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MEDIA PETERNAKAN - Journal of Animal Science and Technology

mediapeternakan
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Published by Institut Pertanian Bogor

ISSN : - EISSN : - DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Arjuna Subject : -

Articles 522 Documents

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Quality and Antioxidant Activity of Yogurt Supplemented with Roselle during Cold Storage El Latifa Sri Suharto; Irma Isnafia Arief; Epi Taufik
Media Peternakan Vol. 39 No. 2 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The purpose of this study was to determine the quality (physical, chemical, microbiological characteristics), total phenolic content, and antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) inhibition assay of probiotic yogurt supplemented with roselle flower extract (Hibiscus sabdariffa L) during cold storage. The experiment used treatment for types of yogurt as follows: cow’s milk probiotic yogurt + roselle, goat’s milk probiotic yogurt + roselle, cow’s milk yogurt, and goat’s milk yogurt. The yogurt was stored in cold storage and evaluated the quality and antioxidant activity variables on days 0, 3, 6, 9, 12, and 15th. The results showed that there were interaction (P<0.05) between types of yogurt and storage time on pH value and total lactic acid bacteria (LAB), but no interaction effect on viscosity. The types of yogurt significantly affected (P<0.05) aw, total titrable acid (TTA), total phenolic content, and antioxidant activity. Cow’s milk probiotic yogurt + roselle and goat’s milk probiotic yogurt + roselle were the best yogurt that contributed to a good quality and high antioxidant activity up to 15 d at cold storage.

Nutrient Digestibility and Performance of Male Pelung Chicken Fed Rice Bran-Based Ration Supplemented with Lime (Citrus aurantifolia) Juice Istna Mangisah; B Sukamto; H. I. Wahyuni; I. Estiningdriati; W. Saputro; L. Krismiyanto
Media Peternakan Vol. 39 No. 2 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Rearing Pelung chicken in Indonesia, in general, uses a ration with a high proportion of rice bran, even up to 60%. Considering the high contents of fiber, lignin, and phytic acid, feeding a high portion of rice bran will decrease nutrient digestibility. This study was aimed to evaluate the effect of lime juice supplementation of a rice bran-based ration on the utilization of nutrients in male Pelung chicken. Sixty four of 12 weeks old male Pelung chickens were used in the present experiment and assigned into a completely randomized block design. The treatments were supplementation of the ration with lime juice at the levels of 0, 1, 2, and 3 mL/100 g. Parameters measured were feed and nutrients consumption, protein, fat, and fiber digestibilities, apparent metabolizable energy (AME), rate of passage, feed conversion ratio (FCR), and daily body weight gain. The results showed that the supplementation of lime juice in the ration did not affect feed and nutrient consumption, but significantly (P<0.05) increased the digestibilities of protein, fat and fiber, AME, rate of passage, FCR and daily body weight gain. However, supplementation of 2 or 3 mL lime juice in the ration resulted in similar values of AME, and protein, fat and fiber digestibilities, and both levels of supplementation produced better protein and fat digestibilities, and AME when compared to the other treatments. It could be concluded that supplementation of 3 mL lime juice in the ration increased the digestion processes in the gastrointestinal and increased daily weight gain of male Pelung chicken.

Improvement on the Nutritive Quality of Napier Grass Silage through Inoculation of Lactobacillus plantarum and Formic Acid Saprilian Stya Hapsari; Suryahadi Suryahadi; Heri Ahmad Sukria
Media Peternakan Vol. 39 No. 2 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The potential availability of forage feed is high, but in reality this potential has not been able to meet the requirement of feed both in sustainable quantity and quality. Silage made with the use of liquid fermentation additive (FA) can be a solution for those problems. The use of different levels of FA and addition of Lactobacillus plantarum bacteria as well as formic acid were expected to improve the nutritive quality of napier grass silage. The first experiment was designed to measure the fermentative quality of napier grass silage. The treatments used were the levels of FA, L. plantarum, and formic acid supplementations. The experiment used a completely randomized design with a 3x2x2 factorial arrangement with 3 replications. The first factor was the level of liquid FA (5%, 7.5%, and 10%), and the second factor was the inoculation of L. plantarum (without and with inoculation of the L. plantarum), and the third factor was the addition of formic acid (without and with the addition of 0.15% formic acid). The second experiment was aimed to evaluate chemical and microbiological characteristics, and in vitro digestibility of selected napier grass silage. The results showed that napier grass silage from all treatments showed good qualities. There were interactions between FA, L. plantarum, and formic acid on DM content (P<0.05) and ammonia production (P<0.01). The use of FA showed an interaction (P<0.01) with the addition of L. plantarum and formic acid in Fleigh point. Ammonia production in rumen (P<0.01), total VFA (P<0.05), and in vitro digestibility (P<0.01) were significantly affected by the treatments. The optimal level of liquid FA was 7.5%. Based on the nutritive quality of silage, L. plantarum addition was as effective as control treatment to improve nutritive quality of napier grass silage through the increased of fermentation characteristics i.e., low pH, high DM product, high fermentation product (VFA), and digestible on rumen. Formic acid reduced ammonia production during ensiling and fermentation in rumen, but it was less effective in inhibiting the fermentation process when it was combined with L. plantarum.

