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Jurnal Sains dan Teknologi Laboratorium Medik
Published by Akademi Kesehatan John Paul II Pekanbaru
ISSN : 25275267     EISSN : 26217708     DOI : https://doi.org/10.52071/jstlm
Core Subject : Health, Science, Education,
Education Health Professions Immunology & microbiology Public Health Other
Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM, e-ISSN: 2621-7708, p-ISSN: 2527-5267) adalah sebuah jurnal yang didedikasikan untuk plublikasi hasil penelitian yang berkualitas dalam bidang sains dan laboratoium medik. semua publikasi di jurnal Jstlm bersifat akses terbuka yang memungkinkan artikel tersedia secara bebas online tanpa berlangganan apapun. Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM) dikelola secara profesional dalam hal membantu para akademisi, peneliti dan praktisi untuk menyebarkan hasil penelitiannya. focus dan scope Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM) adalah sains, mikrobiologi, parasitologi, hematologi, kimia klinik, dan toksikologi.
Arjuna Subject : Umum - Umum
Articles 94 Documents
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ANALISIS KADAR ASAM OKSALAT PADA BAYAM BERDASARKAN SUHU PENYAJIAN Sari, Mayang Fadma
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 2 (2023): November 2023
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i2.128

Abstract

Spinach is one type of vegetable that contains a myriad of nutrients, but behind the many benefits contained in spinach there is also an inhibitory substance in the form of oxalic acid which is a chemical that can cause kidney stones, not only that excess oxalate in the body will also cause deficiency. or calcium deficiency. The purpose of this study was to determine the levels of oxalic acid in red and green spinach boiled water with different serving temperature variations. This type of research is descriptive, which is research conducted with the aim of describing or describing facts about the population systematically and accurately. With a total sample of 180 grams divided by 90 grams of red spinach and 90 grams of green spinach. The sampling technique used is Simple random sampling with a qualitative examination permanganometric titration method. The variable in this study was the oxalic acid content in red and green spinach based on serving temperature, with univariate data analysis. The results showed that the levels of oxalic acid in red spinach with temperature variations of 80oC, 60oC to 40oC were 16.84mg/l, 19.28mg/l, 20.05mg/l. and green spinach with a temperature variation of 80oC, 60oC to 40oC is 16.50mg/l, 17.68mg/l. 19.39mg/l. In this study, it can be concluded that the content of oxalic acid in boiled water of red spinach and green spinach based on the serving temperature of 80oC, 60oC to 40oC has increased but is still at levels that are safe for consumption.
GAMBARAN BERBAGAI MEREK HAND SANITIZER DALAM MENGHAMBAT PERTUMBUHAN BAKTERI Staphylococcus Aureus SECARA IN-VITRO elbetan, sabri
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 2 (2023): November 2023
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i2.148

Abstract

Clean and Healthy Living Behaviour (PHBS) as an improvement in public health, one of which is washing hands or maintaining hand hygiene. Can be done in addition to using clean water can also use Hand sanitiser. In the community there are many brands of hand sanitiser, and this needs to be proven for its effectiveness. One of the things that can be done is to test its effectiveness on a laboratory scale. Bacteria that are commonly found as floranormal bacteria are Staphylococcus aureus. On a hospital scale this bacterium becomes a nosocomial problem as a methicillin-resistant bacterium or MRSA. The purpose of the study was to determine the effectiveness of various brands of hand sanitiser against the growth of Staphylococcus aureus ATCC 29213 bacteria. Test method using Kirby Baure with Mannitol Salt Agar media. Samples included 3 brands of Hand sanitiser which were carried out with 5 repetitions. The results of the study were that the 3 Hand sanitisers tested had various inhibition zones in inhibiting the growth of Staphylococcus aureus bacteria. The results of the Hand sanitizer inhibition test against the growth of Staphylococcus aureus, which is more effective is Hand sanitiser A than B and C, with a diameter of 19.66 mm, std. deviation 2.66 which indicates that type A Hand sanitiser is more effective and the distribution of its influence is more evenly distributed. Normality test results obtained Sig value. >0.05 the data is normally distributed, the results of the normality data test value Sig. >0.05 homogeneous data variance, One Way Anova test Sig. <0.05 there is a real difference, Post Hoc test results Hand sanitizer B and C Sig value. <0.05 there is a real difference.
The GAMBARAN EFEKTIVITAS ANTISEPTIK TERHADAP PERTUMBUHAN Candida albicans Ndara Milla, Paul Hazler
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 2 (2023): November 2023
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i2.149

