Garuda - Garba Rujukan Digital

Zingiber officinale, Piper retrofractum and Combination Induced Apoptosis and p53 Expression in Myeloma and WiDr Cell Lines HENY EKOWATI; ANISYAH ACHMAD; EKA PRASASTI; HENDRI WASITO; KADEK SRI; ZULIA HIDAYATI; TUTI EKASARI
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

In previous studies, Zingiber officinale, Piper retrofractum, and the combination showed cytotoxic activity, induced apoptosis, and p53 expression of HeLa, T47D, and MCF-7 cell lines. This study was conducted to investigate the cytotoxic and apoptotic activity of Zingiber officinale (ZO), Piper retrofractum (PR), and the combination as well as their effect to p53 expression on Myeloma and WiDr cells. The powder of ZO, PR, and ZO + PR combination (1:1) were macerated with 96% ethanol for 3 x 24 hours. MTT cytotoxic assay was performed on Myeloma and WiDr cell lines. Apoptotic cells were stained with ethidium bromide and acridine orange. Imunohistochemical expression of p53 was examined on Myeloma and WiDr cell lines. Doxorubicin was used as positive control in all assays. Results showed that ZO, PR, and ZO + PR combination had cytotoxic activity on Myeloma cells with IC50 of 28, 36, and 55 mg/ml respectively and WiDr cell lines with IC50 of 74, 158, and 64 mg/ml respectively, induced apoptotic activity, and increased p53 expression on Myeloma and WiDr cells. These results suggest that ZO, PR, and their combination induced Myeloma and WiDr cells in apoptosis through p53 expression.

Genetic Diversity of Acacia mangium Seed Orchard in Wonogiri Indonesia Using Microsatellite Markers VIVI YUSKIANTI; KEIYA ISODA
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Genetic diversity is important in tree improvement programs. To evaluate levels of genetic diversity of first generation Acacia mangium seedling seed orchard in Wonogiri, Central Java, Indonesia, three populations from each region of Papua New Guinea (PNG) and Queensland, Australia (QLD) were selected and analyzed using 25 microsatellite markers. Statistical analysis showed that PNG populations have higher number of detected alleles and level of genetic diversity than QLD populations. This study provides a basic information about the genetic background of the populations used in the development of an A. mangium seed orchard in Indonesia.

Yeast Isolation for Bioethanol Production EKA RURIANI; TITI CANDRA SUNARTI; ANJA MERYANDINI
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

We have isolated 12 yeast isolates from five different rotten fruits by using a yeast glucose chloramphenicol agar (YGCA) medium supplemented with tetracycline. From pre-screening assay, four isolates exhibited higher substrate (glucose-xylose) consumption efficiency in the reaction tube fermentation compared to Saccharomyces cerevisiae dan Saccharomyces ellipsoids as the reference strains. Based on the fermentation process in gooseneck flasks, we observed that two isolates (K and SB) showed high fermentation efficiency both in sole glucose and mixed glucose-xylose substrate. Moreover, isolates K and SB produced relatively identical level of ethanol concentration compared to the reference strains. Isolates H and MP could only produce high levels of ethanol in glucose fermentation, while only half of that amount of ethanol was detected in glucose-xylose fermentation. Isolate K and SB were identified as Pichia kudriavzeevii (100%) based on large sub unit (LSU) ribosomal DNA D1/D2 region.

Archaeal Life on Tangkuban Perahu- Sampling and Culture Growth in Indonesian Laboratories SRI HANDAYANI; IMAN SANTOSO; HANS-JOACHIM FREISLEBEN; HARALD HUBER; . ANDI; FERY ARDIANSYAH; CENMI MULYANTO; ZESSINDA LUTHFA; ROSARI SALEH; SERUNI KUSUMA UDYANINGSIH FREISLEBEN; SEPTELIA INAWATI WANANDI; MICHAEL THOMM
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

