove the relationship between adiponectin HMW levels and metabolic syndrome components in menopausal women. Methods: In this study, 45 postmenopausal women were used who declared physically healthy, met the criteria of metabolic syndrome based on IDF (International Diabetes Federation), willing to participate in the study and willing to fill out research forms as research subjects. This research was conducted at Panti Werdha Theodora in Makassar while the examination of HMW Adiponectin, fasting blood glucose, triglycerides, and High Density Lipoprotein was carried out in Prodia Laboratory. Result: The results showed a strong correlation between adiponectin HMW and metabolic syndrome components which included waist circumference, HDL levels, triglyceride levels, systolic blood pressure, diastolic and fasting blood glucose (p <0.05). Conclusion: It can be concluded that there was a relationship between adiponectin HMW levels and the metabolic syndrome component in menopausal women. HDL and waist circumference were the most significant factors compared to other metabolic factors associated with Adiponectin HMW.
Pemberian Krim Ekstrak Metanolik Buah Delima Merah (Punica granatum) Menghambat Penurunan Jumlah Kolagen Dermis Kulit Mencit (Mus gusculus) Yang Dipapar Sinar Ultraviolet B Ariana Suryadewi Soejanto
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 1 No 1 (2017): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Photoaging adalah penuaan kulit ekstrinsik yang disebabkan oleh paparan kronis sinar ultraviolet. Photoaging terutama ditandai timbulnya kerutan yang terjadi akibat kerusakan kolagen oleh reactive oxygen species (ROS) yang terbentuk selama paparan sinar ulraviolet. Proses ini dapat dicegah dengan pemberian antioksidan, baik topikal maupun sistemik. Buah delima merah (Punica granatum) memiliki kandungan vitamin C, asam amino, dan komponen fenol yaitu flavonoid dan tannin (jenis elagitanin) yang dapat berperan sebagai antioksidan. Tujuan penelitian ini adalah untuk mengetahui peran pemberian krim ekstrak metanolik buah delima merah 20% dalam menghambat penurunan jumlah kolagen dermis kulit mencit (Mus musculus) yang dipapar sinar ultraviolet. Penelitian ini merupakan penelitian eksperimental murni dengan menggunakan randomized post test only control group design. Penelitian ini menggunakan 36 ekor mencit yang dibagi menjadi 2 kelompok dan masing-masing kelompok terdiri dari 18 ekor mencit. Kedua kelompok diberi paparan sinar UVB selama 4 minggu dengan dosis total penyinaran sebesar 840 mJ/cm2. Kelompok 1 adalah kelompok kontrol, diberi perlakuan pengolesan plasebo (bahan dasar krim). Kelompok 2 adalah kelompok perlakuan, diberi perlakuan pengolesan krim ekstrak metanolik buah delima merah. Selanjutnya dilakukan pengambilan sampel lapisan kulit mencit dan dibuat sediaan histologis untuk kemudian dilakukan pengecatan dengan reagen Sirius Red. Jumlah kolagen dermis pasca perlakuan (post test) pada kelompok kontrol dan perlakuan dinilai secara kuantitatif dari hasil foto kolagen yang diberi pewarnaan Sirius Red dengan metode analisis digital. Hasil penelitian menunjukkan rerata jumlah kolagen kelompok kontrol adalah 45,71±11,36% dan rerata jumlah kolagen kelompok perlakuan adalah 57,22±10,72%. Analisis kemaknaan dengan uji t-independent menunjukkan bahwa nilai p = 0,004. Hal ini berarti bahwa kedua kelompok sesudah diberikan perlakuan pengolesan krim, rerata jumlah kolagennya berbeda secara bermakna (p < 0,05). Dapat disimpulkan dari penelitian ini bahwa krim ekstrak buah delima merah dapat menghambat penurunan jumlah kolagen dermis kulit mencit yang dipapar sinar ultraviolet B.
Combination treatment of metformin and red fruit (Pandanus conoideus l.) extract increased pancreatic β cell density but had no effect toward fasting blood glucose and glycated albumin levels in diabetic male wistar rats (Rattus norvegicus) Ingrid Rotinsulu; Wimpie I. Pangkahila; A.A. Gede Budhiarta
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 2 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (586.703 KB) | DOI: 10.36675/ijaam.v3i2.45

