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Medicra (Journal of Medical Laboratory Science/Technology)
Published by Universitas Muhammadiyah Sidoarjo
ISSN : 25807730     EISSN : 25807730     DOI : https://doi.org/10.21070/medicra
Core Subject : Health,
Health Professions Public Health
Focus : to facilitate scholar, researchers, and lecturers for publishing the original articles of review articles. Scope : Medicra publishes research articles in the field of “medical laboratory (science/technology)” with the following scope: Clinic Chemical Hematology Microbiology Parasitology Immunology Food and beverage analysis Chemical Molecular Diagnostics Toxicology Cytology Histology Epidemiology Laboratory Management Laboratory Quality Control
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 8 Documents
 1 
Search results for , issue "Vol. 2 No. 1 (2019): July" : 8 Documents clear
Efektivitas Jeruk Lemon (Citrus limon Linn) terhadap Staphylococcus epidermidis: Effectiveness of Lemon (Citrus limon Linn) against Staphylococcus epidermidis Hartin, Ellyce; Rini, Chylen Setiyo
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 1 (2019): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i1.1617

Abstract

Acne or acne vulgaris is the one of the skin diseases that often appear in adolescents and adults. Acne is often associated with the condition of the body, either in stress condition because of sad or being happy. Acne can be caused by genetic, cosmetic, and bacterial factors. Bacteria that can cause swelling diseases is Staphylococcus epidermidis. For overcoming acne, people can use traditional medicine such as lemon juice (Citruslimon Linn). The main composition that exists in lemon juice (Citrus limon Linn) is citric acid that acts as an antimicrobial against bacteria present in acne. This study aims to determine the influence of lemon juice lime juice (Citrus limon Linn) against Staphylococcus epidermidis. This research is an experimental laboratory that use disc disk method or Kirby bauer method with concentration 0%, 12,5%, 25%, 50% and 100%. The result of the study shows that lemon lime juice (Citrus limon Linn) at 25% concentration already have a transparent zone around the paper disc about 12.1 mm and have low effectivity to secure Staphylococcus epidermidis. The high concentration of lemon juice will increase the inhibitory power produced by lemon juice (Citrus limon Linn).
Efektivitas Sabun Transparan Antibakteri Ekstrak Etanol Sawi Putih (Brassica Rapa Subspesies. Pekinensis) Dengan Kombinasi Coconut Oil dan Castor Oil: The Effectiveness of Antibacterial Transparent Soap Ethanol Extract of Chilli (Brassica Rapa Subspecies. Pekinensis) With a Combination of Coconut Oil and Castor Oil Khusniyah, Nurul; Rohmah, Jamilatur
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 1 (2019): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i1.1488

Abstract

This study aims to determine the best soap formula and to know the effect of addition of chicory extract (Brassica rapa subspecies, pekinensis) in transparent solid soap supply of antibacterial extract of chicory extract (Brassica rapa subspecies, pekinensis) to its effectiveness in Staphylococcus aureus. This research is experimental laboratory. Concentration of chicoryin soap are 0%, 25%, 50%, 75% and 100%. Anti-bacterial effectivenesstest was performed by disc method of transparent antibacterial soap of chicory extract of ethanol (Brassica rapa subspecies, pekinensis) and measured the clear zone. The analysis was performed by Two Way ANOVA test. The results of soap quality assurance standard fulfill quality standard specified by SNI in 1994 that water content test is found in formulation 6 (F6) with 75% concentration equal to 2.109%. The best free alkali test result were found in the formulation 5 (F5) with a 25% concentration of 0.041963, 50% by 0.041966 and 100% by 0.041963. The best free fatty acid test result were found in the formulation of 2 (F2) 50% concentration with free fatty acid level of 0.07852 %. For thebest pH test the conceration of 0%, 25% and 50% with pH yielded 9 and the best foam high yield test was found at 100% concentration. Result of Two Way ANOVA test with variation of extract concentration of chicory ethanol and combination of coconut oil and castor oil obtained p-value = 0.000 <0.05 which states there is real difference. The best test results are in F6 (30.6833 mm). Two Way ANOVA test results showed that transparentsolid soaps of chicory extracts had the best antibacterial effectiveness against Staphylococcus aureus with an inhibitory zone of 30.6833 mm at 100% concentration.
Pengaruh Filtrat Bawang Merah (Allium cepa) Terhadap Kadar Formalin pada Ikan Kembung (Rastrelliger sp): Effect of Shallot (Allium cepa) filtrate on Formalin levels in puffer fish (Rastrelliger sp) Anglania, Shintya Rara; Hanum, Galuh Ratmana
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 1 (2019): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i1.1654