The aim of the expedition to Tangkuban Perahu, West Java was to obtain archaeal samples from the solfatara fields located in Domas crater. This was one of the places, where scientists from the University of Regensburg Germany had formerly isolated Indonesian archaea, especially Thermoplasma and Sulfolobus species but not fully characterized. We collected five samples from mud holes with temperatures from 57 to 88 oC and pH of 1.5-2. A portion of each sample was grown at the University of Regensburg in modified Allen’s medium at 80 oC. From four out of five samples enrichment cultures were obtained, autotrophically on elemental sulphur and heterotrophically on sulfur and yeast extract; electron micrographs are presented. In the laboratories of Universitas Indonesia the isolates were cultured at 55-60 oC in order to grow tetraetherlipid synthesizing archaea, both Thermoplasmatales and Sulfolobales. Here, we succeeded to culture the same type of archaeal cells, which had been cultured in Regensburg, probably a Sulfolobus species and in Freundt’s medium, Thermoplasma species. The harvested cells are documented by phase contrast microscope equipped with a digital camera. Our next steps will be to further characterize genetically the cultured cells from Tangkuban Perahu isolates.

Xanthomonas oryzae pv. oryzae (Xoo), a causal agent of bacterial leaf blight (BLB), is one of the most important pathogens of rice. The effectiveness of ten Streptomyces spp. isolates in suppressing Xoo disease was assessed in planta and in vitro. In planta experiments were carried out in a greenhouse and arranged in a randomized completely block design (RCBD) with three replications. Twenty treatments were tested which included plants inoculated with both Streptomyces spp. and Xoo, and plants i RATIH DEWI HASTUTI; YULIN LESTARI; ANTONIUS SUWANTO; RASTI SARASWATI
HAYATI Journal of Biosciences Vol. 19 No. 4 (2012): December 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.19.4.155

Xanthomonas oryzae pv. oryzae (Xoo), a causal agent of bacterial leaf blight (BLB), is one of the most important pathogens of rice. The effectiveness of ten Streptomyces spp. isolates in suppressing Xoo disease was assessed in planta and in vitro. In planta experiments were carried out in a greenhouse and arranged in a randomized completely block design (RCBD) with three replications. Twenty treatments were tested which included plants inoculated with both Streptomyces spp. and Xoo, and plants inoculated with only Streptomyces spp. Plants inoculated with Xoo and sprayed with a chemical bactericide, and plants inoculated with only Xoo served as positive controls, whereas plants not inoculated with either Streptomyces spp. or Xoo were used as negative controls. The results showed that the effect of endophytic Streptomyces spp. on BLB disease expressed as area under disease progress curve (AUDPC) was not significantly different to that on control plants (P > 0.05). However, plants inoculated with endophytic Streptomyces spp. were significantly taller and produced higher tiller number than control plants (P < 0.05). Streptomyces spp. isolate AB131-1 gave the highest plant height. In vitro studies on biocontrol mechanisms of selected Streptomyces spp. isolates showed that isolate LBR02 gave the highest inhibition activity on Xoo growth, followed by AB131-1 and AB131-2. Two isolates (AB131-1 and LBR02) were able to produce chitinase, phosphatase, and siderophore which included biocontrol characteristics. Morphological and colonization studies under SEM and light microscopy confirmed that the three isolates were endophytic Streptomyces spp. from different species. These studies found that the paddy plant which was inoculated with endophytic Streptomyces spp. AB131-1 and infected by Xoo could increase the height of plant and number of tillers.

