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INDONESIA
HAYATI Journal of Biosciences
Published by Institut Pertanian Bogor
ISSN : 19783019     EISSN : 20864094     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
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Articles 10 Documents
 1 
Search results for , issue "Vol. 27 No. 3 (2020): July 2020" : 10 Documents clear
Ecological Diversity of Microbial Consortium Feces of Beef Cattle and Lignite Coal Gina Chynthia Kamarudin Puteri; Roni Ridwan; Ellin Harlia
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.180

Abstract

Increasing energy demand is not alongside the availability of limited fossil fuels. Alternative and renewable energy sources are not only an option to overcome energy problems but also essential to minimize global warming. Another critical and promising renewable energy source is biomass-derived from livestock feces. Beef cattle feces contain a microorganism consortium that can be used as a starter with coal media to form biogas. Indonesia recently developed coal waste processing into renewable energy, such as biogas. This study aimed to overview the ecological diversity of microbial consortium of beef cattle feces, lignite coal waste, and a combination of livestock and lignite coal waste under mesophilic conditions. This research is an explorative method, the data obtained were analyzed descriptively. The process of formation was carried out anaerobically on a bottle containing the rumen fluid medium. The fermentation process lasted 42 days at 39℃ of temperature. After that, the sample was electrophoresis, followed by next-generation sequencing (NGS) method. NGS data is processed with the MG-Rast website. This study demonstrates the ecological diversity of microbial consortium of beef cattle, lignite coal waste, and a combined consortium. The results showed ecological diversity in the form of taxonomy dominated by bacteria, eukaryotes, and archaea.
Apoptotic Potential of Secretome from Interleukin-Induced Natural Killer Cells toward Breast Cancer Cell Line by Transwell Assay Wahyu Widowati; Diana Krisanti Jasaputra; Teresa Liliana Wargasetia; The Fransiska Eltania; Alya Mardhotillah Azizah; Mawar Subangkit; I Nyoman Ehrich Lister; Chrismis Novalinda Ginting; Ermi Girsang; Ahmad Faried
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.186

Abstract

Breast cancer (BC) is the number one cause of deaths from cancer in women. Metastasis in BC is caused by immunosurveillance deficiency, including impairment of Natural Killer (NK) cell maturation, low NK activity, and decreasing cytotoxicity. This study was performed to improve activating receptors and cytotoxicity of NK cells using interleukin 15 (IL15) against BC cells. Human recombinant IL15 was used to induce NK cells. To evaluate the potential of IL15 in inducing NK cells, we measured the activating and inhibiting receptors (NKG2D, NKG2A), apoptotic potency of NK cells on BC cells (MCF7) using transwell assay. The IL15 inducer on the NK cell were measured NKG2D, NKG2A gene expression with quantitative polymerase chain reaction (qPCR), (GzmB) secretion using ELISA, apoptotic gene expression of MCF7 using qPCR. IL15 increased NKG2D expression 4.01-9.13%, but IL15 could not affect toward NKG2A expression on NK cells. IL15-activated NK cells, inhibited BC cells proliferation, induced apoptotic BC cells 25.89-32.19%, induced apoptotic genes of BC cells bax, p53. IL15 increase NK activating receptor (NKG2D), inhibit BC cells proliferation, induce apoptotic percentage and induce apoptotic gene expression.
Impact of Methanotrophic and N2O-reducing Bacterial Inoculation on CH4 and N2O Emissions, Paddy Growth and Bacterial Community Structure in Paddy Field Yuli Siti Fatma; Iman Rusmana; Aris Tri Wahyudi; Hamim Hamim
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.197

Abstract

Paddy field is one of the anthropogenic sources that produce greenhouse gases emission. This study aimed to investigate the impact of methanotrophic and N2O-reducing bacterial inoculation on CH4 and N2O emissions, paddy growth and bacterial community structure in paddy field. Two treatments of 100% synthetic fertilizer (250 kg urea/ha) without biofertilizer and 50% synthetic fertilizer (125 kg urea/ha) with biofertilizer consisted of methanotrophic and N2O-reducing bacteria were applied in the paddy field. Inoculation of methanotrophic and N2O-reducing bacteria was able to reduce CH4 and N2O emission up to 4.19 mg CH4/m2/day and 351.29 µg N2O/m2/day, respectively. Those bacterial applications were also able to increase paddy growth and yield productivity. According to DGGE profile, inoculation of the biofertilizer seemed to have a transient impact on bacterial communities in paddy soil at 36 days after transplanting (DAT) which showed the lowest similarity with all samples (a similarity index of 0.68). DGGE bands successfully excised have closest relative to uncultured bacteria which comprised 5 phyla, i.e. Proteobacteria (Alphaproteobacteria and Deltaproteobacteria), Nitrospirae, Actinobacteria, Firmicutes, and Acidobacteria. In this study, Alphaproteobacteria was the most dominant phylum. We provide basic information for developing the biofertilizer which supports sustainable agriculture.
Antagonistic Activity of Fungal Endophytes Isolated from Garcinia atroviridis against Colletotrichum gloeosporioides Nur Afeeqah Binti Mohamed Zanudin; Nor'Aishah Hasan; Patahayah Binti Mansor
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.209