Abstract

Background: Diabetes mellitus (DM) is a degenerative disease associated with premature aging characterized by hyperglycemia. Hyperglycemia in people with DM causes oxidative stress and increases Glycated Albumin level, which is the initial precursor to the formation of AGEs. In turn, AGEs will lead to pancreatic β cell damage and apoptosis. Red fruit (Pandanus conoideus L) contains phytochemicals with antioxidant that has the potential to reduce diabetic complications. Therefore, this study aimed to evaluate the effect of metformin and red fruit extract combination toward blood glucose, glycated albumin and pancreatic β cells density in diabetic rats (Rattus norvegicus). Methods: A post-test only control group study was conducted using 36 male Wistar rats as subject. All subjects were induced for type-2 DM with Streptozotocin and Nicotinamide. The diabetic rats were then divided into 2 groups: the positive control group treated with metformin + placebo and the treatment group treated with metformin + red fruit extract. Pancreatic β cell, fasting blood glucose and glycated albumin assessments were performed after 21 days of treatment. Results: The results showed the mean number of pancreatic β cells in the treatment group was higher than the control group (116.11±33.14 vs 83.20±23.94 cells/visual field; p=0.002). But the mean fasting blood glucose in the treatment group was not significantly different compared to the control group (107.67±14.93 vs. 113.67±11.19 mg/dl; p=0.181). The same result also found in the gycated albumin level (Treatment vs control: 6.97±3.63 vs 6.42±4.01 ng/ml; p=0.666). Conclusion: It can be concluded that the administration of red fruit extract increased the density of pancreatic β cell but did not reduce fasting blood glucose and glycated albumin levels in diabetic Wistar rats.
Administration of oral tranexamic acid is equally effective with intradermal injections in reducing the amount of melanin in female marmots (Cavia porcellus) exposed to ultraviolet-b Ni Luh Putri Astini; Wimpie Pangkahila; AAGP Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 2 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Introduction: Oral studies of tranexamic acid significantly reduce the MASI (Melasma Area and Severity Index) value but have not evaluated a decrease in the amount of melanin. The purpose of this study is to prove that oral tranexamic acid can reduce the amount of melanin and have the same effectiveness as intradermal injections in female guinea pigs exposed to ultraviolet B. Method: A posttest only control group design study was conducted using 32 female mice, aged 6-8 weeks, weight 250-280 grams, which were randomly divided into 2 groups. Group 1 was given an intradermal injection of tranexamic acid 5 mg/ml, 0.5 ml/cm and exposure to UVB. Group 2 was given oral treatment of tranexamic acid 250 mg twice a day and exposure to UVB. The treatment was given for 4 weeks. Total UVB exposure was 390mj/sec. The study was conducted in the animal laboratory of the Udayana medical faculty. Results: The statistic result showed that the data were normally distributed and homogenous. The comparative test with the independent sample t-test indicated that there was no significant difference between intradermal injections with oral tranexamic acid administration in reducing the amount of melanin in female guinea pigs exposed to UVB with p> 0.05. Conclusion: oral tranexamic acid significantly reduced the amount of melanin and had the same effectiveness as intradermal injections in reducing the amount of melanin in female guinea pigs exposed to UVB.
Oral administration of palm oil (elaeis) increases abdominal fat more than oral administration of coconut oil (cocos nucifera) in wistar male rats Cynthia Sofyanti Sugiharto; I Gusti Made Aman; Ida Sri Iswari
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 1 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Introduction: Coconut oils contain many medium-chained fatty acids (MCFA) which are quickly oxidized to produce more energy than long-chained fatty acids (LCFA) in palm oil which is to be accumulated as body fat. The purpose of this study was to determine that oral administration of palm oil (Elaeis) in wistar male rats increased abdominal subcutaneous fat and visceral fat compared to coconut oil (Cocos nucifera). Methods: An experimental post-test only control group study was conducted using 30 male Wistar rats, 2.5-3 months old, with body weight 130-135 grams, which then treated with distilled water in [Control group (P0)], palm oil [Treatment group 1] (P1)] and coconut oil [Treatment group 2 (P2)] with a dose of 2x0.4 ml daily for 45 days. After treatment, the rat was dissected and the visceral abdominal and subcutaneous fats were weighted. All data were analyzed by One Way ANOVA test. Result: According to post-test analysis, the mean weight of abdominal subcutaneous fat was found at 1.18 ± 0.27 grams, 1.60 ± 0.20 grams, and 0.90 ± 0.36 grams in groups P0, P1 and P2 respectively. The mean visceral fat weight was 0.88 ± 0.45 grams, 1.46 ± 0.34 grams, and 0.69 ± 0.35 grams in groups P0, P1 and P2 respectively. One Way ANOVA test analysis found that there were significant differences in mean weight of visceral fat and abdominal subcutaneous fat between the three groups (p <0.05). Conclusion: It was concluded that, with the same dose and timeframe, the administration of oral palm oil in Wistar male rats has more tendency to increase abdominal fat than coconut oil.
Topical administration of broccoli flower (brassica oleraceae l. Var italica plenck) extract cream 10% prevented increased of melanin in UVB exposed guinea pig (cavia porcellus) Tambor Tiur Marlina; Wimpie Pangkahila; AAGP Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.727 KB) | DOI: 10.36675/ijaam.v4i1.47

