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Hydrogen: Jurnal Kependidikan Kimia
ISSN : 23386487     EISSN : 26563061     DOI : Prefix 10.33394
Core Subject : Science, Education,
Hydrogen: The Chemistry Education Journal published by the Chemistry Education Study Program which contains articles raised from the results of conceptual research and studies in chemistry and chemistry education including education and learning, device development, media and learning models.
Articles 721 Documents
Approaches for Spatholobus littoralis: Comparative Efficiency of Ultrasound- and Microwave-Assisted Extraction Techniques and Its Statistical Analysis Nindya Tri Muliawati; Novia Amalia Sholeha; Mohamad Alief Ramdhan; Farida Laila; Tekad Urip Pambudi Sujarnoko; Wina Yulianti; Atep Dian Supardan; Ika Resmeiliana; Faranita Ratih Listiasari; Hanifa Muthmainna; Khaerunisa Fadillah; Muhammad Afdhal Maliki Akbar
Hydrogen: Jurnal Kependidikan Kimia Vol. 14 No. 3 (2026): June 2026
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v14i3.20353

Abstract

The rising demand for sustainable bioactive materials has expedited the advancement of green extraction technologies sourced from renewable natural resources. This research systematically evaluates Microwave-Assisted Extraction (MAE) and Ultrasound-Assisted Extraction (UAE) for the recovery of bioactive compounds from Spatholobus littoralis Hassk (bajakah root) utilising ethanol. The extraction performance was assessed based on yield, total phenolic content, and physicochemical characteristics. The findings indicated that MAE exhibited superior extraction efficiency and enhanced physicochemical properties relative to UAE. The biological functionality of the extracts was assessed using an anti-termite assay. Bajakah root extracts, derived through MAE, were evaluated at concentrations of 1.5% and 2.5%. A commercial termiticide (Permise®) served as a positive control, while 70% ethanol acted as a solvent control, and untreated samples were designated as a negative control. The anti-termite assay demonstrated that both extract concentrations resulted in complete termite mortality within 2 days, while the positive control took up to 5 days and the untreated group required up to 7 days. The findings indicate the efficacy of S. littoralis root extract as a natural bioactive agent and underscore MAE as a reliable and sustainable extraction method for the advancement of multifunctional green materials.
Molecular Identification of Fungi Isolated from Indonesian Coal using ITS Region Sequence Shinta Wulansari; Fitri Widya Handayani; Muhammad Shobihul Khoir
Hydrogen: Jurnal Kependidikan Kimia Vol. 14 No. 3 (2026): June 2026
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v14i3.20365

Abstract

Fungi represent a highly diverse group of microorganisms found in various environments, including extreme habitats such as coal deposits. This study aimed to identify fungal species isolated from Indonesian coal based on ITS region sequence analysis and compare the effectiveness of CTAB/NaCl and microwave methods for fungal DNA isolation from coal substrates. Fungal isolates were obtained from coal samples using the dilution method on PDA media. DNA isolation was performed using CTAB/NaCl and microwave methods with power levels 700 W. ITS region amplification used PCR with ITS5-ITS4 primers. Sequence analysis was conducted using BLASTn and phylogenetic reconstruction using the Neighbor-Joining method with 1000x bootstrap replicates. Two fungal isolates (isolate A and B) were obtained, with only isolate A further characterized. Morphological observations showed characteristics similar to the genus Penicillium. The CTAB/NaCl method successfully yielded DNA with purity of 2.01 and concentration of 275.89 ng/µL, producing a single DNA band on electrophoresis. Conversely, the microwave method failed to isolate intact DNA across high power, indicated by absence of DNA bands despite spectrophotometric purity values (2.02-3.28) falling within acceptable range. ITS region amplification of isolate A successfully produced a 592 bp product. BLASTn analysis revealed isolate A shared 94% identity with several Penicillium species. Phylogenetic analysis demonstrated isolate A was closely related to Penicillium sublateritium JX841245.1 (94.66% identity; 68% bootstrap support). The identity value below 97% suggests isolate A has potential as a novel or cryptic species within genus Penicillium. This study represents the first report on molecular identification of fungi from Indonesian coal and comparison of DNA isolation methods on this substrate. The CTAB/NaCl method proved superior to the microwave method for fungal DNA isolation from coal. Further analysis with additional genetic markers is needed to confirm the taxonomic status of isolate A.
Pedetan Ethnochemistry Typical of Jembrana and it’s Integration into Green Chemistry Learning Ni Komang Ananda Dian Savitri; I Wayan Suja; Siti Maryam
Hydrogen: Jurnal Kependidikan Kimia Vol. 14 No. 3 (2026): June 2026
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v14i3.20395

