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Hanif Amrulloh
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Journal of Multidisciplinary Applied Natural Science
Published by Pandawa Institute
ISSN : -     EISSN : 27743047     DOI : 10.47352/jmans
Core Subject : Science, Education, Agriculture, Social, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics
Journal of Multidisciplinary Applied Natural Science (abbreviated as J. Multidiscip. Appl. Nat. Sci.) is a double-blind peer-reviewed journal for multidisciplinary research activity on natural sciences and their application on daily life. This journal aims to make significant contributions to applied research and knowledge across the globe through the publication of original, high-quality research articles in the following fields: 1) biology and environmental science 2) chemistry and material sciences 3) physical sciences and 4) mathematical sciences. The J. Multidiscip. Appl. Nat. Sci. is an open-access journal containing original research articles, review articles, and short communications in the areas related to applied natural science. The J. Multidiscip. Appl. Nat. Sci. publishes 2 issues in a year on January (first issue) and July (second issue). This journal has adopted a double-blind reviewing policy whereby both the referees and author(s) remain anonymous throughout the process.
Arjuna Subject : Umum - Umum
Articles 25 Documents
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Search results for , issue "Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science" : 25 Documents clear
Potency of Cocoa Honey, Byproduct of Cocoa Bean Processing as Probiotic Media: Optimization of Saccharomyces boulardii Growth and Its Antimicrobial Activity Neti Yuliana; Fibra Nurainy; Sumardi Sumardi; Zikrina Marentina; Nabila Rizka Putri
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.219

Abstract

Cocoa honey, a byproduct of cocoa bean processing, contains high sugars and minerals; thus, it has potential as a fermentation medium. This research investigated the potency of cocoa honey as probiotic media for promising yeast probiotic Saccharomyces boulardii. The growth of S. boulardii in the cocoa honey medium was optimized through response surface methodology employing a central composite rotatable design to obtain maximum cell production and antimicrobial activity. The effects of inoculum size (2–5%), sucrose addition (2.0–3.0%), and fermentation time (5–20 h) were evaluated. The agar diffusion technique assessed the cocoa honey medium's antibacterial activity. Results showed that these independent variables significantly affected the production of S. boulardii cells and their microbial activity against Escherichia coli. The optimal values generated by the model with a desirability value of 0.908 were 20 h of fermentation time, 2% of sucrose addition, and 2% of inoculum size. The maximum yeast count (log10 8.07 cells/mL) and antibacterial activity (4.88 mm) were reached under these optimized conditions, which agreed with the predicted value of the RSM model. This study provided valuable information on the potency of cocoa honey as a fermentative medium for applying S boulardii to develop a probiotic drink.
Detection of csg and lux Genes in Biofilm-Forming Uropathogenic Escherichia coli Associated with Urinary Tract Infections Rini Purbowati; Sri Lestari Utami; Dadik Raharjo; Masfufatun Masfufatun
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.222

Abstract

Uropathogenic Escherichia coli (UPEC) is responsible for 80–90% of urinary tract infections (UTI) in the global population. The emergence of the increasing resistance to broad-spectrum antimicrobial agents was due to the ability to form biofilms. Cell surface factors that play a role in biofilm formation include Quorum Sensing (QS) which is encoded by the luxS family gene and curli by two operons, namely the csgBA operon. The purpose of the study is to detect the effects of 2 virulence genes (csgD and luxS) on biofilm-forming UPEC associated with UTI. As many as 76 UPEC isolates were collected from the clinical microbiology laboratories and the biofilm development was analyzed using the crystal violet method on microplate 96 wells. Using PCR assay, the two studied genes (csgD and luxS) were determined to be present in the isolates. UPEC isolates the bacteria-produced biofilms (90.80%) and nonproducers (9.20%). Most UPEC bacteria (97.36%) are known to be positive for csgD and luxS gene, while the others (92.10%) are known to be positive for the luxS gene. The highest proportion of the genes expressed in this study is followed by the presence of a relationship between the ability to produce biofilm and the presence of the genes under investigation, which is followed by all UPEC strains that cause UTI in humans.
Potential of Eco-enzyme Averrhoa bilimbi L. Fruit as an Innovation in Tempeh Wastewater Treatment in Plaju District, South Sumatra Siti Soleha; Delima Engga Maretha; Andi Saputra
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.224

