cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Sagung Chandra Yowani
Contact Email
jhsm@unud.ac.id
Phone
-
Journal Mail Official
jhsm@unud.ac.id
Editorial Address
Institute for Research and Community Services Udayana University Gedung LPPM lantai 4 Bukit Jimbaran, Badung, Bali, Indonesia-80361
Location
Kota denpasar,
Bali
INDONESIA
Journal of Health Sciences and Medicine
Published by Universitas Udayana
ISSN : 25497561     EISSN : 26220555     DOI : -
Core Subject : Health,
Medicine & Pharmacology
Journal of Health Sciences and Medicine is an Indexed peer-reviewed open access journal which publishes original scientific articles, reviews and short communications that are not under consideration for publication elsewhere. The journal publishes papers based on original research that are judged by critical reviews, to make a substantial contribution in the field. It aims at rapid publication of high quality research results while maintaining rigorous review process. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers are published approximately one month after acceptance.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 25 Documents
 1   2   3 
Rapid Detection Of Methicillin Resistant Staphylococci Using Multiplex PCR With Boiling Method For DNA Isolation Ida Bagus Gede Adiguna Wibawa; Agus Eka Darwinata; Ni Nengah Dwi Fatmawati; Nyoman Sri Budayanti
Journal of Health Sciences and Medicine Vol 1 No 2 (2017): JHSM (September 2017)
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.626 KB) | DOI: 10.24843/JHSM.2017.v01.i02.p03

Abstract

Methicillin-Resistant Staphylococcus aureus (MRSA) is Staphylococcus aureus that has become insusceptible or resistant by methicillin antibiotic types. Rapid identification of MRSA is essential for early initiation of appropriate antimicrobial therapy. The aim of this research is to reduce the cost and time needed for multiplex PCR in rapid detection of MRSA by finding an alternative method for DNA which is boiling method. DNA isolation was performed with boiling method and kit commercial. The kit method takes time approximately 45 minutes while boiling takes only about 12 minutes. PCR result with boiling technique used in DNA isolation formed amplification bands of 16S rRNA, mecA , and nuc in MRSA and 16S rRNA and nuc in MSSA. Conclusion can be drawn that boiling method can be used as an alternative method for DNA extraction.
EFFECTIVENESS OF LEAF EXTRACT REDUCE INFLAMMATION REACTION IN HYPERCHOLESTEROLEMIA RATS Puguh Santoso; Ni Nyoman Wahyu Udayani; I Nyoman Gede Tri Sutrisna; Ketut Agus Adrianta
Journal of Health Sciences and Medicine Vol 1 No 1 (2017): JHSM (Febuary 2017)
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.677 KB) | DOI: 10.24843/JHSM.2017.v01.i01.p04

Abstract

Abstract High blood cholesterol is often called hypercholesterolemia is a risk factor for the emergence of pathological conditions such as heart and blood vessel disease. Hypercholesterolemia has an important role in the occurrence of damage to the endothelial cells is mainly caused by oxidized LDL. Oxidation of LDL triggers the formation of TNF - ?. Leaves messengers that allegedly contains flavonoids can improve the situation of hypercholesterolemia through the barriers specifically the expression of TNF - ? increased due to hypercholesterolemia. Plants messengers known to contain alkaloids, flavonoids, tannins, saponins, polyphenols, calcium oxalate, fats, and essential oil. Flavonoids which has the ability to bind the atom to form free radicals not to excess free radicals, thereby inhibiting the oxidative modification of LDL become ox-LDL so it will not be formed atherosclerosis. This study uses the Randomize pattern Pre and Post Test Control Group Design, using white rats (Rattusnovergicus) with Wistar strain aged 3-4 months, weighing 175-200 grams. Divided into four groups: Group I as a control with placebo, Group II treatment using extracts of ethanol leaves a messenger at a concentration of 10%, Group III treatments using extracts of ethanol leaves a messenger at a concentration of 20%, Group IV is to use the extract ethanol leaves errand at a concentration of 30%. It can be concluded at 4 dose group 30% messengers leaf extract significantly different, p <0.05, so it can be said that the provision of effective messengers extract at a dose of 30%.
DNA Probe Design for Detection Mutation at Codon 315 In katG Gene of Mycobacterium Tuberculosis to Real-Time Polymerase Chain Reaction I Gusti A. A. Santhi Rahmaryani; Ni Kadek Ariani; Dyah Subadrika Warma Dewi; Ni Komang Sasi Ani; Ade Ari Sundari; Kadek Widya Yuli Hartati; Sagung Chandra Yowani
Journal of Health Sciences and Medicine Vol 1 No 2 (2017): JHSM (September 2017)
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (773.559 KB) | DOI: 10.24843/JHSM.2017.v01.i02.p08

