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INDONESIA
Bioscientist : Jurnal Ilmiah Biologi
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Articles 97 Documents
Search results for , issue "Vol 11, No 2 (2023): December" : 97 Documents clear
Analisis Kesulitan Belajar Siswa Kelas X IPA di SMA Negeri 3 Tebas pada Materi Protista Hisdayu Hisdayu; Titin Titin; Andi Besse Tenriawaru
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9347

Abstract

Biology is considered difficult for students to study, this condition cannot be separated from the nature of science. Studying science is complex because it emphasizes unification, systematization, combination and analysis of information and concepts. The aim of this research is to measure the level of learning difficulties experienced by class X Science students on Protista material. The method used is a case study. The research subjects were students of class X Science at SMA Negeri 3 Tebas, Sambas Regency. Data collection instruments consist of diagnostic tests, questionnaires and interviews. The results of the research showed that the learning difficulty of class X Science students at SMA Negeri 3 Tebas on Protista material was 91.94% with very high criteria. The factors that influence students' learning difficulties are internal factors of 78.18% with strong criteria, and external factors of 64.14% with strong criteria.
Penggunaan Ekoenzim pada Tingkat Pertumbuhan Bayam Cabut (Amaranthus viridis) Yewi Eltiya; Nirwana Nirwana; Deni Parlindungan; Mellyta Uliyandari; M. Sutarno
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8664

Abstract

Spinach is one of the most common types of green plants in Indonesia. This one plant is a type of vegetable that is easily processed for everyday food, from soup, pecel, gado-gado (Indonesian food), also chips. Amaranthus viridis also known as spinach pull out. In this study, observed the growth of spinach plants with ecoenzyme treatment. The treatment groups were: A (1.5% ecoenzyme), B (3% ecoenzyme), C (4.5% ecoenzyme), D (6% ecoenzyme), E (control/without treatment), F (NPK/ chemical fertilizer). Parameters observed were height, stem diameter, number of leaves, leaf diameter, wet weight and color. This type of research is experimental research and ANOVA data analysis techniques. The duration of the study was 27 days with 9 scheduled observations. Measurements were made in the afternoon, with reference to the observation parameters of stem height, leaf diameter, number of leaves, leaf diameter, leaf color and wet weight. The results of the observations showed that the ecoenzyme treatment was no better than the control and the NPK fertilizers showed that the height, stem diameter, number of leaves, leaf diameter, wet weight and color tended to be smaller. The conclusion of giving ecoenzymes at doses of 1.5%, 3%, 4.5% and 6% does not accelerate and tends to inhibit the growth of spinach (Amaranthus viridis).
Pengaruh Larutan Hara dan Pupuk Organik Cair Urin Kelinci terhadap Pertumbuhan Pakcoy (Brassica rapa L.) pada Teknik Hidroponik Selesa Iqlima; Diah Rachmawati
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8957

Abstract

The demand for pakcoy (Brassica rapa L.) a nutritious vegetable is increasing without being balanced due to the limited of agricultural land. The Wick system hydroponic cultivation technique can be a solution to increase pakcoy productivity. AB mix hydroponic nutrient solution is difficult to reach because it is expensive and has a negative impact on plants. It can be combined with rabbit urine LOF to provide essential nutrients for plants. This research aims to determine the effect of the combination of AB mix with LOF rabbit urine on the growth of pakcoy plants. The research design is CRD, 1 factor, 8 treatments, and 3 replications. Treatments include A1P0=AB mix 100% +0mL/L LOF rabbit urine, A1P1=AB mix 100% + 1mL/L LOF rabbit urine, A1P2=AB mix 100%+2mL/L LOF rabbit urine, A1P3=AB mix 100% +3 mL LOF rabbit urine, A2P0=AB mix 75%+0mL LOF rabbit urine, A2P1=AB mix 75%+1mL/L LOF rabbit urine, A2P2=AB mix 75%+2mL/L LOF rabbit urine, and A2P3=AB mix 75%+3mL/L L of rabbit urine.The addition of rabbit LOF gave the best results with AIP3 treatment on growth parameters, plant height, number of leaves, leaf area, fresh weight of shoots, and roots, dry weight of shoots and roots, respectively 27.41 ± 1.44 cm, 17.33 ± 0.95 pieces 632.89±228.20cm2, 61.88±13.42g, 1.648±0.374g, 3.132±0.614g, and 0.101±0.021g. The essential nutrient content of rabbit urine LOF increases the availability of internal nutrients which are influenced by metabolism, absorption capacity, water and sunlight.
Induksi Akar Anggrek Dendrobium lasianthera dengan Pemberian Beberapa Konsentrasi Naphthalene Acetic Acid (NAA) Secara In Vitro Naura Muthiah Arli; Zozy Aneloi Noli
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9289

