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INDONESIA
Jurnal Fitopatologi Indonesia
ISSN : 02157950     EISSN : 23392479     DOI : -
Core Subject : Health,
Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).
Arjuna Subject : -
Articles 5 Documents
Search results for , issue "Vol 11 No 2 (2015)" : 5 Documents clear
Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae Yadi Suryadi; I Made Samudra; Tri Puji Priyatno; Dwi Ningsih Susilowati; Puji Lestari; Sutoro Sutoro
Jurnal Fitopatologi Indonesia Vol 11 No 2 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.705 KB) | DOI: 10.14692/jfi.11.2.35

Abstract

Microbial secondary metabolites is an important resource for antifungal development in disease control strategy. The objective of this study was to screen Bacillus cereus 11UJ, an antagonistic rhizosphere bacteria for potential secondary metabolite production against rice fungal pathogens, i.e. Rhizoctonia solani and Pyricularia oryzae. The antagonistic effect of crude exract was evaluated using sterile filter paper discs on PDA medium. The ethyl acetate extracts of the bacterium showed a better antifungal activity to P. oryzae than those of R. solani. The inhibitory effect of the filtrate proved the potency of the isolates to produce antifungal. Analysis of pyrolysis gas chromatography-mass spectrometry showed that B. cereus 11UJ produces 3 major compounds i.e; 9,19-cyclolanostan-3-ol, acetate, (3.beta.)- (CAS) cycloartanyl acetate (13.14%); 4-(2’,2’-dimethyl-6’-methyliden-1’-cyclohexyliden)-3-methyl-2-butanone (9.72%); and stigmast-5-en-3-ol oleat (9.09%) which suggested to play an important role in the suppression of rice fungal pathogens.  
Identifikasi Polerovirus Penyebab Klorosis pada Cabai Asal Bali, Indonesia Rita Kurnia Apindiati; Gede Suastika; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol 11 No 2 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (790.801 KB) | DOI: 10.14692/jfi.11.2.43

Abstract

Based on the results of a survey conducted on chilipepper plants in Kertha Village, Payangan Subdistrict, Gianyar District, Bali Province, many chilipepper plants with chlorosis symptoms caused by the virus was observed. Infected plants showed yellowing of the lamina, but the veins remain green. Identification was focused on the Pepper vein yellows virus (PeVYV) through transmission using Aphis nasturtii, observation on the morphology of virus particles, total RNA detection by reverse transcription-polymerase chain reaction (RT-PCR), and nucleotide sequence. The results showed that the disease can be transmitted from infected plant to healthy plant using A. nasturtii. Viral particle has hexagonal shape with the diameter ~30 nm. RT-PCR of total RNA from severe, moderate, and mild symptomatic infected plants, using specific primer sets for coat protein gene of PeVYV successfully amplified DNA with size of ~650 bp. Coat protein gene sequence of PeVYV isolates Bali, Indonesia showing different chlorosis intensity had the highest homology with PeVYV isolates from Taiwan 95.1–96.6%. Based on the data, PeVYV has been confirmed as the cause of chlorosis in chilipepper plants in Bali, Indonesia.
Formulasi Bakteri Endofit Akar Kedelai untuk Pengendalian Pustul Bakteri Trimurti Habazar; Zurai Resti; Yulmira Yanti; Sutoyo Sutoyo; Imelda Imelda
Jurnal Fitopatologi Indonesia Vol 11 No 2 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.147 KB) | DOI: 10.14692/jfi.11.2.51

Abstract

Two isolates of bacterial endophyte from soybean root were found to be effective to control bacterial pustule caused by Xanthomonas axonopodis pv. glycines. Formulation of the bacterial isolates is required to maintain the effectivity of this bacterial isolates during storage, transportation and application. The aim of this research was to obtain the best carrier for formulation to maintain the effectivity of bacterial endophyte in storage to control bacterial pustule on soybean. Three kind of carrier agent was evaluated for formulation of bacterial endophyte i.e. peat soil, tapioca flour and coconut water + 1% palm oil. Each carrier agent was combined with 5 treatment of storage time i.e. 0, 1, 3, 5 and 7 weeks. Soybean plants was inoculated by. X. axonopodis pv. glycines 2 weeks after planting by rubbing bacterial suspension (106 cel mL-1) on lower surface of leaves. The results showed that all formulas of bacterial endophyte were able to suppress the bacterial pustule on soybean. The best formulations were bacterial endophyte in peat soil stored for 1 and 7 weeks (effectivity rate 79.85% and 77.02%) and coconut water + palm oil and storaged for 3 weeks (effectivity rate 77.46%).
Deteksi Virus yang Menginfeksi Kedelai di Jawa Yunita Fauziah Rahim; Tri Asmira Damayanti; Munif Ghulamahdi
Jurnal Fitopatologi Indonesia Vol 11 No 2 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (889.775 KB) | DOI: 10.14692/jfi.11.2.68

Abstract

Virus infection is an important production constraint for soybean. Research was conducted to detect virus infection from soybean samples collected from several locations in Java. Leave samples from 50 plants was taken randomly from each location in Bogor, Cirebon, Bantul, and Ponorogo. Field symptoms was observed and disease incidence was determined based on serological assay using specific antibodies to Cucumber mosaic virus (CMV), Soybean mosaic virus (SMV), Cowpea mild mottle virus (CPMMV), and Bean pod mottle virus (BPMV). Incidence of CMV, SMV, and CPMMV was 72–84%, 14–24%, and 6–8%, respectively; whereas infection of BPMV was not found. Specific viral DNA of CMV, Potyvirus, and Geminivirus was successfully amplified using specific primer for CMV coat protein, universal primer for Potyvirus and Geminivirus, respectively. Nucleotide sequence analysis showed that isolate CMV from soybean has the highest homology (99%) to CMV strain S, Potyvirus isolates has the highest homology (90%) to BCMV isolate Mungbean from China and BCMV strain Blackeye from Vietnam, and Geminivirus isolates has the highest homology (96%) to Pepper yellow leaf curl virus (PYLCV) from Bogor and Java. Phyllogenetic analysis showed that CMV strain S formed
Keragaman Cendawan Rizosfer dan Potensinya sebagai Agens Antagonis Fusarium oxysporum Penyebab Penyakit Layu Tanaman Krisan Engla Yona Liza; Adrinal Adrinal; Jumsu Trisno
Jurnal Fitopatologi Indonesia Vol 11 No 2 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.752 KB) | DOI: 10.14692/jfi.11.2.59

Abstract

Plant rhizosphere is rich of microbes, one of these is a group of fungi. This research aimed to obtain fungi isolates from rhizosphere of healthy Chrysanthemum, followed by evaluation of their potential as biocontrol agents of Fusarium oxysporum. Isolation of fungi rhizosphere was based-on serial dilution method. As many as 6 isolates of rhizosphere fungi was succesfully isolated with diverse characters. Pathogenicity test showed that 6 isolates were not pathogenic because it did not cause wilt symptoms on Chrysanthemum. The selected isolates were subjected to in vitro assay for their potential as biocontrol agents, 4 isolates had antagonist ability (46.42–51.61%) and antibiosis activity against F. oxysporum. Fungi isolates having the potential as biocontrol agents was identified as Trichoderma sp. (2 isolates), Penicillium sp., and Paecillomyces sp.

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