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INDONESIA
Indian Journal of Forensic Medicine & Toxicology
Published by Official Organ of Indian Association of Medico-Legal Expert
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Public Health
Arjuna Subject : Kedokteran - Epidemilogi
Articles 5,497 Documents
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Production, Analysis and Optimization of Inulin Produced from Pseudomonas fluorescens Alyaa Razooqi Hussein1, Alaa Raheem Kadhim2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2849

Abstract

Inulin is a natural polysaccharide produced from organisms and microorganism. Inulin is a type of fructan that ?-amylase cannot digest it and cannot hydrolysis by hydrolytic enzymes. It was used to get non-fat fermented milk and it’s a type of prebiotics that induce growth and activity of probiotics bacteria, thus improving the health. Pseudomonas fluorescens have the capacity to produce inulin when grown in media supplied with sucrose. Twenty-two of bacterial isolates were belong to Pseudomonas fluorescens depending on structural features, microscopic checking, biochemical analysis and flourescent pigments that produced on King B medium. Ten isolates of P. fluorescens had strongly degree of mucous growth on solid production medium, the gummy and mucous manifestation on agar medium containing sucrose were give rise to inulin production. Inulin produced from P. fluorescens was analyzed by Fourier Transform Infrared Spectroscopy to detect functional groups which it was (C-O, CH, OH and C=O) and by Thin Layer Chromatography to determine its components of monosaccharide. Inulin distinguished as dark spot on white background and the Rf of it was (0.58). The best product of inulin were in production salt agar medium containing 20% sucrose, pH =7, Temperature =370C without nitrogen sources and the inoculum size 1%, it was (3.2 gm ?100 ml). The effects of bacterial inulin on the growth of Saccharomyces cerevisiae was studied by culturing it in medium supplemented with 3% of bacterial inulin at 30°C for 48 hrs. The results showed there was no remarkable effect of inulin on Saccharomyces cerevisiae growth in comparison to control.
Epidemiology of Hepatitis B and C in Al-Muthanna Province Adian Abd Alrazak Dakl1, Wafaa Ayad Alnuaimy1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2851

Abstract

This study systematically reviewed and synthesized available records of hepatitis B and C prevalence in Al-Muthanna province through the last five years from early 2014 till the end of 2018 in Women and Children Teaching Hospital at Al-Muthanna province. The study recorded high prevalence of hepatitis virus at this region, most of the patient were females and HCV was the most prevalent between them, the year of 2016 recorded the highest infection rate. Conclusion: High rate of Hepatitis C virus infection among of thalassemia patients
The Impact of Congenital Heart Diseases on Growth Parameters in Children and Their Correlations with Leptin Levels Fouad Shareef1, Amal Salih Mahdi Bayee2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2852

Abstract

Children with congenital heart disease (CHD) prone to stunted growth by different parameters including weight, height and head circumference compared to a control groups, to accomplish such aim (110) children aged ranged (20 days – 13 years with CHD) have enrolled in this study, (83) patients with a cyanotic CHD (75.45%) and (27) cyanotic type (24.54%) were compared with (171) healthy controls of similar age and sex groups. This study was done in Al-Hilla teaching hospital during the period November 1, 2017 to October 11, 2018. The results indicate a significant (2.06) retardation in all parameters in patients with CHD related to control and there is a significant retardation in weight (z = 3.06) in children with cyanotic CHD related to a cyanotic type. Also there is a significant retardation (z = 1.01) in height gain in children with cyanotic related to a cyanotic type and finally there is a significant (z = 2.09) decrease in head circumference in children with cyanotic CHD related to a cyanotic type. Serum leptin levels were also lowered in all patients with CHD. The results concluded that children with (CHD) experience early and continues decrement of all growth parameter during their life.
Hormonal and Mineral Imbalance Effect on Bone Resorption in Predialysis Iraqi Patients with Chronic Kidney Disease Inaam Ahmed Ameen1, Sarah Hashim Mhaibes2, Shahad Wisam Ahmed3, Kaled Nather Taha4 And Dhikra Abdul
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2853

