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INDONESIA
Indian Journal of Forensic Medicine & Toxicology
Published by Official Organ of Indian Association of Medico-Legal Expert
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Public Health
Arjuna Subject : Kedokteran - Epidemilogi
Articles 5,497 Documents
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Deleted Anwar A. Abdulla
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12020

Abstract

a
Sensitive Simultaneous Estimation of Atorvastatin Ca in Pure and Dosage Forms Via Developed CFIA using 1,2 Naphthoquinone-4-Sulfonate as a Suitable Organic Agent Bushra B. Qassim1 , Asmaa A. Zydan2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12021

Abstract

Objectives: A sensitive visible spectrophotometric method and FIA/merging zones technique was developedfor the determination of atorvastatin calcium in pure material and tablet dosage form.Methods: Atorvastatin calcium has a free carboxylic moiety in its structure, which when being deprotonatedin basic medium facilitates associated the reagent with the drug. This method was based on the formationof red colored chromogen of drug with 1,2-Naphthaquinone-4-sulfonate(NQS) in basic medium (NaOH).The absorbance of the chromogens was measured at their respective wavelengths of maximum absorbanceagainst the corresponding reagent blankResults: The red colored product is directly completed in basic medium and exhibits maximum absorptionat 525 nm. Different factors affecting the formation of the product and optimized in order to obtain the bestconditions for the experiment and its stability were studied. Method validation was done over a concentrationrange of 2-10 and 1-20 ?g/mL for batch and FIA method respectively.
Gall Bladder Wall Thickness: Sonographic Accuracy and Laparoscopic Cholecystectomy Conversion Rate, Evaluated by Histopathology Mohammad Faraj Dawod Al. Jafary1 , Ammar Fouad Abdulrazaq2 , Ayman Nadeem Kadum3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12022

Abstract

Background: Preoperative prediction of a difficult laparoscopic cholecystectomy can help the patient as wellas the surgeon to prepare better for intraoperative risk and the risk of conversion to open Cholecystectomy.Aim of study: Evaluation the impact of gall bladder wall thickness, on the outcome of laparoscopiccholecystectomy and conversion rate to open cholecystectomy assessed by sonography preoperative andpostoperative measurement of gall bladder wall thickness by histopathology.Patients and Methods: A prospective study conducted in the surgical unit, Department of surgery, BaghdadTeaching Hospital between November 2010 and November 2011. Abdominal Sonography performed in 110consecutive patients before laparoscopic cholecystectomy. The surgeon re-verified sonographic finding inoperative room, and postoperatively, the gall bladder specimens were sent for histopathological measurementof wall thickness.Results: Out of 110 patients with cholecystolithiasis on sonography, we encountered easy laparoscopiccholecystectomy in 80 patients (72.7%), difficult laparoscopic cholecystectomy in 24 (21.8%) and theprocedure was converted to open cholecystectomy in six patients (5.5%). The difference between Sonographicand histological measurement was within 1 mm in 102 patients (92.7%), and the other 8 patients was with1.5 mm (7.3%) with sensitivity of (100%), specificity of (83.3%) and accuracy of (97%).Conclusion: An accurate preoperative diagnostic sonography is mandatory for planned laparoscopicgall bladder surgery to provide information for the selection of the most appropriate approach and avoidintraoperative difficulties and surprises. On sonography gall bladder wall thickening is the most sensitiveindicator of technical difficulties during laparoscopic cholecystectomy. Such difficulties may requireconversion to laparotomy.
Evaluating the Effect of Addition of Titanium Dioxide Nanoparticle on Some Physical Properties of Flowable Composite Resin Sazgar Srbast Qasim1 , Diyar Khalid Bakr2 , Dara H. Rasheed3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12023

Abstract

Objective: The flowable dental composite resins were introduced to the dental specialty because of theadvantages they possess over the conventional composite resins. The use of nanotechnology in the dentistryfield is one of the growing innovations in recent years. The aim of the present study was to evaluate certainphysical properties of flowable dental composite after incorporation of titanium dioxide nanoparticles(TiO2NPs).Materials and Methods: In the present study, TiO2NPs at 1.25 % and 2.5% concentrations were added toflowable composite, while the unmodified composite was used as control. Then the physical properties of thecontrol and modified composite resins, including flowablity, radiopacity and water sorption and solubilitywere tested. Data were analyzed with One way ANOVA, using SPSS 20.Results: The results showed that there was statistically significant difference among the tested groupsregarding flowability and radiopacity (P<0.05). In addition, there was no significant difference amongcontrol group and TiO2 modified groups regarding water sorption and solubility.Conclusion: Based on the results of the present study, a flowable dental composite was successfullyreinforced with TiO2. Incorporation of small weight percentages of this nanofiller exhibited propertiessimilar to the control material regarding water sorption and solubility. The flowability was slightly reducedand radiopacity of the reinforced composites was increased, these changes were acceptable for clinicalapplications and below ISO standards limits.
Antimicrobial Activities of Green Biosynthesized Iron Oxide Nanoparticles Using F. Carica Fruit Extract Nada Khudair Abbas1 , Azhar A. F. Al- Attraqchi2 , Jenan Hussien Taha3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12024

