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INDONESIA
Indian Journal of Forensic Medicine & Toxicology
Published by Official Organ of Indian Association of Medico-Legal Expert
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Public Health
Arjuna Subject : Kedokteran - Epidemilogi
Articles 5,497 Documents
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Association among Vitamin D Deficiency with some Inflammatory Marker in Iraqi Patients with Autoimmune Thyroiditis Noor Alhuda Kh. Ibrahim1, Zaid Nsaif Abbas2, Wasnaa J. mohammad1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.125

Abstract

Background: Hashimoto thyroiditis (HT) is a common chronic autoimmune disease of the thyroid gland, characterized by painless goiter and elevated thyroid antibodies. Hypovitaminosis D (vitamin D insufficiency and deficiency) is common among patients with HT.Method: : the study include (60) subjects compared with 30 apparently healthy control group were visiting nursing home hospital in Baghdad medical city, Levels of serum thyroid-stimulating hormone (TSH), free-triiodothyronine (FT3), free thyroxine (FT4), TgAbs, and TPOAbs were determined with automated immuno chemiluminescent assay (ICMA) kits (Abbott Laboratories, IL, USA). Levels of serum 25-hydroxyvitamin D 25(OH) D) were determined using a competitive protein-binding assay (Roche Diagnostics, Mannheim, Germany). The inter-assay variation coefficient for 25(OH)D measurement was 8.5%.Results: the level of the study parameters in autoimmune thyroiditis which show significant correlation in age ,BMI, vitamin D, anti TPO antibody,IL-17, TNF-? and thyroid function (p<0.05) (p<0.01) test but calcium shoe non significant correlation between patients and control group.Conclusion: The present work shows a significant association between circulating 25(OH)D and HT, also IL-17.
A Statistical Study on Microorganisms that Cause Tooth Decay and Prevention and Treatment Methods Widad sameer jaaz1, Ohood Salman Jawad2, Hassanain Jwad Abid Al Hussein1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.126

Abstract

The current research includes knowledge of the effect of microbes on dental caries, by providing the environment suitable for the growth of bacteria and fungi that cause this decomposition, fermentation and decay and thus erode the texture of the tooth and then full tooth decay. These are the macrophages that lead to tooth decay (streptococcus species, Staphylococcus aureus, Candida, Lactobacillus, Actinomycosis, Micrococcus, Antibacterials, Bacillus, Actinobacillus) in different percentages: 33.3%, 21.3%, 14.7%, 12.1%, 8.2 , 4.5%, 3.4%, 1.3%, 1.2%), respectively. There are many other factors that help tooth decay occur: the host (tooth and saliva), microorganisms in the form of tooth plaque, substrate (diet).
In vitro Antifungal efficacy of Saccharomyces Cerevisiae Mediated Nanoparticle Against Resistant Candida Albicans Isolates Hussein A. R. Abbood1, Bari L.Mohammed2, Hawazin A.Aboid2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.127

Abstract

Dry yeast Saccharomyces. cerevisiae was obtained from markets in Kirkuk and used to study their ability to prepare silver nanoparticles and shows that the silver nitrate solution at 1Mm changes its color to brown and is an initial indication of the formation of nanoparticle particles and X-ray diffraction peaks were measured at (111), (202) and (220) respectively. Particles also appeared in clear minutes in the scanning electron microscopy images at a size of 44 nanometers . A total of 103 vaginal swabs were taken from women who suffered from vaginitis from the Kirkuk General Hospital. The results revealed that Candida types were responsible for 67% of the cases. Candida albicans, C.glabrata, C.tropicalis, C.krusei and C.parapsilosis were isolated. 30.8%, 22.8%, 20%, 15.8% and 11.5%, respectively . In addition, the sensitivity of the drug in the drilling method against the isolates of Candida albicans showed high resistance to fluconazole and gresiofulvin antagonists, with 100% of gresiofulvin (85%) of fluconazole. For nystatin, Resistance ratios (14%) The solution of the nano-silver particles recorded from Saccharomyces Cerevisiae yeast was also shown. had a significant inhibitory effect on Candida albicans, a multi-resistance yeast antimicrobial resistance (21,21,22mm) at 100% and (10,13,10mm)at 50% and inhibition(5, 4,5 mm) has a 35% dilution and a 25% concentration did not receive any inhibition.
Application of Chemical Techniques in the Manufacture of Some Industrial Dental Materials Hamed Ibrahim Mohammed1, Aara Ali Hadi1, Hassanain jwad abid al Hussein1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.128

