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Indian Journal of Forensic Medicine & Toxicology
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Core Subject : Health,
Articles 5,497 Documents
Impact of Pregnant Adolescents’ Knowledge about Preventive Health Behaviors during Pregnancy upon Pregnancy Outcomes in AL- Diwaniyah City Fadia H. Ali; Hala S. Abdul Wahid; Ekhlas A. Hussein
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17801

Abstract

Objective(s): The aim of the study is to evaluate the impact of pregnant adolescents’ knowledge aboutpreventive health behaviors during pregnancy upon their pregnancy outcomes .Methodology: A quasi-experimental design, using the evaluation approach for the determinationthe impact of pregnant adolescents’ knowledge about preventive behaviors during pregnancy uponpregnancy outcomes in Al-Diwaniyah city from the period 17th January 2020 to 1st June 2021.Non- probability, purposive sample of (35) adolescent pregnant are selected from those who visitAl-Diwanyiah Maternity and Pediatric Teaching Hospital. Data are collected through the use of thestudy instruments (questionnaire) in a form of Google format and through video calls as means of datacollection. Data were analyzed through the use of descriptive statistical data analysis approach andinferential statistical data analysis approach.Results: Results of this study indicate that most of the pregnant are between (16-19) year old (57.1%),(34.3%) are primary school graduates, (71.4%) are housewives, (77.1%) are living in rural area and(80%) are earning an income of (300-600) thousands ID. The overall evaluation of pregnant adolescents’have a lack of knowledge about preventive health behaviors during pregnancy without significantrelationship between pregnant adolescents’ knowledge and their demographic characteristics. As wellas, pregnant adolescents’ knowledge about preventive behaviors during pregnancy does not impose anyeffect upon their pregnancy outcome.Conclusions: The study’s unique finding is that pregnant adolescents have a lack of knowledge aboutpreventive health behaviors during pregnancy, and this knowledge does not have any effect on theirpregnancy outcome.Recommendations: Improving pregnant adolescents’ health literacy is the responsibility of healthcaresystems and healthcare professionals through emphasis by the Ministry of Health role through theantenatal care units to take a part and dissemination of education about the preventive health behaviorsamong pregnant adolescents especially primigravida, and particularly with each specific trimester.
Molecular detection of Brucella canis in Blood of Dogs Hussain Fawzi Saud; Taha Yassin Ghani
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17802

Abstract

The aims of this study was to evaluate a PCR for detecting Brucella canis in the blood of dogs, using aprimer pair designed for Brucella spp.A study was conducted on 150 blood sample collected from dogs suspected to Veterinary Hospitalin Baghdad / Aden Square. All blood samples (150) were tested by PCR technique using a commonprimer of the 23S ribosomal RNA (23s RNA) gene and specific primer for brucella canis (B0548).The genomic DNA was extracted and PCR was applied. Our study recorded 5.3% of brucellosis incommon primer and 3.3% in specific primer for brucella canis in dog in Baghdad city, the sequencesof Brucella canis in dog in different isolates in our study recorded 99% compatibility recording toNational Center Biotechnology Information (NCBI). Following correspondence from National Centerfor Biotechnology Information, the 23S ribosomal RNA gene was registered, given an agreementnumber, and became a resource for Iraq and Middle East, as well as the rest of the world. As more typestrains are published, this set will grow, and it can be download from NCBI at: https://www.ncbi.nlm.nih.gov/nuccore/.From this study we can conclude that, the percentage of Brucellosis in dogs in Baghdad city is 5.3% and3.3% in a common and specific primer, respectively and the molecular method (PCR), is a good ideafor confirmation of diagnosis of Brucella canis infection in dog.
Extraction of Outer Membrane Proteins of Proteus Mirabilis Isolated From Urinary Tract Infections and their Immunological Effect In Vitro Heba safaa Abdelqader; Ibrahim A. A. Rahmman
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17803

