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INDONESIA
MEDIA PETERNAKAN - Journal of Animal Science and Technology
Published by Institut Pertanian Bogor
ISSN : -     EISSN : -     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : -
Articles 9 Documents
 1 
Search results for , issue "Vol. 32 No. 2 (2009): Media Peternakan" : 9 Documents clear
Perubahan Malonaldehida Hati, Bobot Relatif Bursa Fabricius dan Rasio Heterofil/Limfosit (H/L) Ayam Broiler yang Diberi Cekaman Panas E Kusnadi
Media Peternakan Vol. 32 No. 2 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The objective of the research was to determine the influences of heat stress and temperature-exposed time on liver malonaldehyde (MDA), relative weight of bursa fabricius and heterophil/lymfocite ratio of broilers. The research used 140 male broilers of 2 weeks of age. The treatments consisted of two factors. The first factor was five treatments: S1A (28.55?1.53 oC) with ad libitum of feeding, S1BT1 (S1 with pair feeding as S2A), S1BT2 (S1 with pair feeding as S3A), S2A (31.07?1.29 oC) with ad libitum of feeding and S3A (33.50?1.17 oC) with ad libitum of feeding. The second factor was five of temperature-exposed times (0, 4, 8, 16, and 32 days after treatments). Variables measured were liver MDA, relative weight of bursa fabricius and ratio of heterophil/lymphocite (H/L). The experiment used a completely randomized design in split plot (5x5), with 4 replications. The result indicated that liver MDA, relative weight of bursa fabricius and H/L ratio of S2 and S3 were higher than those of S1A, S1BT1 and S1BT2. H/L ratio of 4, 8, 16, and 32 days of temperature-exposed times were higher than that of 0 day. It was concluded that heat stress increased the liver MDA and H/L ratio, but decreased the relative weight of bursa fabricius. The temperature-exposed time increased the H/L ratio, but it did not affect the liver MDA and relative weight of bursa fabricius of broilers. Key words: heat stress, malonaldehyde, heterophil/lymphocite ratio, bursa fabricius, broiler
Kualitas Semen Cair Asal Epididimis Kerbau Belang dalam Bahan Pengencer Andromed yang Mendapat Penambahan Sukrosa M Surachman; . Herdis; . Yulnawati; M Rizal; H Maheshwari
Media Peternakan Vol. 32 No. 2 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The aim of this research was to observe the quality of spotted buffalo epididymal sperm after storage at 4 °C as liquid semen for 12 and 24 hours in andromed containing sucrose 0.2% and 0.4%. Sperm was collected from epididymal tissues by combination of slicing and pressing methods. The parameters observed were the percentage of progressive motility and live sperm. The results showed that the percentage of progressive motility of the liquid semen after storage for 12 hours in andromed (A), andromed + sucrose 0.2% (P1) and andromed + sucrose 0.4% (P2) were 48.33%; 53.33% and 55% (P>0.05), respectively. In addition, the percentage of motility after 24 hours of storage in those extenders were 45%; 46.67% and 46.67% (P>0.05), respectively. The percentage of live sperm in A, P1 and P2 after 12 hours of storage were 70.33%; 73% and 73.33% while after 24 hours of storage, the percentage of live sperm in those extenders were 66.33%; 68.67% and 69.67%, respectively. In conclusion, the addition of 0.2% and 0.4% sucrose in andromed extender could maintain the quality of the spotted buffalo epididymal sperm after storage for 12 and 24 hours at 4 °C. Key words: epididymal sperm, spotted buffalo, sucrose, liquid semen, andromed
Penghambatan Proliferasi Limfosit Mencit Balb/c oleh Ekstrak Testis Sapi Bali: Peran TGF-β S N Depamede; A Rosyidi
Media Peternakan Vol. 32 No. 2 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