Abstract

Introduction: Skin aging is primarily caused by increased free radicals and decreased cellular anti-oxidant system in keratinocyte. Broccoli flower contains several important antioxidant molecules that can inhibit aging process of the skin. Therefore, the aim of this research is to prove the effectivity of administration broccoli flower extract cream on inhibiting the elevation of melanin level on guinea pig (Cavia porcellus) exposed with UV-B. Methods: An animal experiment with post-test only controls group design was conducted using 36 guinea pigs which were divided into 2 groups. The control group was treated with placebo cream while treatment group was treated with 10% broccoli flower extract cream. All groups were exposed with UV-B with dose at 390 mJ/cm2 for 2 weeks and biopsy was taken to examine the level of melanin. Results: The results showed that the average amount of melanin expression in the control group (P0) was 21.48 ± 1.48% while it was 1.32 ± 0.85% in treatment group (P1). Independent T-test analysis showed that the difference was statistically significant (t = 33.95; p = 0.001). Conclusion: It can be concluded that the topical administration of broccoli flower extract cream prevented increase in skin melanin in UV-B exposed guinea pig.
Administration of broccoli extract (Brassica oleracea var. italica) inhibited the increase of malondialdehyde level and the decrease of aortic endothelial cells in male wistar rats (Rattus norvegicus) exposed by cigarette smoke Hayatul Firi; I Gusti Made Aman; Gde Ngurah Indraguna Pinatih
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 1 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Introduction: In the environment, there are many free radicals for the body, one of them is cigarette smoke which can cause oxidative stress conditions and result in damage to body cells. The broccolis extract containing flavonoids, glucosinolates, vitamins C, E and beta-carotene which have a high antioxidant protective effect. Methods: An experimental post-test only control study was conducted using 36 males Wistar rats, aged 10-12 weeks, and 200-300 gr weight which were divided in 2 groups (control and treatment group). The broccoli extract (120 mg/ 200 grBB) was given to treatment group one hour before exposure to cigarette smoke. After 28 days of the treatment, the rats were taken for examination of MDA levels and underwent a surgery for aortic tissue for endothelial histopathology examination. Results: The Finding indicated that the data were in normal distribution through data analysis of the Shapiro Wilk test. Lavene’s test showed that both the data distribution and variety of both groups was homogenous (p>0.05). Through independent t-test, there were significant differences between the two groups on the value of MDA and aortic endothelium number (p <0.05) as the result of the comparison result of both groups. Conclusion: The administration of broccoli extract (Brassica oleracea var.italica) inhibits the increased levels of blood malondialdehyde and a decreased of aortic endothelial cells in Wistar male rats exposed to cigarette smoke.
Cream of leaf pare extract (momordica caharantia l) 4% takes the improvement of effective melanin numbers with 4% hydrocuinon cream on marmut skin (cavia porcellus) heart which uses uvb ultraviolet light Mery Merysia; Wimpie Pangkahila
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 2 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Introduction: Bitter melon leaves is one of the plants that can be used as an antioxidant and antimelanogenic. The active compounds in bitter melon leaves are flavonoids, phenolic acids, tannins and vitamin C. The purpose of this study was to determine the effectiveness of 4% pare (Momordica charantia) leaf extract cream with 4% hydroquinone cream in inhibiting the increase of melanin in marmot skin (Cavia porcellus) which was exposed to ultraviolet light B. Method: This study used experimental study with a randomized post-test only control group design method. This study used 36 male guinea pigs (Cavia porcellus), local strains, 3 months of age with a body weight of 300-350mg, divided into 2 groups, with 18 individuals each. The first group was received 4% hydroquinone cream and exposed to ultraviolet B while the second group received 4% bitter melon extract (Momordica charantia) and also exposed to ultraviolet light B 3x a week with a total irradiation dose of 390 mJ/cm2 for 2 weeks. Results: The results showed that the control group (P0) had a mean number of melanin 0.98 ± 0.25% pixel and the treatment group (P1) had an average number of melanin 1.17 ± 0.34% pixels. The difference was analyzed by t-independent test and found to be insignificant (p = 0.06). Statistical analysis found no significant differences in the number of melanin between the control group and the treatment group (p = 0.06). Conclusion: The results showed that the 4% ethanol extract of pare leaves (Momordica charantia) was as effective as hydroquinone cream in inhibiting the increase in the amount of melanin in the skin of male guinea pigs (Cavia porcellus) exposed to UVB rays.
Pemberian Krim Ekstrak Teh Hijau (Camellia sinensis) Dapat Mencegah Penurunan Jumlah Kolagen Dermis dan Peningkatan Kadar Matriks Metalloproteinase-1 pada Mencit Balb -C Yang Dipapar Sinar Ultraviolet B Heny Widiyowati S; Wimpie I Pangkahila; A.A.G.P. Wiraguna; J Alex Pangkahila; I Nyoman Adiputra; IGM. Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 1 No 1 (2017): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Ekstrak Teh Hijau mengandung polifenol utama dalam daun teh, yaitu katekin yang terdiri dari Epigallocathecin 3-gallate (EGCG), epigallocathecin (EGC), epicathecingallate (ECG) , epicatechin (EC), gallocathecin (GC). Diantara keempat komponen tersebut EGCG (Epigallocathecin Gallate) merupakan komponen yang paling potensial. Polifenol teh hijau memiliki efek peredaman terhadap ROS (Radical Oxidative Superoxide), sehingga dapat mencegah kerusakan kulit akibat dari sinar UV-B. Penelitian ini bertujuan untuk membuktikan pemberian krim ekstrak teh hijau 70% dapat mencegah penurunan jumlah kolagen dermis dan peningkatan ekspresi matriks metalloproteinase-1 pada mencit BALB-C. yang dipapar sinar ultraviolet-B.Penelitian ini adalah penelitian eksperimental laboratorium, menggunakan rancangan the randomized post-test only control group. Sebanyak 30 ekor mencit balb/c yang digunakan pada penelitian ini dibagi menjadi 3 kelompok yang masing-masing terdiri dari 10 ekor mencit. Kelompok Kontrol (O) tidak diolesi apapun, Kelompok 1 diolesi bahan dasar krim (Kelompok Perlakuan 1), dan Kelompok 2 diolesi krim ekstrak teh hijau 70% (Kelompok Perlakuan 2). Semua Kelompok Perlakuan diberikan paparan sinar UVB dengan dosis total 840 mJ/cm2 selama 4 minggu, kemudian dilakukan biopsi untuk permeriksaan jumlah kolagen dan ekspresi MMP-1. Data dianalisis menggunakan one way Anova untuk mengetahui adanya perbedaan signifikan pada ketiga kelompok perlakuan kemudian dilanjutkan uji post hoc dengan menggunakan tes LSD (Least Significance Difference) untuk mengetahui beda nyata terkecil pada taraf kemaknaan <0,05. Hasil penelitian menunjukkan terjadinya penurunan yang lebih rerata kolagen yang bermakna pada Kelompok Kontrol (47,83%) dan Kelompok Perlakuan 1 (50,73%) setelah diberikan paparan sinar UV-B dibandingkan dengan rerata kolagen pada Kelompok Perlakuan 2 (67,59%). Hasil rerata ekspresi MMP-1 terjadinya peningkatan pada rerata kontrol (66,76%) dan rerata Kelompok Perlakuan 1 (74,46%) dibandingkan rerata ekspresi MMP-1 Kelompok Perlakuan 2 (39,31%). Melalui uji post hoc tidak ada perbedaan hasil dari Kontrol dan Kelompok Perlakuan 1 baik pada jumlah kolagen dan ekspresi MMP-1 (P>0,05).Simpulan penelitian ini adalah krim ekstrak teh hijau 70% dapat mencegah penurunan jumlah kolagen dermis dan peningkatan ekspresi MMP-1 pada mencit Balb/C yang diberi paparan UV-B.
Administration of Creatine Monohydrate® increased the estrogen levels but have no effect on testosterone levels in male albino rats (rattus norvegicus) with moderate physical activity Ursula Sulistiani; Wimpie Pangkahila; Alex Pangkahila
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 2 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.79 KB) | DOI: 10.36675/ijaam.v3i2.46