Abstract

Tempeh home industries have been established in residential areas, one of which is in the assisted village of PT Pertamina Internasional RU III Plaju Factory, Palembang, South Sumatra. Eco-enzyme is one of the methods for processing and reducing pollutant organic matter in liquid waste. This study aims to determine the eco-enzyme characteristics of Averrhoa bilimbi L. fruit and exploit its potential to reduce chemical oxygen demand (COD), total suspended solid (TSS), and total dissolved solid (TDS) in tempeh wastewater. Eco-enzymes are characterized by measuring pH, COD, TSS, TDS, and most probable number (MPN). The reduction in COD, TSS and TDS of tempeh wastewater was observed using a simple bioreactor which was treated with the addition of 10% eco-enzyme (three replicates) and without the addition of eco-enzyme (control). The characterization results showed that the eco-enzyme of A. bilimbi L. fruit had a pH of 1.84, COD of 85.33 mg/L, TSS of 440 mg/L, TDS of 15,800 mg/L, protein concentration of 0.459 U/mg, and MPN value of 0.03 CFU/100 mL. The COD value of tempeh liquid waste can be reduced from the initial COD of 256 to 154.67 mg/L. The TSS value of tempeh wastewater decreased by 60% from the initial value of 4,000 mg/L. It takes 18 days to reduce the TSS content below the quality standard. The TDS value of tempeh liquid waste can be reduced by 70% from the initial TDS of 7,333.33 to 1,666.67 mg/L.
The Effect of a Combination of Ethanol Extract from Leaves and Flowers of Plumeria acuminate L. Against Aedes aegypti Larvae Martha Indah Widiyaningtiyas; Rizky Ardinansyah Putra; Evy Ratnasari Ekawati; Dheasy Herawati; Arifa Mustika
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.225

Abstract

Aedes aegypti is the primary vector that causes dengue virus (DENV) infection. Ae. aegypti resistance to abate (Temephos) has occurred in Indonesia and several other countries. Innovations are needed to develop alternative ingredients that can be used as natural larvacides. The Plumeria acuminate L. is an Indonesian plant with metabolites that have the potential to act as larvicides. This study aims to determine the potential combination of ethanol extract of P. acuminate L. leaves and flowers as a larvicide for A. aegypti. A laboratory experimental study with a post-test-only control group design was carried out on Ae. aegypti larvae instar III. The larvicide test was carried out according to WHO standards in 2005 with concentrations of 10000, 7500, 5000, and 2500 ppm, as well as control (water and 1% DMSO), with 20 larvae for each concentration. The observation was carried out at the 24th and 48th hours with 3 repetitions. Larval mortality data was analyzed using one-way ANOVA statistical tests to determine significant differences and lethal concentrations (LC50 and LC90) were calculated using probit analysis using SPSS software. The mortality of Ae. aegypti larvae due to the administration of a combined ethanolic extract of P. acuminate L. leaves and flowers was the highest at doses of 10000 and 7500 ppm, which was 100% with lethal concentrations of LC50 and LC90 of 3364.715 and 6293.759 ppm at the 24th hour observation, whereas at the 48th-hour observation, lethal concentrations were detected at 1767.998 and 2941.138 ppm, respectively. One-way ANOVA analysis test showed a significant difference in Ae. aegypti larval mortality due to the administration of a combined ethanol extract of P. acuminate L. leaves and flowers at 24th-hour observation (p = 0.000) and (p = 0.013) at 48th hour observation. The combination of ethanol extract of P. acuminate L. leaves and flowers showed larvicidal activity against Ae. aegypti larvae as evidenced by larvae mortality which were influenced by concentration and observation time.
Investigation of the Anti-diabetic and Anti-lipid Peroxidative Properties of Gallic Acid in Diabetic Rats Mitra Arman; Mostafa Alinaghizadeh; Mahmoud Kordi; Saeid Tamadoni Jahromi; Hanif Amrulloh; Hamed Kioumarsi
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.226

Abstract

Diabetes mellitus is a chronic non-communicable disease that occurs due to insulin deficiency or reduced function in the body. Therefore, the scientific investigation of herbal plants and their effectiveness is essential. It has been shown that gallic acid has hypoglycemic properties. In this study, the anti-diabetic and anti-lipid peroxidation properties of gallic acid were investigated. For this purpose, Streptozotocin was injected intraperitoneally into male rats at a dose of 60 mg/kg to induce type I diabetes. Seven days after Streptozotocin injection, blood samples were taken and rats with serum glucose levels higher than 250 mg/dL were considered diabetic. The rats were divided into 4 groups. Two groups of diabetic rats received either water or gallic acid at a dose of 40 mg/kg, and two groups of normal rats received either water or gallic acid at 40 mg/kg dose. The treatment period for all groups was 8 weeks. After the end of the period, blood samples were taken from the rats under fasting conditions. The collected blood samples were analyzed for serum biochemical factors (glucose, cholesterol, triglycerides, High-density lipoprotein, and creatinine) using spectrophotometry with the respective kits, and the level of lipid peroxidation in red blood cells was measured. The administration of gallic acid at a dose of 40 mg/kg (8 weeks) significantly reduced serum levels of glucose, triglycerides, cholesterol, alanine aminotransferase, aspartate aminotransferase, very low-density lipoprotein, low-density lipoprotein, and creatinine, while significantly increasing serum levels of high-density lipoprotein. Additionally, the level of Malondialdehyde in red blood cells was decreased in the diabetic group receiving gallic acid compared to the diabetic control group, and in normal rats receiving gallic acid at a dose of 40 mg/kg (8 weeks), it had no significant effect on serum factors and had no toxicity.
Characterization of the Small Robotic Telescope Instrument and Implementation at ITERA Lampung Astronomical Observatory Hakim L Malasan; Robiatul Muztaba; Aditya Abdillah Yusuf; Adhitya Oktaviandra; Mitra Djamal; Tulin Bedel; Peter Aniol
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.227