Abstract

Fish contains of protein, vitamins, fats and minerals for the body health. Many manufacturers still use formaline for preservation in fish. Onion can reduce the formaline levels in fish with saponin. This study aims to analyze the efficacy of onion to decrease the formaline levels in mackerel fish. This research is experimental laboratory with several parameters, such as: formalin levels, water content, ash content and bacterial analysis. The samples were used 10%, 20%, 30%, 40% and 50% filtrate for 1, 3 and 5 days soaking. The result of the research showed 50% filtrate for 5 days can reduce formalin level is 0.10 mg/L.Water content with 10% filtrate for 1 day is the best result (12.15%), ash content with 10% filtrate with for 1 day is the best result (0.22%). The best results for bacterial analysis in 1 day with 0 colon. This study used Repeated Measure Anova statistical with variation of onion filtrates obtained p-value=0.000 (p<0.05). The results showed that onion filtrates potentially decreased formaline levels in mackerel fish.
Pengaruh Infusa Rimpang Temu Putih (Curcuma zedoaria (Berg.) Roscoe) Terhadap Jumlah Leukosit Dan Differential Counting (Diffcount) Pada Kesembuhan Luka Laparatomi Pasca Bedah: Effect of Infusion of Temu Putih Rhizome (Curcuma zedoaria (Berg.) Roscoe) on Leukocyte Count and Differential Counting (Diffcount) on Postoperative Laparotomy Wound Healing Wulandari, Ratna; Puspitasari, Puspitasari
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 1 (2019): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i1.1689

Abstract

Laparatomy is surgery performed to open the abdominal area which causes an increase in plasma adrenaline and inflammatory. Leukocytes will increase if the adrenal glands are stimulated as a physiological response such as stress and when active against microorganisms. Leukocytosis can trigger an increase in the number of neutrophil and lymphocyte cells in the blood circulation and cause an increase in leukocytes and diffcount. Curcuma zedoaria contain curcumin compounds as antioxidant effectiveness to prevent fisiologis stress, flavonoid and essential oils as an anti-inflammatory which can increase the total of leukocyte. The objective of this research is to determine the effect of several Curcuma zedoaria infusion on the total of leukocytes and diffcount on post operative laparatomy wound healing. A total of 24 Rattus norvegicus were used in this research. Group I (without infusion); group II (10% infusion); group III (15% infusion); group IV (20% infusion). Rattus norvegicus were sreened laparatomy and their blood was taken for leukocyte and differential counting. There is an effect of Curcuma zedoaria infusion on the number of leukocytes and diffcount. The best concentration 15% of leukocyte, and diffcount such as concentration 20% granulocyte, concentration 15% lymphocytes and concentration 10% monocyte in postoperative laparatomy wound healing.
Pengaruh Lama Penundaan Pemeriksaan Serum Terhadap Kadar Elektrolit Natrium dan Klorida: The Effect of Delayed Serum Examination on Sodium and Chloride Electrolyte Levels Azizah, Nurul; Aliviameita, Andika
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 1 (2019): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i1.2589

Abstract

Electrolytes play an important role in the human body that can affect metabolism. Sample handling more than two hours in examination electrolyte levels would lead to less accurate results. This study aims to determine the effect of long delays serum examination of the levels of electrolytes sodium and chloride for 0, 3, 5, and 7 hours. This research was conducted with an experimental method using 32 serum samples. Results of sodiumand chloride levels after an increase in deferred examination. The mean 0 hour sodium level is 142.55 mmol/L, 3-hour sodium level is 143.18 mmol/L, 5-hour sodium level is 143.91 mmol/L and 7-hour sodium level is 144.41 mmol/L. The mean 0 hour chloride level is 97.66 mmol/L, 3-hour chloride level is 99.96 mmol/L, 5- hour chloride level is 103.62 mmol/L and 7-hour chloride level is 107.55 mmol/L. This study reflected thatthere is no effect of delaying serum on sodium levels, and there is an effect of delaying serum on chloride levels.
Prevalensi Hepatitis B pada Komunitas Pria Homoseksual di Kota Bandung: Hepatitis B Prevalence in Homosexual Male Community in Bandung City Naully, Patricia Gita
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 1 (2019): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i1.2230

