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Indian Journal of Forensic Medicine & Toxicology
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Core Subject : Health,
Articles 5,497 Documents
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Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15445

Abstract

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Biofilm Forming Bacteria Isolated From Human Eye Conjunctivitis and Keratitis Cases and their Ability to Adhere on Contact Lenses in vitro Mohammed A. Mahdi; Munaff J. Abd Al-Abbas; Ahmed M. Alsamak
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15446

Abstract

One hundred bacteria isolated from conjunctivitis and keratitis showed 14 different bacterial species as Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa were the most. All 100 isolates were tested for their ability to form biofilm, 65 isolates (65%) showed positive for congo red agar (CRA) test with the exception of Enterobacter hormaechei, Staphylococcus haemolyticus and Bacillus pumilus species, while 84 isolates (84%) showed positive for microtiter plate assay except Bacillus pumilusspecies. Nevertheless, icaAD genes were detected in 75 isolates (75%) within all species. Although 100 bacterial isolates showed different results toward biofilm production tests, CRA, MTP assay and icaAD genes, but there was no significant difference found among these tests. Appliedally, the ability to adhere on contact lenses (In vitro) appeared Staphylococcus aureus has the highest adhesion 12(92.3%) followed by P. aeruginosa 3(75%) and Staphylococcus epidermidis 3(60%).
Effect of Exotoxin a Isolated from Pseudomonas Aeruginosa Against Human Cancer Cell Lines Muthanna Hamid Hassan
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15448

Abstract

In This study, the presence of Pseudomonas aeruginosa was investigated from many clinical samples, and then about 54 isolates were obtained, diagnosed with all different diagnostic methods, and then the bacterial isolates produced for Exotoxin A were detected by the ELISA method and then the most productive isolate was chosen for purification by means of Precipitation with ammonium sulphate and ion-exchange column and detection of molecular weight was (M.W 65.03KD).The competent isolation tested its toxicity against two cancer cell lines HeLa and PC3 which were an inhibition ratio 69.1% and 61.6% respectively in high concentration
Forensic Odontology and Its Medico-Legal Applications Sriram Kaliamoorthy; Vijayparthiban Sethuraman; Lakshmanan Vijayalakshmi; Sakthivel Sambasivam
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15449

Abstract

Forensic Odontology is a specialty field of dentistry that analyses dental evidence for the department of justice. Forensic dentistry encompasses all dental specialties and the isolation of this industry from other dental fields is nearly difficult. The objective of the paper is to discuss and describe the applications of forensic odontology in the fields of justice and crime solving.
Management of Patient with Caustic Ingestion Injury: A Case Report Supriadi1; Budi Widodo; Brinna Anindita
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15450

Abstract

The ingestion of caustic substances, both accidental and voluntary, determines a complex syndrome, characterized by severe, often irreversible, visceral lesions, with still remarkable mortality in highly compromised patients. Al36-year-old woman came to Emergency-Room with the chief complaints of blackish vomiting about 4ltimes after drinking liquid to clean the bathroom floor. Patients with findings of grade 2B esophageal injury, pangastritis and gastric ulcer on endoscopy have high the risk of perforation and complications. Endoscopy done within 12lhours and no later than 24lhours following caustic ingestion to classify mucosal injury subsequent to caustic ingestion is useful to determine the severity of injury, particularly in suicidal cases, and thus helpful in predicting outcomes. Patient threat with rehydration with normal saline, total parenteral nutrition, pump omeprazole, injection of methylprednisolone, antibiotic, and sucralfate. Consultation with the Psychiatry department has been conducted psychoeducation and psychotherapy.
Histopathological Changes in Brain Tissues Associated with Oral Administration of Tramadol in Male Rats Abbas Ch. Mraisel; Sawsan A. Ibrahim; Muntadher H. Dawood
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15453

