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INDONESIA
Indian Journal of Forensic Medicine & Toxicology
Published by Official Organ of Indian Association of Medico-Legal Expert
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Public Health
Arjuna Subject : Kedokteran - Epidemilogi
Articles 5,497 Documents
 156   157   158   159   160 
Correlation Ship between Some Hematological Parameters and Smoking for Students Manar Kareem Alquraishy1 , Alia Hussein Al-Mousawi1, Ahmed Adnan AL-Khafagi2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11885

Abstract

This study including collected 172 blood sample from AL-Kafeel University college, these samples including102 sample to male and 70 sample for female in which students samples were 53 for smoker and 119 nonsmoker , to evaluate some of parameters such as ABO , RH , blood pressure and clotting time .The results showed blood group A+ was more frequent which represent 33.7 % followed by O+ blood groupwhich represent 25.6% while B- was the last ,in other results displayed AB- and RH + was more frequent thanRH -. Systolic and diastolic pressure examined was demonstrated there were mild hypertension in bloodpressure between samples of studied, while result gloating time was exhibited range 30 sec to 3 minute .
Impact of Digoxin on Endocrine Glands in Male Rats Abbas A. Khudhair 1 , Kassim F. Abdulkareem2 , Muhsin S.G. Al_Mozie’l 3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11886

Abstract

This study is performed to provide the pathological changes of thyroid gland, pancreas and adrenal glandthat occurred due to exposure to the digoxin drug. The study uses twenty four adult male rat randomlydivided into 3 equal groups as follows: G1 control group treated with distilled water, G2 dosed with 0.3mg/kg digoxin and G3 dosed with 0.9mg/kg digoxin given orally by gavage daily for 60 days. All animals weresacrificed at the end of experiment and organs were collected in 10% buffered formalin for histopathologicalevaluation. Result reveals dilation of thyroid follicles with flattened follicular epithelium which increasewith high dose group digoxin in comparison with control. Adrenal gland showed moderate atrophy ofcortical layers especially zona reticularis with hyperplasia of medulla cells probably of digoxin like ouabainproducing cells, on the other hands pancreas doesn’t reveal remarkable changes except congestion of vesselsin high dose group compared to control.
Study Some Biochemical Parameters in Beta Thalassemia Major Patients in Males and Females Adults in Baghdad Rusul Yaareb Hameed1 , Sanad Baqir Mahammad2 , Bassam Francis Matti3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11887

Abstract

Our study aims to investigate in levels of Ferrtin and IL-23 in beta-thalassemia major patients (males andfemales), and compared with the control group (healthy individuals). The total number of samples were(88) blood samples, beta-thalassemia major patients (44) and healthy individuals (44). All the samplessubmitted to ELIZA test to determination Ferrtin and IL-23 in blood samples (males and females) (patientsand healthy group). According to our results, showed there are significant difference in and males with betathalassemia major and females with beta-thalassemia major in Ferrtin concentration as compared with thecontrol group at (P<0.01). In addition, Our study showed no there are significant difference in and maleswith beta-thalassemia major and females with beta-thalassemia major in IL-23 level as compared with thecontrol group at (P<0.01). Finally, there is a high increase in Ferrtin concentration of blood samples inmales and females, while there are little changes (non-significant) in IL-23 concentration of blood samplesin males and females.
Biological Activity of Complexes of Some Amino Acid: Review Lekaa K. Abdul Karem, Israa M. Radhi, Suhad S. Mohammed
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11888

Abstract

Schiff bases (SBs) based on amino acid derivative stand for multipurpose ligands that formed by condensingamino acids with carbonyl groups. They are significant in pharmaceutical and medical areas due to theirwidespread biological actions such as antiseptic, antifungal, along with antitumor actions. Transitionmetallic complexes resulting from SB ligands with biological activity were extensively experimented in theliterature. In this article, we review, in details, about synthesizing and biological performances of SBs alongwith its complexes.
Measuring of Interleukin -22 and IL -17a Levels in Seropositive and Seronegative Rheumatoid Arthritis Patients Suhad Hassan Aubaid1 , Ekhlas Saddam Falih2 , Zeina Anwar Jaffar3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11894

