Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

HAYATI Journal of Biosciences

hayati
Website

Published by Institut Pertanian Bogor

ISSN : 19783019 EISSN : 20864094 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.

Arjuna Subject : -

Articles 1,077 Documents

23 24 25 26 27

Evaluation of Biofloc Technology Application on Water Quality and Production Performance of Red Tilapia Oreochromis sp. Cultured at Different Stocking Densities . WIDANARNI; JULIE EKASARI; SITI MARYAM
HAYATI Journal of Biosciences Vol. 19 No. 2 (2012): June 2012
Publisher : Bogor Agricultural University, Indonesia

This study evaluated the effect of biofloc technology (BFT) application on water quality and production performance of red tilapia Oreochromis sp. at different stocking densities. Three different fish densities were applied, i.e. 25, 50, and 100 fish/m3, and for each density there were Control (without external carbon input) and BFT treatments. Mixed sex red tilapia with an initial average body weight 77.89 + 3.71 g was cultured in 3 m3 concrete tanks for 14 weeks. Molasses was added on BFT treatments as the organic carbon source at a C/N ratio of 15. Control treatments of each density tested showed more fluctuated water quality parameters throughout the experimental period. The highest TAN and nitrite-nitrogen were observed in control treatment at a stocking density of 100 fish/m3 (3.97 mg TAN/L and 9.29 mg NO2-N/L, respectively). The highest total yield was observed in control treatment at the highest density treatment (43.50 kg), whereas the highest survival was obtained by BFT treatment at a density of 25 fish/m3 (97.78 + 0.77%). Total feed used in BFT was lower than that of control treatments in particular at 50 fish/m3 density (P < 0.05) suggesting that biofloc could be continuously harvested by the fish as other source of food.

Molecular Regulation of Noradrenaline in Bovine Corpus Luteum ANOM BOWOLAKSONO
HAYATI Journal of Biosciences Vol. 19 No. 2 (2012): June 2012
Publisher : Bogor Agricultural University, Indonesia

Noradrenergic stimulation increases progesterone, oxytocin and prostaglandins in the bovine luteal tissue. Better understanding of noradrenaline (NA) role in bovine the corpus luteum (CL) can advance our current knowledge on the regulatory mechanism of CL function. The present study was conducted to explore the source of noradrenaline and further to investigate whether nerve growth factor (NGF), insulin like growth factor 1 (IGF1) and transforming growth factor b1 (TGFb1) influence the expression of dopamine-b-hydroxylase (DBH), biosynthetic enzyme of NA in cultured bovine luteal cells. Corpora lutea were collected and classified into stages of early, developing, mid, late, and regressed. Messenger RNA (mRNA) and protein expression of DBH were studied throughout the estrous cycle. Additionally, DBH protein expression was examined in cultured mid luteal cells after tumour necrosis factor alpha/interferon gamma (TNFa/IFNg)-induced apoptosis or after treatment with NGF, IGF1, and TGFb1. DBH mRNA and protein expressions were detected throughout the cycle without significant changes in the protein level while mRNA showed a decrease at the developing stage (P < 0.05). Interestingly, NGF, IGF1, and TGFb1 increased DBH expression in cultured luteal cells (P < 0.05). The overall findings suggest non-neural source of noradrenaline in the bovine CL which appears to be regulated by NGF, IGF1, and TGFb1 indicating intraluteal molecules play an important and unrecognized role in the CL function.

