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INDONESIA
Indian Journal of Forensic Medicine & Toxicology
Published by Official Organ of Indian Association of Medico-Legal Expert
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Public Health
Arjuna Subject : Kedokteran - Epidemilogi
Articles 5,497 Documents
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Epigallocathecingallate (EGCG) Antifungal Properties for Candida Isolates from HIV/AIDS Patients with Oral Candidiasis in Compare with Fluconazole Dwi Murtiastutik1 , Cita Rosita Sigit Prakoswa2 , Indah Setyawati Tantular3 , Yusuf Wibisono1 , Afif
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13549

Abstract

Background: Oral Candidiasis (OC) still mainly oportunistic infection problem in HIV/AIDS Patients.Due to increasing report of fluconazole resistant as common antifungal drugs nowadays, there have beenmany studies focusing on natural substances and its antifungal properties. In this study, a form of green teaextract, named Epigallocathechingallate (EGCG) 1,25% were examined for their in vitro antifungal activity against Candida sp in comparison to fluconazole (2 mg/ml) as standard antifungal agents.Objective:To evaluate the antifungal activity of EGCG in compare with fluconazole against Candida isolates takenfrom HIV / AIDS patients with OC. Methods: Fourty Candida sp. isolates taken from HIV / AIDS patientswith OC in the Outpatient Unit and Inpatient Installation of the Infectious Disease Intermediate Care Unit(UPIPI) Dr. Soetomo, Surabaya. Antifungal activity were evaluated by using microdilution tests. Results:The microdilution test revealed the MIC of EGCG for all Candida sp. was 0.625%, while the MIC offluconazole was 100% for all Candida sp. There was significant difference (p <0.05) between the MICvalues for Candida sp. by fluconazole and EGCG. The MFC values of EGCG was 50%, while value offluconazole MFC was 100%. Conclusion: Antifungal activity of EGCG with fungistatic and fungicidaleffect is better than fluconazole.
The Significance of CD4 to the Number of Grown Candida Colonies in Oral Candidiasis Patients with HIV / AIDS Dwi Murtiastutik1 , Cita Rosita Sigit Prakoeswa1 , Indah Setyawati Tantular2,3
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13550

Abstract

Basic data of this study showing isolates mostlytaken from male than female (77.5% and 22.5%). Themost age group is productive age groups in the agerange of 26-35 years with 20 subjects (50%). In 2017report of the Directorate General of Disease Controland Environmental Health, Ministry of Health Republicof Indonesia, found HIV/AIDS patients more malethan women, and related more common in young adultwhich makes it more likely to engage in unsafe sexualbehavior that is at risk of HIV transmission (8). Thedomicile of HIV / AIDS patients with OC mostly camefrom Surabaya (90%). This is because most patientshave to seek help from the nearest health center withmost patients from within the city. The isolates mostwere taken from HIV / AIDS patients with OC who hadan absolute CD4 count <100 cells / L for 28 patients(70%). This data is supported by a 2015 Indian study byKumar that showed 71.4% of patients with a CD4 cellcount <200 cells / µL obtained by the fungus growth ofCandida species from OC lesions (9).
Deleted Dwi Murtiastutik1 , Cita Rosita Sigit Prakoeswa2, Indah Setyawati Tantular3
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13551

Abstract

l
Impact of Bone Marrow Mesenchymal Stem Cells on Different Body Organs of Albino Rats Exposed To Cadmium Chloride Toxicity: A Systematic Review Elham H. Ahmed 1, Mohammed E. Grawish2 , Mohamed Abdulrahman3 , Menatalla M. Elhindawy4 , Mohamad E.
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13552

Abstract

Background and Objective: Cadmium chloride (Cd cl2) is a highly toxic heavy metal that causes severedegenerative effects to different body organs. Bone marrow mesenchymal stem cells (BMMSCs) may havea protective effect upon CdCl2 induced structural and functional changes in the rat’s organs. This systematicreview aimed to test the regenerative effects of bone marrow?derived mesenchymal stem cells on differentbody organs of albino rats exposed to cadmium chloride toxicity.Methods: Experimental animal studies were identified by the following electronic databases: PubMed,Scopus, Ovid Medline, KoreaMed, Google Scholar, Latin American and Caribbean Health SciencesLiterature (LILACS) and Central Cochrane Library. The literature search was done from January 2010 to2020.Conclusion: From the selected articles, using stem cell therapy enhances the regeneration process in CdCl2toxicity of different body organs.
Diagnostic Accuracy of Recto-Sigmoid Index Evaluation on Barium Enema in Hirchsprung’s Disease Emma Ratna Fury1 , Andy Darma2 , Alpha Fardah Athiyyah3
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13553

