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Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Published by Universitas Nusa Nipa
ISSN : 2716151X     EISSN : 2722869X     DOI : http://dx.doi.org/10.55241/spibio
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Other
Jurnal ini fokus pada bidang kajian biologi sains dan pendidikan biologi. Kami menerima artikel berupa hasil penelitian maupun kajian pustaka yang original dan belum pernah dipublikasi.
Arjuna Subject : Umum - Umum
Articles 392 Documents
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Pemberian Pupuk Kompos dan Cair Azolla terhadap Pertumbuhan dan Kandungan Flavonoid Tanaman Kedelai Hitam (Glycine max L.) Widia Hariani; Rahmadina Rahmadina; Leni Widiarti
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.545

Abstract

Black soybean (Glycine max L.) is a food crop commodity. The low productivity of soybean in Indonesia is caused by several factors, including the availability of low-quality seeds and the unavailability of nutrients. Therefore, this study aims to determine the effect of compost and liquid Azolla fertilizer (Azolla pinnata) on the growth and flavonoid content of black soybean plants (Glycine max L.). With the factorial RAK method. The first factor is without Azolla compost fertilizer (K0), Azolla compost fertilizer 250 gr / polybag (K1), Azolla compost fertilizer 300 gr / polybag (K2). The second factor is without liquid azolla fertilizer (C0), azolla liquid fertilizer 130 ml / l of water (C1), azolla liquid fertilizer 150 ml / l of water (C2). And the data is processed using SPSS with the ANOVA test. If the treatment obtained has a very significant effect, it will be continued with the DMRT test. The results showed that Azolla compost fertilizer affected plant height, number of leaves and total leaf area with a dose of 250 gr/polybag. While Azolla liquid fertilizer did not affect all parameters at all doses, namely 130 and 150 gr/polybag. The highest flavonoid content in black soybean leaves was found in the 250gr compost fertilizer treatment, namely 99.72247 QE/g. It can be concluded that Azolla compost fertilizer is more effective in increasing the flavonoid content of black soybean leaves. The flavonoid content of the 250gr compost fertilizer treatment was 99.72247 QE/g in the leaves.
Analisis Kekerabatan Menggunakan Metode Fingerprint DNA pada Ikan Sepat (Ordo Anabantiformes) di Perairan Kecamatan Kualuh Leidong Desi Ritonga; Zahratul Idami; Kartika Manulu
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.550

Abstract

Gourami is a type of freshwater fish that is widely distributed in Indonesia, including in the waters of Kualuh Leidong District, North Sumatra. However, the kinship relationship between gourami species in the region has not been widely studied. Therefore, this study aims to analyze the kinship relationship of several gourami species found in the waters of Kualuh Leidong using the DNA fingerprint method. This study uses a molecular characterization method with several stages, namely collecting fish samples, isolating and extracting DNA, amplifying DNA using Polymerase Chain Reaction (PCR) with several primers, electrophoresis, and analyzing data using NTSYS software. Sampling was carried out by collecting specimens from the waters of Kualuh Leidong, including four types of gouramis, namely Trichopodus trichopterus, Trichopodus pectoralis, and Helostoma temmincki. The electrophoresis results showed that the DNA of the gourami fish could be amplified with primers OPA 02, OPC 02, and UBC 456, but not amplified with primer OPA 20. The DNA banding pattern obtained consisted of polymorphic and monomorphic bands with varying locus sizes. Jaccard coefficient analysis showed a high level of kinship (>70%), with the species Trichopodus pectoralis having a similarity of 0.78, Trichopodus trichopterus 0.76, and Helostoma temmincki 0.71. The results of this study can be used as a basis for the conservation and management of gourami fish resources in the waters of North Sumatera.
Analisis Konsentrasi CO2 di Kawasan Pasar Wisata Bojonegoro Evi Cici Kumala; Heri Mulyanti; Solikhati Indah Purwaningrum
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.553