Abstract

In the current study, iron oxide nanoparticles (IO NPs) were synthesized via modified green synthesistechnique using F. carica fig extract as reducing agent. Furthermore, the microstructural propertiesof the synthesized IO NPs have been thoroughly elucidated. In details, the acquired NPs diameter wasfound to be in the range of 11-29 nm and of root mean square (RMS) of 0.64 nm using TEM and AFMtechniques, respectively. Consequently, the antifungal and antibacterial activities of the synthesized IO NPswere screened against Candida and Aspergillus species as well as Gram-positive Staphylococcus aureusand Gram-negative Acinetobacter species, respectively. The presented IO NPs play an active role in theantimicrobial activities evidencing the well-organized materials system for biomedical applications.
Molecular Detection of Antibiotics Resistance Genes in Burkholderia cepecia isolated from Diabetic foot infection Maryam Riyadh Al-Muhanna1 , Mahdi Al-Ammar2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12025

Abstract

This study aimed to isolate and diagnose Burkholderia cepetia from clinical specimens of diabetic foot andstudy the resistance of bacteria to antimicrobial agent in Najaf governorate from Augest 2019 to November2019, which includes 120 clinical specimens for both sexes with an age ranged between )35-70) yearsold. The diagnosis of bacteria isolates was based on microscopy, as well as the culture and biochemicalcharacteristics as an initial diagnosis. The final diagnosis by the Vitek-2 compact system .Burkholderia cepacia 8(6.6%). Antibiotic sensitivity test was examined by dick diffution method,Burkholderia cepacia isolates showed high level of resistance almost for all ?-lactam antibiotic classesunder study which included;, ceftriaxone, cefoxitin and Cefepime, with percentage of (100%); ticarcllinwith clavulalnic acide ,piperacllin , ceftazidime, tobramycine, ciproflaxcin and levofloxacin with percentageof (87.5%); aztreonam and amikacine with percentage(62.5); meropenem , imipenem and gentiamycin withpercentage of (37.5%).At molecular study, the investigated the presence of antibiotic-resistant genes (blaImp, blaOxa,blaKpc,blaCTX-M) using PCR technique and electrophoresis systems. 6/8 Burkholderia cepacia isolateswere with blaOXA and (6/8) of isolates carry blaCTX-M gene, all isolates of Burkholderia cepacia gavenegative result of blaIMP and blaKPC gene. Finally PCR analysis showed that the integron gene was (3/8%).
Evaluation the Antimicrobial Effect of Glycerin Magnesia on Some Bacteria, In-Vitro Study Maher Saber Owain1 , Khalid Ismael Oleiwi2 , Ali Ghazi Atiyah1 , Mustafa Salah Hasan2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12026

Abstract

This study was aimed to assess the efficacy of glycerin magnesia on some bacteria. A thirty percent of glycerinmagnesia were prepared as explained below. Many types of bacteria including Proteus spp., Staphylococcusaureus, Staphylococcus epidermidis, Acinetobacter, E. coli, Pseudomonas aeruginosa, Salmonella spp. wereselected for this study and obtained from university of Tikrit, college of veterinary medicine. A bacterial brothwere prepared, a then a sterile swab were emulsify in these broth and streaked on muller hinton agar plateand allowed till dry, then a holes were filled with a given glycerin magnesia and incubated for 24hrs. at 37?C. The results showed that a higher antibacterial effects of glycerin magnesia against Staphylococcus aureusfollowed by Proteus spp., Pseudomonas aeruginosa, Salmonella spp., Acinitobacter, E. coli, Staphylococcusepidermidis respectively.In conclusion, the glycerin magnesia have a wide range antibacterial effect and can be used in future inwound healing.
Prevalence of Histopathological Diagnosis of Benign and Malignant Breast Lesions in Al-Muthanna Province for Two Years Duration Sarah Ali Abed1 ; Ali Adnan Jabbar2 ; Roaa Salih Mahdi3 ; Ali Mohammed Khaleel4 ; Suha Suhiel Hussei
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12027