Testis extract (ET) of several animals such as rats, mice, boars, and bulls, has been reported able to suppress lymphocyte proliferation. In order to elucidate if ET of bali cattle has the suppressive activity, the present study was conducted. ET was isolated aseptically from the testis of bali cattle slaughtered at a slaughter house in Mataram, Lombok. After decapsulating, the testis were minced, homogenized (1 g tissue per 3 ml phosphate buffered saline, PBS, pH 7.2). The ET (40, 20, and 10µg/ml) was added on to lymphocytes isolated from Balb/c spleen, then cultured for 72 hours in RPMI media containing 10% foetal bovine serum in 96 well plates with the concentration of 250,000 cells per well, with- or without concanavalin A (10 µg/ml), or IL-2 (0.2 ng/ml). After 72 h of culture, cell proliferation was examined by colorimetric assay. The results showed that bali cattle ET were able to suppress the proliferation of lymphocytes in vitro through the process of apoptosis. Western blot analysis revealed that bali cattle ET contain TGF-β1. The suppression effects of the ET were eliminated by anti-TGF-β1 antibody. It can be concluded that ET of bali cattle expressed TGF-β  which inhibited lymphocyte proliferation through an apoptotic cell mechanism. The actual roles of TGF-β  in the bali cattle testis need to be elucidated further. Key words: bali cattle testis, lymphocyte, proliferation, TGF-β1, apoptosis
Response of Two Different Strains of Commercial Broilers to Different Dietary Amino Acid Allowance B Indarsih
Media Peternakan Vol. 32 No. 2 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The response of Cobb and Ingham strain commercial broilers to a range of dietary amino acids in a factorial experiment (2 strains x 2 sexes x 3 dietary treatments) with 3 replicates was evaluated. A total of 180 birds was sexed and weighed at one-day-old and randomly distributed to 36 brooders. Summit (246 g CP and 16.8 g lysine/kg) and dilution (120 g CP and 6.0 g lysine/kg) diets were prepared in isocaloric at 3100 kcal ME/kg. The estimated dietary level of crude protein for all birds at day-old was 240 g/kg and at 42 days were 120, 140 or 160 g/kg for females or 140, 160 or 180 g/kg for males. Body composition was measured at 42 days. Results revealed that strain Cobb males and females had a higher daily intake than Ingham, but Cobb females were less efficient in converting feed to weight gain than that of Ingham. The quantitative differences did not exist between genotypes, crude protein and lysine. Daily gain and feed utilization efficiency in both sexes were highest in group receiving the high protein diet. Lysine requirement was 14.58 g lysine /kg diet and 12.96 g/kg higher than NRC recommendation (1.05 % or 10.5 g/kg) for maximum breast meat and lowest abdominal fat. Key words: lysine, summit, dilution, breast meat, abdominal fat
Efektivitas Daun Murbei Sebagai Pengganti Konsentrat dalam Sistem Rumen in Vitro S Syahrir; K G Wiryawan; A Parakkasi; M Winugroho; O.N P Sari
Media Peternakan Vol. 32 No. 2 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

Mulberry leave has a great potential as animal feed because of its high nutrient content. It also has deoxynojirimycin (DNJ) compound, that is potential to increase fermentability of fibrous feed in ruminal system. An in vitro experiment was conducted to investigate the capability of mulberry leaves to substitute concentrate as feed for ruminant in increasing fermentability of fibrous feed in ruminal system. This experiment was carried out using randomized block design with five treatments and four replications. The treatments were: P0 (50% rice straw + 50% concentrate) as a control, P1 (50% rice straw + 37.5% concentrate + 12.5% mulberry leave), P2 (50% rice straw + 25% concentrate + 25% mulberry leave), P3 (50% rice straw + 12.5% concentrate + 37.5% mulberry leave), P4 (50% rice straw + 50% mulberry leave). Variables measured were fermentability (NH3 and VFA concentrations), pH, gas production, dry matter and organic matter digestibility. Data were analyzed using analysis of variance and duncan multiple range test was further used to test the significant differences. VFA concentration, dry matter digestibility and organic matter digestibility were significantly difference (P < 0.05) among treatments. However, there was no significant effect on the other variables. It is concluded that mulberry leave are able to substitute the concentrate and increased fermentability of fibrous feed in ruminal system. Key words: mulberry leave, rice straw, ruminal fermentation, in vitro
Kinetika Produksi Gas, Kecernaan Bahan Organik dan Produksi Gas Metana in Vitro pada Hay dan Jerami yang Disuplementasi Hijauan Mengandung Tanin A Jayanegara; A Sofyan; H.P S Makkar; K Becker
Media Peternakan Vol. 32 No. 2 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