Abstract

Dendrobium lasinthera is an orchid that has considerable potential as a raw material for anti-cancer drugs and for the production of cut flowers. Therefore, this orchid is a plant with high economic value. This orchid has beautiful and long-lasting flowers. The demand for this orchid is increasing along with its diverse uses, so the propagation of this orchid needs to be increased. In vitro propagation is the most appropriate step to reproduce this orchid. One of the stages that determines the success of orchid propagation through tissue culture is the root induction stage before acclimatization. Using the right type and concentration of Growth Regulatory Substances (ZPT) is very important. This research aims to determine the effect of several NAA Growth Regulatory Substance (ZPT) concentrations and determine the best NAA concentration for Dendrobium lasianthera root induction. This study used a Completely Randomized Design (CRD) with several NAA concentrations (Control (A); 0.5 mg/L (B), 1 mg/L (C), and 1.5 mg/L (D)), on Murashige & Skoog (MS) media. The results of this study show that NAA has a significant influence on the percentage of root formation, root emergence time, and root length in the Dendrobium lasianthera orchid. NAA 1.5 mg/L is the best concentration for induction of Dendrobium lasianthera orchid roots.
Pengaruh Pemberian Beberapa Jenis Yogurt Komersial terhadap Jumlah Profil Darah Tikus (Rattus norvegicus) yang Diinduksi Diabetes Melitus Husnarika Febriani; Syarifah Widya Ulfa; Tri Novitashari Butar-Butar
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9398

Abstract

Diabetes mellitus is a metabolic disorder that occurs due to inadequate insulin levels or insulin resistance which leads to hyperglycemia. Handling hyperglycemia conditions in diabetes mellitus is very necessary, one of which is by consuming healthy and nutritious food. The aim of this research is to determine the results of total antioxidant levels in types of yogurt which can have an influence on the blood profile of white rats (Rattus norvegicus) with diabetes mellitus. This research used white rats (Rattus norvegicus) as experimental animals which were divided into 5 groups (K+: normal type, K-: negative type (alloxan), P1: type I yogurt brand (drinking yogurt), P2: type. yogurt brand II (yogurt set), P3: kel. yogurt brand III (stirred yogurt) with 5 treatment repetitions each. Yoghurt was given using the oral sonde technique to the experimental animals. Blood samples from the experimental animals were taken through the orbital sinus using hematocrit. Profile Rat blood was tested using a hematology analyzer. The data obtained were analyzed using one-way ANOVA and Duncan's test with a significance level of 0.05. Statistical results showed that there were significant differences (p < 0.05) in each treatment group. Based on the average results erythrocytes, hemoglobin, leukocytes and platelets that brand III yogurt samples had a better level of improvement than the others. This is also in line with the results of testing antioxidant levels in brand III yogurt, namely 51.38 ppm, which means the antioxidant levels are strong.
Pengaruh Ekstrak Metanol Buah Lakum (Cayratia trifolia L. Domin) terhadap Nilai SGOT dan SGPT Tikus Putih Diinduksi Parasetamol Diah Wulandari Rousdy; Elvi Rusmiyanto Pancaning Wardoyo; Catur Wulandari
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8530

Abstract

Lakum fruit (Cayratia trifolia L. (Domin)) has secondary metabolite compounds that are beneficial to health, one of which is a hepatoprotector. This study was conducted to prove the potential of hepatoprotective activity of lakum fruit methanol extract based on the value of SGPT and SGOT enzyme. The research method used a pre-posttest randomized control group design with a sample of 35 male rats divided into five treatment groups: solvent control group (CMC 0.5%); positive control group (HEPA-Q®); treatment group extract (0,575; 1,15; 1,725 mg/g BW). The treatment of induction of paracetamol and methanol extract of Cayratia trifolia fruit was carried out for 7 days respectively. The results showed that the dose of 1,15 mg/g BW of Cayratia trifolia fruit methanol extract had the most effective deterioration of SGOT and SGPT values with a percentage reduction of SGOT of 49.3% and SGPT of 51.8% (p <0.05) which approached control positive (HEPA-Q®). Based on the research conducted, methanol extract of Cayratia trifolia fruit has the potential to be hepatoprotective.
Aktivitas Penghambatan Ekstrak Etanol Biji Asam Gelugur (Garnicia atroviridis) terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus Haris Muntaha; Sunardi Sunardi; Ngatirah Ngatirah
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8142