Abstract

The extensive use of synthetic fungicides in controlling plant disease generates detrimental impacts on the environment and human health. In response to this problem, an alternative method was developed, known as biological control using antagonistic microorganisms. Since investigation on fungal endophytes of Garcinia atroviridis is still unclear, it was chosen for the study. The aim of the present work was to evaluate biocontrol potential of endophytic fungi against Colletotrichum gloeosporiodes, a phytopathogen that caused anthracnose disease. A total of 92 endophytic fungi were isolated from different tissue parts of Garcinia atroviridis including leaves, petioles, branches, and fruits. Results demonstrated that, most of endophytic fungal isolates showed some inhibitory action over the growth of C. gloeosporiodes during dual culture growth. Endophyte isolate F14 showed the highest antagonistic activity against Colletotrichum gloeosporiodes with 67.38% percentage inhibition radial growth (PIRG). However, 7 out of 92 isolates showed no inhibitory effect against Colletotrichum gloeosporiodes. In conclusion, endophytic fungi isolated from G. atroviridis indicate the potential as biocontrol agents. It is hoped that the finding of isolated endophytic fungi in this study with antagonistic activity against anthracnose pathogen may be used in biocontrol programmes of plant disease in the region.
Screening of Endophytic Bacteria as Biocontrol Agents Against Bacteria Leaf Blight (Xanthomonas oryzae) Rashidah Abd Halim; Nor'Aishah Hasan; Kogeethavani Ramachandran
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.215

Abstract

Bacterial leaf blight (BLB) is one of major threats in rice production as it can cause 100% yield loss. Concern on the environment and human health has led an attempt to replace existing methods of chemical control and avoid extensive use of bactericides by using endophytic bacteria. The present study was conducted to screen and characterize bacteria isolated from different sources that has potential as antagonistic bacteria against Xanthomonas oryzaepv. oryzae (Xoo), the causal agent of bacterial leaf blight of paddy. Two hundred and thirty-three endophytic bacteria were successfully isolated from roots and leaves from paddy field. Only 17 endophytic bacterial isolates showed positive antagonistic activity indicated by inhibition zone around bacterial colony against Xoo on nutrient agar plate with 2 endophytic isolates (BCA 3 and BCA 12) showed highest inhibitory effect with 35±0.00 mm in diameter. Molecular identification by 16S rRNA amplification successfully identified the antagonistic endophytic bacteria as Pseudomonas fluorescensand Geobacillus thermoparaffinivorans. Findings in this study revealed the biocontrol abilities of isolated endophytes as an excellent option to be used by agriculture sectors to have sustainable environment.
Mammalian Diversity of Gunung Ledang, Johor, Peninsular Malaysia Faiznur Ain Ahmad Bakri; Masatoshi Yasuda; Maryati Mohamed; Adlil Ikram Sharuddin; Muhazam Syah Hambar
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.221

Abstract

Mammals play a crucial role for the biotic processes of the rainforest ecosystem. Studying their behaviour and biology are vital to fully understand the jigsaw of rainforest dynamics. This study was made to update the diversity checklist of mammals in Gunung Ledang. This study was done in Taman Negara Johor; Gunung Ledang (2.37°N, 102.60°E) where it is known to be the highest mountain (1,276 m asl.) in the southern Malay Peninsula. This study was conducted from July 2018 to April 2019. In total, 22 cameras were installed along three nature trails which are Sagill trail, Telekom Trail, and Resort Trail. Overall, a total of 31 species of terrestrial mammals from small- to large-sized were recorded in our camera trapping study of 3434 camera-days.Mammals such as the Leopard (Panthera pardus), Sumatran Serow (Capricornis sumatraensis), and Malayan Tapir (Tapirus indicus) were caught in the videos of camera trap. This showed that the diversity of mammals in Gunung Ledang is quite high. Tthis paper already gave valuable insights in a rainforest guild that is highly diverse and of outstanding value to the forest ecosystem. Management plans and strategy to conserve the mammals in Gunung Ledang can be done as accordingly afterwards.
In silico Vaccine Design against Dengue Virus Type 2 Envelope Glycoprotein Muhammad Adnan; Matin Nuhamunada; Lisna Hidayati; Nastiti Wijayanti
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.228