Abstract

As time has passed, the technological advances used to run these telescope systems have become faster, more reliable, and more user-friendly. Institut Teknologi Sumatera (ITERA) has installed a small robotic telescope at the Meteorology–Climatology–Geophysics Observing Station in collaboration with an ASTELCO System. The telescope system is designed to make fully robotic observations and can operate in both interactive and unattended robotic modes. This study's primary focus is on the OZT-ALTS telescope robotic system with the optical system using triplet apochromatic lens technology manufactured by LZOS with a focal ratio of f/8 refractor design. The telescope mount is a German Equatorial (NTM-500 manufactured by ASTELCO) with a direct drive system with an absolute encoder, and it allows fully programmable tracking speeds with a typical slewing speed of 20°/sec and a tracking accuracy range of 0.3–1.0 arcsec. For the process of collecting moon observation images, we use Manta G-031B. The OZT-ALTS robotic telescope is now contributing to monitoring crescent moon observation and astronomical studies. This paper presents the scientific motivation behind the OZT-ALTS robotic telescope and the facility's specifications and unique features. Furthermore, we present to characterize the result of the hardware OZT-ALTS robotic telescope system to increase the mechanical and optical performance of the telescope system based on the in-situ test observations. Based on the result, setting the Gain on the camera at 3.5 e-/ADU is the limit for the CCD to produce good-quality output images by utilizing the entire dynamic range without saturation. We also present an example of the science we have performed with the OZT-ALTS robotic telescope. The OZT-ALTS can observe young crescent moon events in the daylight after implementing the CLAHE technique to enhance contrast objects. On the other hand, the OZT-ALTS can also observe WASP-16b and WASP-34b exoplanets by using the transit method to analyze the decreasing light curve when a planet passes between a star and its observer.
Integrating The Network Pharmacology and Molecular Docking Confirmed with In Vitro Toxicity to Reveal Potential Mechanism of Non–Polar Fraction of Cyperus rotundus Linn as Anti-Cancer Candidate Susianti Susianti; Syaiful Bahri; Sutopo Hadi; Arif Setiawansyah; Lanang Rachmadi; Ibnu Fadilah; Maya Ganda Ratna
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.228

Abstract

Cyperus rotundus Linn is a plant that is historically used in traditional medicine with anti-cancer potential. Despite the evidence of C. rotundus anti-cancer effect on various human carcinoma cell lines, its pharmacological mechanism remains unclear, particularly its non-polar fraction. This study was employed to provide mechanistic insight regarding the anti-cancer properties of C. rotundus non-polar fraction by integrating in silico and in vitro approach. The network pharmacology study was used to observe the molecular targets of n-hexane fraction of C. rotundus, confirmed by molecular docking simulation using Autodock 4.2. The in vitro toxicity using BSLT method was used to strengthen the in silico result. The network pharmacology investigation revealed several core targets including PI3K, MAPK1, mTOR, RAF1, and NF-κB in the potential anti-cancer mechanism of C. rotundus. The molecular docking study illustrated that compound 3 (Isopetasol) and compound 9 (alpha-cyperone) as the most promising compound in n-hexane fraction of C. rotundus, with free binding energies consistently less than -7 kcal/mol in all targets. The in vitro BSLT signified the in silico results, highlighting the highest toxicity of fraction 3 exhibited among others. Integrating the network pharmacology and molecular docking simulation along with in vitro toxicity have provided evidence of the anti-cancer potential of n-hexane fraction of C. rotundus. Specific compounds and the molecular targets responsible for its anti-cancer properties have been identified, warranting further investigations.
Effect of The Water Origin on The Biological Properties of Sage (Salvia officinalis L.) Aqueous Extracts Irina Korotkova; Tamila Romashko; Oleg Khakhel'; Tamila Zvenihorodska; Tetiana S Yaprynets; Viktor Liashenko
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.230

