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INDONESIA
Indian Journal of Forensic Medicine & Toxicology
Published by Official Organ of Indian Association of Medico-Legal Expert
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Public Health
Arjuna Subject : Kedokteran - Epidemilogi
Articles 5,497 Documents
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Bacteriological Study and Investigation of Some Virulence Factors of Proteus mirabilis Bacteria Isolated from Urinary Tract Infection Patients in Ramadi City Nada Ali Attallah1 , Muthana Badeea Farhan2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11840

Abstract

Twenty isolates of Proteus mirabilis were diagnosed with 8.33% of the total of 240 samples that were isolatedfrom urinary tract infections of patients returning to Ramadi General Teaching Hospital and Women’s andChildren’s Hospital, after conducting biochemical tests and confirming them with VITEC 2, the results ofthe qualitative investigation of the UreR, ZapA, and MrpA genes showed that all isolates (20) of P. mirabilishad these three genes with a percentage of 100%. The results of the nitrogenous bases sequences confirmedthe agreement of the diagnostic results of P. mirabilis isolates with the isolates recorded in NCBI usingthe( MEGA7) program with 100% match for each of the MrpA gene isolation number( 17) and ZapA geneisolation number 12, as for the ZapA gene, isolation number (2), seven mutations of the type of equivalenttransition were shown, and one mutation, a type of non-equivalent substitution, transversion, with a matchratio of 98%, while for the UreR gene for isolates No.( 5 )and No.( 7), it showed four mutations equivalentto the same sites and for the same Sequence ID. With a similar ratio of 99%, it can be inferred in principlethat the ZapA, UreA, and MrpA genes were found in the same genus but in other species and strains with thesame sequence of nitrogenous bases.
Molecular and Parasitological Study on Selected Opportunistic Intestinal Parasites in Immunocompromised Patients Mahmoud Shehab Al-heety1 , Sarab Fawzi Al-Ani2, Maher Ali Jasem3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11841

Abstract

The common opportunistic parasites which cause morbidity and/or death in immunocompromisedpopulations are predominantly gastrointestinal ones. This study explained the molecular and parasitologicalaspects on selected opportunistic intestinal parasites in selected groups of immune compromised patientsfrom Al-Anbar hospitals and private laboratories.In the current study, were taken (130) stool samples from immune compromised patients divided into threegroups (50) chemotherapy recipient, (40) chronic renal failures, (40) diabetic Mellitus, and (30) samplesapparently healthy as control. A questionnaire was filled out on each subject including all personal andmedical history. Stool samples were collected from all groups for detection of the parasites.The results were displayed the ratio of opportunistic parasites in (55.3)% of patients, and healthy controlwas (3.3)%. The results showed that the highest group had parasites in those with chemotherapy (64)%,chronic renal failure (57.5)%, and diabetic (42.5)% individually. The highest rate of infection appeared withBlastocystis hominins(26.1%), Cryptosporidium(22.3%), and Cyclospora cayetanensis (8.4%). The age ofthe patient was ranged between 4-66 years, the Mean was 47.5 and deviation was± 9.12, also, the weightof the patient was ranging between 14-85, the mean was 70.3 and deviation ± 8.07. Three cases of mixedinfection were detected.
Immunocytochemistry of In vitro produced mucin from HT29- MTX cell line Immunocytochemistry of In vitro produced mucin from HT29- MTX cell line
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11842

Abstract

Mucus represents the first line of defense against pathogens and damage in the human intestine. the majorcomponents of mucus are mucins which are high molecular weight glycoproteins providing potential bindingsites for both pathogenic and commensal microorganisms. To study host-enteric pathogen interactions,human intestinal cell models are widely used. Epithelial cell lines derived from human colon carcinomasproviding useful experimental alternatives as getting normal intestinal cells is not easily applicable.In the current study, we described a condition enhances HT29-MTX cells to produce continuous mucuslayer. Immunocytochemistry was used to visualize Muc 2, the most secreted mucin from HT29-MTX cellsusing anti Muc2 antibody.The results showed that incubation of HT-29 MTX cells with shaking and providing cells with fresh mediumonce to twice daily for three weeks can be the best way to mimic the natural behavior of the intestine whichenhanced the seeded cells to produce the mucin in vitro.
Deleted Maysoon Abdul-zahra Merdaw1 , Zeina Anwar Jaffar2 , Zahraa Mohammed Ali3 , Heba Hashim Khalil4
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11843