Abstract

High-level resistance to isoniazid as a first-line tuberculosis drugs can be caused by mutations in codon 315 katG Mycobacterium tuberculosis . Mutation at codon 315 is the most frequent mutation with the highest amino acid variation, compared to other codons in the Mycobacterium tuberculosis katG gene. Therefore, a specific probe is required for rapid and proper detection of mutations at codon 315. In this study, the design of a nucleotide sequence probe with TaqMan labeling was performed using Clone Manager Suite 6 software . The mutant probe obtained was analysed in two stages. The initial analysis is based on the length of the probe (22-30 bases), Tm (70ºC), %GC (35-65%), not in hairpin form, dimer (< 5 bases), runs and repeat (? 4 for base A, T, C, and < 3 for base G). Furthermore the final analysis was carried out with no G base in 2 bases at the end of the 5’ probe and the amount of base C ? G. The study resulted in 260 probe mutants. After the initial analysis, 11 mutant probes were obtained to recognize mutations in the codon of 315 katG Mycobacterioum tuberculosis genes. The probe consists of 2 probes for the S315T mutation, 6 probes for S315N mutation, and 3 probes for S315V mutation. The criteria of the 11 mutant probes are 22-23 bases long, Tm 70ºC, % GC 56-63%, 4 dimer , 2 runs , and does not have repeats and does not form hairpin at a temperature of 56ºC. Based on the final analysis, 3 mutant probes were obtained fulfilling the TaqMan probe labeling criteria, namely K315MT1 for specific detection of mutation S?T and K315MN5, then K315MN23 for specific detection of mutation S?N. The conclusion of this study shows that the best mutant nucleotide sequence probes for the detection of mutations at codon of 315 KatG Mycobacterium tuberculosis genes are 5’-FAM-CC ACC GGC ATC GAG GTC GTA TG-TAMRA-3’; 5’FAM-ATC ACC AAC GGC ATC GAG GTC G-TAMRA-3’; dan 5’FAM-C ACC AAC GGC ATC GAG GTC GTA T-TAMRA-3’. The design of the mutant probe according to the TaqMan probe criterion for real-time PCR was obtained by 3 probes from the 11 selected mutant probes in the initial analysis.
P-Glycoprotein Expression on Patients with Acute Lymphoblastic Leukemia R. Niiruri; I. Narayani; K. Ariawati; S. Herawati
Journal of Health Sciences and Medicine Vol 1 No 1 (2017): JHSM (Febuary 2017)
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (95.865 KB) | DOI: 10.24843/JHSM.2017.v01.i01.p10

Abstract

Abstract Objective: P-glycoprotein (P-gp) overexpression on neoplastic cells can deteriorate the therapeutic outcome on cancer patients. P-gp plays important role on drug efficacy and toxicity. This research aimed to measure P-gp expression on children with Acute Lymphoblastic Leukemia (ALL) on Sanglah Hospital, Denpasar. Method: Flowcytometry method was used to measure P-gp expression level on Bone Marrow samples from pediatric patients (0-12 years old) who were newly diagnosed with ALL in Sanglah Hospital. P-gp overexpression were based on the percentage of cell stained. Ten percent of P-gp expression were considered as the cut-off value of P-gp overexpression. Result: On this study, 11 samples were obtained with the range value of 56-97% on P-gp expression. Conclusion: All 11 patients had P-gp overexpression.
DIFFERENCE OF SGOT AND SGPT LEVEL IN STADIUM IIB-IIIB SQUAMOUS CELL CERVICAL CANCER PATIENTS BEFORE AND AFTER CHEMOTHERAPY AT SANGLAH HOSPITAL DENPASAR R. Noviyani
Journal of Health Sciences and Medicine Vol 2 No 1 (2018): JHSM (February 2018)
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.482 KB)

Abstract

Objective:Cervical cancer became Indonesia’s highest prevalent gynecological cancer in 2013 and the highest prevalent gynecological cancer at Sanglah Hospital.Paclitaxel Carboplatin is one of the regimens used for cervical cancer treatment at Sanglah Hospital, Denpasar. Aside of providing therapeutic effect, this regimen also causes hepatotoxicity. This research was trying to determine the toxic effect of Paclitaxel Carboplatin towards liver function based on the difference of SGOT and SGPT levels before chemotherapy cycle I and after chemotherapy cycle VI. Method:This wasa prospective observational research with a study case method conducted from January 2017 until June 2017 at Obstetric Polyclinic of Sanglah Hospital, Denpasar.Samples’ SGOT and SGPT level before and after chemotherapy I and VI were recorded and were then analysed with Shapiro-Wilk normality test. If the data were distributed normally, they would undergo tpaired test and Wilcoxon test at 95% confidence level if they were not distributed normally. Results: Tenpatients fulfilled the research criteria. There was a meaningless increase for SGOT level (p=0.575) along with a meaningless increase for SGPT level (p=0.074) before and after Paclitaxel Carboplatin chemotherapy cycle I and VI respectively.Conclusion: Research of toxic effect from Paclitaxel Carboplatin chemotherapy in 10 squamous cell cervical cancer patients showed a meaningless difference of both SGOT and SGPT level with the value of p>0.05 in stadium IIB-IIIB squamous cell cervical cancer patients before and after Paclitaxel Carboplatin chemotherapy cycle I and VI respectively.

Page 3 of 3 | Total Record : 25

 1   2   3 

Filter by Year

2017 2018


Reset
Filter By Issues
All Issue Vol 2 No 1 (2018): JHSM (February 2018) Vol 1 No 2 (2017): JHSM (September 2017) Vol 1 No 1 (2017): JHSM (Febuary 2017)