Effect of phospholipids on survival and molting synchronicity of mud crab larvae Scylla serrata were examined using Artemia enriched with five treatments of emulsion oil i.e. treatment with different level of soybean lecithin (SL) together with a level of DHA70G (referred to as DHA-SL0, 20 and 40) and treatment with SL and cuttle fish phospholipids (CPL) at 40 uL/L without DHA70G (referred to as WDHA-SL and WDHA-CPL). Survival rate, intermolt period, carapace width, and molting synchronicity wer MUHAMMAD AGUS SUPRAYUDI; TOSHIO TAKEUCHI; KATSUYUKI HAMASAKI
HAYATI Journal of Biosciences Vol. 19 No. 4 (2012): December 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.19.4.163

Effect of phospholipids on survival and molting synchronicity of mud crab larvae Scylla serrata were examined using Artemia enriched with five treatments of emulsion oil i.e. treatment with different level of soybean lecithin (SL) together with a level of DHA70G (referred to as DHA-SL0, 20 and 40) and treatment with SL and cuttle fish phospholipids (CPL) at 40 uL/L without DHA70G (referred to as WDHA-SL and WDHA-CPL). Survival rate, intermolt period, carapace width, and molting synchronicity were evaluated. Additionally, lipid classes and fatty acid composition of enriched Artemia were analyzed. Survival rate, intermolt period, and carapace width at the first crab (FC) stage of mud crab larvae fed DHA-SL0 to 40 were similar to that of WDHA-CPL but higher than that of WDHA-SL (P < 0.05). Moreover, mud crab larvae fed DHA-SL20, DHA-SL40, and WDHA-CPL had a significantly higher molting synchronicity index compared to that of larvae fed WDHA-SL and DHA-SL0. It can be concluded that combination of phospholipids and essential fatty acids exhibited an additive effect in improving molting synchronicity of mud crab larvae.

Two clones of N1 gene derived from isolate A/Dk/Tangerang/Bbalitvet-ACIAR-TE11/2007 (H5N1) exhibit single mismatch of amino acid sequence at position 242 that is threonine and methionine for the clone #3 and #5, respectively. In order to evaluate the effect of the amino acid substitution, these clones were inserted into two different expression vectors that are pEGFP-C1 and pcDNA-3.3 TOPO® TA cloning. Subsequently, the respective recombinant clones were transfected into eukaryotic cells, includi RISZA HARTAWAN; NI LUH PUTU INDI DHARMAYANTI; KARL ROBINSON; TIMOTHY MAHONY; JOANNE MEERS
HAYATI Journal of Biosciences Vol. 19 No. 4 (2012): December 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.19.4.169

Two clones of N1 gene derived from isolate A/Dk/Tangerang/Bbalitvet-ACIAR-TE11/2007 (H5N1) exhibit single mismatch of amino acid sequence at position 242 that is threonine and methionine for the clone #3 and #5, respectively. In order to evaluate the effect of the amino acid substitution, these clones were inserted into two different expression vectors that are pEGFP-C1 and pcDNA-3.3 TOPO® TA cloning. Subsequently, the respective recombinant clones were transfected into eukaryotic cells, including CEF, RK13 and VERO using Lipofectamine ‘plus’ reagent. As a result, the clone #3 retaining atypical sequence showed lower expression level rather than the clone #15 in both vectors and all type of cells. The 3D conformational modelling revealed that the mutation occurs in the inner part of glycoprotein embedded within envelope or matrix. Therefore, the missense mutation seems has no effect on the antigenic properties of neuraminidase but this substitution by any means causes lethal mutagenesis in the individual gene expression by reducing level of protein transcript.

The prevalence of V.ovalifolium in Pulau Dua Nature Reserve was surveyed. This parasite was observed parasitizing on only two host species, Excoecaria agallocha and Thespesia populnea, among 11 potential host tree species in the study area. This phenomenon, contemporarily coined as local host specificity, is complementary to a small endnote on Backer & Bakhuizen van den Brink’s 1965 Flora of Java vol. II. V.ovalifolium’s prevalence is higher as the increase on host tree DBH, as the decrease of i AGUNG SEDAYU; ALEX SUMADIJAYA
HAYATI Journal of Biosciences Vol. 19 No. 4 (2012): December 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.19.4.177

The prevalence of V.ovalifolium in Pulau Dua Nature Reserve was surveyed. This parasite was observed parasitizing on only two host species, Excoecaria agallocha and Thespesia populnea, among 11 potential host tree species in the study area. This phenomenon, contemporarily coined as local host specificity, is complementary to a small endnote on Backer & Bakhuizen van den Brink’s 1965 Flora of Java vol. II. V.ovalifolium’s prevalence is higher as the increase on host tree DBH, as the decrease of infested host branch diameter and as the increase of host tree branching order. Two later findings point at Dicaeum trochileum as V.ovalifolium seed disperser among 90 bird species living on the nature reserve.