Abstract

Introduction: Chronic kidney disease mineral bone disorder is a metabolic bone disease present in almost all uremic patients. The aim of this research to indicate the stage of chronic kidney disease (CKD) that affect the bone metabolism that leading to the mineral and hormonal imbalance by studying the relationship among osteocalcin , glomerular filtration rate (GFR), parathyroid hormone, calcium and phosphorus levels in the blood. Method: The study included 52 patients with predialysis chronic kidney disease stage 3-5 and 40 apparently healthy relatives accompanying the patients. Glomerular filtration rate (GFR) was calculated for each patient. Renal function tests, including serum levels of urea, creatinine, a biochemical marker of bone metabolism: osteocalcin (OSN), calcium, phosphorus, and parathyroid hormone (PTH), were measured for each participant. Results: Serum urea and creatinine levels were significantly higher in CKD patients Than that of apparently healthy control. There is significantly higher serum parathyroid hormone, serum phosphorus, serum osteocalcin (P<0.01, P<0.01, P<0.01 respectively) in CKD patients than that of the healthy control group. While low serum calcium level in CKD patients as compared to the corresponding group (P<0.01). Conclusion: Hyperphosphatemia and hypocalcemia in the end stage of predialysis CKD patients lead to increase parathyroid hormone secretion, which causes high bone turnover characterized by significantly high serum osteocalcin in these patients. Parathyroid hormone and osteocalcin were used as a biomarker for the development of bone and mineral disorders in predialysis CKD patients.
Robust Controller Electromyogram Prosthetic Hand with Artificial Neural Network Control and Position Saygin Ahmed1, Aydin S. Ahmed2, Bülent Yilmaz3, Nuran Dogru1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2854

Abstract

In this study, we proposed and designed a new control method for an electromyographically (EMG) controlled prosthetic hand. The objective is to increase the control efficiency of the human–machine interface and afford greater control of the prosthetic hand. The process works as follows: EMG biomedical signals acquired from Myoware sensors positioned on the relevant muscles are sent to the robot that consist of hand, Arduino and MATLAB program, which computes and controls the hand position in free space along with hand grasping operations. The Myoware device acquires muscle signals and sends them to the Arduino. The Arduino analyzes the received signals, based on which it controls the motor movement. In this design, the muscle signals are read and saved in a MATLAB system file. After program processing on the industrial hand which is applied by MATLAB simulation, the corresponding movement is transferred to the hand, enabling movements, such as, hand opening and closing according to the signal stored in the MATLAB system. In this study, hand and fingerprints were designed using a three-dimensional printer by separate recording finger and thumb signals. The muscle signals were then analyzed in order to obtain peak signal points and convert them into data. These results indicate the effectiveness of the proposed method and demonstrate the superiority of the method for amputees because of the improved controllability and perceptibility afforded by the design.
Study the Genotoxicity of Aqueous and Alcoholic Extracts of Adhatoda Vasica on the Roots of Allium cepa L. by RAPD-PCR Technique Adnan Owayez Jasim1, Mohammed Mahdi Jawad1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2867

Abstract

The Molecular technique RAPD-PCR used to detect the genotoxicity of different concentrations of aqueous and alcoholic extracts of Adhatoda vasica leaves on onion Allium cepa L. roots. Five concentrations (1%, 2%, 3%, 5%, and 7.5%) and (10%, 15%, 20%, 30% and 40%) were adopted for alcoholic and aqueous extracts respectively. Ten arbitrary primers used in this study, only eight showed polymorphic bands in the gel and two primers were neglected because they did not show any polymorphic bands for all samples. The aim of this study is to detect the toxic effect of the extracts on the root of onion. The emergence and disappearance of bundles in the genome of onion plant Allium cepa L. Treatment with extracts was studied. The genetic relationship tree was established and the genetic distance calculated based on the results obtained from the gel electrophoresis to clarify the toxic effects of the extracts .The results showed that the concentrations that gave the highest effect and the most toxic for aqueous extract is 40% and for the alcoholic extract is 7.5% which are recommended as effective concentrates if used as a pesticide.
Histological Study of the Effect of Isoxicam on Ovary of Albino Mice Mus Musculus Ali Khudheyer Obayes1, Wurood Mohamed Mutar2, Rasha Hamid Ayub3, Sahar Abdullah Mohammed4
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2868