Abstract

Dengue fever is caused by the mosquito-borne virus termed (DENV). However, DENV-2 has been identified as the most prevalent amongst the Indonesian pediatric urban population, in contrast with the other four serotypes. Therefore, it is important to reduce severe infection risk by adopting preventive measures, including through vaccine development. The aim of this study, therefore is to use various in silico tools in the design of epitope-based peptide vaccines (T-cell and B-cell types), based on the DENV-2 envelope glycoprotein sequences available. Therefore, in silico methods were adopted in the analysis of the retrieved protein sequences. This technique was required to determine the most immunogenic protein, and is achieved through conservancy analysis, epitope identification, molecular simulation, and allergenicity assessment. Furthermore, B4XPM1, and KAWLVHRQW were identified from positions 204-212, while the 77 to 85 peptide region was considered the most potent T-cell and B-cell epitopes. The interaction between KAWLVHRQW and HLA-C*12:03 occurs with maximum population coverage, alongside high conservancy (96.98%) and binding affinity. These results indicated a potential for the designed epitopes to demonstrate high immunity against DENV-2.
Identification of Follower Status Based on Male Proximity Score in Crested Macaque Andre Pasetha; Lisa Michelle Danish; Dyah Perwitasari-Farajallah; Muhammad Agil; Antje Engelhardt
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.241

Abstract

Crested macaque live in multimale-multifemale social groups where temporary association (consortship) typically occurs. Current theory and these limited qualitative observations suggest the hypothesis that behavior functions as a means for males to gain access to fertile females. The aim of this study was to investigate follower status based on quantitative method. Males were classified as either “consort males,” “followers,” and “non-followers” based on proximity maintanance every 15 minute uses scan sampling. Tactics used by followers were classified into 1) individual challenge, 2) coalitionary challenge, 3) abandoned takeover, and 4) opportunistic takeover. The proportion of successful takeovers by followers was calculated by dividing the number of takeovers by followers by the total number of observed takeovers. The proportion of followers is higher than average on D-5 and earlier, D-4, and D-3. Only two of the four consort takeover tactics were used by followers. For abandoned which made up 40% and for individual tactic was made up to 11.5% of consort takeovers tactic used. This study contribute to our understanding of alternative mating strategy in primate and provide the first quantitative data demonstrating that following is an alternative mating strategy in crested macaque (Macaca nigra).
Anatomical Properties and Near Infrared Spectra Characteristics of Four Shorea Species from Indonesia Danang Sudarwoko Adi; Sung-Wook Hwang; Dwi Ajias Pramasari; Yusup Amin; Hairi Cipta; Ratih Damayanti; Wahyu Dwianto; Junji Sugiyama
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.247

Abstract

This study investigated the anatomical properties and absorbance characteristics of NIR spectra of four Shorea species from Indonesia. Macroscopic section revealed that Balau has similarity with Heavy Red Meranti, whereas White Meranti was almost identical with Light Red Meranti. All of the woods have diffuse porous and axial resin canals in tangential lines at the microscopic level. Original NIR spectra of Shorea species showed different absorbance characteristic. Wood density was assumed to be one of the factors that affected to the absorbances. Principal component analysis (PCA) of second derivative NIR spectra at the wavenumber 8,000-4,000 cm-1 (full) and 6,200-5,600 cm-1 (specific) showed different orientation among the Principal Component (PC) number. PC1, which contained highest spectral variation, had two closed clusters (1) Balau and Heavy Red Meranti and (2) White and Light Red Meranti at full spectral range. In contrast, the results at specific range were (1) Balau and White Meranti and (2) Heavy and Light Red Meranti. Hierarchical clustering dendrogram using PCA data from two spectral regions resulted in two types of clustering, the 8,000-4,000 cm-1 was somehow related to ‘density’, while the 6,200-5,600 cm-1 was grouped in ‘color’ information from visual inspection. From both spectral regions, k-nearest neighbour (k-NN) classification models revealed 100% accuracy in identification four Shorea species using NIR spectra.
Genetic Relationship between Tongka Langit Bananas (Musa troglodytarum L.) from Galunggung and Maluku, Indonesia, Based on ITS2 Fenny Martha Dwivany; Giasintha Stefani; Agus Sutanto; Husna Nugrahapraja; Ketut Wikantika; Adriana Hiariej; Topik Hidayat; I Nyoman Rai; Nisrina Sukriandi
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.258

Abstract

Tongka Langit or Fe’i banana (Musa troglodytarum L.) has the T genome and a very high content of beta-carotene. It only grew and spread around the regions of Maluku islands and Papua. However, recently our team found this banana on the foot of mount Galunggung, West Java, so this raised the question about its origin. The objective of this study was to understand the genetic relationship between Tongka Langit from Galunggung and Maluku islands and compared it with other bananas with different genomes. Genetic diversity analysis was done using ITS2 DNA marker and dendrogram analysis showed three groups. From the comparison of the ITS2 sequences, there were no difference (100% identity) between the ITS2 sequence of Tongka Langit originating from Galunggung and Maluku. In conclusion, based on the ITS2 marker, the Tongka Langit were more distantly related to cultivars with A and B genomes, and there was no difference in the ITS2 sequence of Tongka Langit originating from Galunggung and Maluku. To the best of our knowledge, there is no previous report of genetic relationship between Tongka Langit from Galunggung and other regions.

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