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INDONESIA
Indian Journal of Forensic Medicine & Toxicology
Published by Official Organ of Indian Association of Medico-Legal Expert
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Public Health
Arjuna Subject : Kedokteran - Epidemilogi
Articles 5,497 Documents
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Glycodeline-A , PlGF Together with ?hCG as Prognostic Markers in First Trimester Pregnancy Loss for Iraqi Women Mohammed H Dawood1 , Halla G Mahmood2, Afraa M Nedawi3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11864

Abstract

Objective: estimate the scale of Glycodeline-A,PlGF, together with ?hCG in first trimester pregnancy lossin Iraqi women .Methods: A subject:patients and control study, begining in April 2019 and finish the work at March 2020,study include 85 women,45 of them as healthy control which haven,t any complication at the first trimesterstsrting from the first day of LMP , 40 of them as patients which have abortion at the same time,testsincluded evaluate the scale of ?hCG,Glycodeline-A,PlGF By ELISA method.Results: scale of ?hCG are significantly higher in healthy controls than in patients , while scale ofGlycodeline-A , PlGF was significantly lower in healthy control than in patients,and there is significantcorrelation among each of ges.age with ?hCG and PlGF P <0.05.Conclusion: This study suggest that the significant role of Glycodeline-A,PlGF in etiologies of first trimesterpregnancy failed and some of them was related to ?hCG in this time and the age of patients wasn,t effect onall of these parameters.
Analysis of Single Nucleotide Polymorphism in Fim H Gene from Uropathogenic E.coli Sabreen Fleih Neno Al-Fatlawi1, Laith Abdul Hassan M. Jwaid2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11865

Abstract

Background: Urinary tract infection is defined as presence of microbial pathogens in the urinary tract withassociated symptoms. The infection affects both lower and upper urinary tracts and is known as acute cystitisand polynephritis respectively. This study done to detect the variation of single nucleotide in FimH gene andits correlation with the pathogenicity of uropathogenic Escherichia coli caused the urinary tract infection.Materials and Methods: The study group included 150 patients with Urinary tract infection. There were120 (80%) female and 30 female (20%) their age ranges between (5-55) years, seen in Al-Hussein TeachingHospital for the period from January 2019 to March 2019. The dominant form (48.39%) of patients withurinary tract infection caused by UPEC was found in the age group of 24-39 years old. Urine sampleswere collected from the patients. Bacterial isolation was identified by microscopic examination and culturecharacteristic on selective media MacConky agar, Eosin Methylene Blue and Chrome agar. Then the isolatedwere diagnosis by Vitek®2 system.Results: Bacteriological results showed that 62(41.3%) isolates were characterized as Escherichia coli.Genomic DNA was extracted from the bacterial culture for further molecular study to reveal C640T andT591A fimH gene Polymorphism. Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) technique was used for this purpose and digestion of the amplified DNA products byScarFI1390I and BsrI endonuclease respectively gave fragments with different molecular sizes whichexpress certain genotypes. This study detected that the prevalence rate of single nucleotide polymorphismC640T FimH was (85.48%), endonuclease enzyme only affects the C allele sequence and it does not haveany influence on the T allele sequence. The prevalence rate of single nucleotide polymorphism T591AfimH gene was (80.64%), endonuclease enzyme only affects the T allele sequence and it does not have anyinfluence on the A allele sequence.The result show only 62 isolates (41.3%) were given typical morphological characteristics and biochemicaltests related to E. coli, while the rest 88 isolates (58.6%) belonged to pathogenic bacteria from differentgenera.
The Impact Of Child Abuse and Neglect on Oral Habit among 10-11 Years Old Primary School Students in Baghdad /Iraq Wasan Muwafaq Almirhij1 , Ban Sahib Diab2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11866