Abstract

Background: “Rheumatoid arthritis (RA) is an inflammatory disease leading to joint disruption”. Theinstant study designed to detect serum markers in RA that could discriminate between seropositive (SP) andseronegative (SN) by evaluating levels of IL- 22and IL -17a in patients with rheumatoid arthritis. Methods:In this cross-sectional study, a total of sixty Rheumatoid arthritis (RA ) patients’ age and sex-matched withhealthy controls were involved in the present study. The serum IL- -22 and IL -17a levels were measuredusing an ELISA kit. Results: The mean ±SD age in seropositive and seronegative was (37.22± 11.29 and34.28±20.3 years, respectively), while in control group was (27.14±9.33 years). Furthermore, serum IL- 22and IL -17a level was significantly higher in seropositive and seronegative RA patients compared to healthycontrols (P<0.001). There was no significant variation in serum IL -22 and IL -17a level according to theseropositive and seronegative in RA patients (p>0.05), but there was a positive correlation between themROC test representing a highly sensitive and specific . Conclusion: The present study exhibited higherserum IL- 22 and IL -17a levels in seropositive and seronegative in RA patients compared to healthy controls.Therefore, IL- 22 and IL -17a can be considered as a biomarker for RA disease, with high sensitivity andspecificity but these cytokines couldn’t be used as discriminated between SP and SN in RA patients.
Detection of B1 Gene in Toxoplasma Gondii and the Role of Interleukin 2 in Abortive Women Infected by this Parasite Hanan Mohammed Saied Jassam1 , Thaer Abdulqader Salih2 1 Post graduate/Department of Biology/ Colleg
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11895

Abstract

The study included 80 blood and serum samples of aborted women with Toxoplasma gondii infectionand healthy, uninfected and non-aborted women in the control group. For the period from August-2018to June-2019, in collaboration with the Falluja teaching hospital for women and children and the Ramaditeaching hospital for women and children, the Enzyme-Linked Immunosorbent Assay (ELISA), also to theToxoplasma gondii (B1) gene, the Nested polymerase chain reaction technique are used. The results of thestudy showed by that Elisa Toxoplasma gondii, IgG infection (50%), IgM infection (12.5%), and IgG & IgMInfection (12.5%), were mean (108.16±47.02) (99.02±31.47) (96.45±20.68) respectively compared with thecontrol group (25%) (0.94±0.93) and Interleukin-2 shown in IgG group (48.66±12.01) and control group(14.28±6.84) decreased compared to IgM (81.19±9.66), and IgG & IgM (80.71±9.18) group. Nonetheless,using nested PCR, the B1 gene showed just (12.5%) of all study samples in IgM and IgG&IgM alone andall IgG negativly
Bacteriocin Production in Bacillus cereus Food Isolates with Molecular Detection of cerA gene
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11897

Abstract

Sixty-three Bacillus cereus strains isolated from food samples. All strains were subjected to DNAsequencing for 16S rRNA for identification. 15 strains were registered at GenBank of NCBI and givennew accession numbers. 41.26% of the isolates showed bacteriocinogenicproduction activity against fourbacterial speciesviz,Bacillus cereus, Staphylococcus aureus, Escherichia coli and Salmonella sp. Presenceof cerA gene coding for cericidine(a type of bacteriocin) was detected in 7.69% of the isolates that producedbacteriocin. Bacterionwas precipitated by ammonium sulphate and purified by dialysis. Antimicrobialactivity of precipitated bacteriocin against the four types of bacteria showed the effect on Bacillus cereus andStaphylococcus aureus growth but not on Escherichia coli and Salmonella spp.The produced bacteriocinhas a molecular weight ranging from 47-54 KDa.Estimation of the concentration and physical and chemicalproperties of bacteriocin were also investigated.
The Diagnostic Efficacy of Visually Enhanced Lesion Scope (Velscope) In Identifying Benign , Dysplastic and Cancerous Oral Lesions Suroor Ali Jabbar1 , Fawaz Aswad2 , Ameena Ryhan. Diajil3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11898

