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IJAAM (Indonesian Journal of Anti-Aging Medicine)
Published by Universitas Udayana
ISSN : 26147882     EISSN : 26155079     DOI : https://doi.org/10.36675/ijaam
Core Subject : Health,
Medicine & Pharmacology
Indonesian Journal of Anti-Aging Medicine is published by Faculty of Medicine Universitas Udayana in collaboration with Indonesian Center for Anti-Aging Medicine (INCAAM). Indonesian Journal of Anti-Aging Medicine is an open access, peer reviewed journal aiming to communicate high quality research articles, reviews and general articles in the field of anti aging. Indonesian Journal of Anti-Aging Medicine encompass all aspects of basic research/clinical studies related to the field of anti aging and allied science fileds. The Journal aims to bridge and integrate the intellectual, methodological, and substantive diversity of medical scholarship, and to encourage a vigorous dialogue between medical scholars and practitioners. The Journal welcomes contributions which promote the exchange of ideas and rational discourse between practicing educators and medical researchers all over the world.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 73 Documents
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Ashitaba (Angelica keiskei) Leaves Extract Cream 8% Prevented Tyrosinase and Melanin Elevation in the Ultraviolet B-Exposed Guinea Pig (Cavia porcellus) Skin Erwin Sugito; Wimpie Pangkahila; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.99

Abstract

Introduction: Ultraviolet B radiation causes free radical formation, which then activates tyrosinase, an enzyme that plays a role in melanin biosynthesis. The purpose of this study was to prove that Ashitaba (Angelica keiskei) leaves extract cream 8% prevented the increase of tyrosinase levels and the amount of melanin in the ultraviolet B-exposed guinea pig (Cavia porcellus) skin. Methods: This study was conducted with a randomized posttest only control group design. The subjects were guinea pigs (Cavia porcellus), male, healthy, local strains, aged 3-4 months, weighing 300-350 grams, one hybrid, which were divided into 2 groups (n= 18). The control group (ultraviolet B exposure and placebo of basic cream), and the treatment group ultraviolet B exposure and Ashitaba leaves extract cream 8%). After 2 weeks of treatment, tyrosinase levels were examined by the ELISA method, while the amount of melanin was examined using Masson-Fontana staining. Results: The tyrosinase level in the control group was higher than the treatment group. The tyrosinase level of control group was 30.95 ± 3.43 ng/ml while in the treatment group was 11.60 ± 0.34 ng / ml (p <0.05). Similarly, the melanin in the control group was higher than the treatment group. The average number of melanin in the control group was 20.54 ± 2.59% while in the treatment group was 1.96 ± 1.25% (p <0.05). Conclusion: Ashitaba (Angelica keiskei) leaves extract cream 8% prevented the increase of tyrosinase levels and the amount of melanin in the ultraviolet B-exposed guinea pig (Cavia porcellus) skin.
Implantation of Polycaprolactone (PCL) Thread Increased Dermal Collagen of Old Female Wistar Rats (Rattus Norvegicus) More Than Polydioxanone (PDO) Thread Junivan Lindra; Wimpie Pangkahila; Anak Agung Gde Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.77

Abstract

Introduction: Wrinkles and sagging are the sign of skin aging. Threadlift using polydioxanone (PDO) and polycaprolactone (PCL) can increase the amount of collagen. This study aimed to study which thread is better to induced collagenesis. Methods: This study used a posttest-only control group design. The subjects were 30 Wistar rats, healthy, female, age 16-18 months (human equivalent age 45 years), the body weight of 160-180 grams which were divided into three groups (n = 10). The negative control group (P0) was not given any treatment, the positive control group (P1) was treated with 27G needle pierced, 60 mm with 3 PDO threads horizontally in the dermal layer, treatment group (P2) was treated with 27G needle pierced, 60 mm with 3 PCL threads horizontally in the dermal layer. After 4 weeks, a 5x5mm biopsy was performed on the skin and histopathological preparations were made with Sirius Red staining. Results: The results showed that the amount of collagen in the P0 group was 75.0±1.51, in the P1 group was 82.3 ± 1.49, while in the P2 group was 86.0 ± 1.22 (p <0.001). The LSD test showed that there were differences between the P1 group and the P2 group (p <0.001), where the amount of dermal collagen in the PCL group was higher compared to the PDO group. Conclusion: It can be concluded that the implantation of polycaprolactone (PCL) thread increased dermal collagen of old female Wistar rats (Rattus norvegicus) more than polydioxanone (PDO) thread.
The akar kucing (Acalypha indica l.) Leaves extract cream 4% prevented the formed melanin cell of guinea pigs (Cavia porcellus) skin that exposed to ultraviolet B Aditia Martanti; Wayan Weta; Aagp Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.62