Abstract

Antiseptics are antimicrobial substances that aim to inhibit or damage microorganisms on the surface of a living tissue. Candida albicans is the cause of candidiasis. This antiseptic has a role as an antimicrobial. The purpose of the study was to determine how minimal antiseptic concentrations inhibit the growth of Candida albicans and to determine how much antiseptic concentration kills the growth of Candida albicans. Type of experimental research using antiseptic samples. Solid dilution method Pour cup technique. The results of the study on antiseptic A concentrations of 20% (140 x 102 Cfu/ml), 40% (40.5 x 102 Cfu/ml), 60% (38 x 102 Cfu/ml), 80% (0 colonies), 100% (0 colonies). At antiseptic B concentration 20% (144.5 x 102 Cfu/ml), 40% (52 x 102 Cfu/ml), 60% (38 x 102 Cfu/ml), 80% (0 colonies), 100% (0 colonies). At antiseptic C concentration 20% (145.5 x 102 Cfu/ml), 40% (22 x 102 Cfu/ml), 60% (20 x 102 Cfu/ml), 80% (0 colonies), 100% (0 colonies). The conclusion of this study is a decrease in the number of Candida albicans colonies in antiseptic A concentration of 60% (38 x 102 Cfu/ml) antiseptic B concentration of 60% (38 x 102 Cfu/ml), antiseptic C concentration of 60% (20 x 102 Cfu/ml). Concentrations where there is no growth of Candida albicans colonies are found in antiseptic A, B and C concentrations of 80% (0 colonies).
Pemeriksaan Kadar HbCO Pada Relawan Pengatur Jalur Putar Balik Di Jalan Menggunakan Metode Spektrofotometer Uv-Vis Ardani, Rifky
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 2 (2023): November 2023
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i2.154

Abstract

The work of volunteers managing the U-Turn Route is very vulnerable to exposure to carbon monoxide, if it is inhaled by the body it will become CarboxyHemoglobin (HbCO). The aim of this research is to determine the levels of HbCO in volunteers' blood which regulates the U-turn pathway. The research population was 8 male people and the samples were veins. The results of this study showed that the lowest average HbCO level in the respondents' blood was 4.56% and the highest was 8.10%. Based on the age of respondents, 20-35 years old had an average level of 7.1%, aged 35-45 years had an average level of 5.8%. Based on work experience of more than 1 year, the average level is 6.2%, work experience of less than 1 year has an average level of 6.5%. Based on working time of 0-3 hours, it has an average level of 7.1%, working time of 4-7 hours has an average level of 5.6%. This research uses the Shapiro Wilk test and hypothesis testing using the correlation test with a significant value = α < 0.05. and obtained significant results, namely 100% abnormal.
Analisis Hasil Pemeriksaan Kreatinin pada Sampel dengan Penambahan Hemolisat Berbagai Level Putri, Salsa Amelia
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 2 (2023): November 2023
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i2.158

Abstract

Examination of creatinine levels in the blood is one of the parameters used to assess kidney function because the concentration in plasma and its excretion in the urine in 24 hours is relatively constant. Hemolyzed plasma samples can affect creatinine levels with the examination results tending to decrease when compared to normal plasma examination results. Objective: To assess the percentage decrease or increase in mean, precision, accuracy, and clinical differences in creatinine assay by Jaffe Reaction Method using plasma samples with various levels of hemolysate added against samples without hemolysate added. Methods: Pure experiment (true experimental) with research samples in the form of venous blood of students and female students of the D-3 Medical Laboratory Technology Study Program of the East Kalimantan Ministry of Health Polytechnic as many as 40 samples. This research was conducted at the Hematology Laboratory and Clinical Chemistry Laboratory of the Poltekkes Kemenkes Kalimantan Timur. Data were obtained through measurement of the object of research after being treated in the form of the addition of various levels of hemolysate. Data analysis using Microsoft Excel application. Results: The mean decrease in creatinine test results of insignificant hemolysis and mild hemolysis samples against nonhemolyzed plasma samples was 6% and 8%. Accuracy value <5% in creatinine examination of insignificant hemolysis and mild hemolysis samples by -5% and -6%. The precision value >6% in the creatinine examination of insignificant hemolysis and mild hemolysis samples was 13.6% and 15.6%. Total error >15% TEa in the examination of insignificant hemolysis and mild hemolysis hemolysis samples was 27.2% and 31.2%. Conclusion: all the results of data analysis are outside the normal range and have a false decrease due to the addition of various levels of hemolysate, so the variation of insignificant hemolysis and mild hemolysis samples cannot be accepted or used in creatinine examination parameters.
Uji Efektivitas Daun Bawang Dayak (E. Palmifolia) Menggunakan Pelarut Etil Asetat Terhadap Pertumbuhan Staphylococcus Aureus Dengan Metode Sumuran Jumaqairiah, Siti
Jurnal Sains dan Teknologi Laboratorium Medik Vol 10 No 2 (2024): November 2024
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v10i2.162