Visually identifying the sex of a bird can be difficult. It cannot be done in half the world’s species when they are adults, and virtually none can be sexed as chicks. Despite this, the sex of a bird is vital for captive breeding. An increased number of birds are being sexed using DNA amplification techniques. In this approach, the CHD-W and CHD-Z are distinguished by the amplification of an intron present in both genes. PCR products on the gel electrophoresis vary in size revealing one band in SRI SULANDARI; MOCH SYAMSUL ARIFIN ZEIN
HAYATI Journal of Biosciences Vol. 19 No. 4 (2012): December 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.19.4.183

Visually identifying the sex of a bird can be difficult. It cannot be done in half the world’s species when they are adults, and virtually none can be sexed as chicks. Despite this, the sex of a bird is vital for captive breeding. An increased number of birds are being sexed using DNA amplification techniques. In this approach, the CHD-W and CHD-Z are distinguished by the amplification of an intron present in both genes. PCR products on the gel electrophoresis vary in size revealing one band in males at the CHD-Z, and two bands in females corresponding to both the CHD-W and CHD-Z. Two independent sets of primer (P8/P2 and 2550F/2718R) were used to amplify the CHD gene region from both the Z and W chromosome. One hundred and ten (110) birds were sexed using first pair of primers: (P8/P2). Sexing results indicated that 81.8% were successfully determined, 12.7% failed to be amplified and 5.5% were not perfectly determined because the PCR products showed thick band. The thick band caused misidentified female to male birds. An alternative primer (2550F/2718R) was applied to solve the problem. Two hundreds and twenty-nine birds were sexed and the results showed 100% successfully determined. From this study, it is suggested to use a pair of 2550F and 2718R primers for distinguishing a male from a female bird.

Distribution of fish in river is controlled by physico-chemical properties of the water which is affected by land-use complexity and intensity of human intervention. A study on fish distribution was carried out in the upper Citarum River to map the effects of physio-chemical properties on habitat use. A survey was conducted to collect fish and to measure the water quality both on dry and rainy season. The result showed that distribution of the fish, in general, represented their adaptive respons . SUNARDI; KEUKEU KANIAWATI; TEGUH HUSODO; DESAK MADE MALINI; ANNISA JOVIANI ASTARI
HAYATI Journal of Biosciences Vol. 19 No. 4 (2012): December 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.19.4.191

Distribution of fish in river is controlled by physico-chemical properties of the water which is affected by land-use complexity and intensity of human intervention. A study on fish distribution was carried out in the upper Citarum River to map the effects of physio-chemical properties on habitat use. A survey was conducted to collect fish and to measure the water quality both on dry and rainy season. The result showed that distribution of the fish, in general, represented their adaptive response to physico-chemical properties. The river environment could be grouped into two categories: (i) clean and relatively unpolluted sites, which associated with high DO and water current, and (ii) polluted sites characterized by low DO, high COD, BOD, water temperature, NO3, PO4, H2S, NH3, and surfactant. Fish inhabiting the first sites were Xiphophorus helleri, Punctius binotatus, Xiphophorus maculatus, and Oreochromis mossambicus. Meanwhile, the latter sites were inhabited by Liposarcus pardalis, Trichogaster trichopterus, and Poecilia reticulata. Knowledge about fish distribution in association with the pysico-chemical properties of water is crucial especially for the river management.

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
hayati_j_biosci@cbn.net.id

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official hayati_j_biosci@cbn.net.id

Editorial Address -

Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
hayati_j_biosci@cbn.net.id

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA