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Jurnal Sains dan Teknologi Laboratorium Medik
Published by Akademi Kesehatan John Paul II Pekanbaru
ISSN : 25275267     EISSN : 26217708     DOI : https://doi.org/10.52071/jstlm
Core Subject : Health, Science, Education,
Education Health Professions Immunology & microbiology Public Health Other
Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM, e-ISSN: 2621-7708, p-ISSN: 2527-5267) adalah sebuah jurnal yang didedikasikan untuk plublikasi hasil penelitian yang berkualitas dalam bidang sains dan laboratoium medik. semua publikasi di jurnal Jstlm bersifat akses terbuka yang memungkinkan artikel tersedia secara bebas online tanpa berlangganan apapun. Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM) dikelola secara profesional dalam hal membantu para akademisi, peneliti dan praktisi untuk menyebarkan hasil penelitiannya. focus dan scope Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM) adalah sains, mikrobiologi, parasitologi, hematologi, kimia klinik, dan toksikologi.
Arjuna Subject : Umum - Umum
Articles 94 Documents
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GAMBARAN TELUR CACING Oxyuris vermicularis PADA ANAK DI WILAYAH PESISIR PANTAI MERPATI BULUKUMBA Andi Harmawati Novriani. HS
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 1 (2023): April (2023)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i1.132

Abstract

Helminthiasis is a public health problem in Indonesia. Oxyuriasis is an infectious disease caused by Oxyuris vermicularis (Enterobius vermicularis) or pinworms and is a non-STH (non-Soil Transmitted Helminth) intestinal worm that can move from one individual to another without the need for transmission through the soil and is widespread in children and toddlers. Objective: This study aims to determine the picture of Enterobius vermicularis worm eggs in children aged 3-8 years in coastal areas. Methods: This research is descriptive using a purposive sampling technique. The population in this study was 20 samples. The sampling method used the anal swab method which is the best method in diagnosing Oxyuris vermicularis. Worm eggs are taken with an anal swab or cellophane swab attached around the anus in the morning before the child does activities such as defecating, urinating, and bathing. Results: out of 20 samples, 3 samples were infected with Enterobius vermicularis eggs and 17 samples were negative. Enterobiasis cases in coastal areas are still high. Conclusion: Based on the results of Enterobius vermicularis worm eggs research, found in children aged 3-8 years, out of 20 samples found 3 samples were infected with Enterobius vermicularis worm eggs and 17 samples were negative Enterobius worm eggs. Where Enterobius vermicularis cases are still high in coastal areas.
PENGARUH WAKTU PENUNDAAN PEMERIKSAAN TERHADAP KADAR KOLESTEROL Prima Octafia Damhuri
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 1 (2023): April (2023)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i1.133

Abstract

Pemeriksaan laboratorium merupakan pemeriksaan penunjuang yang dapat digunakan dalam menegakkan diagnosa suatu penyakit, oleh sebab itu hasil pemeriksaan laboratorium harus akurat dan dapat dipercaya. Seringkali pemeriksaan tidak dapat dilakukan dengan segera sehingga terjadi penundaan waktu pemeriksaan yang bervariasi. Hal ini perlu mendapat perhatian mengingat banyaknya faktor yang dapat mempengaruhi kecepatan dan ketepatan hasil pemeriksaan. Pemeriksaan kolesterol total dianalisis karena pemeriksaan tersebut merupakan salah satu pemeriksaan yang paling sering dilakuakan di laboratorium. Pemeriksaan kadar kolesterol tidak dapat segera dilakukan atau terpaksa ditunda apabila terjadi kerusakan pada alat di laboratorium, sehingga sampel harus disimpan sebelum analisis dilakukan. Beberapa faktor yang mempengaruhi peningkatan kadar kolesterol yaitu penundaan pemeriksaan, ketidak seimbangan komposisi enzim yang ada dalam serum yang akan diperiksa. Tujuan dari penelitian ini adalh untuk melihat pengeruh waktu penundaan pemeriksaan terhadap kadar kolesterol. Metode pengambilan sampel yang digunakan adalah random sampling yaitu sebanyak 9 sampel. Dari hasil penelitian yang telah dilakukan diperoleh hasil rerata kadar kolesterol yang diperiksa segera 167,11 mg/dL, yang ditunda selama 2 jam sebesar 153,22 mg/dL dan yang diperiksa setelah 4 jam adalah 131,33 mg/dL. Uji statistik menunjukkan Terdapat perbedaan yang signifikan hasil kadar kolesterol terhadap penundaan waktu pemeriksaan baik 2 jam maupun 4 jam dengan nilap p 0,002 (<0,05).
UJI CEMARAN Salmonella sp PADA MAKANAN JAJANAN SEKOLAH DASAR DI KELURAHAN TOBEK GODANG KECAMATAN TAMPAN PEKANBARU Siska Zafrida
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 1 (2023): April (2023)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i1.135

Abstract

Makanan merupakan kebutuhan pokok bagi manusia agar bisa hidup sehat dan dapat melakukan aktifitas sehari-sehari dengan baik. Namun dari berbagai macam makanan yang dikonsumsi oleh manusia juga terdapat makanan yang menyebabkan penyakit pada manusia. Hal ini terjadi dikarenakan dalam pembuatan makanan yang dikonsumsi kurang higienis.Penelitian ini bersifat Eksperimental laboratory dengan cara kultur. Penelitian ini bertujuan untuk mengetahui ada tidaknya cemaran Salmonella sp pada jajanan makanan yang dijual dikantin SDN 165 dan SDN 176 Kelurahan Tobek Godang Kecamatan Tampan Pekanbaru. Dari 8 sampel jajanan makanan yang dibiakan pada media Selenit broth terjadi kekeruhan yang menandakan adanya pertumbuhan bakteri dan setelah dilakukan pewarnaan Gram ditemukan basil Gram negatif pada 8 sampel jajanan makanan. Hasil pertumbuhan koloni pada media kultur SS agar setelah dibiakan 37°C selama 1 x 24 jam pada 8 sampel ditemukan pertumbuhan koloni dengan ciri-ciri koloni bulat, putih. Berdasarkan hasil reaksi biokimia dapat disimpulkan bahwa sampel jajanan makanan di kantin SDN 165 dan SDN 176 Kelurahan Tobek Godang Kecamatan Tampan Pekanbaru tidak ditemukan Salmonella sp dengan persentasi cemaran sebesar 0%.
IDENTIFIKASI TELUR CACING PADA KUKU ANAK-ANAK DI TPA MUARA FAJAR KOTA PEKANBARU Weni Mulyani
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 1 (2023): April (2023)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i1.136

Abstract

Kecacingan merupakan salah satu penyakit yang masih menjadi masalah kesehatan bagi masyarakat Indonesia hingga saat ini. Penyakit infeksi kecacingan bisa di tularkan melalui kaki, tangan atau kuku yang terkontaminasi tanah yang mengandung telur cacing, lalu masuk ke mulut bersama makanan. penyakit infeksi kecacingan yang menyerang usus terdiri atas 3 phylum yaitu nematoda, cestoda dan trematoda. Salah satu daerah di Pekanbaru yang rentan terhadap kecacingan adalah Tempat Pembuangan Akhir (TPA) Muara Fajar Kota Pekanbaru, wilayah ini sangat lembab dan kumuh. TPA Muara Fajar selain menjadi tempat pembuangan sampah di Kota Pekanbaru ternyata juga merupakan kawasan sumber aktivitas ekonomi bagi para pemulung. Tidak hanya pemulung usia dewasa, tetapi juga pemulung usia anak-anak. Anak-anak yang tinggal disekitar daerah ini memiliki risiko lebih tinggi terinfeksi kecacingan, karena TPA dijadikan sebagai tempat bermain dan bekerja bagi anak-anak yang tinggal di wilayah tersebut. Penelitian ini bertujuan untuk mengidentifikasi telur cacing pada kuku tangan dan kuku kaki anak-anak di TPA Muara Fajar Kota Pekanbaru. Hasil penelitian ditemukan telur cacing Ascaris lumbricoides pada kuku tangan sebanyak 4 sampel, pada kuku kaki 4 sampel dan telur Taenia sp pada kuku tangan sebanyak 2 sampel, kuku kaki 1 sampel. Berdasarkan penelitian disimpulkan bahwa terdapat 2 jenis telur cacing yang ditemukan pada kuku tangan dan kuku kaki anak-anak di TPA Muara Fajar Pekanbaru yaitu telur cacing Ascaris lumbricoides dan Taenia sp.
Profil Hitung Jumlah dan Jenis Leukosit Pada Anak Terinfeksi Kecacingan Kota Samarinda Ade Tasya Halim; Sresta Azahra
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 2 (2023): November 2023
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i2.137

Abstract

Helminthic infections are caused by helminth parasites. Helminthic worms are health problems that affect the defenses of the human immune system such as leukocytes. Children infected with helminthic worms are characterized by an increase in the number of eosinophils due to an immune response to helminthic infection. The purpose of knowing the profile of counting the number and type of leukocytes in children infected with worms. Type of descriptive research with cross sectional design. The total population is 187 children with 9 children infected with worms in Samarinda City. This study was carried out by fecal examination (microscopic), leukocyte count examination (counting chamber) and leukocyte count (differential count). Analysis of data in univariate form. The results of the study found 9 children (5%) infected with worms, 5 children (3%) male aged 6-12 years and 4 children (2%) female aged 8-10 years. Types of worm eggs obtained 5 children (56%) Ascaris lumbricoides, 2 children (22%) Trichuris trichiura and 2 children (22%) Hymenolepis nana. The number of leukocytes in infected children is within normal limits (4,500-13,500 μL). The type of abnormal leukocytes was obtained by 4 children (45%) who had elevated eosinophils. Conclusion The number of leukocytes in infected children is within normal limits and the type of leukocytes that have increased in the number of eosinophils (45%) indicates an immune response to helminthic infection.
Analisis Kadar Vitamin C Pada Jus Buah Nanas Dengan Penambahan Pemanis Alami Dan Pemanis Buatan Berdasarkan Lama Waktu Penyimpanan Linda Sari Dwi Setyaningsih; Eka Farpina; Ganea Qorry Aina
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 2 (2023): November 2023
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i2.141

Abstract

Vitamin C is one of the main vitamins needed by humans every day. Pineapple fruit contains vitamin C which can be obtained through direct consumption or consumed after processing, such as into juice. Vitamin C in fruit juice may decrease with the storage time, but the decrease in vitamin C levels can be suppressed by adding sweeteners. The purpose of this study was to determine the presence and absence of differences in vitamin C levels in pineapple juice with the addition of natural sweeteners and artificial sweeteners based on the length of storage time. The type of research used is purely experimental. The study population was all pineapples harvested from pineapple plantations, Tani Bakti Village, Samboja District, Kutai Kartanegara Regency. The sample was pineapple juice made from Queen variety pineapple fruit with 4 variations of 10% concentration sweetener: sucrose, saccharin, cyclamate, and aspartame, and 1 variation of unsweetened control. With 4 variations of storage time namely 0, 30, 60, and 120 minutes. Analysis of vitamin C levels is carried out using the iodimetric titration method. The results of the study found natural sweeteners and artificial sweeteners can suppress the decrease in vitamin C levels in pineapple juice when stored. The data from the study was then carried out static tests and obtained a value of p≤0.05 which showed that there was a difference in vitamin C levels in pineapple juice with the addition of natural sweeteners sucrose and artificial sweeteners saccharin, cyclamate, and aspartame during the storage period. After further static tests were carried out and it was found that based on all storage times there was a significant difference between sucrose-sweetened fruit juice, against control fruit juice with a p-value of ≤0.05. While from pineapple fruit juice other sweeteners did not show any significant difference from control pineapple fruit juice (unsweetened).
OBSERVASI KADAR SGOT DAN SGPT PADA PEROKOK AKTIF BERDASARKAN USIA DEWASA DI WILAYAH BUKUAN RT 13 Annisa Putri Yasmeen
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 2 (2023): November 2023
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i2.142

Abstract

Smoking is a common bad habit worldwide and has the potential to cause a variety of serious diseases, including liver disorders. The enzymes SGOT and SGPT are used as sensitive indicators to measure liver function. This study aims to determine the levels of SGOT and SGPT in adult active smokers in the Bukuan RT 13 area. This study used descriptive method and cross sectional research design. The study population consisted of 20 adult active smokers in Bukuan RT 13 with total sampling technique. Data obtained from the results of the examination of SGOT / SGPT levels and interviews then analyzed univariate. The results showed that of the 20 respondents, all (100%) were active smokers with a smoking duration of more than 5 years. Abnormal SGOT levels were found in 12 people (60%), while SGPT was normal in 18 respondents (90%). The group who smoked 11-20 cigarettes per day had 5 respondents (25%) with normal SGOT results, while the group who smoked more than 20 cigarettes per day had 8 respondents (40%) with abnormal SGOT levels. Normal SGPT levels were found in 10 respondents (50%) from the group who smoked more than 20 cigarettes per day. The conclusion of this study is that some respondents showed abnormal SGOT and SGPT levels, which indicates the need for attention in the prevention and management of smoking-related health problems in active smokers with a smoking duration of more than 5 years.
ANALISIS KADAR UREUM DAN KADAR GLUKOSA DARAH PUASA PADA PEROKOK KONVENSIONAL DAN PEROKOK VAPE USIA PRODUKTIF 18-21 TAHUN Nazla Filda Apriliani
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 2 (2023): November 2023
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i2.144