Abstract

a
Spectrophotometric Determination of Ampicillin with Sulfanilic Acid by Oxidative Coupling Reaction Samar Ahmed Darweesh1 , Hamsa Muneam Yaseen2 , Ruaa Muayad Mahmood3 , Rokayia Samir Al-Khalisy4 , Hu
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11844

Abstract

Sensitive, economic, simple and accurate spectrophotometric method for estimation of ampicillin in bulkand dosage form is described. Ampicillin was oxidized by sodium hypochlorite and coupling with sulfanilicacid in the presence of sodium hydroxide to obtain a stable yellow colored chromogen which exhibit amaximum absorption (?max) at 400 nm. The optimum conditions were carefully evaluated .Plot of absorbance against concentration was linear over the range (50-300 µg.ml-1). This method was applied tothe estimation of ampicillin in pure drug and commercial formulations successfully.
Study of Smoking Effect on Some Liver Functions, Blood Pressure and Some Haematological Parameters in Smokers in Al-Muthanna Province Duaa Hamad Hamza1 , Wafaa Abdulmutalib Naji2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11845

Abstract

The aims of this study was to investigate the changes that occurred on some liver functions and somehaematologicl parameters and blood pressure of smokers. The current study is carried out in in Al- Muthannaprovince.The study included 200 men, (100) smokers and (100) non smokers (control ).the smokers and nonsmokers divided to two group according to age. the ages were between (25-46)years.The results for liverfunction indicate a significant increase at(P<0.05)in Glutamate Oxaloacetae Transaminase(GOT),GlutamatePyruvate Transaminase(GPT) and Alkaline Phosphatase ( ALP) levels of smokers comparing with controlgroup in two age groups ,but non-significant differences at (P<0.05) in Serum Total Bilirubin (STB) ofsmokers comparing with control group, While the results for haematological parameters showed a significantincrease at(P<0.05) in white blood cells (WBC), red blood cells(RBC), hemoglobin(Hb) and Mean CellVolume(MCV)of smokers comparing with non-smokers in two age groups, but non-significant differencesat(P<0.05) in PLT of smokers comparing with non-smokers in two age groups. the results for blood pressureshowed a significant increase at(P<0.05) in in systolic and diastolic blood pressure in all age groups ofsmokers comparing with non-smokers.
Awareness of Creativity and Its Relationship to Speed and Accuracy of Futsal Soccer for Female Students Mohsen Mohamed Hassan1 , Nidaa Falih Hassan2 , Ali Muhammad Jawad Al-Sayegh3, Hayder Naji Habash Als
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11847

Abstract

Achieving success in the futsal soccer game also depends on the good potential in the accuracy and speedof implementing basic skills through the superior ability to accuracy and speed of passing the soccer (futsal)to the researchers as well as teachers and lecturers in this game within the scope of the lesson in the thirdand fourth stage, there is a weakness in most Female students in basic play skills, including the skill ofhandling, because they need to play to the speed and accuracy of performance and weakness, according tothe researchers ’opinion, lack of training on them requires awareness, thinking, focus and awareness that thegame needs physical and mental effort to master it, which the researchers decided to go into this problem, theresearchers used the curriculum Descriptive Basloubi survey and correlations because it fits with the natureof the research problem. The research community was determined by the students of the fourth stage in theCollege of Physical Education and Sports Science in the effectiveness of football halls for the academicseason 2018-2019 and the number (14) students, which were completely chosen for the experiment, in asimple random way ( Lottery) and by 100%. The researchers used the scale of creating awareness of theresearcher (Wahb Razzaq), where a scale consists of (28) items that the examiner answers according to fivealternatives, which are (always, sometimes, rarely, never) and grades are given (1,2,3,4,5) on Respectively,as the highest score on the scale was (140), the lowest score is (28), the name of the test - receipt, and eitherthe handover pass test toward players - the goal of the test - measuring the speed and accuracy of passingthem - the conclusions are - distinguished students of the Faculty of Physical Education and Sports Scienceat the University of Kufa In the fourth stage, a good reality of awareness and creativity also characterizedby a good reality in the performance and speed of the skill of handling the futsal football. There is a realcorrelation between the awareness of creativity and the speed and accuracy of the skill of handling futsalfootball. The researchers recommend that it is necessary to focus on psychological matters that increase thestudents ’motivation to think and awareness about creativity and help them in achieving self and forming apersonal sporting identity for each of them using encouraging educational methods to develop the skill ofspeed and accuracy of the passing skill of students in the College of Physical Education and Sports Sciencewhile practising practical lessons and performing sports skills Various specials, futsal soccer skills.
The Effect Esthetic Factors in Complete Dentures Wearers Acceptances Osama A. R. Alheeti 1 , Ghasak H. Jani2 , Jassim A. Al-E’nizy3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11848