Abstract

This study aims to explore the ethnochemical knowledge embedded in the traditional production of Jembrana’s pedetan and to analyze its potential integration into high school green chemistry learning. This research is motivated by the limited ethnochemical studies that highlight local Balinese culinary, especially pedetan, and the absence of studies that specifically link the pedetan production process with the principles of green chemistry as a source of contextual learning. The study uses a qualitative approach with an ethnographic type. The research subjects involved pedetan cooks in Perancak Village, as well as literature sources in the form of books, scientific journals, and the chemistry syllabus of the Merdeka Curriculum. The research objects include local knowledge related to the ingredients and process of making pedetan along with the accompanying chemical phenomena, a study of the chemical content of the ingredients and their benefits, and their suitability with the learning outcomes of high school chemistry. Data collection was conducted through direct observation of the production process, in-depth interviews with informants, and documentation in the form of photographs, field notes, and supporting documents. Data validity was tested through triangulation, reference adequacy, and member checking to ensure consistency with information from informants. The results of the study showed that the main ingredients of pedetan consisted of three types of fish, namely lemuru ( Sardinella lemuru ), tambang ( Sardinella fimbriata ), and layang ( Decapterus ), as well as seven types of plant spices, namely turmeric ( Curcuma longa ), red chili ( Capsicum annuum L. ), galangal ( Alpinia galanga ), coriander ( Coriandrum sativum L. ) , garlic ( Allium sativum L. ), lemongrass ( Cymbopogon citratus ), and aromatic ginger ( Kaempferia galanga L. ). The manufacturing process includes fish cutting, washing, soaking, seasoning, and drying, each of which involves various chemical phenomena. These findings have the potential to be integrated into high school chemistry education, particularly in green chemistry content, through green chemistry principles that align with the pedetan production process. This study recommends the development of broader ethnochemical studies, strengthening through experimental testing, and its use by teachers to support more meaningful contextual learning.
Effect of Blanching on the Protein Profile of Bitter Leaf (Vernonia amygdalina) Analyzed by SDS-PAGE Fitri Widya Handayani; Shinta Wulansari
Hydrogen: Jurnal Kependidikan Kimia Vol. 14 No. 3 (2026): June 2026
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v14i3.20408

Abstract

Blanching is widely employed as a pre-treatment for leafy vegetables to inactivate enzymes and preserve quality. However, its effect on protein integrity in polyphenol rich species remains inadequately characterized. This study evaluated the effect of blanching on the protein profile and protein content of bitter leaf (Vernonia amygdalina). Blanched and non-blanched leaves were extracted using a TCA-based protocol, and protein profiles were analyzed by SDS-PAGE, while protein content was determined by the Kjeldahl method in triplicate. SDS-PAGE revealed distinct protein bands only in blanched samples, whereas non-blanched samples showed no visible bands. Kjeldahl analysis indicated that non-blanched samples had slightly higher protein content (19.20 0.13%) than blanched samples (18.24 0.21%, p < 0.05). These findings suggest that blanching improves protein extractability and electrophoretic detectability, although it slightly reduces total Kjeldahl protein content. Combined qualitative and quantitative approaches are essential for accurate protein assessment in polyphenol-rich plant materials.
The Development of Chemistry E-Module Using STS Approach Based on HOTS on Acid-Base Materials Salma Syauqi; Retno Dwi Suyanti
Hydrogen: Jurnal Kependidikan Kimia Vol. 14 No. 3 (2026): June 2026
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v14i3.20430