Abstract

Background: Hyperpigmentation is caused by an increase in the amount of melanin in the epidermal skin layer. The natural topical ingredients possibly has a role protect and reduce damaged caused by exposure to ultraviolet rays, with minimal side effects. The active compounds in akar kucing leaves are flavonoids, tannins and phenols. The aim of the study is to prove the administration of akar kucing (Acalypha indica L.) leaves extract 4% can prevented the formed of melanin cell in guinea pig skin (Cavia porcellus) that exposed to UVB. Methods: The randomized post-test only control group design, was conducted with 36 male guinea pigs (Cavia porcellus), local strains, aged 3 months, weighing 300-350mg. Both group was exposed to UVB for 5 minutes with dose 65 mJ/cm2, six times for 2 weeks. The control group was given basic cream. The treatment group was given akar kucing (Acalypha indica L.) leaves extract 4% cream. After two weeks intervention, punch biopsied tissue of guinea skin performed to subcutaneous then it was stained with Masson-Fontana. Finally melanin cell was counted compared to epidermic area in percentage unit. Results: All samples of guinea pigs were successful until they were fit for post-test. The melanin cell in the treatment group with 1.65 ± 0.495% was lower than melanin cell in the control group 18.5 ± 1.18, significantly (p <0,001). Conclusion: Cream of akar kucing (Acalypha indica L.) leaves extract 4% prevented the formed of melanin cell of the skin of guinea pig (Cavia porcellus) that exposed to UVB.
Administration of Acacia (Acacia mangium) Leaves Ethanol Extract Cream 5% Prevented The Increase of Melanin Number in Ultraviolet B-Exposed Guinea Pig (Cavia Porcellus) Skin Olivia Julita; Ida Sri Iswari; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.101

Abstract

Introduction: Ultraviolet exposure stimulate oxidative stress-mediated melanogenesis, which is the sign of photoaged skin. Acacia (Acacia mangium) leaves extract contains high levels of antioxidants. The aim of this study was to prove the effectiveness of the Acacia leaves ethanol extract cream in preventing an increase of melanin. Methods: This experimental study used randomized posttest only control group design. The research subjects were 36 male guinea pigs (Cavia porcellus), healthy, local strains, aged 3 months, weighing 300-350 grams and had never received prior treatment. Subjects were divided into 2 groups (18 individuals each), namely the control group (treatment with ultraviolet B exposure and basic cream) and the treatment group (treatment with ultraviolet B exposure and acacia leaves ethanol extract cream 5%). After 2 weeks of treatment, the amount of melanin was examined histopathologically using Masson-Fontana staining. Results: The average number of melanin in the control group was 19.33±2.59% while in the treatment group was 2.21±1.25%. Comparative analysis using independent t-test showed that the value of p<0.001. Conclusion: This study suggesting that administration of acacia (Acacia mangium) leaves ethanol extract cream 5% prevented increasing amount of melanin in ultraviolet B-exposed guinea pig (Cavia porcellus) skin. The results showed that there was number of melanin in the control group (19.33±2.59%) were higher than the treatment group (2.21±1.25%) (P<0.001).
The 20% of petai seed (Parkia speciosa) extract cream in inhibited the increase of melanin counts in the skin of guinea pigs (Cavia porcellus) exposed to ultraviolet B Nining Triyana Darise; Wimpie Pangkahila; AAGP Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.87

Abstract

Introduction: The exposure to UV may cause skin disorders, including hyperpigmentation. Petai seeds are categorized as traditional medicinal plants that have anti-inflammatory and antioxidant effects, such as flavonoids, tannins, phenols and vitamin C which have the function of inhibiting the tyrosinase enzyme and the synthesis of melanin. The aim of this research was to prove that a 20% of Petai seed (Parkia speciosa) extract cream could prevent the increase of melanin counts in the skin of Guinea Pigs (Cavia porcellus) exposed to UV-B. Method: This research employed an experimental study through a randomized post-test only control group design that used 36 male guinea pigs (Cavia porcellus) that divided into 2 (two) groups each of which were 18 guinea pigs. In order to find out the mass, melanin stained with Masson-Fontana, the melanin count was showed in percent. The results of this research were then analyzed and used descriptive analysis, data normality, data homogeneity, and comparability tests. Results: The results indicated that the mean was 20.543 ± 3.344%, whereas, in the treatment group that smeared by 20% of petai seed extract cream, the mean value was 1.533 ± 0.518% (p <0.001). This indicated that the treatment group had a smaller melanin count than the control group. Conclusion: Based on the results of this research, it can be concluded that 20% of petai seed extract cream inhibited the increase of melanin counts in guinea pigs’ skin exposed to UV-B.
Topical administration of 40% soursop (Annona muricata) leaves extract cream prevented melanin elevation in UVB-rays exposed guinea pigs (Cavia porcellus) skin Maya Cesarya Simangunsong; Wimpie Pangkahila; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.73