Abstract

Objected: This essay has shown esthetics to be a salient factor for successful complete dentures. Completedenture esthetics can no longer be regarded as a method of tooth selection and arrangement alone or the colorsand contours of the denture bases and forms of the denture bases. Denture feel must likewise incorporatethose whole face for which those expressions from claiming inward feelings, personality, comfort, image,well-being, and recognitions about previous dental encounters need aid constantly on precise obvious. Boththose patients and the dental specialist must comprehend this interrelationship for ideal victory.Purpose: evaluate hard and soft components that contribute to Creating an effective finish successfulesthetical denture stylish rebuildingMaterial and Methods :The sample was selected from Baghdad University, College of Dentistry. A totalof 100 Iraqi adult Dental patients were clinically examined ( females and males). Each person was seatedin the Dental chair and was asked to look straight ahead and the measures have been taken according to thecase sheet prepared previously.Result : the results obtained from case sheet analysis using SPSS computer program (version 23) andshown significant statistic results in some criteria of determining aesthetic by facial measurement and othershown some unaesthetic result which may be due to clinical or laboratory error during the construction ofcomplete denture.Conclusion : A large portion of significant attention throughout finish denture development is thoserebuilding from claiming keep going feel. esthetic dives substantially further over the negligible placementof the teeth on the occlusal rims, size, shape, color about simulated teeth and denture bases need aid essentialOn the development of a stylish denture. Distinctive individuals have communicated different assumptionsfor settling on tastefully pleasing dentures.
Genotypic, Phenotypic Identification and Antibiogram Assay of Mrsa Isolated from Healthy Individuals in Wasit Province Haneen Saad Farhan1 , Zainab N. Al-Saadi2 , Jassim Fatehi Ali3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11849

Abstract

Staphylococcus aureus is a Gram-positive, facultative anaerobic, non-spore-forming, coagulase -positivebacterium. the Methicillin resistance is mostly mediated by the presence of mecA gene acquisition of mecAgene leads to altered penicillin binding protein (PBP), called PBP2a, with low binding affinity to methicillin.A total of 52 S. aureus strains were analysed. Antibiogram assay for 52 healthy carriers of MRSA isolates byusing Vitek 2 system to examine 16 different antibiotics.Methicillin resistance was confirmed by presenceof the mecA gene by PCR. Of 52 S. aureus samples, 52 isolates of them were MRSA confirmed by targetingmecA gene using PCR. Aims of this study detect methicillin resistant Staphylococcus aureus from nose ofhealthy individuals in Wasit province and confirming that by PCR to detect mecA Gene.
Study the Elevation of Fibrinogen level in Plasma Patients with Diabetes Mellitus Type 2 in Babylon Governorate Khawla A. Shemran
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11850

Abstract

Objective: The incidence of diabetes mellitus type 1 and type 2 has increased dramatically worldwide in thepast 20 years. Type 2 diabetes is probable to increase further in the future owing to the increase in obesityand the lack of exercise. This may rise the danger of unindustrialized cardiovascular illness in patientswith type 2 DM. It may be because fibrinogen has an important role in the increased risk of cardiovasculardisease. The aims of current study to find out the level of fibrinogen and its relationship to the level of bloodglucose in patients with type 2 DM. Methods: The study contain 50 patient with diabetes mellitus type 2 aspatient group and 50 adult apparently healthy as control group. All plasma samples were collected from allsubjects. The age for both groups concerning (43-75) years. The collected data include measures, plasmafibrinogen and HbA1c. Results: The present study revealed a significant increase in plasma fibrinogen,HbA1c concentration in patients compared with control group (p<0.01). Conclusion: The results of thisstudy reveal a significant increase in fibrinogen level in patients with diabetes mellitus type 2 withoutcardiovascular disease.

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