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<![CDATA[Cytotoxic Potential of Purified Arginine Deiminase from Streptococcus spp ]]>
<![CDATA[Rusul Madhat Abdullah]]>;
<![CDATA[Ruqaya Mohammed Al-Ezzy]]>;
<![CDATA[Asmaa Ali Hussain]]>
<![CDATA[ Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology ]]>
Publisher : <![CDATA[Institute of Medico-legal Publications Pvt Ltd ]]>
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DOI: 10.37506/ijfmt.v15i3.15458
<![CDATA[The results that presented in the study were based on collecting one hundred and ten clinical samples of oralcavity, burns and wounds. Primary and secondary diagnositic showed that 14 out of these isolates belongedto Streptococcus spp.. The ability of these isolates in arginine deiminase enzyme production was screenedand the results showed that these isolates were arginine deiminase producers with variable degrees. It hasbeen found that one of these isolates which were Streptococcus mitis S5 had the highest specific activity(0.184 U/mg protein), therefore it was chosen for further study. Results of the optimum conditions forarginine deaminase production reveal that the maximum arginine deaminase production was achieved aftersupplementation of the production medium (pH 7.5) with 1% maltose as carbon source, 1.2% tryptone asnitrogen source, 30mM as arginine concentration and incubated at 37ºC for 18h. Under these conditions,ADI purified in three steps including; precipitation with 70% saturated ammonium sulphate, dialysis then ionexchange chromatography using DEAE- cellulose column and the last step was gel filtration chromatographythroughout Sephadex G150 column. Specific activity of the purified enzyme was increased up to 18 U/mgwith 5 folds of purification and 70.8 % enzyme recovery.]]>
<![CDATA[Involvement of Insulin Like Growth Factor-I Gene Polymorphism with Diabetes and Diabetic Nephropathy in Individuals with Type 2 Diabetes in Babylon Province ]]>
<![CDATA[Maytham Ahmed Abdul Aemah]]>;
<![CDATA[Moaed Emran Al-Gazally]]>;
<![CDATA[Ali Jasim Al-Sultani]]>
<![CDATA[ Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology ]]>
Publisher : <![CDATA[Institute of Medico-legal Publications Pvt Ltd ]]>
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DOI: 10.37506/ijfmt.v15i3.15459
<![CDATA[Concerning type 2 diabetes mellitus (T2DM), diabetic nephropathy (DN) is a chiefmicrovascular complication.Preceding studies have proposed that insulin-like growth factor-1(IGF-1) signaling might do a significantrole in DN. The existing study was implemented to explorethe allianceof single nucleotide polymorphism(SNP) rs6214 of the IGF-1gene with T2DM and DN. The study consists of three groups, the first oneincludes50 subjects, those were apparently healthy were considered as controls. The second oneinvolves 69 patientswith T2DM without nephropathy and the third oneencompasses 89 patients who suffer from T2DM withDN. The analysis of results elucidated that thegenotype CT was decided to significantly increase the hazardof T2DM by about 2 folds and the genotype TT was established to significantly increase the risk of DN byabout 3 folds with respect to those of the genotype CC. This study concludes that theSNPrs6214 of theIGF-1gene associated with T2DM and DN.]]>
<![CDATA[Socio-demographic Characteristics of Newly Reported HIV Cases in Iraq (1986-2019) ]]>
<![CDATA[Jalil Ibrahim Saleh]]>;
<![CDATA[Nawar Sahib Khalil]]>;
<![CDATA[Haitham Noaman Al-Koubaisy]]>;
<![CDATA[Hussien Ali Mahdi]]>;
<![CDATA[Hadeer Naeem]]>;
<![CDATA[Najoh Kadhim]]>
<![CDATA[ Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology ]]>
Publisher : <![CDATA[Institute of Medico-legal Publications Pvt Ltd ]]>
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DOI: 10.37506/ijfmt.v15i3.15460
<![CDATA[Background: HIV/AIDS is considered as one of the most important public health concern faced by manydeveloped and developing countries.Objective: To study the basic socio-demographic characteristics of the newly reported HIV Iraqi casesduring the period extending from 1986 to 2019.Subjects and Methods: This is across sectional study conducted using data from newly reported HIVIraqi cases from March 2018 to March 2020. These data were available from the patient’s records at theIraqi National HIV/AIDS Program Centre. Annual registration statistics covering the period from 1986 to2019 were reviewed. The diagnosis was done through identification of anti-HIV antibodies using primarilyELISA as screening test and positive cases were confirmed by Western blot assay. The obtained data werecategorized according to the socio-demographic variables and were analyzed statistically.Results: A total number of newly reported HIV Iraqi cases covering the period from 1986 to 2019 was 892cases. The majority of the cases were males (83%), from 21-39 years old (60.3%), alive (65.7%), and single(53.4%). Most of them were from low educational level (45.1%), low socio-economic status (48.3%), andliving in urban areas (75.6%) from Central origin (63.2%).Conclusions: Identification of these findings may provide important directions for targeting certain sociodemographiccharacteristics of the HIV infections to provide specific support for accessing healthcareservices and evaluating the effectiveness of prevention and control measures.]]>
<![CDATA[Phytochemical Study and Isolation of Scopolutin from Pimpinella anisum Seeds Grown in Iraq ]]>
<![CDATA[Noor Mohsen Nasser]]>;
<![CDATA[Widad M K Al-ani]]>;
<![CDATA[Zahraa A.E Al Naqqash]]>