Abstract

Carbondioxide (CO?) is one of the greenhouse gases that contribute to climate change and global warming. Indoor and urban CO? concentrations can affect air quality and human health. Pasar Wisata Bojonegoro is a central economic hub with high mobility and trading activities, which may increase CO? emissions. This study aims to identify peak CO? concentration hours in the area and compare them with the air quality standards set by the WHO. A quantitative research method was used, involving direct observation and measurement over 7 (seven) days at a single observation point. The results show that on weekdays, the peak CO? concentration occurred between 16:00 and 16:59 WIB, reaching 424 ppm, while the lowest concentration was recorded between 18:00 and 18:59 WIB at 351 ppm. On weekends, the peak concentration was 441 ppm between 16:00 and 16:59 WIB, while the lowest was 113 ppm between 21:00 and 21:59 WIB. Several weekdays recorded CO? concentrations exceeding the WHO standard (310–330 ppm), with the highest reaching 466 ppm. However, CO? concentrations on Thursdays and weekends remained within the acceptable limits. The conclusion of this study shows that the results of CO? concentration on weekdays (Monday, Tuesday, Wednesday, Friday) exceed the quality standard with CO? concentrations of 466 ppm, 462 ppm, 413 ppm, and 433 ppm. Meanwhile, on weekdays (Thursdays) and weekends, it meets the quality standards with CO? concentrations of 217 ppm, 243 ppm, and 267 ppm.
Pengaruh Pemberian Rendaman Kulit Bawang Merah (Allium cepa L.) dengan Media Tanam Ampas Tebu terhadap Pertumbuhan Tanaman Pakcoy (Brassica rapa L.) Nuri Jannah Tussadiyah Siregar; M Idris; Irda Nila Selvia
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.554

Abstract

Pakcoy comes from the Brassicaceae family, known for being rich in nutrients, including vitamins A, C, K, as well as fibres and minerals. Onion skin contains bioactive compounds that have the potential to be organic fertilizers, while sugarcane bagasse is agricultural waste that can be used as an alternative planting medium. This study aims to determine the effect of soaking shallot skin using sugarcane bagasse planting media for the growth of Pakcoy (Brassica rapa L.). This study was conducted in Tuntungan II village using the factorial Randomized Block Design (RAK) method consisting of two factors and 3 replications with 3 treatment levels. The parameters observed included relative growth rate, net assimilation rate, number of leaves, total leaf area, wet weight and dry weight of pakcoy plants. The results showed that the combination of onion skin soaking gave a significant effect on all red parameters (B2: 250 ml/500 ml water) and bagasse gave a significant effect on the net assimilation rate of 2-3 MST and 4-5 MST in the treatment of 500 gr/polybag bagasse but on other parameters the control treatment was better. Thus, the provision of onion skin soaking as organic fertilizer with bagasse planting media on the growth of pakcoy plants can be applied in agriculture, because this treatment has a significant effect on all treatments.
Penurunan Total Coliform Mata Air dengan Desain Biofilter Limbah Organik Viennye Wilhelmina Djawa; Gracyana Putri Sarifan; Maria Astin Rosari Tahu; Chatarina Gradict Semiun
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.555

Abstract

Unconserved springs tend to be polluted by various organic wastes. Accumulation of organic waste causes water to be contaminated with coliform bacteria, thus reducing water quality. Biofilter technology can be applied to reduce the number of coliform bacteria. This study aimed to determine the ability of BioFilter K4Ser-Col (moringa seeds, rice husks and kesambi charcoal) to reduce total coliform in spring water. The research location was at the Riumata Spring, Nekbaun village, West Amarasi sub-district, Kupang district. The work consisted of preparing the BioFilter K4Ser-Col media, taking water samples, and testing MPN coliform. MPN coliform testing was done twice before and after the water filtration on the BioFilter K4Ser-Col media. The study's results showed a decrease in the MPN value before and after filtering. The MPN coliform value before filtering was 1600 MPN/100 mL. After filtered water on the BioFilter K4Ser-Col media, it decreased to 23.23 MPN/100 mL. This value had met the national water quality standards for the class I category based on PP No. 22 of 2021. Thus, the use of BioFilter K4Ser-Col media could greatly reduce the number of coliform bacteria in water.
Analisis Peran Transpor Membran dalam Respons Stres Abiotik: Implikasi pada Pertumbuhan dan Perkembangan Tanaman Dian Dwi Lestari; Nia Febrianti; Adnan
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.556

