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INDONESIA
Indian Journal of Forensic Medicine & Toxicology
Published by Official Organ of Indian Association of Medico-Legal Expert
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Public Health
Arjuna Subject : Kedokteran - Epidemilogi
Articles 5,497 Documents
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Molecular Detection of Pseudomonas aeruginosa and its Relationship with Multidrug Resistance and Transposons Wathiq Abbas Hatite Al-Daraghi1, Mohammed Sattar Abdulkadim Al-Badrwi1, Haider Rida Jassim1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2819

Abstract

P. aeruginosa is currently one of the most frequently nosocomial pathogens and the infections due to this organism are often difficult to treat due to antibiotic resistance. P. aeruginosa is an important pathogen in hospitalized patient’s causative to their morbidity and mortality due to its multiple resistance mechanisms. Therefore, as a therapeutic option becomes restricted, the search for a new agent is a priority. One hundred and fifty samples were collected from different sources, divided into two main groups: clinical (80)samples and (70) hospital environment samples as a Nosocomial, collected all from October to the December of the year 2018. All of these samples were cultured by specific and differential media, Forty (40) isolates of P.aeruginosa bacteria were identified by using microscopic examination, biochemical tests. The identification of 40 isolates of P.aeruginosa bacteria confirmed by VITEK-2 system. A molecular detection the presence of Tnp-R gene in P. aeruginosa bacteria by conventional PCR to detect the Transposons and their relationship with multiple resistance of bacteria.
Antifungal Activity of Silver Nanoparticles Using Penicillium Chrysogenum Extract Against The Formation of Biofilm for Candida Glabrata Nada S. Salah1, Thamer A.A. Muhsen1, Mohssen H. Risan2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2820

Abstract

The results showed that 71 isolates of Candida spp were isolated from patients with leukemia both women and men, Isolate 59 from C. glabrata while the number of isolates of C.albicans, C.tropicalis, C.krusei and C.kefyer were 6,3,2,1 respectively. The size of the nanoparticle was measured using AFM, The highest peak was 455 nm due to the presence of surface plasmons and another 243 nm wavelength, SEM showed the presence of particle of different sizes and distributed regularly and small silver nanoparticle. Effect of synergistic silver nanoparticle and antifungal agent (fuconazole) on the biofilm of Candida glabrata, capable of C.gabrata on adhesion of epithelial cells in the absence of silver nanoparticle and fluconazole, no adhesion between epithelial and yeast cells when adding silver nanoparticles, Decrease in surface adhesion between biofilm of the yeast and the epithelial cell when adding fuconazole, When collecting silver nanoparticles with fluconazole and adding it to epithelial cells exposed to C.glabrata, It led to the inability of the yeast to adhere to epithelial cells and then died . All experiments showed the least significant differences at 0.001 level.
Study of Epidemiological Factors According to the Positive Response of Igg of Patients Infected with Blastocytsis hominis in Diyala province, Iraq Muna Hameed Ahmed Alazzawi1, Nabaa Ali Jasim1, Masar H.Ismail3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2821

Abstract

Background: Blastocystis hominis (B.hominis) is the most common intestinal parasite in humans and many other animals. Infections with the organism are spread worldwide and some of them have been asymptomatic, acute symptomatic and chronic. Materials and Method: 100 blood samples were collected from patients with B.hominis which were reviewing to some Hospital and health center in Diyala province during the period from October 2018 to March 2019. To determine the extent of IgG antibody responses in serum patients infected with B.hominis for depending on the chromatic changes resulting from the association of antigens with antibodies. Results: The results of the current study showed the percentage of infection among males was (56.25%) , which is higher than that of females (43.75%), and the age group (3-6) years among males showed the highest rate of infection (33.34%) and the lowest in age groups less from one year and (9-12) years groups at (11.11%). The rate of infection in the age groups(1-3) years and (6-9) years was (22.22%) and in the age group of (1-3) years was the highest incidence among female groups (42.86%), while the age group (3-6) years showed less than that (28.57%) and significant differences at the level of probability less than (0.05). The percentage of B. hominis infection increased among the rural population by (56.5%), male infected, (57.14%) female, while the proportion of males among the urban population was (44.44%) and females reached (42.86%), with a standard deviation of (1.600 ± 0.495) and (1.480 ±0.505) respectively. Conclusions: The presence of immunoglobulin IgG in serum patients has been shown to stimulate the cellular immune response and be indicative of long-term immunity against pathogenic antigens.
Epidemiological and Histopathological Study of Appendicitis in Karbala Province Khetam L.Hussain1, Aiyat hazem al-shugary2, Eman Jabbar Rahim3, Amear Ali Aenad4, Sajida Jawad Mahdi
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2822

