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Jurnal Sains dan Teknologi Laboratorium Medik
Published by Akademi Kesehatan John Paul II Pekanbaru
ISSN : 25275267     EISSN : 26217708     DOI : https://doi.org/10.52071/jstlm
Core Subject : Health, Science, Education,
Education Health Professions Immunology & microbiology Public Health Other
Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM, e-ISSN: 2621-7708, p-ISSN: 2527-5267) adalah sebuah jurnal yang didedikasikan untuk plublikasi hasil penelitian yang berkualitas dalam bidang sains dan laboratoium medik. semua publikasi di jurnal Jstlm bersifat akses terbuka yang memungkinkan artikel tersedia secara bebas online tanpa berlangganan apapun. Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM) dikelola secara profesional dalam hal membantu para akademisi, peneliti dan praktisi untuk menyebarkan hasil penelitiannya. focus dan scope Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM) adalah sains, mikrobiologi, parasitologi, hematologi, kimia klinik, dan toksikologi.
Arjuna Subject : Umum - Umum
Articles 94 Documents
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LITERATURE REVIEW : PERBANDINGAN EFEKTIVITAS PEMERIKSAAN KULTUR DAN POLYMERASE CHAIN REACTION (PCR) DALAM IDENTIFIKASI Neisseria gonorrhoeae PADA PASIEN GONORE Sahira Rauf
Jurnal Sains dan Teknologi Laboratorium Medik Vol 8 No 2 (2022): November (2022)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v8i2.102

Abstract

Gonorrhea is a disease caused by the bacterium Neisseria gonorrhoea that generally attacks the genital mucosa or cervical mucosa. Gonorrhea has a higher incidence than other infectious diseases. The spread of this disease is higher in developing countries including Indonesia. Culture examination is the gold standard for the diagnosis of gonorrhea, this examination has good sensitivity and very high specificity, while the weakness is that it takes a long time to get positive results. Polymerase Chain Reaction (PCR) is an in vitro technique that can amplify a specific DNA section that lies between two known DNA sections. PCR is a biomolecular technique used for the isolation and exponential amplification of target DNA fragments or sequences through enzymatic replication, or without the use of living organisms. The PCR technique is faster than culture, effective, accurate, efficient, high sensitivity and specificity. This study aims to determine the effectiveness and differences in the results of culture and Polymerase Chain Reaction (PCR) in identifying Neisseria gonorrhoea in gonorrhea patients. This study used a literature review conducted by collecting library data based on PICO keywords using Google Scholar and PubMed databases. The results of the statistical test for sensitivity were 0.001 (p<0.05) and statistical specificity test was 0.095 (p>0.05). There was a significant difference in terms of sensitivity and there was no significant difference in terms of the specificity of the results of culture and Polymerase Chain Reaction (PCR) examinations.
THE OVERVIEW OF HEMOGLOBIN LEVELS WITH CYANMETHEMOGLOBIN METHOD AND VOLUMETRIC IMPEDANCE IN PATIENTS EXPOSED TO MOSQUITO REPELLENT SPRAY Andy Setiawan Widodo
Jurnal Sains dan Teknologi Laboratorium Medik Vol 8 No 2 (2022): November (2022)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v8i2.107

Abstract

This study aims to determine differences in the results of hemoglobin levels of people exposed to mosquito repellent spray examined using Cyanmethemoglobin and Volumetric Impedance methods. This study employed a cross sectional design. The sample in this study amounted to 30 patients at Pandak 2 Primary Health Center Bantul. The data used secondary data obtained at Pandak 2 Primary Health Center Laboratory, Bantul. The data processing stages carried out in this study were editing, coding, and tabulating which would then the data was analysed using Independent sample T test. The Cyanmethemoglobin method showed that there were 11 patients with normal hemoglobin levels and the remaining 19 patients showed hemoglobin levels below normal, while the Volumetric Impedance method revealed 13 patients with normal hemoglobin levels and the remaining 17 patients showed hemoglobin levels below normal. Based on the results of the T sample Independent Test, there was no significant difference between the Cyanmethemoglobin and Volumetric Impedance methods in the examination of hemoglobin levels in patients exposed to mosquito repellent spray.
PENGARUH KONDISI PANDEMI TERHADAP KUALITAS UDARA AMBIEN (SO2 dan NO2) PADA TITIK KEMACETAN KOTA YOGYAKARTA Nur Hanifa Fajar Wulandari
Jurnal Sains dan Teknologi Laboratorium Medik Vol 8 No 2 (2022): November (2022)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v8i2.108

