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HAYATI Journal of Biosciences
ISSN : 19783019     EISSN : 20864094     DOI : -
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
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Articles 1,091 Documents
Soybean Seeds (Glycine max L.) Extract Against Cytokine Storm in ARDS Rat Model through Inhibiting Inflammation Marker Didik Priyandoko; Wahyu Widowati; Ervi Afifah; Ika Adhani Sholihah; Cintani Dewi Wahyuni; Cahyaning Riski Wijayanti; Rizal Rizal
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.779-788

Abstract

Several studies have suggested that "cytokine storms" are significant causes of the severity of COVID-19. Controlling and inhibiting the cytokine storm in COVID-19 could prevent the spread of COVID-19 and saves patient lives. Soybean (Glycine max L.) is known to have various biological activities. This study aims to examine bioactive compounds in SSE and the effect of SSE on the ARDS rats model. A total of 25 Sprague Dawley Lipopolysaccharide-induced rats were used. Determination of serum IL-1β, IL-12, and lung TNF-α levels was performed by ELISA method. NF-κB and IFN-γ expression were determined by the qRTPCR method. IL-6 expressions were analyzed by immunohistochemistry assay. The bleeding, inflammation, and alveolus collapse score were analyzed using the HE staining method. The results showed that SSE could decrease the level of IL-1β, IL-12, TNF-α, IL-6, NF-kB, and IFN-γ and improve the bleeding, inflammation, and alveolus score in the lung. SSE could decrease the pro-inflammatory cytokines and improve lung condition in ARDS rats model.
Diet Composition and Overlap of Two Mesocarnivores, Leopard Cat (Prionailurus bengalensis) and Javan Mongoose (Urva javanica), in Java, Indonesia Sena Adi Subrata; Ulfah Darmayani Permatasari
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.596-605

Abstract

Dietary study of carnivores has garnered a great deal of attention for a very long time since it provides information for comprehending ecological processes and tracking the economic effects of animals on food production. Few studies reported alternative prey that is detrimental to food production, such as rodents and insects, in addition to the numerous studies on livestock predation. Therefore, this study aims to provide information on the diet composition and overlap of two mesocarnivore species, Javan mongoose (Urva javanica) and leopard cat (Prionailurus bengalensis), in Java, Indonesia. The macroscopic observation technique was applied for identifying food remnants found in the 90 feces of the mongoose and 100 feces of the cat collected in 2018. Twenty three and 21 taxa of prey were found in the leopard and Javan mongoose feces. Rodentia was the most frequent taxa found in the feces of both species, followed by Galliformes. Insects comprised the majority of diet items but at low frequencies. Two mesocarnivores were specialists (Levin’s index of leopard cat: 0.08; Javan mongoose: 0.26) with fairly high diet overlap (Pianka’s index = 0.78). The results provided compelling evidence of the positive and negative impacts of mesocarnivores on food production in Java.
The Impacts of Pieh Marine Protected Area on Reef Fish Resources In Its Adjacent Areas Reny Puspasari; Budi Nugraha; Puput Fitri Rachmawati; Rita Rachmawati; Dian Oktaviani; Andriyanto Hanif
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.662-669

Abstract

Reef fish have a high economic value. Excessive fishing causes degradation of reef fish resources, as has occurred in the West Sumatera waters. Marine protected areas (MPA) offer one of the better management strategies in support of the restoration of the overexploited fish resources by protecting fish habitat and limiting exploitation. This study aims to analyze the impacts of the establishment of MPA on reef fish resources in its adjacent areas. The research was carried out in Pieh MPA and its adjacent areas located in the West Sumatra waters. Fishing experiments were conducted inside the core zone of the MPA and the catch was compared to similar experiments outside MPA. The result showed that the catch per unit effort (CPUE) of reef fishes in the core zone of MPA was 2.47 times higher than that of in the outside of MPA. However, the size composition was not significantly different between those two areas, except for Lutjanus russellii, which shows that the maximum length inside MPA was about 2.5 times higher. The length and weight of Epinephelus areolatus were analyzed to determine the biological condition of the grouper caught in both areas. The length-weight analysis shows that 86.67% of E. areolatus were matured. Nevertheless, the length of maturity in the MPA is smaller, indicating there is the impact of epreviously fishing pressure on E. areolatus. Research results showed that the MPA is double in fish abundance.
Activity Budget and Diet in Sumatran Orangutan (Pongo abelii) at Soraya Research Station, Aceh Mahyana; Kanthi Arum Widayati; Puji Rianti
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.653-661

Abstract

Geographical and environmental factors influence the food availability and diet of orangutans. Moreover, tree phenology affects productivity and food availability in orangutan habitats. As a result, orangutans must be able to adapt to their food availability in the wild. This study aims to analyse the daily activity patterns and eating behaviour of the Sumatran orangutan (Pongo abelii) and their relationship to food availability through the fruit availability index (FAI) and phenology at Soraya Research Station (SRS), Aceh. From the 648 hours of observation on five orangutans' daily activities, we found that resting is the activity with the highest percentage (48%), followed by feeding (22.5%) and moving (21.8%). The high percentage of resting activity might correlate with its low FAI in SRS (1.01%). The resting activity limits orangutans' energy expenditure. Furthermore, barks, fruits, and other plants (including lianas, ferns, and epiphytes) were the main type of diet of Sumatran orangutans in SRS with a frequency of 40.6%, 28.9%, and 15.6%, respectively. The high bark consumption by the orangutans acts as their fallback diet at SRS and is thought to be a form of adaptation to the low fruit availability. This study showed the orangutan adaptation to low fruit availability in SRS through their daily activity and diet patterns.
Bird Sounds Psychological Restorative Effect on The Visitors of Bogor Botanical Garden Yuriko Asahiro; Arzyana Sunkar; Jarwadi Budi Hernowo
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.692-700

Abstract

Stress can be defined as psychological and physical tension caused by physical, emotional, social, economic, and occupational, events, or experiences that are difficult to manage or endure. Bird sounds can be a potential solution to reduce stress levels. This study aimed to analyze the effect of bird sounds as a stress restorative on Bogor Botanical Garden visitors. A survey of 100 visitors of Bogor Botanical Garden was conducted based on the Perceived Stress Scale and Perceived Restorativeness Soundscape Scale. Data on respondent characteristics were analyzed using descriptive analysis, the relationship between stress levels and perceived stress restorative effect of bird sound was analyzed using Pearson correlation analysis. The analysis showed that 78% of respondents had moderate to high perceived stress levels and 98% of respondents rated the degree of restoration from bird sounds to be moderate to high. The correlation analysis revealed a negative relationship between the stress level and bird sounds' perceived restorative effect. The older the respondents and the more frequently they visited Bogor Botanical Garden, the higher the perceived stress restorative effect from bird sound.
Development of Phenol Red Colorimetric RT-LAMP Assay in High-Buffered SARS-CoV-2 Sample Putera Rakhmat; Uus Saepuloh; Huda Shalahuddin Darusman
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.621-631

Abstract

Colorimetric RT-LAMP Assay is a diagnostic method that has attracted much attention because of its rapidity, simplicity, and accuracy compared to other disease diagnosis methods. Despite having many advantages, the RT-LAMP Colorimetric Assay has disadvantages, especially for kits that use phenol red as an indicator. The disadvantage derives from the input RNA/DNA samples containing high buffer levels, which causes no color change and false-negative results. This study aimed to develop and optimize the colorimetric RT-LAMP method on high-buffered SARS-CoV-2 RNA samples. We found that a temperature of 69°C for 50 minutes with the addition of post-treatment in the form of heating at 80°C for 10 minutes is an optimal condition for high-buffered SARS-CoV-2 samples. The condition proved effective in changing the result's color from red (negative) to yellow (positive). We also classified the analysis results based on the correlation between the Cycle threshold (Ct) value of SARS-CoV-2 viruses and the Optical Density (OD) value, which was quantified using a spectrophotometer at 415 nm (with a correlation value of -0.9084), where yellow color indicated Ct below 20, amber color indicated Ct between 20 and 30, orange color indicated Ct between 30 and 35, and red indicated Ct more than 35 (negative). In conclusion, this study successfully detects the SARS-CoV-2 virus in high-buffered samples using Phenol Red Colorimetric RT-LAMP Assay, with a sensitivity of 85% for Ct Cutoff 40.
Age and Growth Estimation of Bigeye Tuna, Thunnus obesus (Lowe, 1839) in the Eastern Indian Ocean Deduced from Otolith Microstructure Arief Wujdi; Suciadi Catur Nugroho; Raymon Rahmanov Zedta; Hety Hartaty; Masayu Rahmia Anwar Putri; Fathur Rochman; Prawira Atmaja Rintar Pandapotan Tampubolon; Roy Kurniawan; Yesika Nanda Pramurdya; Bram Setyadji
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.670-681

Abstract

Age-based stock assessment of globally important species, like bigeye tuna Thunnus obesus (Lowe, 1839), is urgently required to develop appropriate management plans for fisheries sustainability. This study aimed to estimate age and growth parameters using annual increments count of sectioned-otoliths, which effectively employed for age determination of tunas. Samples were collected from Indonesian tuna longline catch landed in the Port of Benoa from March until December 2017. Multi-models were employed to obtain robust growth parameters, and Akaike Information Criterion (AIC) was relied for the best fit model selection among those evaluated. Growth band formation was validated using marginal analysis (MI), indicating that one ring is deposited yearly and slow growth peaked during July (Australian winter). The von Bertalanffy growth model was selected as the best model fitting growth parameters from raw age data, as expressed as Lt = 183.49 (1-e-0.134(t+2.991)). Large variances in growth were detected at the same age, with the oldest age of 15. Over 50% of fish in the Indonesian catch were <5 years old, that may have implications for fisheries sustainability.
Population Management of Samia cynthia ricini Boisduval (Lepidoptera: Saturniidae) by Eggs Refrigeration and Its Effect on Hatchability, Development, and Fecundity Ihsan Nurkomar; Dina Wahyu Trisnawati; Narisara Piyasaengthong; Putri Nafisah Kirana
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.606-611

Abstract

Egg viability, hatchability, and development are significant factors in the natural silk industry. Life cycle modification is an important aspect that must be considered to perform effective and efficient mass-rearing and quality control. This study aimed to improve the mass rearing efficiency of Samia chintya ricini through a life cycle modification technique by refrigeration. The study was conducted by storing S. c. ricini eggs in the refrigerator at a temperature of 5°C for one to seven days. Observations were made on the hatchability, development time, cocoon shell weight, and adult female fecundity. The results showed that the egg refrigeration period affected the hatchability of the eggs. A more extended period of egg refrigeration significantly decreased hatchability. The eggs could develop into adult insects after being stored for one to five days. Meanwhile, none of the eggs hatched successfully after going through a cold storage period of six and seven days. Furthermore, the egg refrigeration period affected the development time of the egg, larvae, pupae, and adult. A more extended period of egg refrigeration results in slower egg and larval development time. But a faster cocoon development time. The refrigeration period did not significantly affect the cocoon shell weight. However, it was affecting adult female fecundity. Total fecundity decreased as the refrigeration period increased with different daily oviposition patterns. This study implies that when the egg population is excessive, the life cycle of S. c. ricini can be delayed for 1-5 days. This method can be implied as a simple life cycle modification technique for eri silkworm cultivation.
Morphological Alterations of Fish Erythrocytes as Their Response to Environmental Conditions D.H. Quyet; P.T. Dung
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.711-715

Abstract

Fish bodies respond to changes in environmental conditions in various ways and degrees. Seven fish species were collected from three study locations, including two nearshore sites (Ha Tinh province and Nha Trang city, Khanh Hoa province) and one offshore site (Toc Tan island, Khanh Hoa province) to evaluate alterations in erythrocyte morphology under certain environmental conditions. Heavy metal concentrations at all study sites were analyzed and met the acceptable technical criteria for each site. Fish blood samples were taken from the tail veins, anticoagulated with heparin, and stained with Giemsa stain. The blood cell morphological study was performed using an optical microscope. The results revealed two types of morphological abnormalities of fish erythrocytes, including nuclear deformation and nuclear-matter distribution.
A Combination of Anadara nodifera Shell and Milkfish Thorns Powder Effectively Promote Springiness Index, Serum Testosterone, and Breast Muscle Testosterone in Bangkok Rooster Akhmad Abror As Sidiqi; Claude Mona Airin; Sarmin Sarmin; Pudji Astuti
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.701-710

Abstract

Aromatase blocker effectively promotes testosterone, which improves muscle performance and protein synthesis. Considerable muscle strength in Bangkok rooster is the outcome of testosterone activity. Clamshell contains aromatase blocker substances, while fishbone possesses a high amount of protein. This research aimed to evaluate the clamshell and fishbone combination potency towards testosterone levels and muscle performance in Bangkok roosters. This study employed 18 Bangkok roosters under 35 days of treatment. The rooster groups consisted of control (P0, n = 6); clamshell-fishbone (P1, n = 6): clamshell (6.6 gram/day) + fishbone (3.3 gram/day); fishbone (P2, n = 6): fishbone (3.3 gram/day). The blood was collected once every seven days. Enzyme immunoassay showed the highest serum and muscle testosterone levels in P1 (p<0.05). Immunohistochemistry presented the most and broadest myofiber and the highest proliferation activity in P1 (p<0.05). The texture analysis showed the topmost springiness index in P1 (p<0.05). Aromatase blockers in clamshell and protein in fishbone improve testosterone and muscle performance in Bangkok roosters.

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