Abstract

Abiotic stress is an environmental condition that is unfavorable for plants such as drought, salinity, heavy metal contamination and nutrient deficiencies. Prolonged abiotic stress causes the stability of plant cell organelles to be disrupted, thereby inhibiting plant growth and development. This research aims to examine the role of membrane transport in responding to environmental stress, especially abiotic stress, and the implications for plant growth and development. The research method used was a literature study using meta-analysis techniques assisted by prism diagrams in analyzing 14 relevant scientific articles from various national and international journals. The analysis was carried out descriptively on the results of previous research. The results show membrane transport has a role in increasing plant resistance to abiotic stress. Cell membrane structure consisting of lipids, proteins and carbohydrates has been proven to increase plant safety from environmental stress. So it can be concluded that membrane transport has a role in mediating tolerance, resistance, and resolving plant stress conditions through maintaining cellular homeostasis. Implications of this research can help expand knowledge about how plants respond to unfavorable environmental conditions through membrane transport mechanisms and can be basis for developing plant varieties are resistant to various forms of environmental abiotic stress.
Pemanfaatan Limbah Sekam Padi (Oryza sativa Linn.) sebagai Media Fermentasi untuk Produksi Enzim Selulase oleh Aspergillus niger Eksa Adhwa Fadhilah; Peristiwati; Wahyu Surakusumah
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.559

Abstract

This study aims to optimize the production of cellulase enzyme by the cellulolytic fungus Aspergillus niger using rice husk powder (Oryza sativa Linn.) as substrate. Cellulase enzymes have important roles in various industrial applications, including the bioconversion of lignocellulosic waste into high-value products such as bioethanol, pulp, paper, and animal feed. The utilization of rice husk as a substrate offers economic and environmental advantages due to its abundant availability, low cost, and ability to reduce the volume of agricultural waste. The research method used a quantitative experimental approach to evaluate the effect of fermentation parameters, such as pH, temperature, and incubation time, on enzyme production. A. niger isolates were developed on carboxymethylcellulose (CMC) media to ascertain their cellulolytic capacity, with measurements of biomass, pH, temperature, and enzyme activity. The results showed that the biomass of A. niger increased from hour 0 to hour 96 at pH 4.5 to 5.5, and temperature 29.5°C to 30.5°C. The optimum condition for enzyme production was found at pH 4.5 and temperature 30.5°C, with the highest enzyme activity reaching 0.736 U/mL. These findings suggest that optimization of cellulase enzyme production from rice husk not only improves enzyme production efficiency, but also contributes to more sustainable waste management.
Eksplorasi Keanekaragaman Jamur Edible di Pasar Jakarta dan Bekasi: Potensi Pangan Fungsional dan Bioaktivitasnya Ezra Angela; Dian Pirma Natasari Purba; Frisca Olivia Siahaan; Agnes Feronika Situmorang; Anne Serafin Hutabarat; Jendri Mamangkey; Sunarto
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.562