Effect of Carbohydrate Source on Growth and Performance of In Vitro Sago Palm (Metroxylon sagu Rottb.) Plantlets . SUMARYONO; WIRDHATUL MUSLIHATIN; DIAH RATNADEWI
HAYATI Journal of Biosciences Vol. 19 No. 2 (2012): June 2012
Publisher : Bogor Agricultural University, Indonesia

Sago palm (Metroxylon sagu Rottb.), grown mostly in the tropics, is one of the most productive carbohydrate-producing crops. However, it is still underutilized. Tissue culture of sago through somatic embryogenesis has been developed. The plantlets derived from somatic embryos, however, are usually weak with few leaves and roots and have low survival rates during acclimatization. Carbohydrate is commonly added into culture medium as an energy source and an osmotic agent. Research was conducted to determine a suitable carbohydrate for plantlets growth in order to produce vigorous plantlets of sago. The basal medium used was a modified MS medium with a half-strength of salts. Different types of carbohydrate (sucrose, maltose, glucose, and fructose) at various concentrations (30, 45, and 60 g/l) were added into the medium. A single 2 cm plantlet derived from somatic embryo was cultured on a culture tube. Each treatment consisted of 15 plantlets. The cultures were incubated in a culture room with light intensity at 20 mmol/m2/s and temperature at 26 oC. The results show that different types and concentrations of carbohydrate influenced the growth of sago plantlets significantly, but there was no interaction between the two factors. Sucrose was better than other types of carbohydrate, and the concentration of 30 g/l was better than concentrations of 45 or 60 g/l for the growth and vigor of sago plantlets. Medium with a sucrose level at 30 g/l gave the best performance of sago plantlets based on plantlet height, leaf number, biomass fresh weight, stem diameter, and rooting percentage.

Comparison of DNA Extraction Methods for Microbial Community Analysis in Indonesian Tempe Employing Amplified Ribosomal Intergenic Spacer Analysis CECILIA ANNA SEUMAHU; ANTONIUS SUWANTO; IMAN RUSMANA; DEDY DURYADI SOLIHIN
HAYATI Journal of Biosciences Vol. 19 No. 2 (2012): June 2012
Publisher : Bogor Agricultural University, Indonesia

Tempe fermentation involved complex microbial communities which are only revealed partially through culture dependent methods. Culture-independent methods would be potential to unravel this complex microbial fermentation. Appropriate DNA extraction is an essential tool to obtain reliable data from culture independent method. In this study, we employed two commercial DNA extraction methods to find the best one for microbial community characterization employing amplified ribosomal intergenic spacer analysis (ARISA). Our result showed that PowerFood Microbial DNA Isolation Kit-MOBIO (PFMDIK) is an excellent method for microbial DNA extraction from tempe. It gave high quantity and quality of DNA suitable for PCR amplification of 16S-23S rRNA intergenic spacer to yield a diverse and reproducible ARISA profile.

The Impact of Storage Times of Museum Insect Specimens on PCR Success: Case Study on Moth Collections in Indonesia HARI SUTRISNO
HAYATI Journal of Biosciences Vol. 19 No. 2 (2012): June 2012
Publisher : Bogor Agricultural University, Indonesia

Museum specimens are vast repositories of genetic information of interests to biological researchers. Since a new method in DNA extraction, a non destructive method, has been reported to be successful in extracting DNA of museum specimens even fossils without any morphological damages, using museum specimens as resources of genetic information for molecular studies is becoming popular recently. However, the PCR success depends on the quality of the specimens. To evaluate the impact of the storage times of museum specimens on PCR success, we conducted DNA extraction of 14 dry museum specimens of the moths collected from 1992 to 2010 by using a non destructive method. The results showed that the DNA specimens museum were fragmented into various sizes (100-1000 bp) depend on the storage times. On the other hand, fresh specimens which were preserved within absolute ethanol were almost not fragmented. The specimens of < 6 years old (2005-2010) succeed to amplify in 650 bp amplicon but for some specimens of 7 years old (2 of 3 specimens) resulted in a very weak amplification. These specimens, however, were able to amplify strongly in 300 bp amplicon. The results also showed that specimens of 1-19 years old were success to amplify in 100 bp amplicon.