Abstract

Background: Our laboratory assessment indicated that the Creatine Monohydrate® supplement contains phytoestrogen and phytoprogesteron. This study was aimed to prove that the administration of Creatine Monohydrate® supplement could increase the estrogen levels and decrease testosterone levels in male albino rats (Rattus norvegicus) with moderate physical activity given. Methods: A true experimental with pre-test and post-test randomized control study was conducted using 14 albino male rats. The samples were divided into two groups; the control groups (P0) that received aquadest and moderate physical activity, and treatment group (P1) that received 0.4 mg Creatine Monohydrate® supplement twice a day and moderate physical activity given. Moderate physical activity (swimming) was given five times a week for 14 days. Estrogen and testosterone level were assessed before and after treatment was given. Results: Before the treatment, the estrogen and testosterone levels between P0 group and P1 group were comparable. Post-test results showed a significant different in estrogen levels between the P0 and P1 group (23.17±2.86 and 27.82±3.06 ng/ml respectively; p<0.05) as well as testosterone levels (18.15±2.95 and 21.69±2.14 ng/ml respectively; p<0.05) after 14 days of treatment. A paired analysis showed an elevating of estrogen levels but no effect on testosterone levels in P1 group that treated with Creatine Monohydrate® Conclusion: This study indicated that the administration of Creatine Monohydrate® increased the estrogen levels but have no effect on testosterone levels in male albino rats (Rattus norvegicus) with moderate physical activity.

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