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Journal of Natural Sciences
Published by Mahesa Research Center
ISSN : -     EISSN : 27215571     DOI : 10.34007
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Natural Sciences is a scientific publication media in the study and development of Science in the fields of science and Education (Biology, Chemistry, and Physics). The Journal of Natural Sciences includes scientific writings in the form of conceptual, research results as well as relevant issues in the fields of science and education. The Journal of Natural Sciences is published three times a year.
Arjuna Subject : Ilmu Lingkungan (semua ketegori) - Ilmu Lingkungan (Lain-Lain)
Articles 117 Documents
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Pengembangan Instrumen Tes Multiple Choice Reasoning Terbuka Untuk Mengukur Keterampilan Berpikir Kritis Siswa Dini Khoirunnisa Sofyan; Sabani Sabani
Journal of Natural Sciences Vol 3, No 3 (2022): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v3i3.297

Abstract

This study aims to develop a HOTS-based test instrument in measuring students' critical thinking skills on the matter of effort and energy. This research is a development research or Research and Development using the ADDIE model, namely Analysis, Design, Development, Implementation and evaluation. The subjects in this study were students of class X SMAS Gema Buwana. In the content validation test by the validator the question is classified as a valid instrument. While the validity of the items obtained 9 questions are said to be valid or feasible. The reliability of the test on the questions belongs to the reliable category with a very high reliability interpretation with a result of 0.879. To test the level of difficulty of the questions obtained 1 question with easy criteria, 2 questions with difficult criteria and 6 questions with moderate criteria. While the differentiating power of the questions obtained 3 questions with improved criteria and 6 questions with accepted criteria. The effectiveness of the distractor results in 5 answer choices with rejected criteria and 31 answer choices with accepted criteria. Based on the results of the development carried out, it can be concluded that the HOTS-based open multiple choice answering test instrument is said to be feasible to measure students' critical thinking skills.
Kemampuan Bakteri Kitinolitik Dalam Menghambat Pertumbuhan Fusarium oxysporum Rahmiati Rahmiati; Nikmah Ridha Batubara
Journal of Natural Sciences Vol 3, No 3 (2022): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v3i3.305

Abstract

Chitinolytic bacteria have the ability to produce chitinase enzymes, so they have a role in degrading chitin into chitin derivative compounds. The constituent components of chitin are used by various microorganisms capable of producing chitinase enzymes. One such microorganism is bacteria. Chitinolytic bacteria are known to be able to control the growth of pathogenic fungi in plants. Fungal cell walls are composed of various complex compounds including chitin, glucans, mannans and proteins. Based on the composition of these constituents, the enzymes that can hydrolyze the fungal cell wall also vary, namely chitinase, glucanase, protease and mananase. The synergy of these hydrolase enzymes can have a good pathogenic effect in lysing the cell walls of pathogenic fungi. This study aims to determine the potential of chitinolytic bacteria as biological control agents for plant pathogenic fungi. The research was conducted at the Microbiology Laboratory, University of North Sumatra. The research method used is descriptive qualitative. The chitinolytic bacteria used were derived from shrimp shell waste. There were 10 isolates of chitinolytic bacteria tested, namely NR02, NR03, NR04, NR05, NR06, NR07, NR08, NR09, NR10, NR11 and PU01. The results showed that isolates of chitinolytic bacteria from shrimp waste were able to inhibit the fungus Fusarium oxysporum.
Lidah Buaya (Aloe vera) Sebagai Obat Herbal Penyembuh Luka Dewi Permata Sari; Chanseli Siringoringo; Muhammad Komarul Huda
Journal of Natural Sciences Vol 3, No 3 (2022): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v3i3.299

Abstract

This study was carried out with the main objective of explaining the content contained in the Aloe vera plant, and its effect on the healing process of burns. The research was conducted by utilizing various literatures that are in accordance with the topic of this research. The method used in this study is included in qualitative research by collecting various literatures or information and then analyzed to obtain a relationship or linkage with the research. Through the development of this research, it can then be explained that the Aloe vera plant has various benefits, one of which is as a wound healer because Aloe vera contains saponins, tannins, mannose-6-phosphate and polysaccharides. The compounds contained in these plants play an important role in the wound healing process. Aloe vera is a plant that contains active substances that can accelerate wound healing such as glucomannan, lignin, vitamin A, vitamin C, enzymes and amino acids that are important for skin cell regeneration. With a variety of content and benefits in these plants can accelerate wound healing and closure.
Isolasi Dan Karakterisasi Bakteri Dari Tanah Bakaran Sampah Toberni S Situmorang; Rahmiati Rahmiati; Helen Anjelina Simanjuntak
Journal of Natural Sciences Vol 3, No 3 (2022): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v3i3.306