Abstract

This method of manufacturing Separating Medium was used in the field of chemical polymerization, in which a semi-gelatinous liquid was placed on the dental kits to prevent the adhesion of the gypsum material to the dermis made of hot processed polymer. After the experiments on the plant seeds, specifically the flax plant, the final extraction of the substance has been reached. It has given all the desired good specifications that are used in the field of dentistry. It is known that this material is a polar compound was diluted with distilled water after preparation. It is worth mentioning that 10% of the industrial pigments have been added to give them the red or pink color as these colors are desirable and common in the commercial markets as well as It is recommended for the dental products manufacturers, as well as 10% of the original alcohol and 1% of formalin to prevent the growth of bacteria and other types of microorganisms that cause rotting and decomposition of the manufactured material.
Isolation and Identification of Pantoea agglomerans from Open Heart Operations Unit of Marjan Hospital in Hilla City Enas R . Al-Kidsawey1, Kawther H. Mhdi1, Mohammed A. Al kaif1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.129

Abstract

The nosocomial infection of Pantoea agglomerans has become an important cause of many systemic infections in humans, and considered of present study was to isolate and identify this pathogen from open heart operation unit of Marjan hospitals in Hilla city during a period from April to September 2018, (19) isolates of Pantoea agglomerans. Was isolated from (150) swaps which were collected from the environmental of open heart operation unit. 8/30 isolates from system, 3/35 from bed, 3/25 from earth , 3/35 from door and 2/25 from nurse hands isolated identification of microbes were done according on the morphological, cultural ,microscopically characterization and list of biochemical test. To emphasize the identification of Pantoea agglomerans. We used the API20 technique biochemical strips ( biomerieux SA/marc y-1 Etoile France).The antimicrobial susceptibility pattern of the isolates was studied using different types of antibiotics. The microbes showed resistance to the routinely used antibiotics.
Immunological and Molecular Study of Human Cytomegalovirus contribution to Anemia in patients with Chronic Kidney Disease Zahraa Abdulridha Baqer AL-Fayyadh1, Musa Nima Mezher1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.130

Abstract

Background: Renal failure is a condition in which the kidneys fail to remove metabolic end-products from the blood and regulate fluid, electrolytes and pH balance of extracellular fluids. The aim of current study was to investigate the role of human cytomegalovirus in renal failure and its contribution to anemia among patients with renal failure. Methods: This study was carried out at AL-Sadder Medical City Hospital during the period from December 2018 to February 2019. The study involved a total of 50 patients (32 males and 18 females) with kidney disease with age range between (20-70) years and 20 healthy individuals considered as controls. The human cytomegalovirus antibody were detected by cassette (rapid test) and compared the results of cytomegalovirus diagnosis (by rapid test) with molecular technique (Real time-Polymerase chain reaction ) results. Erythropoietin hormone concentration was detected by ELISA technique. Results: Data obtained from rapid test showed that positive patients for human cytomegalovirus were 32(64%) for all cases, while patients negative for human cytomegalovirus were 18(36%) compared with those of controls. Real time-Polymerase chain reaction amplification for presence of human cytomegalovirus DNA in serum samples revealed that human cytomegalovirus genome was detected in 10(20%) of the 50 serum samples. Erythropoietin hormone showed lower concentration in patients than controls. Erythropoietin showed significant decrease (P<0.05) in all groups of patients compared to those of control group. Conclusion: Human cytomegalovirus seemed to have relationship with chronic and acute renal failure and can affect patient’s immune status. Also, the decrease of erythropoietin hormone is the mean cause of anemia in renal failure patients.
Comparison between HSP70 Levels in Acute and Chronic Coronary Artery Diseases Wurood A.Kadhim1, Noora M.Kareem2, Samar A. Al-Shami3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.131

Abstract

Background: Heat shock proteins are a family of endogenous proteins that act as molecular chaperon and increase in different stress situations like heart disease and atherosclerosis. Therefore, the aim of this study was to investigate whether there is a difference between Hsp70 level in the blood of myocardial infarction patients and apparently normal population; and between recent myocardial infarction patients and previous myocardial patients using immunocytochemistry technique. Method: Peripheral blood sample was taken from 50 patients with history of myocardial infarction divided into two groups (11 with acute or recent myocardial infarction and 39 with old or chronic myocardial infarction. Another 50 apparently healthy individuals were taken as a control group. Heat shock protein 70 level was measured by immunocytochemistry technique. Results: There was significant rise of heat shock protein 70 in myocardial infarction patients as compared with control group. Also, a significant decrease in heat shock protein 70 level in the chronic myocardial infarction patients group as compared with the acute myocardial infarction patients group. Conclusion: this study showed that heat shock protein 70 increases in acute myocardial infarction patients’ but its level decreases in chronic myocardial infarction. So heat shock protein 70 can be used as a biomarker to differentiate acute, from chronic, myocardial infarction and may be helpful as an indicator of acute myocardial infarction.
A Statistical Study to Determine the Factors of Vitamin D Deficiency in Men: the City of Baghdad as a Model Suad Turky Ali1, Hind Tahir Qadir2, Shaimaa Khalid Moufak1, Mohammed Ali Mohammed Al-Badri3, Mohamme
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.132