Abstract

Penelitian ini bertujuan untuk menganalisis perbandingan kualitas sulfur dioksida dan nitrogen dioksida pada kondisi sebelum terjadi pandemi, saat terjadi pandemi, dan pasca pandemi pada tahun 2019 hingga 2021. Lokasi penelitian di perempatan jalan raya Kota Yogyakarta. Pemeriksaan ini menggunakan metode pararosanilin dan Griess-Saltzman. Penelitian ini menggunakan rancangan deskriptif kuantitatif. Sampel pada penelitian ini berjumlah tujuh lokasi yang merupakan wilayah sampling Dinas Lingkungan Hidup Kota Yogyakarta. Data yang digunakan dalam penelitian ini menggunakan data sekunder yang didapatkan di Laboratorium Dinas Lingkungan Hidup Kota Yogyakarta. Tahap pengolahan data pada penelitian ini yaitu melakukan pengecekan data untuk memastikan data yang di perlukan sudah tersedia kemudian dilakukan analisis data menggunakan Uji One Way Anova. Hasil penelitian ini menunjukan konsentrasi sulfur dioksida masih di bawah batasan pencemaran udara. Hasil pengukuran nitrogen dioksida masih di bawah batasan pencemaran udara. Berdasarkan kategori ISPU pengukuran sulfur dioksida dan nitrogen dioksida menunjukan hasil dalam kategori baik. Berdasarkan hasil Uji One Way Anova terdapat perbedaan pengaruh di setiap tahunnya pada konsentrasi sulfur dioksida dan nitrogen dioksida.
IDENTIFIKASI Candida sp DALAM URIN IBU HAMIL DI KLINIK RAMLAH PARJIB 1 SAMARINDA Annisa Rahmawati
Jurnal Sains dan Teknologi Laboratorium Medik Vol 8 No 2 (2022): November (2022)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v8i2.110

Abstract

Based on the survey results, there are several pregnant women who experience symptoms of candidiasis vulvovaginal. All pregnant women who visited the Clinic Ramlah Parjib 1 Samarinda on 15 February 2022, admitted that they had never carried out laboratory tests such as examination of urine and vaginal secretions. This study aims to identify the Candida sp fungus in the urine of pregnant women at Clinic Ramlah Parjib 1 Samarinda. This type of research is descriptive and uses a consecutive sampling technique with a sample of 30 pregnant womens urine. Respondents in this study were pregnant women. Samples were obtained from the urine of pregnant women at Clinic Ramlah Parjib 1 Samarinda which met the inclusion and exclusion criteria, then microscopic tests. The results showed that 8 people had Candidiasis vulvovaginal , 26,7% were positive Candida sp and 22 people, 73,3% were negative Candida sp. In addition, the growth of contaminant but harmless fungi was found, namely Aspergillus sp as many as 6 samples with a percentage of 20%.
IDENTIFIKASI TELUR SOIL TRANSMITTED HELMINTHS (STH) PADA FESES ANAK-ANAK DENGAN METODE DIRECT SLIDE DAN PENGENDAPAN FORMALIN 10% Yeli Hartuti
Jurnal Sains dan Teknologi Laboratorium Medik Vol 8 No 2 (2022): November (2022)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v8i2.111