<![CDATA[ Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology ]]>
Publisher : <![CDATA[Institute of Medico-legal Publications Pvt Ltd ]]>
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DOI: 10.37506/ijfmt.v15i3.15462
<![CDATA[As widely known plants are divided into many categories, one of them is the herbs; which have been alwaysused as effective remedies to treat many health issues. One of these remedies belongs to our plant understudy Pimpinella anisum (anise), which belongs to Umbelliferae family, that has been characterized as anaromatic plant used in traditional medicine as carminative, disinfectant, and galactagogue. Anise dry seedswere collected from Iraqi herbal market to investigate their chemical constituents by using two methods:one of them hydrodistillation method using Clevenger apparatus in which oil sample was collected and thenanalyzed by GC/MS and revealed the presence of anethol as the major constituent with percentage of 82.91% of the oil; the second method was alcoholic extraction by Soxhlet apparatus with ethanol till exhaustionthen the extract was concentrated by evaporation under vacuum, after that partition with ethyl acetate wasdone, the ethanol layer was discarded while the ethyl acetate layer was concentrated under vacuum, thecollected fraction were analyzed by TLC comparing it with standard Scopoletin .Phytochemical screeningproved that the plant contain Scopoletin and was confirmed by comparing Rf value of the sample with thestandard in three different solvent systems.]]>
<![CDATA[The Effect of Aqueous Extract of Quercus infectoria Plant on Pap E gene Expression of Uropathogenic Escherichia coli ]]>
<![CDATA[Ammar Jawad Kadhim]]>;
<![CDATA[Hassan Majeed Rasheed]]>
<![CDATA[ Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology ]]>
Publisher : <![CDATA[Institute of Medico-legal Publications Pvt Ltd ]]>
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DOI: 10.37506/ijfmt.v15i3.15463
<![CDATA[One hundred twelve urine samples were collected from Baghdad hospitals and examined by differentidentification techniques. Seventy isolates (62.5%) were diagnosed as Escherichia coli after microscopicand cultural identifications. The result of PCR product electrophoresis on the isolates showed that thirteenisolates (18.57%) have Pap E gene which are uropathogenic E. coli. Antibiotic susceptibility test was done,and four high resistant strains were mixed with aqueous extract of Quercus infectoria plant in 96 well ELISAplate and incubated for different times. After 0, 6, and 12 hr. of incubation, the effect of the plant extract onthe bacterial growth was determined by ELISA reader, and the effect on the expression of Pap E gene wasexamined by real-time PCR. The results were showed that the higher effect of the extract was on the E10strain growth that dropped from 3.184 at zero time of incubation to 2.378 and 2.281 after 6, and 12 hrs. ofincubation respectively. Also, shown a downregulation in the expression of Pap E gene of the isolates atdifferent times of incubation. The E10 strain shows decrease in the fold from 1 before the treatment with theextract to 0.076 at zero time of incubation with the extract, after 6 and 12 hrs. of incubation it shows a totaldown-regulation of the gene.]]>
<![CDATA[The Effect of Using the (K.W.L) Strategy on Developing Inferential Thinking and Performing Some basic Handball Skills for Female Students, College of Education and Sports Sciences, University of Misan ]]>
<![CDATA[Majid Mohammed Msaed]]>;
<![CDATA[Esraa AbdulZahr Irkhayyis]]>;
<![CDATA[Nadia Mahdi Mohammed]]>
<![CDATA[ Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology ]]>
Publisher : <![CDATA[Institute of Medico-legal Publications Pvt Ltd ]]>
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DOI: 10.37506/ijfmt.v15i3.15464
<![CDATA[The importance of research is in the use of modern and effective teaching strategies and methods. Therefore,teachers’ ideas tended to find and search for unique teaching methods. The teacher is a guide and assistantto students on the knowledge and how to use educational techniques and strategies that would enable thestudent from storing knowledge and reusing it in new situations. The present study used the (K.W.L.)strategy in developing inductive thinking and learning some basic handball skills and to gain knowledgefrom the theoretical and practical side. As well as this strategy has a new aspect. It is an expressive methodthat supports teachers to achieve the best results. There were statistically significant differences when usingthe (K.W.L.) strategy in developing inferential thinking and learning basic handball skills in the researchsample. The most prominent conclusions reached by the researchers: firstly the (K.W.L.) strategy hasan apparent effect on developing the performance level of the selected basic skills and stimulating thespirit of competition and plays among the students of the research sample. There is a moral correlationbetween learning some basic skills chosen and between developing inductive thinking among the studentsof the research sample. The most prominent recommendations are the adoption of this strategy by facultyprofessors due to the development achieved in the level of performance. Thus, the increase in suspenseand excitement, and the preparation of this strategy can apply for the rest of the games for the same schoolstage and other stages of the study. As well as the possibility of learning and development for students withinferential thinking, teams for universities can be selected from them for various games.]]>