Abstract

Dayak leek (Eleutherine palmifolia) is one of the plants that is often used by people for traditional medicine in the Kalimantan region. Based on research, Dayak leeks contain alkaloids, flavonoids, saponins and tannins which can function as antibiotics.The aim of this research is to determine the effectiveness of Dayak leeks (Eleutherine palmifolia) using ethyl acetate solvent on the growth of Staphylococcus aureus using the well method. The research design used was true experimental, where this study used a treatment group and a positive control group (clindamycin 2 mcg).The results of the research showed that in the test Dayak leek extract with concentrations of 60%, 65%, 70%, 75% and 80% formed an average diameter of the inhibition zone of 2 mm, 3.8 mm, 4 mm, 4.4 mm and 7 mm in a row. And the control material clindamycin 2 mcg obtained an inhibition zone of 14 mm. So it can be concluded that Dayak leeks can be used as an alternative to antibiotics, especially at high concentrations.Keywords: Dayak leek extract, Staphylococcus aureus, inhibitory power
GAMBARAN KADAR CARBOHYDRATE ANTIGEN 19-9 ( CA 19-9 ) PADA PASIEN YANG DI CURIGAI KANKER PANKREAS DI RSUD ABDUL WAHAB SJAHRANIE SAMARINDA PADA TAHUN 2020-2022 Pondi, Restiyani
Jurnal Sains dan Teknologi Laboratorium Medik Vol 10 No 2 (2024): November 2024
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v10i2.165

Abstract

Pancreatic cancer is one of the most fatal cancers and the leading cause of cancer-related deaths. The survival rate for pancreatic cancer in the United States is reported to be 8%, which is another common type of cancer and in Indonesia itself pancreatic cancer is ranked 17th. Ca 19-9 is a cell produced by pancreatic exocrine gland cancer cells and can be detected in blood tests. The purpose of this study was to determine the Description of Ca 19-9 Levels in Pancreatic Cancer Patients at Abdul Wahab Sjahranie Hospital, Samarinda in 2020-2022. The research design used was the type of Analytical Observational method using Cross Sectional, meaning that the variables studied were only imposed once on a number of subjects who were the research samples. The results of the study were collected from medical records in the clinical pathology laboratory at Abdul Wahab Sjahranie Hospital, Samarinda in 2020-2022. From this study, it was found that there were 72 patients suspected of pancreatic cancer, where the majority were <55 years old, and 57% were male. Furthermore, based on the examination of Ca19-9 levels, it was found that 28 patients experienced levels above normal as much as 39%. The conclusion is that patients suspected of pancreatic cancer who underwent Ca 19-9 examination for the first time obtained results above normal levels, there were 28 patients with a percentage of 39% with an average age of > 65 years.
Perbedaan Sampel Hemolisis dan Non Hemolisis terhadap Nilai Prothombin Time (PT) pada Mahasiswa Poltekkes Kaltim Nashirah, Najwa
Jurnal Sains dan Teknologi Laboratorium Medik Vol 10 No 2 (2024): November 2024
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v10i2.167