Abstract

The purpose of this study was to assess the impact of child abuse and neglect on oral habits among 10-11 years old primary school students in Baghdad/Iraq. This observational study included 600 studentsdistributed to 373 male and 227 female at different economic and social levels with age 10-11 years old offourth and fifth class of the elementary schools in the Karkh city. Information on child abuse and neglect andpresence or absence of oral habits was obtained from a structured, self-administered questionnaire from thestudents who were willing to participate in the study and their parents. The data was collected, summarizedand statistically analyzed. The child abuse and neglect had effect on presence of oral habits in children.
Genetic Study of the Effect of the Toxoplasma gondii on Somatic Cells of the Bone Marrow in Female Mice and a treatment Attempt by Laurus Nobilis Leaf Extract Ghfran Shihab Ahmed Al-badri1, Aysir Salih Mohammed2 , Omar Raheem Khalaf2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11867

Abstract

This study aimed to find out the ability of T. gondii parasite to induce genetic changes in the bone marrowcells of female mice and to try therapeutically for it using bay leaf extract. The parasite was extracted from(23) placentas for aborted women and injected into the 42 mice with an average weight of (20-30) gram. Itwas divided into six groups at a rate of (3 females) per group. The first group doses the oil only as a negativecontrol, the second group was infected with the parasite as a positive control, while the four study groupsdose the plant extract of the bay leaves for (14) days and at doses of (15, 10, 5, 1) mg. for Kg after a periodof infestation with the parasite for 14 days. The results of the chromosomal changes showed the type ofchromatid fracture among the females injected with the parasite + the extract and with all its concentrationssignificantly reduce the level at (P <0.05). The concentration was 15 mg/kg, the lowest as the results reached(9.33 ± 1.33) compared to the control of the parasite (44.00 ± 2.30), while the centromere fracture showedAll concentrations showed a significant decrease at the level of (p<0.05) and showed concentrations 15and 10 more decreases with a result of 2.66 ± 1.33 for both concentrations compared to the control of theparasite, As for the annular chromosome, all concentrations showed a significant decrease at the level of(p<0.05) for females injected with the parasite extract, and the concentration was 15 mg/kg, the lowest was(6.66 ± 1.33) compared to the control of the parasite.
Genetic evaluation of rs36513 SNP of UIMC1 and Biochemical Parameters in Early Menopause in Babylon City, Iraq Rana A. M. Shaker1 , Maha F. Mohammed2 , Bushra j. Al-Rubaey3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11868

Abstract

Premature menopause or Early menopause (POF) is a condition charecterised by amenorrhea,hypoesteronigism, and elevated serum gonadotrophin level in women younger than 40 years old. This workaimed to evaluate the association of candidate genetic polymorphism in called trans membrane protein 150B (TMEM150B ) and ubiquitin interaction motif containing 1 (UIMC1) also the hormonal changes withPOF in one district of Iraq (province of Babylon) by enrolling Arab ancestors. The genetic variants thatwould be studied in this project were carefully selected according to the recent findings in this field, and byemployed several specialized databases . The selected variant are transmembrane protein 150B (TMEM150B) and ubiquitin interaction motif containing 1 (UIMC1) and their relation with premature ovarian failure.This study include (200) females , their ages between (35-40 years) and body mass index in normal andoverweight, (50) of them were with amenorrhea for 6 months, and the other (150) apparently healthy ascontrol group. Concerning rs365132, the genetic association with POF showed that there was no significantallelic or genotypic association recorded (P > 0.2 ) , except that the patients group was significantly deviatedfrom Hardy-Weinberg equilibrium exact test (P=0.023). For rs11668344, the genetic association with POFshowed that there was no significant allelic or genotypic association recorded (P > 0.2 ) , except that thepatients group was significantly deviated from Hardy-Weinberg equilibrium exact test (P=0.023). Thehormonal results of the present study showed significant increase in FSH in patients compared with controlsgroup. (P < 0.0001) and this study showed significant decrease (p? 0.001) in EST in patients compared withcontrols group. As a conclusion , the study conclude that rs365132 and rs11668344 genes variants do notrepresent genetic factors which can modulate the development of POF in Arab population from Babylonprovince .
Prevalence of Campylobacter Species in Diarrheal Samples of Children Less than 10 Years Ghada N. Khalaf 1 , Thaanaa R. Abdulrahman²
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11869