Abstract

Background: Repeated and long treatment with tramadol that could cause toxic metabolites by accumulationin the body and with high level of risk of pharmokinetic interaction alongside the decreasing of tramadol,therefore this study was carried out to investigate the toxic impact of the tramadol on the tissues of the brainin the male rats.Method: This experiment was accomplished and processed at environmental toxicology laboratory,environmental studies department, institute of graduate studies and research, university of Alexandria, Alexandriap) ovince, Egypt. a group of rats (32, male) from Albano waster subspecies and they weigh from 200-250g,all belonging to the animal house of the faculty of medicine, university of Alexandria and grouping in tofour groups (8 rats for each group in each cage). In regards to the control group, they were given basal foodand tap water through-out 10 days by gastric tube, day by day. Group three the rats were fed with basal dietand given Tramadol HCL orally in dose 45mg/ kg .B.W dissolved in (5ml) normal saline (0.9%) by gastrictube, daily for Twenty days. Group for the rats were fed with basal diet and given Tramadol HCL orally indose 45mg/ kg .B.W dissolved in (5ml) normal saline (0.9%) by gastric tube, daily for Thirty days. When theexperimental period reached its end, kidney tissues of each rat were instantly removed and after that weighed thenput into 10 percent of neutral buffer formalin to be considered as a fixative solution.The Results: The results showed significantly decrease in the heaviness of the brain in the groups of therats that given the Tramadol HCL in dose 45mg/ kg .B.W with increasing the time of administration ascompared to the control group. Histopathological changes were observed in rats brain tissues section therats that given Tramadol HCL orally in dose 45mg/ kg .B.W dissolved in (5ml) normal saline for ten daysrevealed mild degree of tissue injury in the cerebral cortex, with few vacuolar degeneration and dilatationof blood vessels, and the tissue sections of group two after ten days revealed mild degree of tissue injury inthe cerebral cortex, with few vacuolar degeneration and dilatation of blood vessels, while the three groupObserved increase in the vacuolar degeneration, with neural atrophy and degeneration of neurons withreduction the neural process and pyknosis of the nuclei dilatation of blood vessels after twenty days oftramadol administration. The tissue Sections the obtained from the group four after thirty days revealedincrease in the vacuolar degeneration, with more atrophy of the neural cells and complete reduction theneural process and pyknosis of the nucleus in the injured neural cells and glyosis.The Conclusion of this study there are harmful toxic effects when administrated the tramadol for longperiod on the brain tissues, therefore abuse of tramadol should be avoided except with medical prescriptionowing to its toxic effects.
The Effect of Moringa oleifera Leaves Powder on Some Hormones to Prevent the Development of Experimental Hypothyroidism in Rabbits Abeer Ataallah Ayyed Al –Hadidy; Suher Taha Dawood
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15454

Abstract

Can Antisperm Antibodies affect Male Fertility after Subinguinal Varicocelectomy ? A Local Study Ehab Jasim Mohammad; Duraid Taha Abdulkareem; Omar Aziz Hassan
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15455

Abstract

Background: Antisperm Antibodies researches emerged in 1899 in a report referring to the ability of spermsto be antigenic in case of injection into a foreign species. In 1900, researchers showed that sperms are alsoantigenic in case of injection into similar species. Moreover, Wilson (1954) identified ASAB in the partnersof infertile male and then observed the association of ASAB with male infertility. In addition, a correlationwas found between ASAB and sperm proteomics, and thus researchers specified those ASABs decreasingmale fertilities via inhibition of the sperm functions crucial for fertilization.Objectives: The study aimed at evaluating the effect(s) of varicocelectomy on the anti-sperm antibody incases who suffer from varicocele.Methods: In the present prospective, controlled, clinical research which has been performed during theperiod from april 2015 to may 2020. Therefore, the blood samples have been randomly provided from190 males with mean age 33 years (ranging from 20-45 years) ,with the clinical varicocele who have beenindicated for varicocelectomy. Then, participants have been randomly selected from the urology outpatientclinic of Al-Yarmouk Teaching Hospital in Baghdad,Iraq,and Al-Ramadi Teaching Hospital and privateclinic.Results: In our study, ASA level (27.2%) with the positive serum ASA prior to with those (43.2%) followingthe operation demonstrated a significant difference statistically (p = 0.033). However, such a result has beennot confirmed in the patients (27.4%) with positive semen ASA prior to with those (31.3%) following thesurgical operation (p = 0.881).Conclusion: According to the results, varicocelectomy did not have any impact on the semen ASA,despite,but serum antibody enhanced following varicocelectomy and sperm motility improved. Therefore,varicocelectomy usefully affected the semen parameters in the infertile men suffering from varicocele.
Prevalence of Dental Erosion among Children aged 11-12 years Old in Primary Schools at Sammawa City(South of Iraq) Shayma Abdullah Hanoon
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15456