Properties of Folate Binding Protein Purified from Cow’s Milk . SUBANDRATE; DWIRINI RETNO GUNARTI; MOHAMAD SADIKIN
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Folic acid played an important role in the metabolism of the body. To measure the serum folic acid levels could use the folate binding protein (FBP) from cow’s milk with a technique analogous to ELISA. The aims of this study were to identify characteristics of FBP from cow’s milk and binding capacity of FBP to folic acid and to purify FBP from other whey protein passed through DEAE-cellulose chromatography column. Each of DEAE-cellulose peaks was passed in affinity chromatography column. FBP was released from affinity column with sodium acetate buffer pH 3.5. The purity of obtained FBP was demonstrated by a single spot in SDS-PAGE analysis and the estimated molecular weight of FBP was around 31 kDa. Our study indicated that 1 mol FBP bound 1 mol folic acid. Alkylation with iodoacetic acid decreased the binding capacity of FBP which suggested the presence of a–SH or imidazol group in its active site. The importance of disulfide bridge was proven by decreasing of folate binding capacity of FBP after b-mercaptoethanol treatment. In contrary, the folate binding didn need Ca2+ ion, as indicated by EDTA test which gave the same result as control.

Cycad Aulacaspis Scale, a Newly Introduced Insect Pest in Indonesia RANGASWAMY MUNIAPPAN; GILLIAN W. WATSON; GREGORY ALLYN EVANS; AUNU RAUF; NATALIA VON ELLENRIEDER
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Cycad aulacaspis scale (Aulacaspis yasumatsui Takagi (Hemiptera: Diaspididae)) is native to Thailand and Vietnam. Since the early 1990s it has been spreading around the world due to the trade in cycad plants for ornamental use. Infestation by this scale can kill cycads in only a few months. Its accidental introduction to Florida endangered the ornamental cycad-growing industry; and in Guam and Taiwan, endemic cycads (Cycas micronesica and C. taitungensis, respectively) are currently threatened with extinction by cycad aulacaspis scale. In November 2011, an introduced scale was discovered damaging cycads in the Bogor Botanic Garden. Samples from Bogor were taken for identification of the scale, and the material was kept for some time to rear out any insect parasitoids that were present. Both the scale insects and parasitoids were prepared on microscope slides and studied microscopically for authoritative identification. The scale was confirmed as A. yasumatsui. The parasitoid Arrhenophagus chionaspidis Aurivillius (Hymenoptera: Encyrtidae) and the hyperparasitoid Signiphora bifasciata Ashmead (Hymenoptera: Signiphoridae) were identified from the samples. Unless immediate remedial measures are taken, several endemic species of cycad in Indonesia may be endangered by infestation by cycad aulacaspis scale.

Cohabitation Study of the Leaf Monkey and Bornean White-Bearded Gibbons in Gunung Palung National Park, West Kalimantan YANTO SANTOSA; . TAQIUDDIN; ABDUL HARIS MUSTARI; DEDE AULIA RAHMAN
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Diet and habitat overlaps were studied for the leaf monkey (Presbytis rubicunda) and bornean white-bearded gibbons (Hylobates albibarbis) in tropical forest of Cabang Panti Research Station (CPRS), Gunung Palung National Park, West Kalimantan. Systematic data on feeding and ranging behaviour were collected from August 2009 up to February 2010 for the three groups of two sympatric primate species that shared two neighbouring patches. Our results showed that seven types of habitat in CPRS were affected to both primates, particularly in plant utilization for feed and the use of vertical space patterns. If the leaf monkeys were present in the same forest patch, the Bornean white-bearded gibbons showed a reduced within-group dispersal and significantly less foraging time in a given forest patch. This might be due to the bornean white-bearded gibbons were more selective in their diet selection. When fruits were scarce, bornean white-bearded gibbons spent most of their foraging time in many types of forest ecosystem, while leaf monkey foraged within one or two types of forest ecosystem. At this period, diet and habitat overlaps between the two species were low. When the availability of fruits increased, leaf monkeys shifted their foraging range and both species became confined to the forest habitat. Consequently, the overlaps of diets and habitats were increased while the peak was at the end of the fruit season.