Abstract

Introduction: Campylobacter spp. is one of the most common causes of diarrheal diseases all over theworld, with a rapid acquisition of antibiotic resistance.Objective: Detect the frequency of Campylobacter species in diarrheal stool of children under ten years byconventional and molecular methods, and detect the bacterial ability to produce biofilm.Methods: A total of 200 children presented with diarrhea had been enrolled in this study. Campylobacterwas isolated and diagnosed primarily by characteristic features on Gram stain, culture media, biochemicaltests, and tested against 13 antibiotics by disc diffusion method. The ability of biofilm production wastested by crystal violet quantitative ELISA microtiter plate assays. Then Campylobacter spp was detectedby Multiplex PCR using species specific genes.Result: The prevalence of Campylobacter spp. was 17% by conventional methods and 15% by molecularmethod. The results of antibiotic susceptibility test showed that, there is complete resistance (100%)to cephalothin, ampicillin, and clindamycin for both species, full resistance (100%) to trimethoprimsulfamethoxazol and erythromycin by C.coli, and high resistance (92.3%) to trimethoprim-sulfamethoxazoland erythromycin by C.jejuni, while the lowest resistance was to nalidixic acid and amikacin (7.7%) byC.jejuni, and (12.5%) to tetracyclin, amoxicillin, amikacin, chloramphenicol, and ciprofloxacin by C.coli.The frequency of biofilm production in all positive Skirrow’s culture was (35.29%) as 12 out of 34 positiveisolates, ranging from mild to severe biofilm formation. By PCR assay, 64.7 % (22 of 34) positive Skirrow’sculture were also positive based on hipO gene specific for C.jejuni, while the prevalence of asp gene was(23.5%).Conclusion: The prevalence of Campylobacter spp. was 17% by conventional methods and 15% by molecularmethods, most of Campylobacter jejuni and Campylobacter coli isolates were MDR, and sensitive only tolimited number of antibiotics, many Campylobacter isolates produce biofilm, there was highly significantcorrelation between hippurate hydrolysis results and molecular detection of Campylobacter spp. dependingon hipO and asp genes.
Clinical Evaluation of Recurrent Aphthous Stomatitis and Its Correlation with Helicobacter Pylori Shaimaa A. Saeed1 , Taghreed F.Zaidan2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11870

Abstract

Recurrent aphthous stomatitis is a disorder distinguished by ulcers restricted to the oral mucosa. Because ofthe histological similarities between peptic ulcers and recurrent aphthous stomatitis and the identified roleof Helicobacter pylori in peptic ulcer, the possibility of bacterial involvement in the progression of aphthaehas been suggested. Helicobacter pylori are one of the most common, well-known pathogenic agents in thedevelopment of peptic ulcers. Some investigators have shown a relationship between Helicobacter pyloriand recurrent aphthous stomatitis. However, this relationship is controversial. The aims and objectives ofthis study were 1- Clinical evaluation of patients with recurrent aphthous stomatitis.2- Determination of theassociation between Helicobacter pylori and recurrent aphthous stomatitis using the urea breath test.Eighty-five subjects were participated in this study; they are divided into two groups. Fifty patients withrecurrent aphthous stomatitis, which at least occur four, or more times per year, thirty-five healthy subjectsof the same age group as controls.The results showed that ,the mean age of patients with recurrent aphthous stomatitis lie around 3rd decadeand the percentage of female with recurrent aphthous stomatitis was higher than male,the clinical findingsshowed that the number of patients with minor recurrent aphthous stomatitis was higher than patients withmajor and Herptiform recurrent aphthous stomatitis , No difference between the number of patients who hadsingle recurrent aphthous stomatitis and number of patients who had multiple recurrent aphthous stomatitiswas found . According to the site of ulcers, the result founded that the number of patients who had RAS onthe non-keratinized mucosa was higher than patients who had RAS on the keratinized mucosa and those whohad recurrent aphthous stomatitis on keratinized and non-keratinized oral mucosa.Regarding the laboratory findings, the percentage of patients with recurrent aphthous stomatitis who ureabreath test positive was higher than percentage of control group,also the mean value (Delta Over Base) ofurea breath test in recurrent aphthous stomatitis patients was statistically higher than that of healthy controls.By these results we concluded that there was a correlation between recurrent aphthous somatitis and H.pyloriby using urea breath test in patients with recurrent aphthous stomatitis when compared with controls.
The Effect of An Educational Curriculum According to (K.W.L) Strategy on Some Mental Abilities and Learning Some Basic Football Skills for Students Ali Mahdi Hassan1 , Yousef Abdul Karim Yousef Abu Ghneim2, Hayder Naji Habash Alshawi 3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11871