Abstract

This study aims to determine the inhibitory activity of ethanol extract from tamarind seeds against the growth of Escherichia coli and Staphylococcus aureus bacteria at various concentrations. This study used a randomized complete block design with 1 factor, namely the concentration of tamarind seed extract with 6 levels, namely A1 (0.5%), A2 (1%), A3 (1.5%), A4 (2%), A5 (2.5%), A6 (3%). Each treatment was repeated 3 times. Research evaluation includes qualitative phytochemical tests and antioxidant activity against ethanol extract of gelugur acid seeds and bacterial inhibition. The results showed that in the ethanol extract of tamarind seeds there are phytochemical compounds which include flavonoids, saponins, tannins, and terpenoids. And has antioxidant activity of IC 50 = 15.77 ppm. Tamarind seed extract affects the growth of Escherichia coli and Staphylococcus aureus bacteria. The minimum concentration of ethanol extract of tamarind seeds that can inhibit Escherichia coli and Staphylococcus aureus bacteria is 0.5% with inhibitory zones of 15.6 mm and 10.9 mm respectively.
Etnobotani Bahan Makanan Tradisional di Desa Bonjeruk Kecamatan Jonggat Kabupaten Lombok Tengah Rosalina Edy Swandayani; Arista Suci Andini; Meilinda Pahriana Sulastri
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.7900

Abstract

Ethnobotany studies direct relationship between humans and plants that are used traditionally, one of which examines the use of plants as ingredients in culinary. West Nusa Tenggara Province (NTB) is a province in Indonesia with a distinctive culture including a variety of cuisines, one of which is in Bonjeruk Village, Jonggat District, Central Lombok. The culinary culture of the Sasak people use of plants as their main ingredients. In addition, this traditional food is the potential for promotion to support tourism. The purpose of this study was to inventory and identify the types of plants used for traditional food ingredients as a basis for developing local culinary-based tourism in Bonjeruk Tourism Village. This research is also expected to be able to document and preserve knowledge related to traditional food before this knowledge becomes extinct. The research was conducted in October-December 2022. The selection of subjects was carried out using the snowball sampling method and interviews were conducted using an in-depth interview technique. Identification of plants is done by way of inventory through direct observation. Based on the research results, it was found that 40 species were used in 23 families. The types of plants are utilized in 22 traditional food menus.
Performa Rapid Diagnostik Tes Hepatitis B dengan ELISA sebagai Gold Standar I Gusti Ayu Sri Andayani; Mohammad Rizki; Made Sriasih; Ika Nurfitria Tauhida
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9035

Abstract

The ELISA (Enzyme Linked Immunoassay) method has high sensitivity and specificity, qualitatively and quantitatively detecting Hepatitis B Surface Antigen (HBsAg) levels, but the process is long, expensive and requires special expertise, compared to the Rapid Diagnostic Test (RDT) as a rapid test which is more practical and cheaper. The aim of this study was to compare the sensitivity and specificity of the Entebe Rapid Test Diagnostic (ERTD) produced by PT. Hepatika Mataram, with ELISA as the gold standard in detecting HBsAg. The requirement for interpreting sensitivity and specificity results according to World Health Organization (WHO) standards is a minimum of 95%. Testing with a negative panel of 00 samples and a positive panel of 1 samples from Papua in 012 at -80℃ storage. Testing in April 023 at the Molecular Diagnostics and Research Laboratory, Mataram University Hospital. Statistical analysis using MedCal software. ERD HBsAg produced by PT. Hepatika Mataram has a sensitivity of 95.45%, specificity of 99.50%, positive predictive value of 93.59%, negative predictive value of 99.65%, and accuracy of 99.21%.
Pengaruh Aplikasi Nanobiostimulan Rumput Laut (Padina minor Yamada) terhadap Kadar Klorofil Kedelai (Glycine max (L.) Merr.) Millania Putri Shayen; Zozy Aneloi Noli; Tesri Maideliza; Suwirmen Suwirmen
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9063

Abstract

Chlorophyll is a green pigment in plants which is important for the process of photosynthesis. The photosynthesis process affects plant growth. Seaweed extract has been proven to be a source of biostimulants that can increase plant growth, one of which is Padina minor. Concentration and frequency are important factors that influence the work of biostimulants. One effort to increase the effectiveness of biostimulants is by changing the crude extract into the form of nanoparticles. This research aims to analyze the effect of concentration, frequency, and interaction between concentration and frequency of crude extract and Padina minor nano extract on chlorophyll levels in soybean plants. The method used was a completely randomized design (CRD) in factorial consisting of 2 factors and 4 replications. Factor A concentration of Padina minor extract, namely control, crude extract 0.4%, nano extract 0.2%, 0.3%, 0.4%, 0.5% and 0.6%. Factor B is the frequency of administration of Padina minor extract, namely 1, 2, and 3 times administration. Based on research that has been carried out, the results showed that administration of 0.4% crude extract increased the average chlorophyll a level by 6.6% and a total of 11%. Meanwhile, chlorophyll b gave the same results as 0.3% nano extract, namely an increase of 14% compared to the control. The total frequency of administration of Padina minor extract was 3 times, and the same as the other treatments, increasing chlorophyll a levels by 4.6%, b by 5.2%, and a total of 4.9% compared to the control. The interaction between concentration and frequency of administration of Padina minor nano extract gives the same results as the crude extract, with lower concentration and less frequency of application in increasing levels of chlorophyll a, chlorophyll b, and total chlorophyll in soybean plants.

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