Abstract

The current study included an epidemiological prevalence study of appendicitis in Karbala province, where complete data were collected for the year 2018 from Al Hussein medical Hospital. The results showed that the total number of appendicitis in Karbala province was (636 ) cases , divided in to (299) and (286) for male and female respectively, while the secondary removal cases was (69). The percentage of infection was 47.012%, 42.12% and 10.85% for male , female and secondary removal. The statistical analysis showed that there were significant differences under (P> 0.05) between percentage of infection in male and female (0.01). Also this study included histopathological examination of appendectomy specimen. After surgical removal of appendix, the specimen directly fixed with 10% formalin and then do tissue processing. The microscopic examination showed obstruction of the lumen and infiltration of inflammatory cell within lumina properia, muscularis and adventatia.the mucosal epithelial was destruction with ulceration, also there are extensive lymphoid hyperplasia.
Molecular investigation of virulence factors genes in streptococcus pyogenes by PCR Russell Issam AL-Daher1, Maysa S. AL-Shukri2, Hayam K . AL-Masoodi3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2823

Abstract

This study was designed to detect some genes associated with important virulence factors in streptococcus pyogenes . A total of 200 throat swabs were collected from patients suffering from pharyngitis from both sex and age from (1 - 15) years. carried out from ENT unit in Al-Hilla General Teaching Hospital and Al Noor Hospital during the period from January 2018 to December 2018 , Out of the 200 samples only 177 samples showed positive bacterial culture ,No growth was seen in other 23 samples ,The results indicate that the rate of Streptococcus pyogenes isolated from patient with pharyngitis is 30 ( 15%), others bacterial growth 147 ( 73%), and no growth 23 (12%). Molecular detection of virulence factor genes was done like M protein (emm) gene , the result shows that these genes were detected in all isolates bacteria (100%) with molecular length (914 bp) , SpeA gene was carried by using specific primer and it was found that (3.3%) isolates give positive result for this gene with amplicon (576 bp) , SpeB gene the result shows that (100%) isolates contain the gene with molecular length (952 bp) , SpeC gene also study by using specific primer at molecular length (405 bp) and the result s show that (75%) give positive results to this gene , the mac gene in all S.pyogenes isolates the results show that (30%) give positive , results to this gene with molecular length (389 bp) , scpA gene in all isolates the result shows that 17 isolates (56.6%) give positive result to this gene with amplicon size (622 bp)
Iron Deficiency among Patients with Febrile Seizures in Al Ramadi Maternity and Children teaching Hospital, Western Iraq Sahar j. AL- Hiali
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2824

Abstract

Background : Febrile seizures are the most predominant neurological disorders in children between 6 months-5 years, at the same time iron deficiency anemia is one of nutritional insult that implicated as risk factor for occurrence of febrile seizures. Objectives: to estimate the role of iron deficiency as a risk factor for febrile seizures in children aged 6 months -5 years, western Iraq. Patients and method:- A case control study has been conducted in AL Ramadi maternity and children teaching hospital during January to October of 2016. All children with history of febrile seizures aged 6 months-5years were involved as cases group, others with febrile illness and no seizures were considered as control group. Data from 58 child of each group were collected. The data include age, sex ,temperature, causes of febrile illness, Hb ,hematocrit and s. ferritin levels. The data were subjected to statistical analysis run under IBM SPSS ver. 23. Results:- Means age ± SD were 27.48±14.83 & 23.24±14.37 months in cases and control groups respectively with no significant difference between them. Most of the children (65.52%) of those with febrile seizures were less than 3 years old. Mean temperature of cases group (38.860 C) was found to be significantly greater than that of the control group (38.520 C) at p-value <0.01. Mean Hb level ,PCV%, s. ferritin level were significantly lower in cases group than in control group. Proportion of iron deficiency anemia was significantly higher in febrile seizure group (65.52%) than in control group (31.03%) at p-value< 0.01. Conclusions: Occurrence of IDA in cases group is found to be more than 2 times of that in the control group. Early detection and proper treatment of IDA can play a prominent role in limitation the prevalence of febrile seizures among children below 5 years.
Antibiotic Susceptibility of Bacteria Isolated From Under Nails Zahraa AL-Taee1, Sura Ihsan Abed Jabuk1, Raflaa S.H. Hussian1, Zainab H. Kareem2, Yasir H. AL-Mawlah
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2825