Abstract

The research problem was centered in the fact that the student usually cares about playing without using themental abilities in the skill and what is known about the skill and what he wants to know and what he learnedfrom it, and through the experience of the researchers, they found that it is necessary to review the strategiesand methods of teaching used and the introduction of modern educational methods based on the needs ofstudents and their inclinations And their psychological desires and needs, and one of the most importantmodern strategies is the (KWL) strategy that works to stimulate students ’previous knowledge and make ita starting point for linking it with new information to renew the meaning of learning and increase studentconfidence and his ability to plan, monitor and strengthen He did his work, as well as developing mentalabilities that are one of the main goals in education, as traditional methods that focus on preserving andretrieving study material only do not necessarily lead to developing mental abilities, and that the researchproblem manifests itself in identifying the impact of using the KWL strategy in Some mental abilities,cognitive achievement, and learning some basic skills of football for students. The research objectives wereto prepare a teaching strategy (KWL) for students and to identify their effect on mental capabilities and insome basic skills for football for students. The researchers assumed that there were no significant differencesin Mental abilities and learn some basic football skills for students.
Molecular and Phenotypic Detection of Some of the Coded Genes for Virulence Factors in Mrsa and Mssa Isolated From Different Clinical Cases Noora Hamid Ibrahim1 , Ahmed Mohammed Turki2 , Jamal Abd Al-Rahman3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11872

Abstract

The current study included the collection of 100 clinical samples obtained of patients reviewed inthe laboratories of Ramadi hospital for women and children and Hit General Hospital and some civillaboratories within the city of Ramadi, including Bacterimea samples, Urine, abscesses, heart inflammation,wounds, rhinitis, osteoporosis, otitis ,Laryngitis, sputum (35, 20, 5, 6, 7, 5, 8, 7, 4, 3) samples respectivelyto investigate the presence of staphylococcus aureas . The results of diagnosis of bacterial isolates showedthat there were 55 bacterial isolates belonging to the S.aureus bacteria after they were grown on the culturemedia and examined microscopic as well as the use of some of the tests on the chemical, and the diagnosiswas confirmed using the Vitek2-compact system .The diagnostic results were confirmed by the use ofgenetic diagnosis. The results of the phenotypic investigation were identical to the results of the geneticinvestigation using the polymerase chain reaction technique in terms of possessing isolates producing bothhemolycin , on the HlA encoding genes for the production of type and hlB encoded for typeproduction, For plasma blood on the co-a gene, and the resistance isolates of methylation on the mecA gene.
Histological Changes Induced by the Fusarium Graminarum Silver Nanoparticles in Some Organs of Male Albino Mice Sahar A. H. AL-Sharqi
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11873

Abstract

The aim of this study was to investigate the effects on male albino mice of the histological change ofFusarium graminarum, silver nanoparticles (AgNPs), and the traces of these nanoparticles in the liver,kidney, and small intestinal. The experiment consisted of 40 mice divided into two groups, the first group of20 mice was considered to be the control animals and the other group was 21 days of treatment with a dose ofAgNPs (0.1ml / day). Microscopic examination of the liver showed disturbed structure of the hepatic lobule,hepatocyte hypertrophy with severs infiltration of inflammatory cells, kupffer cell proliferation, coagulates,necrosis and hydropic degeneration. In addition, the kidney section showed lobulated glomeruli, a large areaof hemorrhage, congested blood vessels that showed thickening in their walls, changes in degeneration andinflammatory cell infiltration.

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