Abstract

Worm infection is one of the diseases that is still a health problem in children in developing countries, especially in children who have not been able to maintain personal hygiene.Worm infections can be caused by soil-transmitted worms that have been contaminated by the eggs or larvae of the Soil Transmitted Helminth worm. Identification is done by 2 methods, namely Direct slide method and 10% formalin deposition. This research is a type of non-experimental research with cross sectional design and sampling technique using purposive sampling. The sample used is the feces of children aged 5-11 years. The results of the study were 17 samples of feces that were examined, it was found that there were 6 samples infected with STH (Positive) while the other 11 samples were not infected with STH (Negative). Frequency Distribution of Mother's Knowledge about Worm Infections, good knowledge of 11.76%, 29.41% sufficient and 58.82% less.
ROSELLA (Hibiscus sabdariffa) SEBAGAI ALTERNATIF BAHAN PEWARNA HISTOLOGI Yeni Rahmawati; Saraswati Saraswati
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 1 (2023): April (2023)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v8i2.113

Abstract

ABSTRACT Hematoxylin-Eosin (HE) is a synthetic dye that is commonly used in histological examination. HE exposure in the long term has a negative impact on health. The development of alternative dyes is important. Therefore this literature study aims to determine the use of rosella (Hibiscus sabdariffa) in histological staining. Literature review was conducted from five data sources, namely Google Scholar, Medline, Oxford Academic, Research Gate and Science Direct. Based on a literature search, 10 journals were obtained which showed that rosella (Hibiscus sabdariffa) can be used as an alternative dye for various types of human or animal histological tissues. Extraction can be done on fresh or dry leaves and flower petals. Solvents that can be used in the extraction process are polar solvents, namely distilled water or alcohol. Extraction methods of soxhletation, reflux, maceration or boiling can be used in the extraction of rosella (Hibiscus sabdariffa). Staining can be done using pure or modified extracts. Various variations of extraction methods, types of solvents, concentrations and staining times, as well as the degree of acidity performed can give staining results that are as good as HE. Rosella (Hibiscus sabdariffa) can be used as an alternative to HE dye. Keywords: Rosella, Hematoxylin Eosin, staining, histology
Uji Daya Hambat Sari Bawang Putih (Allium Sativum) Terhadap Pertumbuhan Jamur Malassezia furfur Menggunakan Metode Difusi Kertas Cakram chelyn mareli sarungu
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 1 (2023): April (2023)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i1.120

Abstract

Malassezia furfur is a dimorphic fungus that is lipophilic and causes pityriasis versicolor. People who experience pityriasis versicolor usually treat with oral treatments such as ketocenazole, itraconazole, fluconazole and tropical treatments such as 2.5% sulfide shampoo. Treatment like this is not free from side effects if there is repeated infection and long-term use of drugs without a doctor's prescription so that it can cause resistance. Therefore, other alternative treatments use natural medicines. One of them is garlic (Allium Sativum). Garlic has the main active compound content is Allicin, then other active compounds are essential oils, alkaloids, tannins, saponins, and flavonoids. The purpose of the study was to determine the ability of garlic (Allium Sativum) at various concentrations of 70%, 75%, 80%, 85%, 90% against the growth of Malassezia furfur fungus. The research design is true experiment post test only with control. This study uses the agar diffusion method with paper discs. The results of this study are garlic juice (Allium Sativum) concentration of 80% has the ability to inhibit the growth of Malassezia furfur with an inhibition zone of 46.23 mm which has effectiveness with imidazole ketocenazole group drugs.
ANALISIS KADAR VITAMIN C PADA BAWANG MERAH (Allium cepa L.) JENIS BATU, SUMENEP, DAN TUTUG DENGAN METODE SPEKTROFOTOMETRI UV-VIS Dadang Muhammad Hasyim
Jurnal Sains dan Teknologi Laboratorium Medik Vol 8 No 2 (2022): November (2022)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v8i2.121