<![CDATA[Whitening Efficiency of Laser 940 nm and LED (450-505) nm on Extrinsic Stained Teeth (in vitro study) ]]>
<![CDATA[Ali A Hassab]]>;
<![CDATA[Basima Mohammed Ali Hussein]]>;
<![CDATA[Mohammed G Rzoqi G Rzoqi]]>
<![CDATA[ Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology ]]>
Publisher : <![CDATA[Institute of Medico-legal Publications Pvt Ltd ]]>
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DOI: 10.37506/ijfmt.v15i3.15465
<![CDATA[Background: Teeth staining is one of the esthetic problems faced by dentists and patients. Many methodshave been suggested as crowns, restorations and bleaching. Dental bleaching effectiveness depends on manyfactors including technique and materials.Objectives: Studying the effect of diode laser 940 nm and light emitting diode (450-505) nm in bleaching ofstained teeth regarding : whitening efficiency; pulp temperature; surface roughness and topography.Materials and Methods: 40 extracted anterior teeth divided into 6 groups immersed at 37 oC in eithertea; coffee; cola ; juice for 14 days, tobacco stained teeth as well as control group were also included (immersed in distilled water ). These groups were subdivided into laser group or light emitting diode groupbleached according to manufacturer specifications. Shades of teeth were recorded before and after bleaching.Furthermore, temperature changes during bleaching were recorded using thermocouple Thermometer. Allspecimens were examined under light microscope, Atomic Force Microscope and Sectioning ElectronicMicroscope to study the surface roughness and topography.Results: the light emitting diode group yielded slight significant whitening efficiency with more pulptemperature rise (3.4 ºC) compared to the laser group (1.4 ºC). Both groups showed non-significant effect onroughness and topography compared to control group.Conclusion: Although light emitting diode group showed slightly significant shade improvement, but theincrease in pulp temperature was greater than that of laser group indicated better prognosis and safety whendiode laser 940 nm used. Conducting between efficiency and preservation, diode laser is superior in safetyand slightly similar whitening efficiency.]]>
<![CDATA[Serum Level of CCL20 and CXCL 10 in Patients with Vitiligo and their Association with Disease Activity ]]>
<![CDATA[Zainab R. AL-Mousawi]]>;
<![CDATA[Alaa S. Alattabi]]>;
<![CDATA[Dhamiaa M. Hamza]]>;
<![CDATA[Fatima A. AL-Hussiny]]>
<![CDATA[ Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology ]]>
Publisher : <![CDATA[Institute of Medico-legal Publications Pvt Ltd ]]>
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DOI: 10.37506/ijfmt.v15i3.15468
<![CDATA[Aim of Study: study the level of CCL20 and CXCL 10 in serum of patients with vitiligo ,to compare theirlevel in patients with active vitiligo and stable vitiligo and compare level of each cytokine with others Toknow their association with disease activity.Materials/Methods: Sixty-five patients who include (35 active and 30 stable) with vitiligo. All participantsincluded were adult patients with nonsegmental vitiligo.Blood sample were collected by venipuncture from 65 patients (five millimeter of venous were drawing bydisposable syringe under sterilization technique and putting in gel tube then, allowed to clot; after that serumwas separated by centrifugation 1500 rpm for 5 minute. The serum has been collected in plain tube thenstored at -20c to be used for ELISA test to determine concentration of CCL20 and CXCL 10. The resultswere analyzed using the IBM SPSS analytic software.Result: The : current study revealed thatCCL20 are significant (P<0.05), while CXCL10 was not significant(P>0.05) according to the disease activity. The sensitivity and specificity of each of the measured biomarkerswas evaluated using the (ROC). It was found that CCL20 to be of a high significance to measuring thedisease activity. While the CXCL10 was of no significance when compared to the disease activityConclusions: CCL20 could be candidate as a biomarker to evaluate activity of vitiligo]]>
<![CDATA[Mental Skills and their Relationship to Some Basic Skills of Sword Fencing Players for the Middle Euphrates Clubs in Iraq ]]>
<![CDATA[Bushra Hussain Alwan]]>;
<![CDATA[Jabbar Hzoar Mohammed]]>;
<![CDATA[Qahtan Khudhair Abbas]]>
<![CDATA[ Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology ]]>
Publisher : <![CDATA[Institute of Medico-legal Publications Pvt Ltd ]]>
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DOI: 10.37506/ijfmt.v15i3.15469
<![CDATA[The importance of the research lies in identifying the level of mental skills of dueling sword weapon players,due to their close association with the ability to adapt to the different situations required by the strugglesand helps us determine the level of basic skills. Of the sword weapon players, the sword weapon, and theextent of the relationship between them and mental abilities for the purpose of modifying weaknesses anddeveloping strengths, which helps us to stand on an important aspect of the psychological aspects thatsupport the efforts of the trainers and those responsible for the training process in raising the sword weaponplayers to higher levels, and the most important results were not Statistically significant differences appearbetween the players of the research sample teams in all mental skills. The most important recommendation isthe need to work on developing the basic skills of Sword Duel players in a way that ensures advanced levels.]]>