Abstract

300 urine samples were collected from patients visiting Fallujah city hospitals (consulting clinicsof Fallujah Teaching Hospital and Women and Children Hospital) for the period from 1/9/2020 to30/12/2020, of different ages and for both sexes. The results showed that out of 300 urine samples were244(81.33%) A sample with a positive result for bacterial culture, and it was found there were highsignificant differences between the positive and negative cultures. Females and males (64.70) (35.29),respectively.Isolates of Proteus mirabilis were diagnosed by observing the cultivar (colonies) and microscopic(bacterial cells) characteristics, biochemical tests and diagnostics with Vitek device. Antibiotic sensitivitywere done at 12 types, Antibiotic resistance wre vary from Cefotaxime, Rifampicin, Ceftriaxon (100%),Cefixime, Ciprofloxacin (76.4%), Levofloxacin (64.7%), Nitrofurantoin (58.8%), Gentamicin (52.9%),Amikacin, Nalidixic acid (35.2%) (17.6%) Imipenem (11.7%).Extraction and partial purification of outer membrane proteins (OMPs) from the most antibioticresistantisolate using lysozyme, DNase, RNase, and N-Lauroyl-Sarcosinate enzymes. associated withthe ion exchanger.To study the effect of the antigen of outer membrane proteins, some immunoassays were performedin vitro and the following results appeared: The use of concentrations (100, 50, 25) of OMPs antigenled to low significant of lymphocyte viability percentage (96.66, 97.66,98.0), respectively compared toWith the negative control (99.66), the concentrations (100,50)% significantly reduced the percentageof survival of PMNs cells (97.66,98.33), respectively, compared with the control (99.66), while theconcentration of 25 did not affect the survival of PMNs cells (99.66). The above concentrations showeda low significant in the diameter of the migration circuit of PMNs cells (8.830,10.59,12.21) comparedwith the negative control (15.21), while these concentrations led to a high significant in the percentageof formazone-forming PMNs cells (64.66,58.0,47.0) compared with the control (36.66). ) and a highsignificant in the sensitivity of lymphocytes (4.0,3.39,1.87), as the absorption spectrum values forconcentrations (100,50,25) reached (0.92,0.74,0.41), respectively, compared with the negative control(0.22) and the positive control (1.53). The concentrations of the used proteins increased the phagocyticindex (PI) of PMN cells ,The phagocytosis coefficient was high significantly with time until it reached itsmaximum at 90 minutes, and from the statistical analysis it was found that the phagocytosis coefficientincreases with increasing concentration of proteins.
Impact of Psychosocial Domestic Violence upon Reproductive Health during Corona Virus Pandemic among Women Attending Primary Health Care Centers in Baghdad City Zahraa Burhan Aldeen; Iqbal Majeed Abbas
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17804

Abstract

Objectives: To assess Psychosocial domestic violence among women and assess the impact ofPsychosocial domestic violence on women’s reproductive health during the COVID-19 pandemicMethodology: A descriptive analytical study was conducted on women subjected to psychosocialdomestic violence and impacts of psychosocial domestic violence on women during the Coronapandemic period. A deliberate sample of (150) women exposed to violence was selected by a memberof her family.Results: The result of the study showed that the highest percentage (79.4%) of the study samplemembers ranged in age from (20 to 34) years, most of whom were housewives, three quarters of thestudy sample had a low educational level. Psychosocial domestic violence was at a higher level ofrelative sufficiency.Conclusions: The results indicated that all study sample suffer psychosocial domestic violence duringchildbearing age, but most of the study sample considered psychosocial violence to be the mostinfluential on their lives than other effects.Recommendations: The study recommends that women be screened for psychosocial domesticviolence during childbearing age. psychosocial domestic violence topics into education curricula,using social media, the availability of health services, and supporting the strengthening of cooperationbetween social agencies, justice and the police through law enforcement and research to promote andprotect women’s rights.
Thiopurine S-Methyltransferase Genotyping in Iraqi Childhood Acute Lymphoblastic Leukemia Patients ; A Single Institute Study Esraa Ali Kadhum; Manal K Rasheed; Hasanein H.Ghali
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17805