Abstract

Objective: The purpose of this research was to assess the prevalence of dental erosion in children betweenthe ages of 11 and 12 years and compare the various factors influencing dental erosion in both study andcontrol groups.Materials and Methods: a clinical assessment of the research was performed among children aged 11- 12years to assess the prevalence of dental erosion in relation to various factors that may cause erosion. Thesample size was 102 children with diagnosed dental erosion(63 boys and39 girls) and saliva from thesechildren was collected using a questionnaire to study the various factors that correlate with dental erosion.Results: the children examined were 1000 aged between 11and 12 years. The prevalence was 10.2 per centmore than girls (3.9per cent) with the highest level in boys(6.3per cent). The salivary flow rate and saliva PHwere lower in the study group compared to the control group and lower in boys compared to girls in the samegroup. Tooth brushing frequencies and carbonated drink intake exhibited a greater effect on the developmentof erosion in study group compared with the control group.Conclusion: in Sammawa city, the prevalence of dental erosion was low and in boys more than girls, andin study group, the salivary flow rate and saliva PH was low. The severity of dental erosion increases withincreased intake of carbonated beverages and tooth brushing frequencies.
Effect of Nigella Sativa (Black Cumin), Vitamin E and their Combination on Metoprolol Induced Hepatotoxicity in Rabbits Fatima Hussein; Jawad Ahmed
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15457

Abstract

Background: Metoprolol (MET) is a selective Beta-1 blocker. It is metabolized in the liver. Unlike mostBetablockers, Metoprolol is unique in causing liver toxicity, which is infrequent, ranging from raised liverenzymes to severe liver damage. Aim: hepatotoxicity was induced in rabbits, and protective effects ofNigella sativa (NS), Vitamin E (Vit E), and a combination of Nigella sativa + Vitamin E were investigatedon Metoprolol-induced hepatotoxicity.Methods: 36 male rabbits were divided into six groups and treated as follow: Metoprolol (20mg/kg),Nigella sativa (2ml/kg), control group, Metoprolol + Nigella sativa, Metoprolol + Vitamin E ( 175mg/kg/day), Metoprolol + Nigella sativa + Vitamin E. Treatments were given orally for two weeks. Liverenzymes, malondialdehyde, glutathione, and interleukins (IL-6, IL-10) were measured in serum and liverhomogenates. Histopathological examinations were done for two animals in each group.Results: Elevated liver enzymes and histopathological changes were seen in all Metoprolol-treated animals.Nigella sativa, Vitamin E, and Nigella sativa + Vitamin E reduced Metoprolol-induced rise in liver enzymes.Metoprolol increased malondialdehyde concentration in serum and liver homogenate. Treatment withNigella sativa, Nigella sativa + Vitamin E insignificantly decreased these levels. Neither Metoprolol norMetoprolol and Nigella sativa, Vitamin E, and their combination changed the levels of IL-6 or IL-10.Conclusion: Metoprolol increased liver enzymes, and treatment with Nigella sativa, Vitamin E, and Nigellasativa + Vitamin E significantly modified rise in liver enzymes. Metoprolol and other treatments had littleeffect on IL-6 or IL-10.