Abstract

This study aimed to develop a chemistry e-module using the Science Technology Society (STS) approach based on Higher Order Thinking Skills (HOTS) on acid-base materials for Grade XI students at SMA Negeri 2 Medan. The research employed the 4D development model (Define, Design, Develop, Disseminate). The Define stage identified that chemistry learning—particularly on acid-base topics—was still dominated by conventional methods with limited digital media. The Design stage produced an e-module named KelasKimia.ku, published via Google Sites. In the Develop stage, expert validation was conducted by three validators: content expert (mean score 3.50; Very Feasible), media expert (mean score 3.20; Feasible), and learning expert (mean score 3.30; Feasible). The Disseminate stage involved 71 students across two Grade XI classes. Student response yielded a mean score of 3.60 (Very Feasible). Learning outcomes showed that the experimental class (using the STS-HOTS e-module) achieved a posttest mean of 85.86, compared to 81.39 in the control class. The experimental class obtained an N-Gain of 0.7734 (High category), significantly higher than the control class (0.6971; Moderate). Paired Samples t-Test results showed t = −32.300 with significance 0.000 (< 0.05), confirming that the e-module effectively improved students' HOTS and learning outcomes on acid-base materials.
Designing a Green Chemistry Virtual Laboratory with Project-Based Learning to Improve Acid-Base Titration Outcomes Mutiara Ardi; Manihar Situmorang; Nurul Aliyyah; Emitur Malau
Hydrogen: Jurnal Kependidikan Kimia Vol. 14 No. 3 (2026): June 2026
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v14i3.20567

Abstract

This study developed a Green Chemistry-oriented virtual laboratory integrated with Project-Based Learning (PjBL) for acid-base titration and evaluated its impact on students' learning outcomes and critical thinking skills. The virtual laboratory was designed using the ADDIE model and implemented as a 2D Canva-based application accessible via laptops and smartphones. The study involved 35 experimental and 36 control group students. Validation by three experts resulted in a media feasibility score of 3.30 ± 0.15. The experimental group achieved an average post-test score of 83.31 ± 5.56, significantly higher than the control group's score of 72.36 ± 4.43. Critical thinking skills also enhanced, with an average score of 88.29 ± 4.82. These findings indicate that the Green Chemistry virtual laboratory significantly improves students' learning outcomes and critical thinking skills.
Analysis of High School Students’ Chemistry Learning Motivation Using the Chemistry Motivation Questionnaire (CMQ) Lusiana; Erlina; Eny Enawaty
Hydrogen: Jurnal Kependidikan Kimia Vol. 14 No. 3 (2026): June 2026
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v14i3.20574

Abstract

Learning motivation is an important factor that drives students to achieve success in their studies. This study aims to analyze the level of students' motivation in learning chemistry at SMAN 1 Siding in grades X, XI, and XII who study chemistry using the Chemistry Motivation Questionnaire (CMQ). The research method used is quantitative descriptive with data collection techniques using questionnaires and interviews involving 121 students from four classes, so the population in this study is all students studying chemistry subjects with the sampling technique using saturated sampling. The research results show that the majority of students' motivation to learn chemistry falls into the very high and high categories, with 57% in the very high category for grade X, 59% in the high for grade XI, and 69% in the high for grade XII out of 121 respondents. Based on five aspects of motivation, the three classes tend to score high on the value aspect of motivation. These findings can serve as a basis for teachers in designing more effective learning strategies that align with students' characteristics, so that the already high motivation aspects can be maintained and other motivation aspects can be enhanced to support the success of chemistry learning.
μPADs for Chemistry Education and Acetic Acid Analysis: A Literature Review Medardus Josua Petrus Tosang; Masriani; Firman Shantya Budi
Hydrogen: Jurnal Kependidikan Kimia Vol. 14 No. 3 (2026): June 2026
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v14i3.20631