Abstract

Background: Acute lymphoblastic leukemia (ALL) is the most common cancer seen in childrenworldwide and is the most common cancer in children under 14 years of age. Although there havebeen major advances in treatment approaches for childhood ALL, serious toxicities such as profoundleukopenia frequently affect treatment and lead to life threatening consequences such as severeinfections and even death. There has been a lot of interest in inter-individual differences in drugmetabolizing enzymes in order to better adjust drug dosage and therapy. In this regards, ThiopurineS-Methyltransferase (TMPT) was the first pharmacogene that showed a substantial associationwith Mercaptopurine (6-MP) maximum tolerated dose and 6-MP related toxicities leading to theimplementation of TPMT genotyping before drug administration Aim: To identify the most commonTPMT polymorphism (TPMT*3A, TPMT*3B and TPMT*3C) and its frequencies in a sample of IraqiALL paediatric patients. Methods: A cross sectional study was performed for 79 patients with Acutelymphoblastic leukemia . Genotyping for (*3A , *3B , *3C ) the TPMT gene was performed by theallele-specific multiplex-PCR analysis method. Results: The TPMT*3A mutant allele was found in 18patients with allele frequency of (22.8 %), while TPMT*3C mutant allele was detected in 5 childrenwith allele frequency of 6.3% . But TPMT*3B mutant allele was not detected in whole sample ALLpatients .The correlation between gender and the polymorphism was not statically significant as p-value0.23.. Conclusion: TMPT genotyping is an essential tool to reduce the cytotoxic effects of the anticancerdrug 6-MP in Iraqi paediatric patients with ALL .
The Association of Low Taurine Levels with Diabetic Neuropathy in Iraqi Patients Rand Thair Abdulkader; Nada A. Kadhim; Firas Younus Muhsin
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17806

Abstract

Aim of this Study: this study has been carried out to evaluate the role of taurine, and lipid profile inprogression of diabetic complication.Methods: this work included 81 Iraqi people (age ranged 40-65). Healthy control subjects (G1group=28), type 2 diabetes mellitus without any complications (G2 group=26), and type 2 diabetesmellitus with peripheral neuropathy (DPN) (G3 group=27). Diagnosis of the disease was based onseveral symptoms and questions under the supervision of the consultant supervisor at the center. Serumtaurine, lipid profile (Cholesterol, triglycerides, HDL, LDL, and VLDL) besides fasting blood glucoseand plasma HbA1c were determined for each participant. Results: The results of taurine revealed thatthere was a highly significant decrease in G2 and G3, as compared to G1. The mean values of serumcholesterol showed no significant difference between G2 and G3 as compared with G1while serumtriglycerides (TG), LDL and VLDL showed a high significant increase in G2 and G3 as comparedto G1. The results of serum HDL levels showed that there was a high significant decrease in G2 andG3 as compared to G1. No significant differences were found in body mass indices in all the studiedgroups while WC, HC, WHR, and WHtR showed high significant increase in G2and G3 as comparedto G1. Systolic blood pressure significantly increased in G2 and G3 while diastolic blood pressuresignificantly increased in G3 only.Conclusion: Serum taurine levels were decreased in patients with T2DM and DPN .The reducing intaurine synthesis was not related to FBG, HbA1c, and dyslipidemia. We also found that taurine levelsmay be more important in the development of diabetes complications.
Immunohistochemistry Detection Apoptosis Related with ORF Virus Infection in Sheep Based on Caspase 3 Detection from Selected Farms in Basrah Yasmeen Jasim Mohammed; Eman Hashim Yousif
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17807

Abstract

This study was designed for the molecular detect of apoptosis related with ORFV infection in sheepbased on detected caspase (3) by immunohistochemistry. The samples were taken from the skin of thelips of animals infected with contiguous ecthema. The ability of the virus to induce apoptosis was alsoverified using cellular immunohistochemistry and the use of a polyclonal anti-caspase (3) antibody todetect apoptotic activity. The results of the study revealed positive expression of caspase (3), where ahigh percentage of caspase (3) was observed in the affected cells in the epidermis of the lip, in additionto the presence of overexpression in all layers of the epidermis, with the presence of small areas ofapoptotic cells compared with the control group.
Pregnancy Rate in Synchronized Iraqi Awassi Ewes Inseminated Artificially and Naturally Abas, H. Kassem; Alyasiri E.A; Al-Hamedawi; T.M., Hmod M. Ajeel
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17808