Abstract

Shallot (Allium cepa L.) is a vegetable spice that is widely used as a food seasoning to add flavor and enjoyment to cooking. Shallots are also widely used as a powerful traditional medicinal ingredient to treat various health problems. Shallots contain various kinds of nutrients and vitamins. One of the vitamins contained in shallots is vitamin C which acts as an antioxidant and is effective in dealing with free radicals that damage cells or tissues. This study aims to determine the levels of vitamin C in several types of shallots. The types of shallots in this study were stone shallots, sumenep shallots, and tutug shallots. Analysis of vitamin C levels was carried out using the UV-Vis spectrophotometry method at a wavelength of 515 nm. Results of the analysis showed that the vitamin C levels of stone shallots were 1.043 mg/100 g, Sumenep shallots were 1.638 mg/100 g, and tutug shallots were 1.296 mg/100 g. Vitamin C levels in Sumenep shallots have the highest levels compared to those of stone shallots and tutug shallots.
STUDI KASUS : GAMBARAN KARAKTERISTIK PENDERITA PENYAKIT INFEKSI MENULAR SEKSUAL AKIBAT BAKTERI NEISSERIA GONORRHOEAE DI PUSKESMAS MLATI 1 TAHUN 2022 Mezi Putri Arifani; Nadia Nanda Safitri; Nazula Rahma Shafriani; Diktia Sani Kisna Anita
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 1 (2023): April (2023)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i1.116

Abstract

Gonorrhea, also known as gonorrhea, is a contagious infectious disease caused by the bacterium Neisseria gonorrhoeae. This disease is transmitted through sexual intercourse and bad habits of changing partners. The symptoms are generally asymptomatic. Purpose: this study aims to discuss the Characteristicsof Sufferers of Sexually Transmitted Infection Disease Due to Neisseria gonorrhoeae bacteria based on age and gender at the Mlati 1 Public Health Center in January-December 2022. Method: the method used is descriptive with a Cross Sectional Study approach.Results: based on the research data conducted, the results of reactive gonorrhea were 21 cases (28%) with the largest population age being adolescents 15-25 years with a total of 11 cases (52.3%). Then, in the adult age group 26-45 years there were 8 (38.2%) cases and the elderly age group 46-65 years with 2 (9.5%) cases. Gonorrhea sufferers were predominantly found in males with a total of 18 cases (85.7%) and in females totaling 3 (14.3%). Conclusion: Overview of STI cases with reactive gonorrhea with a total of 21 cases occurring in the 15-25 year age group of adolescents, and found in males.
LITERATUR REVIEW: GAMBARAN PEMERIKSAAN KULTUR DARAH DAN POLYMERASE CHAIN REACTION REACTION PADA PASIEN DEMAM TIFOID Nurmi - Hasbi
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 1 (2023): April (2023)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i1.130

Abstract

Typhoid fever is a health problem in the world caused by the bacterium Salmonella typhi. The route of transmission of this disease is through oral-faeces. According to WHO it is estimated that every year around the world there are 10 to 21 million cases of typhoid fever. whereas in Indonesia in 2010-2019, the incidence rate is estimated to be around 500 per 100,000 population each year. The inability to make an early laboratory diagnosis often leads to increased morbidity and mortality in cases of typhoid fever. The S. typhi culture examination technique is the gold standard method for confirming the diagnosis of typhoid fever. Polymerase Chain Reacton (PCR) is an in vitro DNA synthesis and amplification technique. PCR technique can be used to amplify DNA segments of pathogenic bacteria millions of times in just a few hours. This study aims to determine the effectiveness of fever examination between culture and PCR techniques. This study used the literature review method which was carried out by collecting library data based on keywords, namely typhoid fever, culture, and PCR. Searching for articles through the Google Scholar and Pubmed databases. The results of the analysis of the literature review with a sensitivity statistical test were 0.001 (p<0.05) and the speficity test was 0.095 (p>0.05). There is a significant difference in terms of sensitivity and no significant difference in terms of specificity of blood culture and polymerase chain reaction (PCR) results

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