Abstract

Biodiversity, especially edible mushrooms, plays a crucial role in food sustainability and public health. Edible mushrooms, such as Shiitake and oyster mushrooms, are known to be rich in nutrients and bioactive compounds that are beneficial for health, including antioxidant and anticancer effects. This research aims to identify various types of edible mushrooms sold in traditional and modern markets in several areas of Jakarta and Bekasi, as well as to evaluate their potential as sources of functional food and biopharmaceuticals. The exploration method was conducted through direct visits to several markets, where data was collected and analyzed descriptively. The results indicate that there are 10 species of edible mushrooms in the Jakarta area and 8 species in Bekasi, with ear mushrooms and oyster mushrooms being the most commonly found. Public knowledge regarding the health benefits of edible mushrooms is still limited, thus this research is expected to raise awareness about the importance of utilizing edible mushrooms as part of a healthy diet and their potential in the food and health industry. Public awareness related to a healthy diet, edible mushrooms can be used as an attractive alternative in natural medicine and nutrition, supporting food sustainability in Indonesian society.
Analisis Kemampuan Representasi Visual Siswa dalam Menginterpretasi Diagram Siklus Hidup Bryophyta: Analisis Kemampuan Representasi Visual Siswa dalam Menginterpretasi Diagram Siklus Hidup Bryophyta Dewi Susanti; Adi Rahmat
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.564

Abstract

Visual representation is important for scientific communication. One form of visual representation is a diagram. When representing diagrams, there are still students who fail to read labels or titles, misunderstand color codes, or follow arrows in the wrong direction. The aim of this research is to analyze students' ability to visually represent and interpret the Bryophyta life cycle diagram. The visual representations analyzed are internal and external visual representations. This study uses a descriptive approach involving one biology teacher and 33 students from grades X, XI, and XII at one of the high schools in the city of Bandung. Data were obtained through worksheets and semi-structured interviews. Internal visual representation is obtained from the activity of reading and re-representing the available diagrams, while external visual representation is obtained from the activity of redrawing the Bryophyta diagram based on one's own understanding. Assessment is conducted based on three main indicators: the number of phases, the sequence of phases, and the accuracy of phase descriptions. Scores are converted into percentages and categorized into five levels of proficiency: very good, good, moderate, poor, and very poor. The analysis results show that the internal visual representation of students in grades X, XI, and XII falls into the poor category, while the external visual representation of students in grades X and XI falls into the good category, and grade XII falls into the moderate category. The analysis also shows a relationship between visual representation and prior knowledge. These findings have important implications for the development of visual-based learning and mapping students' difficulties in understanding diagrams.
Hubungan Fenetik Pisang (Musa Spp) berdasarkan Karakter Morfologi di Wilayah Provinsi Gorontalo Elsi Gogou; Novri Youla Kandowangko; Febriyanti; Jusna Ahmad; Aisyah Ahmad
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.566

Abstract

Banana (Musa spp) is a tropical fruit plant that has many benefits and important roles for the community. This study aims to provide information on the morphological characteristics of bananas in the Gorontalo Province and their phenetic relationships. This study uses a descriptive method to describe the diversity of morphological characters of bananas (Musa spp) with data collection using a survey study method (exploration) which is carried out by tracing or exploring the location of the Gorontalo Province, purposive sampling (Purposive Stratified Sampling) by seeing and observing directly the morphological characters of bananas (Musa spp). Data analysis using the Unweighted Pair Group with Mean of Arithmetic (UPGMA) method with the Gower Genelar Similarity Coeffiicient formula in the MVSP (Multi-Variate Statistical Package) v.3.1 computer program. The results of the study showed that there were 4 types of bananas found in the Gorontalo Province which showed unique morphological variations, namely Kepok Bananas have the characteristic of quite large leaf morphology, Abati Bananas have a unique yellowish color, Green Butota Bananas have morphological characteristics with very thick fruit and Wild Bananas have characteristics with a large number of stones. Based on the results of the clustering, 2 main groups were formed which separated at a cosiness of 0.643 (64.3%) found between Abati Bananas, Green Butota, and Wild Bananas, while Kepok Bananas showed a very distant relationship with a cosiness value of 0.482 (48.2%). The results of this study have the potential to support the preservation of local varieties and support the sustainable genetics management through the identification of varieties that are tolerant to salinity and drought.

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