Association of Sexual Maturation and Body Size of Arfak Children ELDA IRMA JEANNE JOICE KAWULUR; BAMBANG SURYOBROTO; SRI BUDIARTI; ALEX HARTANA
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Gonad maturation in pubertal girls and boys is accompanied with somatic growth spurt, changes in quantity and distribution of body fat (BF), development of secondary sex characters, and relevant physiological events. Menarche (first event of menstruation) and spermarche (first event of nocturnal sperm emission) are usually used as indicators of gonad maturation. We found that median age at menarche of Arfak girls in Manokwari, West Papua is 12.2 years, while median age at spermarche of boys is 13.6 years. A possible factor causing young age at menarche is due to adaptation to unstable environmental conditions because of high risk of mortality by malaria disease during childhood. The events of menarche and spermarche achieved one year after the peak body height (BH) velocity, and just before or at the same time with the time of maximum growth rate of body weight (BW), body mass index (BMI), and BF. The average BMI of Arfak girls was big at 21.9 kg/m2 at the time of their menarche. Bigger average BMI might be caused by prepubertal slowing down of BH growth compare to growth of BW whichis still increasing. Girls accumulate BF before puberty to be used as an energy reserve for the occurrence of menarche. At the time of development of secondary sexual characters girls use the fat reserve so it decline sharply after puberty. In boys, growth rate of BF was stopped at 11 years old, and then growing negatively presumably because boys use fat mass for the occurence of spemarche. BF growth rate reached the lowest point at the age 16 years old, and then increase linearly with age through adolescence until adulthood at age 23 years old.

Phage FR38 Treatment on Sprague Dawley Rat Inferred from Blood Parameters and Organ Systems DEWI SARTIKA; SRI BUDIARTI; MIRNAWATI SUDARWANTO
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

The ability of phage FR38 to lysis indigenous Salmonella P38 from feces of diarrheal patient has been studied. However, effects of phage FR38 on organ system were not revealed as yet. This study was conducted to observe the effect of phage FR38 on blood chemistry, kidney functions, and liver functions. Twelve Sprague-Dawley rats were used as a model for this study that were divided into two groups; (i) control and (ii) treated group with phage FR38. For treated phage group, each rat was administered by 5 ml/kg bw of 1.59·107 pfu/ml of phage intragastric. The blood parameters were analysed on day 16. The results revealed that body and organs weight, erythrocyte, hematocrit, hemoglobin, leukocyte, total protein, creatinine, SGOT, and SGPT of phage treatment rats were not significantly different with the control rats on day 16 (P > 0.05). Therefore, this study showed was no effect of phage FR38 on body weight, blood chemistry, kidney and liver functions of the rat (P > 0.05).