Conception Rates of Holstein-Friesian Cows Inseminated Artificially with Reducing Frozen Semen Doses Anneke Anggraeni; T. Herawati; L. Praharani; D. Utami; A. Argis
Media Peternakan Vol. 39 No. 2 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Conception rates of Holstein Friesian (HF) acceptors were evaluated in artificial insemination (AI) mating using three frozen semen doses of the straws per volume of 0.25 cc. Frozen semen consisted of a standard dose by 25 x 106 sperms (L.1) and two reduced doses by 20 x 106 sperms (L.2) and 15x106 sperms (L.3). This field study was conducted in dairy cattle area of Lembang NBMCU, from August 2011 to March 2012. Frozen semens were obtained from two HF national progeny tested young bulls. Conception was detected by rectal palpation, around 60-90 days after AI mating. Conception rates (CR %) were calculated as a ratio between pregnant acceptors to the total AI mated cows. Some general linear model analyzes were conducted by considering fixed variables of semen dose (3), bull (2), inseminator (6) and parity (5). The results showed that three frozen semen doses did not significantly affect (P>0.05) on CR (%). By decreasing semen dose to L.3 gave CR (%) similar to L2 even to L1. It was concluded that frozen semen doses of HF bulls at the levels of 20 million to 15 million sperms per straw per volume of 0.25 cc resulted CR (%) similar to the standard one.

Prevalence and Characterization of Shiga Toxin-Producing Escherichia coli Isolated from Slaughtered Qurban Animal in Jakarta Province Siti Gusti Ningrum; R. D. Soejoedono; H. Latif; W. Arnafia; I. W. T. Wibawan
Media Peternakan Vol. 39 No. 2 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This study was conducted to investigate the presence of shiga toxin producing Escherichia coli (STEC) and the possibility of carrying rfbE gene and H7 flagellar on meat, liver, and stool samples collected from Jakarta Province of Indonesia. A total of 51 samples collected from meat, liver, and stool of slaughtered cattle from qurban festival were tested using conventional culture and multiplex PCR methods. STEC non O157 were detected in meat (5.3%) and stool (8.3%) with one isolate from stool carried H7 flagellar. However, all isolates were lacking of rfbE gene. In antimicrobial susceptibility tests, the STEC isolates showed antibiotic resistance to erythromycin and oxacillin. Overall, the result shows that meat and liver of this origin activity represents a potential risk to human health.

Partial Sequencing of 16S rRNA Gene of Selected Staphylococcus aureus Isolates and its Antibiotic Resistance Harsi Dewantari Kusumaningrum; Lita Handayani; Reni Nofrianti
Media Peternakan Vol. 39 No. 2 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The choice of primer used in 16S rRNA sequencing for identification of Staphylococcus species found in food is important. This study aimed to characterize Staphylococcus aureus isolates by partial sequencing based on 16S rRNA gene employing primers 16sF, 63F or 1387R. The isolates were isolated from milk, egg dishes and chicken dishes and selected based on the presence of sea gene that responsible for formation of enterotoxin-A. Antibiotic susceptibility of the isolates towards six antibiotics was also tested. The use of 16sF resulted generally in higher identity percentage and query coverage compared to the sequencing by 63F or 1387R. BLAST results of all isolates, sequenced by 16sF, showed 99% homology to complete genome of four S. aureus strains, with different characteristics on enterotoxin production and antibiotic resistance. Considering that all isolates were carrying sea gene, indicated by the occurence of 120 bp amplicon after PCR amplification using primer SEA1/SEA2, the isolates were most in agreeing to S. aureus subsp. aureus ST288. This study indicated that 4 out of 8 selected isolates were resistant towards streptomycin. The 16S rRNA gene sequencing using 16sF is useful for identification of S. aureus. However, additional analysis such as PCR employing specific gene target, should give a valuable supplementary information, when specific characteristic is expected.

Konsumsi daging sapi segar konsumen rumah tangga di pasar tradisional (kasus di Kelurahan Tugu Utara, kecamatan Koja, Jakarta Utara) D J Setyono; R Maharani; W Rindayanti
Media Peternakan Vol. 26 No. 1 (2003): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Konsumsi daging sapi segar konsumen rumah tangga di pasar tradisional (kasus di Kelurahan Tugu Utara, kecamatan Koja, Jakarta Utara)

Persentase berat karkas dan organ dalam ayam broiler yang diberi tepung daun talas (Colocasia Esculenta (L.) Schott) dalam ransumnya . Sumiati; W Hermana; A Aliyani
Media Peternakan Vol. 26 No. 1 (2003): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Persentase berat karkas dan organ dalam ayam broiler yang diberi tepung daun talas (Colocasia Esculenta (L.) Schott) dalam ransumnya

Persentase Bobot Saluran Pencernaan dan Organ Dalam Itik Lokal (Anas platyrhyncos) Jantan yang Diberi Berbagai Taraf Kayambang (Salivina molesta) dalam Ransumannya . Sumiati; A Sumirat
Media Peternakan Vol. 26 No. 1 (2003): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Persentase bobot saluran pencernaan dan organ dalam itik lokal (Anas platyrhyncos) jantan yang diberi berbagai taraf kayambang (Salivina molesta) dalam ransumannya

Identifikasi Gen Babi Melalui Runutan Klon cDNA Ovari . Jakaria; K Suzuki
Media Peternakan Vol. 26 No. 1 (2003): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Identifikasi Gen babi melalui runutan klon cDNA ovari

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