Abstract

Introduction: Chronic exposure to UV light can cause skin abnormalities such as hyperpigmentation due to increased amount of melanin. Soursop (Annona muricata) leaf is a plant that contain flavonoids, polyphenols and antioxidants so that they have the potential as anti- hyperpigmentation. The purpose of this study was to prove that topical administration of 40% soursop leaf extract cream prevents melanin elevation in UVB-rays exposed guinea pigs skin. Methods: This study was an experimental study using randomized posttest only control group design. Subjects were 36 male guinea pigs (Cavia porcellus), brown, adult (3 months old), weighing 300-350 grams which were divided into two groups (n= 18). One group as the negative control group that was exposed to UVB and treated with basic cream (P0), and another group as the treatment group that was exposed to UVB and treated with 40% soursop (Annona muricata) leaves extract cream. The amount of melanin was measured histopathologically using immunohistochemical Masson-Fontana staining. Results: The results showed that the average amount of melanin in control group was 13.756 ± 5.2120, whereas in the 40% soursop (Annona muricata) treatment group was 6.811 ± 2.8105. Comparative analysis using independent t-test showed the p value of <0.001, thus indicating a significant difference between groups after treatment. Conclusion: Based on the results of this study it can be concluded that topical administration of 40% soursop (Annona muricata) leaves extract cream prevented melanin elevation in UVB- rays exposed guinea pigs (Cavia porcellus) skin.
Administration of White Turmeric (Curcuma zedoaria) Extract Increased Glutathion Peroxidase Levels and Reduced Malondialdehyde Mice (Mus musculus) Male Strain Balb/c That Were Exposed by Vehicle Fumes Ana Yulia; I Wayan Weta; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.58

Abstract

Introduction: Currently, air polution is a global health problem, including in Indonesia as developing country. Air pollution is known as oxidants and induces cell damage through the free radicals formation. In order to prevent oxidative stress due to exposure to air pollution, administration of exogenous antioxidants is necessary. White turmeric (Curcuma zedoaria) contains curcumin, tannin and polyphenols antioxidant activity may work to neutralize the effect that caused by air pollution. The aim of this study was to prove that supplementation of white turmeric extract increased glutathione peroxidase and decreased malondialdehyde in mice male strain Balb/c that were exposed by vehicles fumes. Methods: The randomized pretest-posttest control group design was conducted. The subjects 10 mice (strain Balb/c), male, age 2-3 months (adult mice), weight 20-22 grams, and healthy. Mice were divided into two groups (five mice each), namely the control and treatment group. Control group was given placebo of 2 ml aquabidest for 16 days and the treatment group was supplemented with white turmeric extract of 100 mg/kg BW for 16 days while exposed by motor vehicle fumes for those periode of time. Before and after treatment, Glutathion Peroxidase (GPx) and Malondialdehyde (MDA ) were measured using ELISA method. Results: Unpaired data comparison showed before the treatment (pretest), there were no differences in GPx levels (control 8.72±0.094, treatment 8,81±0,063, p= 0.114) and MDA levels (control 4,19±1,55, treatment 4,43±1,49, p= 0.812) between groups. While after treatment (posttest), the mean GPx levels (control 8.76±0.075, treatment 12.0±0.296; p <0.001) and MDA levels (control 2,.71±0.409, treatment 1.56±0.0019, p= 0.001) differed significantly Paired data analysis showed that in the control group there was a non-significant increase in GPx level (from 8.72±0.04 U to 8.76±0075 U; p=0.545), while in treatment group there was a significant increase in GPx levels (from 8.81 ± 0.063 U to 12.0 ± 0.296 U; p <0.001). In the control group there was a non-significant reduction in MDA level (from 4.19±1.55 U to 2,71±0.409 U; p=0.161, while in treatment group there was a significant reduction in MDA levels (from 4.43 ± 1.49 µM to 1.56 ± 0.326 µM; p= 0.019). Conclusion: Administration of white turmeric (Curcuma zedoaria) extract increased GPx levels and reduced mMDA in mice (Mus musculus) male strain Balb/c that were exposed by vehicles fumes.
The oral administration of zinc sulfate in increasing the mass of pancreatic β cell and reducing the level of fasting blood glucose and glycated albumin in male wistar rats (Rattus norvegicus) with diabetes mellitus Silvia Irani; Anak Agung Gede Budhiarta; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.82