Page 25 of 108 | Total Record : 1077

23 24 25 26 27

Filter by Year

2005 2026

Filter By Issues

All Issue Vol. 33 No. 2 (2026): March 2026 Vol. 33 No. 1 (2026): January 2026 Vol. 32 No. 6 (2025): November 2025 Vol. 32 No. 5 (2025): September 2025 Vol. 32 No. 4 (2025): July 2025 Vol. 32 No. 3 (2025): May 2025 Vol. 32 No. 2 (2025): March 2025 Vol. 32 No. 1 (2025): January 2025 Vol. 31 No. 6 (2024): November 2024 Vol. 31 No. 5 (2024): September 2024 Vol. 31 No. 4 (2024): July 2024 Vol. 31 No. 3 (2024): May 2024 Vol. 31 No. 2 (2024): March 2024 Vol. 31 No. 1 (2024): January 2024 Vol. 30 No. 6 (2023): November 2023 Vol. 30 No. 5 (2023): September 2023 Vol. 30 No. 4 (2023): July 2023 Vol. 30 No. 3 (2023): May 2023 Vol. 30 No. 2 (2023): March 2023 Vol. 30 No. 1 (2023): January 2023 Vol. 29 No. 6 (2022): November 2022 Vol. 29 No. 5 (2022): September 2022 Vol. 29 No. 4 (2022): July 2022 Vol. 29 No. 3 (2022): May 2022 Vol. 29 No. 2 (2022): March 2022 Vol. 29 No. 1 (2022): January 2022 Vol. 28 No. 4 (2021): October 2021 Vol. 28 No. 3 (2021): July 2021 Vol. 28 No. 2 (2021): April 2021 Vol. 28 No. 1 (2021): January 2021 Vol. 27 No. 4 (2020): October 2020 Vol. 27 No. 3 (2020): July 2020 Vol. 27 No. 2 (2020): April 2020 Vol. 27 No. 1 (2020): January 2020 Vol. 26 No. 4 (2019): October 2019 Vol. 26 No. 3 (2019): July 2019 Vol. 26 No. 2 (2019): April 2019 Vol. 26 No. 1 (2019): January 2019 Vol. 25 No. 4 (2018): October 2018 Vol. 25 No. 3 (2018): July 2018 Vol. 25 No. 2 (2018): April 2018 Vol. 25 No. 1 (2018): January 2018 Vol. 24 No. 4 (2017): October 2017 Vol. 24 No. 3 (2017): July 2017 Vol. 24 No. 2 (2017): April 2017 Vol. 24 No. 1 (2017): January 2017 Vol. 23 No. 4 (2016): October 2016 Vol. 23 No. 3 (2016): July 2016 Vol. 23 No. 2 (2016): April 2016 Vol. 23 No. 1 (2016): January 2016 Vol. 22 No. 4 (2015): October 2015 Vol. 22 No. 3 (2015): July 2015 Vol. 22 No. 2 (2015): April 2015 Vol. 22 No. 1 (2015): January 2015 Vol. 21 No. 4 (2014): December 2014 Vol. 21 No. 3 (2014): September 2014 Vol. 21 No. 2 (2014): June 2014 Vol. 21 No. 1 (2014): March 2014 Vol. 20 No. 4 (2013): December 2013 Vol. 20 No. 3 (2013): September 2013 Vol. 20 No. 2 (2013): June 2013 Vol. 20 No. 1 (2013): March 2013 Vol. 19 No. 4 (2012): December 2012 Vol. 19 No. 3 (2012): September 2012 Vol. 19 No. 2 (2012): June 2012 Vol. 19 No. 1 (2012): March 2012 Vol. 18 No. 4 (2011): December 2011 Vol. 18 No. 3 (2011): September 2011 Vol. 18 No. 2 (2011): June 2011 Vol. 18 No. 1 (2011): March 2011 Vol. 17 No. 4 (2010): December 2010 Vol. 17 No. 3 (2010): September 2010 Vol. 17 No. 2 (2010): June 2010 Vol. 17 No. 1 (2010): March 2010 Vol. 16 No. 4 (2009): December 2009 Vol. 16 No. 3 (2009): September 2009 Vol. 16 No. 2 (2009): June 2009 Vol. 16 No. 1 (2009): March 2009 Vol. 15 No. 4 (2008): December 2008 Vol. 15 No. 3 (2008): September 2008 Vol. 15 No. 2 (2008): June 2008 Vol. 15 No. 1 (2008): March 2008 Vol. 14 No. 4 (2007): December 2007 Vol. 14 No. 3 (2007): September 2007 Vol. 14 No. 2 (2007): June 2007 Vol. 14 No. 1 (2007): March 2007 Vol. 13 No. 4 (2006): December 2006 Vol. 13 No. 3 (2006): September 2006 Vol. 13 No. 2 (2006): June 2006 Vol. 13 No. 1 (2006): March 2006 Vol. 12 No. 4 (2005): December 2005 Vol. 12 No. 3 (2005): September 2005 Vol. 12 No. 2 (2005): June 2005 Vol. 12 No. 1 (2005): March 2005 More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
hayati_j_biosci@cbn.net.id

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official hayati_j_biosci@cbn.net.id

Editorial Address -

Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
hayati_j_biosci@cbn.net.id

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA