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INDONESIA
HAYATI Journal of Biosciences
Published by Institut Pertanian Bogor
ISSN : 19783019     EISSN : 20864094     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
Arjuna Subject : -
Articles 1,091 Documents
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Morpho-Physiology of Mulberry (Morus sp.) Plant on Salinity Stress Tolerance Yasinta Ratna Esti Wulandari; Yohana Caecilia Sulistyaningsih; Agik Suprayogi; Min Rahminiwati; Triadiati Triadiati
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.682-691

Abstract

The accession of mulberry (Morus sp.) with salinity tolerance unavailable in Indonesia is needed to overcome the problem of marginal land, especially in the saline areas of the country. Therefore, this research aims to determine the accession of salinity tolerance of mulberry from 6 origins in Indonesia based on growth and physiological parameters. The method used was a factorial randomized block design with the first treatment of stem cutting-derived mulberry accession being M1, M2, M3, M4, M5, M6, and M7, while the second was the concentration of NaCl at 0 mM, 40 mM, 60 mM, and 80 mM. The results showed that M2 accession from Bogor was categorized as tolerance to high salt stress, and M1 was classified as sensitive accession. Increasing salt concentration causes a decrease in growth parameters. It also decreased physiological parameters such as leaf and media relative water content while increasing dissolved sugars. Genotypic scores indicate salinity tolerance and the potential number of leaves, while Na+ content and leaf necrosis percentage showed sodium toxicity in the leaf. Therefore, genotypic score, Na+/K+ ratio, and leaf necrosis percentage are the best parameters to select and identify mulberry accession with high salt stress tolerance.
UHPLC-Q-Orbitrap HRMS-based Untargeted Metabolomics of Sida rhombifolia Leaves and Stem Extracts Alfi Hudatul Karomah; Mohamad Rafi; Dewi Anggraini Septaningsih; Auliya Ilmiawati; Utami Dyah Syafitri; Nanik Siti Aminah; Muhamad Insanu; Abdul Rohman
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.770-778

Abstract

Sida rhombifolia, also known as sidaguri in Indonesia, is a medicinal plant commonly used as a herbal medicine because of its metabolite and biological activities. One of the several factors that affect plant metabolite composition and concentration is the use of plant parts. In this study, the experiment aimed to identify the metabolite profile in the leaves and stem extracts of S. rhombifolia using UHPLC-Q-Orbitrap HRMS-based untargeted metabolomics. The samples were distinguished by principal component analysis (PCA). Extraction of metabolites was conducted by sonication for approximately 30 min with 70% ethanol as the extraction solvent; 28 metabolites were identified. Seven metabolites were identified only in the leaves, three were identified only in the stems, and 18 other metabolites were identified in both the leaves and stems. These metabolites were categorized as flavonoids, triterpenoids, alkaloids, coumarins, phenolic aldehydes, phenolic acids, ecdysteroids, fatty acids, and monoterpene lactones. Based on the classification results, PCA grouped the leaves and stem extracts of S. rhombifolia using the peak area variables of the identified metabolites.
Quercetin-Containing Extract from Clove Syzygium aromaticum L. Endophytic Bacteria, Fictibacillus phosphorivorans P1U2, Exhibits Antimutagenic Activity in Yeast Saccharomyces cerevisiae Nadila Fany Ashafani; Rika Indri Astuti; Sri Listiyowati; Irmanida Batubara
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.734-742

Abstract

Metabolites from clove (Syzygium aromaticum L.) have been extensively studied for various pharmacological uses, including quercetin. Endophytic bacteria isolated from plant tissues can produce similar secondary metabolites as their host plants. However, little is known about the antioxidant activity of the endophytic bacterial extract from clove leaves. Amongst the potential bacterial groups, the genera of Bacillus have been widely studied as a source of antioxidants. Thus, this study, we examined the potential antioxidant properties of Bacillus sp., isolated from clove leaves. We have successfully isolated ten endophytic bacteria, of which only one isolate (P1U2) was found non-pathogenic based on a hemolytic assay. Based on the 16S rRNA sequence, P1U2 shared the highest similarity to Fictibacillus phosphorivorans. The extract of extracellular metabolites of P1U2 contains quercetin compounds, based on LC-MS analysis. The results of the antioxidant activity test using the DPPH assay showed that the P1U2 extract had an IC50 value of 161 mg/ml. The supplementation of the extract on culture media reduced the ability of S. cerevisiae to decolorize mutagen malachite green and increased cell viability. Furthermore, the addition of 161 ppm extract was able to counteract UV mutagens effects of UV exposure and toxicity of malachite green, significantly increasing the growth of S. cerevisiae. Suggesting the bioactivity of the extract in combating mutagens. To the best of our knowledge, this is the first study to report a quercetin-rich extract derived from endophytic bacteria, F. phosphorivorans. Thus, further study is required to optimize the synthesis of quercetin from isolate P1U2.
Phylogeographic Insights of Five Co-Habiting Grouper Species in The Indo-Malaya Archipelago Nur Fadli; Adrian Damora; Zainal A. Muchlisin; Irma Dewiyanti; Mutia Ramadhaniaty; Nanda Muhammad Razi; Jamsari Amirul Firdaus Jamaluddin; Edison D. Macusi; Mohd Nor Siti-Azizah
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.743-756

Abstract

Understanding the patterns of genetic diversity of species and precise stock identification are important in fisheries conservation management. However, studies on genetic diversity, connectivity, population structure, and gene flow of groupers within the Indo-Malaya Archipelago (IMA) waters are limited. The objective of the present study was to examine the phylogeographic patterns of the family Epinephelidae in IMA waters based on a parallel study of five selected species utilizing the mitochondrial COI. The grouper species were: areolate grouper (Epinephelus areolatus), blacktip grouper (Epinephelus fasciatus), six-bar grouper (Epinephelus sexfasciatus), blue-lined hind (Cephalopholis formosa), and white-edged lyretail (Variola albimarginata). Specimens were obtained from fish landing sites and fish markets from 23 locations throughout the IMA waters. This study showed genetic structuring for two species (Epinephelus areolatus and Variola albimarginata) but genetic homogeneity for the other three investigated species across IMA. Various geological and demographic histories, local and regional oceanographic features, and biological characteristics are hypothesized to shape the present genetic pattern of each species across the IMA waters. The establishment of effective international cooperation is encouraged to manage grouper species stocks in this region.
Chemical Composition, Antioxidant, Antibacterial, Antibiofilm, and Cytotoxic Activities of Robusta Coffee Extract (Coffea canephora) Erma Suryanti; Dwi Retnowati; Muhammad Eka Prastya; Novita Ariani; Indri Yati; Vera Permatasari; Tjandrawati Mozef; Indah D. Dewijanti; Apriza Yuswan; Muhammad Asril; Elisa Nurma Riana; Irmanida Batubara
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.632-642

Abstract

Coffee extracts are a rich source of potential compounds with numerous biological activities. This study aimed to investigate the potential of chemical compounds derived from robusta coffee extract (Coffea canephora) and its dominant compound, caffeine, to scavenge free radicals, inhibit bacterial growth and biofilm formation, and cytotoxic properties in human breast adenocarcinoma cell line. Chemical constituents of coffee extract were analysed quantitively for total phenolic, flavonoid, and alkaloid contents, along with Liquid Chromatography Quadrupole-Mass Spectrometry (LC/MS–MS) analysis. Antioxidant activity was measured by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis; 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods. Antibacterial pair with antibiofilm properties against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus were also determined, as well as cytotoxic activity on the MCF7 cell line model. The LC/MS–MS analysis of coffee extracts revealed high levels of caffeine; thus, the caffeine standard is used in all subsequent assays. Notably, robusta coffee extract showed remarkable antioxidant activity and selective inhibition of the growth against gram-positive and negative bacteria, along with the best inhibition of P. aeruginosa biofilm. However, compared with the caffeine standard, robusta coffee extract had lower cytotoxic activity and different bacterial targets in antibacterial and antibiofilm properties. Our results indicate that Robusta coffee extract is potentially a functional food due to its high alkaloid, phenolic, and flavonoid content and antioxidant activity, besides being used for natural compounds against bacterial infections.
The Patterns of Restriction Fragment of Several Enzymes to Distinguish Toxoplasma gondii Isolates Virulent and Avirulent Strains using GRA1 and GRA7 Genetic Marker Fitrine Ekawasti; Eko Setyo Purwanto; Farlin Nepho; Dyah Ayu Kurniawati; Didik Tulus Subekti; Rini Damayanti; Siti Sa’diah; Umi Cahyaningsih
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.725-733

Abstract

Toxoplasma gondii pathogenicity depends on the type derived from a clonal population. A genetic analysis of the locus has been carried out to determine the different genotypes of T. gondii (strain types I, II, and III) that are associated with human toxoplasmosis. The several genotypes of T. gondii (strain types I, II, and III) that are linked to human toxoplasmosis have been identified through genetic study of the locus. In this investigation, PCR-RFLP was found to be a useful, and simple method genotypic characterization. The objective of this study was to genotyping characterize T. gondii RH and BEV strains isolates by PCR-RFLP using several restriction enzymes. T. gondii tachyzoite DNA was extracted and amplified by PCR using dense granule genetic markers (GRA1 and GRA7) designed with Primer3plus. The amplification were digested using the restriction enzymes. The PCR-RFLP amplified dense granule products was used to classify strains into two genotypes of T. gondii (virulent and avirulent). The results demonstrated that the RFLP patterns of the GRA1 and GRA7 gene area digested by DdeI, MvaI, HinfI, RsaI, and Sau96I enzymes can be used to identify virulent or avirulent strains of T. gondii. Toxoplasma gondii RH and BEV strain produced different digestion product which can be used to distinguished the strains.
First Records of Male and Swollen Females of Two Species of Achlysiella (Nematoda: Pratylenchidae) Abdul Gafur; Mike Hodda
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.757-769

Abstract

Males and swollen mature females of Achlysiella trilineata and Achlysiella magniglans were discovered for the first time in Australia. These life stages confirmed that both species show well-marked sexual dimorphism in the anterior region of the body, with the males having reduced cephalic frameworks, stylets, and oesophagi. Males of A. magniglans displayed an extreme reduction in the style such that only the cone could be seen, and the rest of the esophagus was barely visible. In both species, the swollen females had postmedian vulvae, and the anterior branch of the ovary was longer than the posterior branch. In A. magniglans, the disparity in length was more pronounced, and the vulva was more posterior. The finding of swollen females confirmed the classification of both species in Achlysiella rather than Radopholus. Vermiform females of both species have large dorsal oesophageal glands and short ovaries. Justifiably, other species of Radopholus with these latter features (R. brevicaudatus, R. capitatus, and R. vacuus) should also be moved to Achlysiella.
Development of Secondary Forest Succession Based on Estimation of Forest Carbon Stocks Ten Years Post-Merapi Volcano Eruption Inggita Utami; Anjar Dimara Sakti; Febriant Isabella Yusuf; Fahmiatul Husna; Denni Susanto
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.834-842