Abstract

Background: Exposure to sun in the sunny days for 10 to 15 minutes a day and two to three times a week is enough to get vitamin D requirements in the majority, but people with darker skin need to be exposed to sun for longer periods to get their vitamin requirements. The purpose of this research was to achieve a statistical study to know and determine the factors behind deficiency of vitamin D among men in Baghdad city in addition to knowing the most important diseases associated with its deficiency in order to inform and educate the community as well as to raise awareness of the society about the role of this vital vitamin, prevention of its deficiency and to propose appropriate treatment for it. Methods: The research was conducted in several private clinics in Baghdad throughout 2017. The investigation was including study of 500 cases of men who were randomly selected. The cases were divided according to age groups into two groups: the first group was (20-40) years, while the other group was (more than 40 years). Also, the cases were classified according to vitamin D level to sufficient, insufficient and deficient. Results: The results obtained from the study showed that the percentage of vitamin D insufficient and deficient were 69.6% and 18.8%, respectively, while the sufficiency percentage was the lowest and scored 11.6%. Common chronic diseases, such as high blood pressure and diabetes as well as sleep disorders and muscle fatigue, were also followed in the cases. The results illustrated that 47.6% of men cases had diabetes and 39% had high blood pressure, while 28.2% and 17.2% of them had muscular fatigue and sleep disorders, respectively. These chronic diseases and symptoms were related in one way or another with Vitamin D deficiency. Conclusion: Vitamin D can be given for preventive and therapeutic purposes.
Mutation Evaluation in P53 exon 5 in Iraqi AML Patients with 4 Growth Levels Ishtar Imad1, Abdulameer N. Ghaloub1, Mehemt Ozaslan2, Alaa F. Alwan3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.133

Abstract

Background: The role of TP53 is not limited to repairing damaged DNA and regulating its proliferation, it also activates other repairing genes and prevents mutated DNA from multiplying which prevents malignancy formation that’s why it’s called “ DNA`s gatekeeper”. The aim of current study was to evaluate the role of Exon 5 of P53 gene in the development of AML in Iraqi patients. Method: Sixty newly diagnosed AML patients at Baghdad (haematology national centre) were involved in ccurrent study. Peripheral blood samples were collected in EDTA tubes then they followed a month after receiving 3 and 7 AML treatment regimens to compare mutational status pre and post treatment. The patient divided into 4 age groups based on growth level (0-15 years, 16-40 years, 41-65 years and 66 years and above) in a 15 sample for each. Results: We uncovered transcriptional downregulation of significant p53 acetyltransferases in both CN-AML and APL, joined by expanded Mdmx protein articulation and deficient Chk2 protein enactment. Mutation study on exon 5 of P53 gene showed no differences in gene sequence from the standard sequence of NCBI geneBank sequence. Conclusion: Exon 5 of P53 gene not included in the AML causes since the patients of this study showed no alteration in sequence from the reference sequence
Assessment of Antagonistic Effect of Alcoholic Extract from Cyanophyta (Spirulina Platensis) Against Several Human and Plant Derived Pathogenic Fungi Raghad J. Fayyad1, Rasha Saad Nuaman2, Noor T. Hamdan3, Rasha Saatam Hameed4, Sara A. J. Maliki5
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 1 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i1.134

Abstract

Background: Blue-green algae have been proved to producing different bioactive compounds. For this reason Spirulina platensis isolates have been used in the present study as a biocontrol agent against several human and plant pathogenic fungi. Current study was aimed to investigate the use of crude extract of Spirulina platensis as antifungal agent by determining the antagonistic activity of methanolic extract of Spirulina platensis against several human and plant pathogenic fungi. Also, to determine the antifungal phytochemicals within algal alcoholic extract through GC-mass analysis. Method: Four different concentrations of alcoholic extract were prepared (100,75, 50 and 25mg/ml) from algal isolates and their antagonistic activity was investigated against molds isolated from spoiled fruits (two isolates of Aspergillus niger, Aspergillus flavus, Mucor sp. and Botrytis sp.) and against several Candiada spp. (Candida albicans, Candida glurbrate, Candida fameta, Candida lustrans) which were isolated from different clinical sources. Results: The results showed that algal extract displayed wide range of antagonistic activity against tested fungi depending on applied algal extract concentration and tested fungal species. Also, GC-Mass data analysis had been performed for algal extract and 36 different bioactive chemicals have been identified including eight compounds authenticated as having antifungal activity. Conclusion: Spirulina could be used as alternative drug to treat Candida sp. infections as well as added to food industries to enhance their nutritional value and simultaneously decreasing the possibility of food spoilage by molds.

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