Abstract

Background: Hirschsprung’s disease is a congenital disease caused by the absence of ganglion cellsin the bowel wall, which results in functional obstruction of the aganglionic segment due to failure ofrelaxation during peristalsis. The recto-sigmoid index on barium enema may constitute in the diagnosis ofHirschsprung’s disease.Aim: To evaluate the sensitivity, specificity, positive predictive value, and negative predictive value ofrecto-sigmoid index for the diagnosis of Hirschsprung’s disease in patients who underwent barium enemaexamination.Methods: This retrospective study was conducted in Dr Soetomo Hospital. Medical records of patientssuspected of Hirscsprung’s disease who underwent both barium enema examination and diagnostic fullthickness biopsy were evaluated. Moreover, the sensitivity, specificity, positive predictive value (PPV), andnegative predictive value (NPV) of recto-sigmoid index was calculated.Results: A total of 37 patients were examined; 21 of them were male. The average age was 24.5 (SD 17.34,range 1 month-72 month). Biopsy revealed the absence of ganglion cells in 32 (91,8%) patients. The rectosigmoid index agreed with the histopathologic diagnosis in 30 (81%.) patients. The sensitivity, specificity,PPV, and NPV were 87.5%, 40%, 90.5% and 33% respectively.Conclusion: Recto-sigmoid index evaluation in barium enema for the diagnosis of Hirscsprung’s diseasehas poor diagnostic accuracy.
Edible Oils Consumption and the Fatty Acids Profile of the Most Common Consumed Brands in Shiraz, Iran Erfaneh Aalipanah1, 2, Enayat Berizi1 , Anoush Khamoushi1, 2, Morteza Zare1 , Zahra Sohrabi1
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13554

Abstract

Aim: This study aimed to measure edible oils consumption, the most preferred brands and their fatty acidprofile compared with standard values in Shiraz, Iran.Methods: In this cross-sectional study 384 households were assessed. Edible oil types (frying, sunflower,corn, canola, rapeseed, mixed, solid, olive, grapeseed, sesame, and animal), brands, volume of consumed,attention to the label, and changes in the volume of oil consumption compared to the last year were measured.Resistance, fatty acid patterns, total trans fatty acid, smoke point, and saturated fatty acids of the three mostfrequently consumed edible oils were compared to the standard recommended values.Findings: The mean of households? oil consumption was 40.34 ± 32.88 kg per year. Frying (78.6%),sunflower (60.70%), and olive (37.5%) oils were the most common consumed oil. 57.6% of participants readthe labels. 28.9% could not understand the labels information. Compare to the last year, 53.8%, 46.8%, and55.5% of studied households reported decrease in the liquid, solid and frying oils consumption. Fatty acidprofile in majority of studied brands were in the standard range. Myristic acid level in the three major brandsof solid oil was out of the standard range. The level of C16:1, C18:1, C18:2, C18:3 in the third commonolive oil, C14:0 and saturated fatty acid in the third common frying oil were out of the standard range. Theresistance level in the third common corn oil, the first common and the third common frying oil was out ofthe standard range.Conclusion: Liquid, solid and frying oils consumption among studied population decrease than the lastyear, which can be helpful to reduce the risk of chronic diseases. The fatty acids profiles of the most commonconsumed brands were in the standard range.
Mitigation of the Biofilm Using Nanoparticles By Enhanced by D-alanine and D-proline Fakhri Alajeeli
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13556

Abstract

The present study was undertaken to investigate the susceptibility patterns as antifungal and growth of certainD-amino acids inhibitory effects, includes D-alanine(D-ala) and D-proline (D-pro). The obtained resultsindicated that D- proline acid is potent mostly as antifungal, among tested D-amino acids, then followedby D-ala. The study aim was evaluating the of D-amino acids and nanoparticles against Candida albicansadhered cells and bio-films. Results showed that D-ala MIC value was 25 ?g/ml, while in methods of TCP,(D-pro) MIC 50 ?g/ml value. Nanoparticles (lithium and silver) and amino acids (D-pro and D-ala) effectin preventing cells adhesion on polystyrene surface and mature bio-film inhibition was studied. Highestinhibition was obtained at D-pro concentration 50 ?g /ml, while the lowest at concentration D-ala 25 ?g /mlagainst mature bio-film and cell adhesion.
Detection the Serogroups of Escherichia coli isolates from Urine sample of UTI patients Fakhri S.Ahmed Ph.D
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13557