Abstract

Introduction: Diabetes mellitus (DM) is a degenerative disease that can accelerate the aging process. Zinc is a mineral that has antioxidant potential that can increase the cell growth and it also protects pancreatic β cell mass from cell death during diabetes. This study aimed to prove that oral administration of zinc sulfate could increase the pancreatic β cells mass and decrease the level of fasting blood glucose (FBG) and glycated albumin (GA) in male Wistar rats with DM. Methods: This research employed a pure experimental research with post test only control group design by using 36 male Wistar rats. The placebo group that administered with 1 cc of aquadest and the group of zinc that administered by oral zinc sulfate of 100 mg/kgBW in which had been dissolved with 1 cc of aquadest. The assessment of pancreatic β cell mass and FBG levels and glycated albumin were carried out after 30 days of treatment. Results: The analysis by using the Shapiro-Wilk test was distributed normally. The result of Laven’s test indicated that the distribution of data and variants of the groups were homogeneous. The comparative results of the two groups using an independent t-test, there were significant differences between both groups on the mass of pancreatic β cell and the level of fasting blood glucose and glycated albumin (p <0.05). Conclusion: The oral administration of zinc sulfate can increase the pancreatic β cell mass and decrease the level of FBG and glycated albumin in male Wistar rats with DM.
Administration of oral white oyster mushroom (Pleurotus ostreatus) ethanol extract improved lipid profile as effective as simvastatin in dyslipidemic male wistar rats (Rattus norvegicus) Nadia Anastasia; I Wayan Weta; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.93

Abstract

Introduction: Dyslipidemia is an abnormality in lipid metabolism characterized by an increase in total cholesterol, LDL, triglyceride, and decrease HDL. Simvastatin is currently a gold standard drug that has side effects, so an alternative therapy that is relatively safe is needed. The white oyster mushroom extract contains bioactive compounds that have the anti-dyslipidemia effect. The purpose of this study was to prove that the administration of oral white oyster mushroom (Pleurotus ostreatus) extract improves lipid profile as effective as simvastatin in dyslipidemic male Wistar rats (Rattus norvegicus). Methods: This study was an experimental study with pretest-posttest control group design by using 12 rats. Rats were induced by dyslipidemic with a high cholesterol diet for 28 days and divided into 2 groups. The control group was fed with standard food and statins of 10 mg/day and the treatment group was fed with a standard diet and white oyster mushroom extract. Results: The results of paired data analysis showed that both simvastatin and white oyster mushroom extract reduced total cholesterol (p<0,001) and (p<0,001), triglycerides (p<0,001) and (p=0,005), LDL (p<0,001) and (p<0,001) and increased HDL (p <0,001) and (p<0,001). Between groups before treatment (pretest) showed no differences levels in total cholesterol, triglyceride, LDL, and HDL. After treatment (posttest), there were also no differences levels between groups in total cholesterol (p=0,097), triglyceride (p=0,160), LDL (p=0,980), and HDL (p-0,452). Conclusion: The administration of oral white oyster mushroom (Pleurotus ostreatus) extract improved lipid profile as effective as simvastatin in dyslipidemic male Wistar rats (Rattus norvegicus).
Implantation of Polydioxonone (PDO) Threads Decreased Dorsolumbar Subcutaneous Fat Weight in Obese Female Rats (Rattus Norvegicus) Diana Niti Santoso; Anak Agung Gde Putra Wiraguna; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.78

Abstract

Introduction: Thread-lifts, especially using polydioxanone (PDO), are thought to have targeted lipolysis effect, but have never been empirically proven. The aim of this study was to prove that the implantation of polydioxonone (PDO) threads decrease dorsolumbar subcutaneous fat weight in obese female rats (Rattus norvegicus). Methods: This experimental study used posttest only control group design. The research subjects were 36 healthy Wistar rats, females, aged 16-18 weeks, obese with a body weight of 250-300 grams (Lee index criteria> 0.3). Obesity was induced by high-carbohydrate and high-fat feed. The rats were then divided into 2 groups, the negative control group with catgut thread implant treatment (P0) and the treatment group with polydioxonone (PDO) thread implant treatment (P1). After 28 days the rats were euthanized, skin tissue was collected, subcutaneous fat was searched and separated from the dorsolumbar depots found in the subcutaneous layer in the lumbar spine at the dorsal part of the body, then measured with the Sartorius brand scales. Results: The median weight of dorsolumbar subcutaneous fat in the P0 group was 1.05 (0.80; 1.90) grams, while in P1 group was 0.90 (0.70; 1.10) grams. The results of the comparative analysis with Mann Whitney test showed a p value of 0.016, which means that there was a difference in dorsolumbar subcutaneous fat between groups after 28 days. Conclusion: Implantation of polydioxonone (PDO) threads decreased dorsolumbar subcutaneous fat weight in obese female rats (Rattus norvegicus).

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