Abstract

Background : Visual inspection by conventional oral examination (COE) has been the backbone of oralcancer and precancer detection. More recently, several commercially available diagnostic adjuncts havebeen developed to aid clinicians in the early detection of oral epithelial dysplasia (OED) and squamous cellcarcinoma (SCC), such as OralCDx, Toluidine coloring, ViziLite machine, Identafi machine, and VELscope.VELscope is a technology based on the principles of autofluorescence mechanism imaging. This deviceoffers in-vivo, real-time, direct visualization of tissue autofluorescence, termed direct visual fluorescentexamination (DVFE). It is currently marketed as an oral cancer screening tool to be used with all new andrecall dental patients and as an aid for surgeons in tumor margin delineation.Aim of the study :was to obtain auto fluorescent data on variety of histopathologically distinct oral lesionsand assessment of direct auto fluorescent examination validity in identifying dysplastic (premalignant)and malignant oral mucosal lesions using VELscope and judgment with histopathological examination.Materials and methods: Routine oral examination of fifty patients having suspicious oral lesions followedby direct autofluorescent examination by VElscope and then histopathological examination.Conclusion : VELscope can be considered as an adjunctive device to enhance the visualization of oralmucosal abnormalities, but not as a tool for risk stratification.
Test Some New Media for Cultivation of Gram Positive and Gram Negative Bacteria Firas Nabeeh Jaafar1 , Wala’a Shawkat Ali2 , Nadia Kamil Bashar1 , Rayim Sabah Abbood1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11899

Abstract

Possibility of using the wastes of used paper to prepare a culture medium for bacterial growth was carriedout in this study, five experimental culture media (paper waste extract agar, glucose paper waste extract agar,yeast extract paper waste extract agar, NaCl paper waste extract agar and GYN paper waste extract agar)that made from the extract of used papers were prepared to cultivation two species of Gram positive bacteria(Staphylococcus aureus and Streptococcus spp.) and two species of Gram negative bacteria (Escherichiacoli and Pseudomonas aeruginosa) in comparison with readymade media (nutrient agar and brain-heartinfusion agar). Heavy growth of bacteria was appeared on yeast extract paper waste extract agar and GYNpaper waste extract agar media, while no growth was happen on the other three experimental culture media.The results of statistical analysis found that there is no significant different at P?0.05, using LSD test amongyeast extract paper waste extract agar , GYN paper waste extract agar and nutrient agar or brain-heartinfusion agar media .These results indicate likelihood of using two experimental culture media instead ofnutrient agar or brain-heart infusion agar to cultivation of bacteria.
Blood Lymphocytes Detection in Iraqi Diabetic Type 2 Patients Infected with Chronic Toxoplasmosis by Using Flow Cytometry Israa Kasim Al-Aubaidi1 , Sarah Ali Saeed1 , Ahmed Issa Jaaffar2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11900

Abstract

Toxoplasma gondii is an obligate parasite infects different species of mammals as intermediate hosts whileinfects number of family of cats as definitive hosts. It has three morphological types: tachyzoites, bradyzoitesand sporozoites. It causes (H.I.) humoral immunity and (C.M.I) cell mediated immunity response which ismeasured one of the most characteristic immunological types of this parasite infestation. Diabetes mellitus(DM) is considered as a heterogeneous group of metabolic abnormalities with hyperglycemia due either toa reduction of insulin biological function or absolute insulin deficiency. Researchers discovered that longterm infection with T. gondii may be dangerous agent for DM type 2, and there is no relation betweentoxoplasmosis and DM type 1. The aim of this study include detection of CD3 in Iraqi diabetic type 2infected with chronic toxoplasmosis by using flow cytometry. This study included 100 blood samples werecollected from Iraqi diabetic type 2 patients and 50 blood samples of healthy volunteers with age range(15-75) years and mean 49.9±12.8 from Al – Imam Ali general hospital during May 2018 until August inthe same year. Diagnose of toxoplasmosis infection was done by using rapid diagnosis test/ cassette and byToxo IgG antibodies immulite torch assay while diabetes diagnosis by fasting glucose tests in addition CD3were detected by using flow cytometric method. Diabetic patients a group infected with toxoplasmosis havethe highest level of blood blood sugar in comparison with other groups. Forty – five samples of diabetictype 2 infected with chronic toxoplasmosis by detection IgG antibody in highly level 106.2± 90.2 withhighly significant differences in comparisons with other groups ( diabetic patients 3.6± 1.2 and control group4.0±0.6 ) while any results for IgM Abs didn’t found in this study. Seventy – five samples studied in flowcytometry method which include twenty – five samples for each group. Diabetic patients with toxoplasmosisrecorded highest CD3 its mean 30.7± 14.51 pg /ml flowed by diabetic patients group has 24.84±9.47 pg /mland control group has 20.03± 9.42 pg /ml with significant differences.

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