Abstract

This study was designed for isolation and diagnosis of bacteria from under long dinger nails of a wide population of student (kindergarten, primary schools, elementary schools and university students) and study the antibiotic sensitivity for isolated bacteria. From 100 sample the bacterial isolate were Staphylococcus sp.(56 isolate), Bacillus sp.(1 isolate), Streptococcus sp. (1 isolate), Escherichia coli (40 isolate), Salmonella (2 isolate), Enterobacter(10 isolate), Klebsiella (10 isolate), Serratia (5 isolate) and Pseudomonad (6 isolate). The most effected antibiotic on all types of bacteria isolated from under the long nail was Gatifloxacin and the lowest effect was Cefazolin antibiotic. The concept of the study was isolating and determining bacteria, found under the long fingernails and studying their antibiotic sensitivity.
Removal of Tetracycline from Aqueous Solutions using Pomegranate Peels Residues Accessing to ZRL Rajaa S. Abass1 Suad Turky Ali1, Tamara Sami Naji2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2826

Abstract

Antibiotics are harmful pharmaceuticals to ecosystems in general and aquatic systems in particular. Therefore, its remediation from water bodies is a topic of great importance for water treatment and purification workers. This research investigates the removal of one of the most famous types of antibiotics, which was tetracycline from simulated synthetic aqueous solutions by adsorption technique using non-toxic, low cost and available agricultural waste which was pomegranate peels. The adsorption experiments were performed in adsorption laboratory unit of batch mode at different operating conditions and laboratory temperature. The operating parameters studied included pH of solution, dose of adsorbent media, treatment time, agitation speed and initial concentration of tetracycline. The results showed the ability of pomegranate peels to extract tetracycline from aqueous solutions with high efficiency of 81.55%. The results also showed that the percentage of antibiotic adsorption from aqueous solutions was inversely correlated with increasing the initial concentration and acidic function of the tetracycline solution while it was directly proportional to the amount of pomegranate peels, agitation speed and treatment time. In this style, one of the most important types of antibiotics that contaminated water was disposed of by a cheap material and using a simple, economical and environmentally friendly method accessing to the principle of zero residue level (ZRL).
Complications of Laparoscopic Cholecystectomy in a Sample of Patients Admitted to Al-Ramadi Teaching Hospital, Anbar-Iraq Tarek Mahdi Salih
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2827

Abstract

Laparoscopic cholecystectomy is currently thought as a surgical procedure that can be done with less risk of complications. However, intraoperative and postoperative complications were existing in 56 (15.01%) patients. Open surgery is the case when laparoscopic cholecystectomy fails to be proceeded with due to many causes such as difficult Calot triangle dissection and empyema of gallbladder. The high occurrence of cholecystitis in the group of patients aged 40-60 (56%) with 151 female and 59 males initiating the floor to many arguments that may help give a good understanding for such a problem. Iatrogenic perforation of gallbladder was the most common cause of intraoperative complications. Wound infection was the most cause of postoperative complications. Difficult Calot triangle dissection was the most common cause to convert to open surgery.
Physiological Study Comprising the Sequelae of Magnetic Radiation on Human Wissam Sajid Hashim1, Azal Hamoody Jumaa2, Noor Thamer Alsaadi 1, Ayad Gaber Arean1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2828

Abstract

The sequelae of magnetic radiation of the towers of cellular phones were evaluated in this study depending upon a random social volunteer human male samples of ages 25 – 50 years and they were dwelling in houses close to the towers of cellular phones of distances not more than 150 miters far. The samples of these people were labelled as “Subjected”. Seventy five samples of blood were gained on a base of ages as twenty five from ages 25 – 30, twenty five from ages 35 – 40, and twenty five from ages 45 – 50 in addition to seventy five samples of people who did not dwell close to the towers and of the same previous pattern of ages and their samples were labelled as “Non subjected”. The results have demonstrated a significant elevation in transaminases (AST , ALT), blood calcium (Ca++), blood potassium (K+), total serum cholesterol (TSCH), triacylglycerols (TAGs), very low density lipoprotein (VLDL), and low density lipoprotein (LDL) .

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