Abstract

Bandung is a city with the highest homosexual number in West Java. Homosexual is a term for someone having a sexual attraction to the same gender. The homosexual male community is more open to the public than the homosexual female. One of the health problems that is often suffered by the homosexual male community in various countries is Sexually Transmitted Infections (STIs) such as Hepatitis B. However, until now, the prevalence data of Hepatitis B in the community are very difficult to find in Indonesia. This study aimed to decide the prevalence of Hepatitis B in the homosexual male community in Bandung. The sample used in this study was 40 people. All samples were homosexual males fulfilling the inclusion criteria. The presence of Hepatitis B virus Surface Antigen (HBsAg) was detected by sandwich qualitative Enzyme-Linked Immunosorbent Assay (ELISA) method. Laboratory test results showed that 13 homosexual males were infected by the Hepatitis B virus. The majority of the sufferers have had risky sexual relations, such as changing partners, not using a condom, doing anal and oral sex. In addition, based on the interview results, it was known that all infected people have not received the hepatitis B vaccination when they are adult. Moreover, based on this study, it can be concluded that the prevalence of Hepatitis B in the homosexual male community in Bandung was 32.5%.
Penentuan Konsentrasi Ammonium dalam Air Sungai Pelayaran Ngelom: Determination of Ammonium Concentration in Ngelom . Shipping River Water Ngibad, Khoirul
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 1 (2019): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i1.2071

Abstract

Ammonium (NH4+) is a form of ammonia (NH) compound in thewater at low pH. The ammonium can cause poisoning in aquatic life. The purpose of this study was to determine the ammonium concentration in the Ngelom river water sample that flows in the Ngelom village, Taman district, Sidoarjo Regency. The measurement of ammonium concentration in river water samples was carried out using the UV-Vis spectrophotometrymethod with Nessler reagent based on the Nessler reagent reaction (K2HgI4) with ammonium in a base solution which would form a colloidal yellow brown dispersion which absorbance measured at 410 nm. Ammonium absorbance is proportional to the ammonium concentration in the sample. The sampling technique in this study used purposive sampling. Samples were taken at 10 trial points, every 5 meters to the right and left from the center point (Ngelom's River bridge to Maarif Hasyim Latif University). Ammonium concentration was determined in the linear concentration range of 1 – 5 mg/L with a correlation coefficiency value (r) of 0.9615 with limit of detection and limit of quantitation of 0.105 mg/L and 0.352 mg/L, respectively. The results of testing the river water samples showed that the ammonium concentration in 10 samples of river water ranged from 1.61 – 14.68 mg/L. All of the sample was not eligible requirements of the Republic of Indonesia PP No. 82 of 2001 about Water Quality Management and Water Pollution Control.
Ekstrak Daun Keji Beling (Strobilanthes crispus L.) Uuntuk Penurunan Kadar Kolesterol Pada Tikus Putih Jantan (Ratus norvegicus) Galur Wistar: Keji Beling (Strobilanthes crispus L.) Leaf Extract for Reducing Cholesterol Levels in Male White Rats (Ratus norvegicus) Wistar strain Rachman, Fadila Abd; Ardiansyah, Syahrul
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 1 (2019): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i1.1648

Abstract

Indonesian citizens have a various lifestyle impacts on the increasing of blood-cholesterol level. One of the potential herbal medicine is keji beling leaves (Strobilanthes crispus L.) because of these leaves contains flavonoid and sterol. This study aims to analyze the efficacy of Strobilanthes crispus L. leaves extract to decrease the blood-cholesterol level. This research is experimental laboratory with pretest – post test only control group design. The total of 24 Wistar rats were divided into six groups, as follow: negative control, positive control, treated with 25%, 50%, 75% and 100% Strobilanthes crispus L. extract. The therapy was administrated for 14 days. Cholesterol test using POCT method. The data were analyzed using Repeated Measured Anova. The results showed that Strobilanthes crispus L. leaves extracts potentially decreased on blood-cholesterol level in Wistar rats after 14 days of treatment. This study reflected that the Strobilanthes crispus L. leaves extract can be used as the alternative therapy on the blood-cholesterol level. The greatest decrease in P4 (100% concentration).

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