Abstract

Introduction: Breast cancer (BC) is a common form of cancer among women globally. It is considered 5thcause of death in females with equally to 522,000 patients / year. Nowadays it is considered 15.4% of deathin developed countries after lung cancer. In developing nations, breast cancer considered the 1st cause ofdeath for females 324,000 cases of deaths (14.3%) for total deaths. Method: One hundred and six femalepatients with breast lesion were collected from the Al-Hussein Teaching Hospital Laboratory/Histopathologydepartment –Al-Muthanna province, during the period from January 2018 to January 2020. The data forcases were collected to study the age, type of breast lesion whether benign or malignant. Haematoxylin/Eosin staining done in Al-Hussein Teaching Hospital Laboratory / Histopathology department. Results:Cross sectional study for 106 patients done in Al- Muthanna proven for assessment breast mass and identifymalignant and benign lesion, mean age of patients was (39.4 ± 15.8) years old, with min age 13 years andmax age was 81 years old. Types of biopsy taken from surgeon were 71% excisional biopsy, 21% mastectomyand 8% true cut biopsy. After pathological assessment of biopsies showed 51% malignant and 49% benign.In addition, this assessment distributed as following: fibroadenoma 31.1%, IDC/ grade II 29.2%, IDC/ gradeIII 9.4%, ILC and fibrocystic changes 5.7% and IDC grade I 3.8% and other types of malignant and benignafter pathological assessment. Significant association between age groups and cancer of breast. Conclusion:After pathological assessment of biopsies, malignant breast cancer (IDC/ grade II, IDC/ grade III, ILC andfibrocystic changes) most common changes, significant association between age group and breast cancerdevelopment more age group 41- 50 years old and then (31- 40), (51– 60) years old respectively.
A Histological and Histochemical Study on Olfactory Bulbs to Detection Amyloid Protein Depositions by Congo-Red and Routine Staining Techniques Rajaa Ali Moheiseen Al- Taee1 , Muna Hussain Al-Aameli2 , Yarub Modhar Al-Qazwini3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12028

Abstract

In this study, histological and histochemical techniques were used to examine olfactory bulbs in the albinorat male. Thirty male albino rats were split into three age groups (10 animals each) in the current research:Group I: consider as a control group, including adult animals aged 3 months .Group II: include animalsaged 6 months. Group III: include animals aged 12 months. The histological architecture of the layersof olfactory bulbs and their main cells was identified by using H & E staining techniques, meanwhile thecomposition of each layer in albino rat was evident. In the glomerular and mitral cell layer of group III,olfactory bulbs showed reduced neural density. Modified staining with Congo - red was conducted forhistochemical studies. Compacted amyloid cores were found in group III animals’ olfactory bulbs, whiledispersed amyloid cores were found in group II&III olfactory bulbs’ cortex. The present study adds to ourknowledge of the impact of amyloid protein on olfactory bulbs and their prospective neurodegenerationinvolvement.
The Intraperitoneal-Ketoprofen-Histopathological Induced Alterations in the Wistar Rat Kidneys Samer Riyadh Fadhil , Hakim Bahlok Jebur2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12029

Abstract

The current-focused study was carried out to generate a data profile about expected changes that could beinduced in the kidneys of rats due to the use of intraperitoneal ketoprofen (KP). The study involved using24 adult male Wistar rats sorted randomly into four groups (six animals per group). One group was treatedas a control group, C, which was supplied with distilled water (DW) only. The remaining animals werepresented as (50KP), (25KP), or (12.5KP) groups that received 50mg/kg.b.w., 25mg/kg.b.w., or 12.5mg/kg.b.w., respectively, of KP. The experiment was continued for 70 days, and the kidney tissue sampleswere collected from the scarified animals at the end of day 70 of that experiment. The kidney tissues of the50KP animals revealed dilation of the tubules throughout the outer strip of the outer medulla with necrosisand sloughing of the epithelial cells of the proximal convoluted tubules (PCTs). However, the 25KP groupsuffered lesser grades of epithelial-cell sloughing of the PCTs with lower levels of dilation of tubules thanthose recorded in the 50KP group. On the other hand, the kidney tissues of the 12.5KP group showed onlydilation in the PCTs. The present experimental data unveil the side effects generated by the use of theintraperitoneal KP in the examined rat kidneys which should be used as a launching set of information forbetter use or further study this drug and its side effects in human patients.

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