Abstract

Non-Steroidal Anti Inflammatory Drugs (NSAIDs) are the most prescription as therapeutic drugs, used to treat of rheumatic diseases, due to analgesic, antipyretic and anti-inflammatory activity. Isoxicam is a member of NSAIDs group use to stop inflammation, pain associated with arthritis, osteoarthritis, ankylosing and spondylitis. The goal of the present study is to revealed the effect of different doses of Isoxicam on ovaries tissue in mice. Twenty four female mice are randomly divided into control (n = 6) and experimental (n=18) groups. The experimental groups are subdivides into three groups . Each administrated by (0.0714, 0.1428, 0.71428)mg/kg/day for twenty days, respectively; however the control group just injected by distill water. In twenty day, mice were killed and ovaries tissue was prepared for light microscopic examination. All the experimental animals were injected by drug revealed a hyperplasia of germinal cells on the surface of ovary, tongue like projection of primordial oocytes extend to the medulla, multiple oocytes with disarrangement of follicles and deficient of follicular fluid associated with disappearance of oocytes, vacuolation in the cortical layer of the ovary, compressed premature follicle, hypercellularity of follicular cells, degeneration of germinal layer of cortex surface and hyperplasia of primordial oocytes, therefore it is recommended that using of this drug have many side adverse on female fertility.
Genotyping of Tumor Necrosis Factor-? in Inflammatory Bowel Iraqi Patients Inas Fadhe1, Wafaa Sabri Mahood2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2869

Abstract

The TNF-a gene considered as strong candidate for immune modulator and pro inflammatory cytokine responsible for genetic susceptibility of chronic disease such as the initiation and development of inflammatory bowel disease (IBD).The aim of this study is to investigate the genetic polymorphisms of -1031 in TNF-a gene with susceptibility of Iraqi IBD patients Method: The total number of this study 95 blood samples (75 Iraqi IBD patients and 20 from healthy individuals as a control group). The genetic polymorphisms in TNF-a-1031 gene was investigated using restriction fragment length polymorphism (RFLP) and Sanger sequences techniques. Results: The genotype allele frequency of -1031 polymorphism was significantly higher in CD Iraqi patients (P=0.042 , Chi 4.612. OR= 0.72-1.64) ,C allele may be have protective role , whereas the T allele may increase susceptibility to IBD. In conclusion: The TNF-a -1031 gene polymorphisms in promoter region have an important role in the occurrence of inflammatory bowel disease of Iraqi population especially in CD patients although some results didn’t give us a significant differences.It’s possible that C allele may be have protective role , whereas the T allele may increase susceptibility to IBD
Antibacterial activity of Eruca Sativa Seeds Aqueous Extract Against Human Pathogenic Bacteria Madeha H. Hussain4, Aml Hendi Salih1, Rajaa Hendi Salih2, Ali S. Hassoon3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2871

Abstract

This research was achieved with the aim of detecting the Antibacterial efficacy of the watery extract Eruca of Sativa seeds toward human pathogenic bacteria, four Pathogenic species of bacteria(Enterococcus faecalis, staphylococcus aureus, pseudomonas aerogenoso, and Salmonella typhi) . were isolated from different clinical samples at al. Kademia teaching hospital Laboratory. Results: The results showed The extract inhibits gram positive bacteria and the mean inhibitory zone for strepto coccus faecalis and S. aureus was 10.4 mm and 14.0 mm respectively. Conclusion: The extract had no effect on gram negative bacteria.
Possible Role of Toxoplasmosis on Gene Sequence Alteration in Patients with Cardiovascular Diseases Nazar Sh. Mohammed1, Ammer Abd. Mohammed2, Salwa S. Muhsin1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.2881

Abstract

Toxoplasmosis is one of the risky infection my lead to cardiovascular diseases. One hundred therein patients samples were collected with Heart Diseases and there were infected with Toxoplasmosis, in Baghdad educational Hospital from period 1st January 2019 to 1st September 2019. The results show that prevalence of Toxoplasma gondii among Hypertensive disease and Myocardiopathy with Heart Diseases. The genotype of MYLK3 exon to Human cardiac gene by gel electrophoresis, Lane M markers correspond to 500 bp ladder lane 2 of gene band with 600bp. Mutation occurrence in exon 8 the gene sequence CCCAGCCGG were change to CCCATCCGG, and in exon 9 the change occurrence in sequence CTCAAGCCGGAG to CTCAAGGTACAA

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