Abstract

Aims: Determining of E.coli serogroups which isolation from UTI patientsPatients and Methods: the study was conducted between September 2019 to April 2020 and they wascollected 40 sample from patient how are diagnosis with UTI during this study .Results: From the UTI patients individuals, 40 strains were identified in which O serogroups showed8(30%) and 2 (20%), respectively, of occurrence in those strains. Moreover, the enterohemorrhagic E. coli(O157 H7) and the enteroaggregative E. coli (O44 H7) were detected in two strains of the UTI-based urinesamples only. For the antibiotic resistance properties of the biofilm positive producers isolated from the UTIurine samples, multidrug resistance was revealed in 60% of those isolates; however, only 20% of the biofilmnegative producers from the UTI urine samples showed the MDR characteristics. Biofilm was identified in75% of the UTI urine. For the antibiotic sensitivity test, the results demonstrated that the sensitivity rates toImipenem, Amikaci, gentamicin, and tobramycin were 90%, 100%, 65%, and 40%, respectively, in the UTIstrains.Conclusion: The herein study provides thorough data about the Escherichia coli based biofilm,
Management of Non-Vital Teeth Discoloration with the Internal Bleaching : A Case Report Dewi Fitria Anugrahati1 , Rizky Ernawati1 , Windi Irsya1 , Yulianti Kartini Sumur1, Sukaton2 , Kun I
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13558

Abstract

Tooth discoloration due to intra coronal bleeding after trauma can cause aesthetic disturbances andappearance. Internal bleaching is a non-invasive method to restore tooth color which is performed afterendodontic treatment by placing a strong oxidizing agent in the pulp chamber. Aim of this study was toreport the success of internal bleaching procedure to restore discolored teeth due to trauma. A 44-year-oldfemale came to Conservative Dentistry Universitas Airlangga with chief complaint of the upper right insisortooth looks darker than the adjacent teeth. The patient had fallen 10 years ago from her motorcycle and hertooth had been painful and then the pain disappeared. In intra oral clinical examination, it showed that tooth11 had discoloured, vitality test of tooth 11 showed no response to electric pulp test. Radiographic viewshowed that radiolucen in periapical area and no fracture was found. At the first visit, treatment of singlevisit root canal using rotary instrument and gave dressing calcium hydroxide. A week later on the secondvisit, internal bleaching with hydrogen peroxide 35% was done. After bleaching, calcium hydroxide wasapplied to neutralize the oxidizing agent and then re-evaluated for the color. At the third visit, it showed thatthe tooth colour was obtain as desire from C3 to A3 (Vitapan Classical). The internal bleaching procedureon the traumatized tooth was the best option to restore the function and aesthetics according to the originaltooth colour and also provided benefits in terms of saving time, lower price, and maximum results.
The Relationship between Antithyroid Antibody and Pregnancy Outcome in Invitro Fertilization and Embryo Transfer Fawz Alaa alikhan1 , Azhar Mousa AL-Turiahi, 2 Asceel Hassan Bader3
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13559

Abstract

Background of the study: One type of assisted reproductive technique is intracytoplasmic sperm injection(ICSI) Despite use ofintracytoplasmic sperm injectionICSI it remain has low birth rate due to failurerate there are many markers associated with poor predict the outcome one of these factor effect outcomeinintracytoplasmic sperm injection ICSI presence of antithyroid antibody ineuthyroid patientAim of study: To examine the effect of antithyroid antibody on pregnancy results following the in vitrofertilization and fetus transmitMethods and patients : Cross section study conducted at AL-Sader infertility center atotal of eighty oneIraqi women were enrolled in this study of them 22positive antithyroid antibody,59 negative antithyroidantibody were undergone intra-cytoplasmic sperm injection ICSI in Al-Sader infertility center. The group ofthe study involved onlyeuthyroid women outwardly any clinically showed autoimmune illness.Result:there was higher significant difference p value<0.004 in fertilization rate number of availableembryos p value<0.003 and number of transfer embryos p value< 0.001among study group which washigher in group of women with antithyroid negative followed by,group with antithyroid positive in infertileeuthyroid women Conclusion:patients with antithyroid antibody associated with poor pregnancy outcomein infertile euthyroid women undergoingintracytoplasmic sperm injection ICSI

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