Abstract

The eruption of the most active volcano in Indonesia, Mount Merapi, has resulted in a very dynamic landscape as a form of ecosystem succession. This study aimed to analyze the carbon stock and vegetation composition in the secondary forest with variations in the level of disturbance after the 2010 eruption of Mount Merapi. Data collection was carried out in January 2020, in which biomass, necromass, and soil organic carbon data were taken in plot transect and Geographic Information Systems carbon stock products compared field data. The results showed that secondary forests at stations A and B with low and medium disturbance levels had carbon stocks in the high category. In contrast, station C at Cangkringan Resort, with high disturbance levels, still had carbon stock with a low category. Furthermore, the comparison of 2020 field data with GIS carbon stock products concluded a similar pattern between carbon stock from aboveground biomass WHRC 2010 and belowground biomass NASA 2010. This research concludes that the disturbance affected forest carbon stocks ten years after the 2010 Merapi eruption. Therefore, monitoring the vegetation community needs to be carried out annually through a combination of spatial and direct checks in the field.
Taxonomic Profiling of Microorganisms Inhabiting Two Solar Salterns that Produce High- and Low-Quality Salts Ekowati Chasanah; Asri Pratitis; Rini Susilowati; Agus Heri Purnomo; Hedi I. Januar; Agustinus R. Uria
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.957-984

Abstract

The contribution of halophilic microorganisms to the quality of salts produced in solar salterns has recently been recognized but not clearly understood. Using metagenomic 16S rRNA gene sequencing approach, we showed the microbial composition difference between the Tuban crystallization pond (CP-Tuban) that produces low-quality salt and CP-Sampang as a representative solar saltern that yields high-quality salt. Dominant classes in both traditional salterns were represented by γ-proteobacteria and halobacteria that occurred at higher prevalence in CP-Sampang. Microbial taxa, including beneficial genera, in CP-Sampang were more diverse and abundant compared to CP-Tuban. Among 180 genus-level OTUs identified in CP-Sampang, 127 of them were considered unique due to their absence in CP-Tuban. Higher levels of dissolved oxygen (DO) and nutrient (phosphate, nitrate, and ammonia) in the seawater reservoir (SR) of Sampang may contribute to more diverse phytoplankton genera, which could support the growth of beneficial heterotrophic microbes that positively affect the salt quality of the CP-Sampang. Low number of Dunaleilla sp. in both CPs do not seem to influence the quality of salts produced. The outcome of these comparative studies provides new insights into the contribution of diverse microbial taxa in correlation with physico-chemical parameters and phytoplankton communities to the high quality of salts produced in traditional solar salterns. The presence of beneficial genera in the enriched microbial cultures could provide an important basis for further applications, such as improving the quality of salt produced and producing unique compounds and enzymes.
Optimization of Biomass and Secondary Metabolite Production in Gynura procumbens (Lour.) Merr. Adventitious Roots Culture by Using the Method of Subculture and Fed-batch Cultivation in a Bioreactor Dannis Yuda Kusuma; Alfinda Novi Kristanti; Anjar Tri Wibowo; Boon Chin Tan; Yosephine Sri Wulan Manuhara
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.797-807

Abstract

The valuable extract of bioactive compounds from Gynura procumbens has been widely manufactured into various health products. The demand for these compounds is continuously increasing, but production through conventional farming methods is insufficient due to limited agricultural land and environmental stresses. An alternative to producing plant biomass is in vitro cultivation methods. This method requires less space and enables biomass propagation in a controlled condition that can facilitate stable and efficient production of plant secondary metabolites. This study evaluated the effect of inoculum subculture periods and culture methods on G. procumbens biomass and secondary metabolite production in a bioreactor. The 3-L airlift balloon type-bubble bioreactors was modified in this study to adopt the treatment of 1st-5th subculture periods and fed- and batch-cultivation strategies. We found the G. procumbens adventitious root culture was optimally derived from the 1st subculture produced biomass of 148.02±1.45 g FW and 8.59±0.12 g DW, and TPC (14.48±1.08 mg GAE/g DW) and TFC (116.89±0.44 mg KE/g DW and 33.97±0.13 mg QE/g DW). Additionally, the fed method after 28 days of culture using double distilled water replenishment improved adventitious root biomass (213.75±35.00 g FW and 11.21±0.18 g DW), while nutrient replenishment improved TFC (52.14±0.44 mg KE/g DW and 14.54±0.13 mg QE/g DW). These results can be used to optimize the cultivation of G. procumbens adventitious roots in a large-scale bioreactor.

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