Abstract

Plant extracts, due to the high content of various biologically active compounds, have found their wide application in the foods, pharmaceutical industries, and agricultures, for the production of valuable and useful bioproducts. The aqueous plant extracts are most often used. The effect of the water origin used in their preparation on the extract’s composition has not been studied enough, which limits their use. The study was aimed to compare the biological effect of sage (Salvia officinalis L.) extracts prepared using water of different origins (distilled, tap, spring, bottled). The preparation of aqueous extracts was carried out in two ways, differing in the extraction temperature. In the first method, boiling water was used to prepare the extract; in the second, cooled boiled water was used. The sage leaves mass for extract preparation was 1.65 and 3.30 g. The biological activity of the extracts was assessed by testing them for radish germination. It was shown the sage leaves extracts prepared using various waters origin can differ by one and half times in their allelopathic effect on radish seeds. It was proved the qualitative composition and mass of the substance extracted from the plant depend on the water origin used for extracts preparation. Thus, using the same amount of plant material to prepare extracts, you can increase their biological activity by fifty percent with the right choice of water.
Evaluation of Xanthone and Cinnamoylbenzene as Anticancer Agents for Breast Cancer Cell Lines through In Vitro and In Silico Assays Yehezkiel Steven Kurniawan; Hanif Amrulloh; Ervan Yudha; Nela Fatmasari; Faris Hermawan; Anggit Fitria; Harno Dwi Pranowo; Eti Nurwening Sholikhah; Jumina Jumina
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.231

Abstract

Breast cancer is a severe global disease for women as the number of deaths increases annually. Therefore, attempts to find new anticancer agents are critical and inevitable. In this work, we report the investigation on the anticancer activity of xanthone and cinnamoylbenzene compounds against two breast cancer cell lines, i.e., T47D and MCF-7, through experimental in vitro and theoretical in silico assays. Xanthone and cinnamoylbenzene exhibit anticancer activity with a half-maximal inhibitory concentration (IC50) of 136.7–194.3 and 235.8–262.4 µg/mL against T47D and MCF-7 cancer cells, respectively. Cinnamoylbenzene generates less cytotoxicity to normal Vero cells with a selectivity index of 1.095–2.102. The molecular docking studies agree with the experimental data in which cinnamoylbenzene is more active against T47D with an IC50 of 136.7 µg/mL due to Topoisomerase II inhibition through π-π stacked interactions with Adenine12 and Guanine13 nitrogen bases. Meanwhile, xanthone is more active against MCF-7 with an IC50 of 235.8 µg/mL due to EGFR inhibition through van der Waals interaction and hydrogen bond with Glutamic acid767 and Methionine769 amino acid residues, respectively. Additionally, the pharmacokinetic parameters of xanthone and cinnamoylbenzene are predicted through absorption, distribution, metabolism, excretion, and toxicity (ADMET) analysis, and they show better suitability than doxorubicin as the commercial anticancer drug.
Residential Environment and Parental Lifestyle as Risk Factors of The Co-Occurrence of Cleft Lip and Palate Birth Defect Cases in Indonesia Katharina Oginawati; Intan Nur’azizah Rahman; Nurul Fahimah; Rinaldy Jose Nathanael; Imtiyaz Olaf Jatmy; Taqya Izdihar Muhanandi; Ida Latifah; Andi Tajrin; Ikeu Tanziha; Shinta Silvia; Farah Asnely Putri; Ida Ayu Astuti; Asri Arumsari; Utriweni Mukhaiyar
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.232

Abstract

Cleft lip and palate (CLP) are multifactorial birth defects, and the etiology behind their occurrence remains insufficiently understood. This case-control study aimed to investigate the association between residential environmental factors, parental lifestyle, and the risk of CLP in Indonesia. A total of 213 participants from 14 provinces were selected using stratified random sampling. Multivariable logistic regression was employed to determine the odds ratios (ORs) with 95% confidence intervals (CIs) for each risk factor. The study identified significant associations between several residential environmental factors and CLP. Proper kitchen ventilation was found to be protective (OR = 0.245, 95% CI: 0.096–0.624, p = 0.003), while proximity to industrial sites significantly increased the risk (OR = 3.595, 95% CI: 1.029–12.558, p = 0.045). Additionally, using treated drinking water showed a protective effect (OR = 0.39, 95% CI: 0.16–0.95, p = 0.038), whereas concerns about water sources displayed a marginally significant risk increase (OR = 2.841, 95% CI: 0.876–9.211, p = 0.082). Regarding parental lifestyle factors, maternal active smoking exhibited a marginal protective association (OR = 0.052, 95% CI: 0.002–1.09, p = 0.057), while paternal passive smoking presented a marginally significant risk (OR = 2.309, 95% CI: 0.897–5.942, p = 0.083). Furthermore, paternal radiation exposure was associated with an elevated risk of CLP (OR = 3.489, 95% CI: 0.828–14.703, p = 0.089). These findings highlight the need for targeted public health interventions to mitigate environmental exposures and promote safer parental lifestyle behaviors in Indonesia.

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