Abstract

Microfluidic paper-based analytical devices (μPADs) have emerged as promising analytical platforms due to their low cost, portability, simplicity, and minimal reagent requirements. These characteristics make μPADs attractive for both chemical analysis and chemistry education, particularly in laboratory activities that emphasize sustainable and environmentally friendly practices. This literature review aims to examine the development and application of μPADs in chemistry learning and acetic acid determination through acid–base titration experiments. A descriptive literature review with a qualitative approach was conducted using articles published between 2016 and 2025. Relevant literature was identified through Google Scholar, ScienceDirect, PubMed, and ACS Publications using keywords related to μPADs, chemistry education, acid–base titration, and acetic acid analysis. Following a screening and eligibility assessment process, 21 peer-reviewed articles were selected and analyzed using qualitative content analysis. The findings indicate that μPADs serve not only as analytical tools but also as instructional media that support the understanding of acid–base reactions, stoichiometry, limiting reagents, and titration concepts through microscale experimentation. In acetic acid analysis, distance-based and colorimetric μPADs integrated with smartphone-assisted image processing provide results comparable to conventional titration methods while reducing reagent consumption, analysis time, and chemical waste generation. The integration of digital image analysis also introduces students to modern analytical techniques and enhances digital literacy skills. Despite challenges related to fabrication standardization and analytical reproducibility, μPADs demonstrate considerable potential for implementation in higher education laboratories as cost-effective and sustainable tools that support green analytical chemistry practices.
Implementation of STEM-PjBL Teaching Materials to Improve Student Learning Outcomes and Interest in Acid-Base Topics Citra Permata Sari Sinaga; Susilawati Amdayani
Hydrogen: Jurnal Kependidikan Kimia Vol. 14 No. 3 (2026): June 2026
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v14i3.20649

Abstract

Although STEM-integrated Project-Based Learning (PjBL) has been widely examined for its effects on individual learning dimensions, no comparative study has simultaneously investigated its differential impact on both cognitive learning outcomes and affective learning interest using a two-class experimental design in Indonesian secondary chemistry education, particularly for the acid-base topic. Existing studies are further limited by single-variable focus, short-term self-report instruments, and a lack of multi-project STEM designs that integrate cross-disciplinary engineering and mathematical reasoning alongside scientific inquiry. This study addresses these gaps by implementing a STEM-PjBL e-module featuring three differentiated cross-disciplinary projects and comparing its effects against a conventional textbook-based PjBL class. A quasi-experimental pretest-posttest design was employed at SMAN 1 Panombeian Panei during the even semester of the 2025/2026 academic year, with a four-meeting intervention (2 × 45 minutes each). The sample comprised 60 students: 30 in Experimental Class 1 (STEM-PjBL e-module) and 30 in Experimental Class 2 (textbook + PjBL). Within the context of this study, Experimental Class 1 achieved a mean posttest of 80.50 (N-Gain = 0.521, moderate), while Experimental Class 2 achieved 66.00 (N-Gain = 0.266, low). Learning interest in Class 1 increased from 64% to 82% (+18%), compared to 61% to 77% (+16%) in Class 2. Independent Sample t-tests indicated significant differences in outcomes (sig. = 0.000) and interest (sig. = 0.033). Pearson correlation revealed a strong positive association (r = 0.789, sig. = 0.000). These findings suggest that, within this specific context, STEM-PjBL teaching materials with a multi-project design may provide greater pedagogical benefits than conventional textbook-based PjBL.
The Influence of the Teams Games Tournament (TGT) Cooperative Learning Model on Grade X Students’ Learning Outcomes in Learning the Basic Laws of Chemistry at SMAN 1 Sungayang Kurnia Hayati; Mimi Herman
Hydrogen: Jurnal Kependidikan Kimia Vol. 14 No. 3 (2026): June 2026
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v14i3.20687

Abstract

This study was conducted to examine the effect of the Teams Games Tournament (TGT) cooperative learning model on the learning outcomes of tenth-grade students at SMA Negeri 1 Sungayang in the topic of the basic laws of chemistry. The novelty of this research is reflected in the integration of the TGT model with academic games and tournament-based activities designed to enhance students’ engagement and conceptual understanding in chemistry instruction. This study applied a quasi-experimental approach using a nonequivalent control group design. The population comprised all tenth-grade students, with two classes selected as samples through purposive sampling. The experimental group was taught using the TGT cooperative learning model, while the control group received conventional instruction. Data were collected using pretest and posttest instruments that had previously undergone validity and reliability testing. The data analysis included tests of normality and homogeneity, N-gain analysis, and an independent samples t-test. The findings revealed that the experimental class achieved an N-gain score of 0.303, which falls into the moderate category, whereas the control class obtained an N-gain score of 0.089, categorized as low. The hypothesis testing showed a significance value below 0.05 with a t-value of −7.154, indicating a statistically significant difference in learning outcomes between the two groups. These results demonstrate that the TGT cooperative learning model has a significant positive effect on students’ learning outcomes. Furthermore, its implementation promotes active student involvement, collaborative learning, increased motivation, and deeper conceptual understanding, which collectively contribute to improved academic achievement.

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