Abstract

This study was carried out to evaluate the efficacy of imported Australian frozen semen straws inachieving successful artificial insemination program in estrus synchronized ewes. This study conductedon twenty-one Awassi ewes, aged 2 - 4 years, and four fertile rams (two rams for natural mating and twofor detecting of estrous by using apron) in Baghdad province (Rumintia Research station, departmentof animal resource research, state Board for Agriculture Research, Ministry of Agriculture/ AbuGhraib district), for the period from November 2020 to March 2021. Pregnancy diagnosis was doneby ultrasound to detect reproductive status of the ewes, then all non-pregnant ewes were synchronizedby using vaginal sponges (60 mg of Medroxy acetate Progesterone / MAP) for 10days plus 500IU ofPregnant Mare Serum Gonadotropin (PMSG) in day 10, all animals were divided randomly into threeequal groups each of seven ewes according to the type of insemination, G1 (1straw) and G2 (2 straws/8 hrs. interval) and G3 serviced naturally by fertile rams then the females were isolated from the males.The response of animals to hormonal treatment was 21, with a response rate of 100% in all groups withno significant difference with mean durations of response recorded 63.6± 11.7, 66.7± 12.2, and 62.8±10.7 for the three groups, respectively. The pregnancy rate with single, double AI and natural matingwas 41.86 and 71.41 and 71.42 percent, respectively with a highly significant difference (P<0.01)between G1 and G2. In conclusion, under field conditions, double AI with 20 ×106 sperm proved to besuitable for generating high pregnancy rates in Iraqi Awassi ewes.
Molecular Study of Eimeria species in Quail birds (Coturnix coturnix japonica) in Thi-Qar Province, Southern Iraq Muntadher. M. Flayyih AL-Zarkoushi; Mohammed. Th. Salih AL-Zubaidi
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17809

Abstract

Fecal samples were collected from 330 quail birds from different areas of Thi-Qar province, Iraq. Allsamples were examined by traditional laboratory methods. Four species of Eimeria were diagnosed inquail (Eimeria bateri, Eimeria uzura, Eimeria tsunodai and Eimeria fluminensis), with a total infectionwas 64.54% (213 out of 330). DNA was extracted from 100 positive samples confirm the small subunitrRNA (18s) gene by Polymerase chain reaction using a general primer for Eimeria species, Sevengenotypes were according to the identify of DNA sequences of Eimeria species in Japanese quail, theaccession codes to NCBI-Genbank. using A comprehensive phylogenetic tree was generated in thepresent study, this details were being analyzed. This was the first molecular study in Iraq for Eimeriain common quail.
Study the Factors affecting the Production of Coagulase Enzyme from Clinical Bacteria Isolated Najemaddin Abdullah Hamad; Dhafer Alrawi
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17810

Abstract

This study included isolation and diagnosis of Coagulase Positive Staphylococci bacteria fromclinical samples of (21) samples from the Teaching Hospital for Women and Children in Ramadi andincluded nasal swabs, Ear swabs, Normel skin swabs, Wound swabs, Urine Swabs and Abscesses.These isolates were diagnosed according to phenotypic and biochemical tests and vatic. After diagnosingthe developing colonies, it was found the Gram-positive bacteria formed a percentage (71.42%) of theclinical samples out of the total isolates that gave growth on the culture media, and the Staphylococcipositive for the plasma coagulase enzyme (Coagulase Positive Staphylococci) was found. (71.42%) ofthe total Staphylococci bacteria obtained during isolation and diagnosis of clinical samples and a typeof Staphylococcus positive for coagulase (CPS), Staphylococcus. aureus, was diagnosed based on theSlide and Tube Coagulase Test.The sensitivity of CPS-positive staphylococcus to the antibiotic against the bacteria was tested and itwas found that the most effective antibiotic against S. aureus bacteria was Azithromycin as well as theantibiotic Gentamycin, as indicated by S. aureus has demonstrated resistance to Erythromycin as wellas Ciprofloxacin.The different conditions for the production of the plasma coagulase enzyme (Coagulase) for the bacterialisolate of local number (5) were studied, and it was noticed that there is a great similarity in theseconditions except for some differences. The study and it was noticed that the appropriate temperature inthe production of the enzyme for the local bacterial isolate number (5) is the temperature (35) C, and itwas noticed that the pH that led to the increase in the production of the enzyme is (PH10).After (24hr)incubator , and the results were consistent in terms of good production of the enzyme in the mentionedisolation when using a incubator shaker at a speed (100) .