Abstract

Haemolysis is the rupture of erythrocyte cells which results in the erythrocytes releasing haemoglobin into the serum or plasma. Haemolysed samples will affect pre-analytical errors in many laboratories. According to the Clinical Laboratory and Standards Institute (CLSI), in the Prothombin Time (PT) and Activated Partial Thromboplastin Time (APTT) testing guidelines, haemolysed samples should not be used for examination due to the potential activation of clotting factors and interference at the end of the measurement point. The purpose of this study was to determine the difference in the results of Prothombin Time (PT) examination in haemolysis samples with non-hemolysis. This type of research is a quasi-experimental design. The research design used is Nonequivalent Control Group Design, which is a design that takes measurements in treated and untreated groups. The samples used in this study were blood sodium citrate haemolysis and non-hemolysis as many as 39 specimens. Data normality analysis using Shapiro Wilk. Paired T-Test test to determine the difference between haemolysis and non-haemolysis samples. The results of this study showed that the average Prothombin Time value of non-hemolysis samples was 15 seconds, mild hemolysis samples were 9 seconds and moderate hemolysis was 8 seconds. In the univariate test results, the results were shortened in mild haemolysis and moderate haemolysis samples. In the bivariate test results obtained normal distributed results and obtained there is a difference in Prothombin Time values in hemolysis and non-hemolysis samples. The conclusion of this study is that there is a significant difference in hemolysis and non-hemolysis samples on the value of Prothombin Time with p value <0.05. Keywords: Haemolysis, Photo Optic, Prothombin Time
Identifikasi Angka Kuman Udara Pada Ruang Pelayanan Di Puskesmas Wonorejo Samarinda nathania, Nhazwa
Jurnal Sains dan Teknologi Laboratorium Medik Vol 10 No 2 (2024): November 2024
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v10i2.169

Abstract

Puskesmas merupakan pusat pelatihan tenaga kesehatan sebagai sarana pelayanan kesehatan, sehingga berpotensi menjadi tempat penularan penyakit dan memungkinkan pencemaran lingkungan. Kualitas udara yang tidak memenuhi persyaratan sebanding dengan angka kuman dalam ruang akan menyebabkan infeksi nosokomial. Tujuan penelitian untuk mengidentifikasi total angka kuman udara pada ruang pelayanan Puskesmas Wonorejo Samarinda. Jenis penelitian adalah deskriptif dengan metode settle plate. Sampel yang digunakan adalah ruang pemeriksaan lansia, ruang pemeriksaan balita dan ruang tindakan pada tanggal 19 Maret – 23 Maret 2024. Hasil penelitian hari pertama, angka kuman pada ruang balita 713 CFU/m3, ruang lansia 918 CFU/m3, ruang tindakan 346 CFU/m3. Hari kedua, ruang balita 692 CFU/m3, ruang lansia 805 CFU/m3, ruang tindakan 437 CFU/m3. Hari ketiga, ruang balita 649 CFU/m3, ruang lansia 791 CFU/m3, ruang tindakan 430 CFU/m3. Hari keempat, ruang balita 289 CFU/m3, ruang lansia 480 CFU/m3, ruang tindakan 282 CFU/m3. Hari kelima, ruang balita 466 CFU/m3, ruang lansia 346 CFU/m3, ruang tindakan 261 CFU/m3. Kesimpulan penelitian adalah angka kuman udara pada ruang balita dan ruang lansia dari hari pertama hingga ketiga belum memenuhi syarat. Namun, hari keempat dan kelima memenuhi syarat. Sedangkan, angka kuman udara pada ruang tindakan dari hari pertama hingga kelima memenuhi syarat sesuai PERMENKES No.7 Tahun 2019,yaitu 200-500 CFU/m3.
ANALISIS KADAR TIMBAL (Pb) PADA PEKERJA PENGELASAN DI DAERAH SAMARINDA SEBERANG DENGAN METODE SPEKTROFOTOMETER SERAPAN ATOM (SSA) Wijaya, Fransiska Angeli Wijaya Angeli
Jurnal Sains dan Teknologi Laboratorium Medik Vol 10 No 2 (2024): November 2024
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v10i2.174

Abstract

Welding is a process of permanently joining metals by heating the metal to the melting point. This process will cause the accumulation of heavy metals in the air. UV and IR radiation is a kind of environmental exposure that can occur during welding. Long-term exposure to welding rays can cause problems with the lens of the eye including loss of transparency, discomfort in activities, itching in the eye area and breathing. Lead that is inhaled and enters the lungs will be associated with blood. Lead that is absorbed by the body will bind to red blood cells and then be distributed into the blood, extracellular fluid, and some soft places (liver, kidneys, and nerves) and mineral tissues (bones and teeth). The purpose of this study was to determine the blood lead levels of welding workers based on length of work, age, and working period with a normal limit value of

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