This research was aimed to evaluate supplementation of tannin-containing forages, namely Rhus typhina and Salix alba into hay and straw diets on rumen fermentation parameters in vitro, especially on methane production. Supplementation was applied at 30% dry matter replacement from hay and straw diets. Treatments consisted of: H (hay), J (straw), RT (R. typhina), SA (S. alba), H:RT (hay:R. typhina, 70:30), H:SA (hay:S. alba, 70:30), J:RT (straw:R. typhina, 70:30), and J:SA (straw:S. alba, 70:30). Incubation was applied using Hohenheim gas production method for 24 hours. Variables measured after incubation were cumulative and kinetics of gas production, methane emission and organic matter digestibility. The results showed that supplementation of R. typhina and S. alba decreased (P < 0.05) percentage of methane production by 11.2% and 4.3% when added to hay, respectively; while it decreased (P < 0,05) percentage methane production by 15.8% and 6.1% when added to straw, respectively. Additionally, supplementation of these tannin-containing forages significantly increased (P < 0.05) organic matter digestibility of hay and straw. It was concluded that supplementation of tannin-containing forages could strategically be used to decrease methane emission from rumen fermentation in vitro, and at the same time increase the quality of basal diets. Key words: gas production, digestibility, tannin, methane, forage
Storage Capacity and Palatability of Wafer Complete Ration Based on Sugar Cane Top and Bagasse on Calves Y Retnani; W Widiarti; I Amiroh; L Herawati; K B Satoto
Media Peternakan Vol. 32 No. 2 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

Sugar cane top and bagasse represent agricultural by product which is potential as feed. This research was aimed to study of physical characteristics of wafer complete ration containing sugar cane top and bagasse during storage for six weeks. The treatments were alocated in completely randomized factorial design. The treatments as factor A were R0=80% concentrate + 20% native grass; R1=concentrate 80% + 20% bagasse; R2=concentrate 80% + sugar cane top 10% + bagasse 10%; R3=concentrate 80% + 20% sugar cane top. Factor B was storage period i.e., B1=0 week; B2=2 weeks; B3=4 weeks; B4=6 weeks. The result showed that the wafer complete ration contained natural grass, sugar cane top and bagasse did not affect the specific density and water activity, but the water content was the highest (P < 0.01) in ration containing natural grasses. Storage for six weeks affected (P < 0.01) water content, density and specific density, but did not affect water activity. Wafer complete ration palatability containing grass and sugar cane top was more preferred than sugar cane top combined with bagasse or bagasse without combination. Key words: wafer complete ration, sugar cane top, bagasse, storage, calves
Kualitas Rumput Unggul Tropika Hasil Ensilase dengan Bakteri Asam Laktat dari Ekstrak Rumput Terfermentasi B Santoso; B Tj Hariadi; H Manik; H Abubakar
Media Peternakan Vol. 32 No. 2 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The aim of this study was to evaluate the quality of napier grass (Pennisetum purpureum) and king grass (Pennisetum purpureophoides) ensiled with addition of lactic acid bacteria (LAB) prepared from fermented grass extract (LBFG). Four treatments were napier grass without additive (EG), napier grass with 3% (v/w) of LBFG (EGL), king grass without additive (KG) and king grass with 3% (v/w) of BLEF (KGL). Ensiling was conducted in silos of 225 g capacity at room temperatures (approximately 28 °C) for 30 days. Chemical composition of silage, fermentation products and nutrient digestibility were measured. LBFG added silages were higher (P < 0.01) in LAB population but lower in pH value (P < 0.05) compared to silages without LBFG. Dry matter (DM), organic matter (OM), crude protein (CP) and NDF contents of LBFG added silages tended to have higher value than silages without LBFG. Total and individual VFA concentrations in KGL silage were lower (P < 0.05) than KG silage. In vitro DM and OM digestibility and Fleigh point in both silages with LBFG tended to be higher than silages without LBFG. It was concluded that addition of 3% (v/w) of LBFG improved the fermentation quality of napier and king grass silages, as shown by the lower pH value and NH3-N concentration, and the higher LAB population number. Key words: tropical grasses, lactic acid bacteria, silage, fermented grass extract
Desain Pendekatan Komunikasi Partisipatif dalam Pemberdayaan Peternak Domba Rakyat . Hadiyanto
Media Peternakan Vol. 32 No. 2 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

This article explores the result of preliminary study conducted in two years (2006-2007) for designing participatory communication approach for small farmers’ empowerment. For that purpose basic data and information, such as; (a) level of knowledge, attitude and practices, (b) communication behavior and mass media accessibility, (c) focal problem and another potential resources have been collected. Data and information were gathered by survey method and participation action research. Meanwhile, participatory communication approach was designed by participatory communication strategy design (PCSD). Study indicated that good knowledge was not always followed by positive attitude toward daily management practices. From this point and based on validating focal problem, the existing of small famers group, and farmer’s communication behavior, participatory communication approach was developed. Small group communication forum supplemented with little printed media (leaflet) are still suited for increasing farmers’ knowledge, especially for unrecognized ones. The printed media should be the combination between pictures with simple texts (not more than four lines). Rationale (cause-effect model) for message treatment with informational, argumentation, and motivational message’s format is suggested. Key words: communication approach, empowerment, participatory, small farmer

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