Abstract

Conventional cigarettes and vape cigarettes contain nicotine. Nicotine in cigarettes has an influence on urea levels and blood glucose levels. The aim of this research is to determine urea levels and fasting blood glucose levels in conventional smokers and vape smokers of productive age 18-21 years. This type of research is descriptive and the variable in this research is 18-21 years old. The sampling technique uses purposive sampling technique. Samples were taken by taking venous blood which was then checked for urea levels and fasting blood glucose levels using a spectrophotometer. The data analysis used in this research is univariate analysis.The research results obtained: the average urea level of conventional smokers was 20.07 mg/dL and that of vape smokers was 20.85 mg/dL, the fasting blood glucose level of conventional smokers was 81.18 mg/dL and that of vape smokers was 77.04 mg/dL. The percentage of urea levels for conventional smokers is 93% normal, 7% is not normal, while vape smokers are 100% normal, not normal, 0%. The percentage of fasting blood glucose levels for conventional smokers is 60% normal, 40% abnormal, while for vape smokers it is 47% normal and 53% abnormal. Based on these results, both the average and percentage of urea levels and fasting blood glucose levels in conventional smokers and vape smokers of productive age 18-21 years are still within normal limits. Keywords: Conventional cigarettes, Fasting blood glucose, Urea, Vape cigarettes
GAMBARAN KADAR SGOT DAN SGPT PADA PETANI YANG MENGGUNAKAN PESTISIDA DI DESA LOA JANAN ULU Handa Nofianti Rosadi
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 2 (2023): November 2023
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRACT Indonesia is an agricultural country where the agricultural sector is one of the main sources of the economy. However, farmers are worried about pest attacks, so they choose to use pesticides in order to increase agricultural yields. Therefore, in Indonesia, many farmers use pesticides, especially in East Kalimantan, precisely in the village of Loa Janan Ulu. The use of pesticides that violate the rules can endanger health, one of which is liver damage. Examination of SGOT (Serum Glutamic Oxoloacetic Transminase) and SGPT (Serum Glutamic Pyruvic Transminase) levels is one of the markers of liver damage, therefore this study aims to determine the average levels of SGOT and SGPT and the percentage of SGOT and SGPT levels that exceed the normal rate in farmers who use pesticides. The type of research used is descriptive with purposive sampling technique where the number of samples is 37 people. Based on the results, the highest average SGOT levels are at the age of 30-50 years (56 U/L) and work duration >10 years (55 U/L). While the highest average SGPT levels are aged 30-50 years (40 U/L) and have worked >10 years (39 U/L) and the percentage of SGOT levels is 95% and SGPT levels are 46% which exceeds the normal rate. It can be concluded that the average levels of SGOT and SGPT are highest at the age of 30-50 years and have worked for >10 years with a higher percentage of SGOT levels that exceed the normal rate than SGPT. Keywords: Fermers, Pesticide, SGOT, SGPT
ANALISIS KADAR ENZIM CHOLINESTERASE PADA PETANI YANG MENGGUNAKAN PESTISIDA DI DESA LOA JANAN ULU Niken Syafitri
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 2 (2023): November 2023
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i2.146

Abstract

In Indonesia, many farmers use pesticides, especially in East Kalimantan, specifically in Loa Janan Ulu Village. Farmers use pesticides as an effort to reduce pest or insect attacks. The use of pesticides poses a risk to humans in the form of acute chronic poisoning and even death. Cholinesterase is an enzyme found in cell fluids whose job is to stop the action of acetylcholine by hydrolyzing it into choline and acetic acid. The aim of this research was to determine the levels of the cholinesterase enzyme in farmers who use pesticides in Loa Janan Ulu village. The type of research used was descriptive with a sample of 80 farmers in Loa Janan Ulu village who met the inclusion and exclusion criteria. The sampling technique was carried out using purposive sampling, with univariate analysis. The research results are based on the characteristics of respondents in the largest age group, namely 51-60 years (15 respondents), working period 21-30 years (14 respondents). The frequency of spraying was once a week (25 respondents). It can be concluded that in 33 samples the cholinesterase enzyme levels of 95% of farmers met normal standards and 5% did not meet normal standards.

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