Abstract

Bacteria are microorganisms that are abundant in the environment. Isolation of bacteria has been carried out from various sources of water, air and soil. Soil is a material that is rich in nutrients needed by various organisms including bacteria, which are capable of producing secondary metabolites that can be utilized in various fields. Different sources of isolates will produce different characteristics of the bacteria. This research aims to determine the type and characterization of bacteria originating from burnt waste soil. The research was conducted using a qualitative descriptive method. The soil sample used is burnt waste soil. The results showed that 6 bacterial isolates from burnt waste soil had different characteristics, namely TBS01, TBS02, TBS03, TBS04, TBS05, TBS06. Microscopic identification results showed that the bacterial isolates TBS01 and TBS02 were gram-negative bacteria and the other isolates included gram-positive bacteria. Differences in morphological and microscopic characteristics indicate different types of bacterial isolates.
Uji Aktivitas Antijamur Ekstrak Etanol Herba Patikan Kebo (Euphorbia hirta L.) Terhadap Candida albicans Helen Anjelina Simanjuntak; Hermawan Purba; Wan Yuli Anjani; Rahmiati Rahmiati; Toberni S Situmorang
Journal of Natural Sciences Vol 3, No 3 (2022): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v3i3.303

Abstract

The patikan kebo plant is used to cure respiratory infections, malaria, liver, hypertension, gastrointestinal, skin diseases, accelerate wound healing, antidotes, hepatitis, rheumatism and tumors. The purpose of this study was to determine the antifungal activity of patikan kebo. The research method was carried out experimentally with the paper disc diffusion method. The antifungal activity test of each concentration (1%, 2%, 3%, 4%, ketoconazole, DMSO) had respective values (7.14 mm, 7.67 mm, 8.51 mm, 8.80 mm, 26.81 mm, 0 mm). The content of secondary metabolites in the patikan kebo plant are saponins, tannins, alkaloids, steroids/triterpenoids and flavonoids. The patikan kebo plant has medium category antifungal potential.
Studi Komperasi Model Pembelajaran Talking Stick dan Snowball Throwing Terhadap Hasil Belajar Berdasarkan Taksonomi Bloom Marlindoaman Saragih; Risjunardi Damanik
Journal of Natural Sciences Vol 3, No 3 (2022): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v3i3.307

Abstract

This study aims to determine the difference in learning outcomes and the magnitude of the differences in student learning outcomes using the Talking Stick and Snowball Throwing learning models on the excretion system material in class VIII SMP Swasta Bina Guna Tanah Jawa. This research is a type of quasi-experimental research that is given different treatment in each class. The research sample was taken using a saturated sampling technique (census) which consisted of 2 classes, namely class VIII-1 as experiment 1 with 32 students using the Talking Stick learning model, while class VIII-2 into experiment 2 with 32 students using the Snowball Throwing learning model, total of sample is 64 students. Data analysis was carried out by finding the mean (X ), standard deviation (S) and hypothesis testing (t test) at a significance level of = 0.05. From the results of the study, the average value of post-test experiment 1 was 86.56 and experimental 2 was 85.93 with a difference in learning outcomes of 0.63. From the results of hypothesis testing (t test) obtained tcount (1.708) t table (1.669) at the significance level = 0.05 and degrees of freedom (dk) = 32, then Ho is rejected and Ha is accepted, meaning that there are differences in student learning outcomes using the model Talking Stick and Snowball Throwing lessons.
Pengaruh Konsentrasi Maleat Anhidrida Terhadap Persen Derajat Grafting dari Polietilen Denny Akbar Tanjung
Journal of Natural Sciences Vol 3, No 3 (2022): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v3i3.318

Abstract

This research aims to determine the maximum concentration of Maleat Anhidrida in grafting into the LLDPE chain with the help of Benzoyl Peroxide as an initiator. Mixing is done by the Blending method using an internal mixing tool. The titration results showed that the maximum percentage of grafting degree occurred at a concentration of 9% Maleat Anhidrida. The formation of new peaks at wave numbers 1707 and 1267cm-1 proves that grafting has taken place into the LLDPE monomer.
Pengembangan Digital Book Dengan Aplikasi Kvisoft Flipbook Berbasis Problem Solving Untuk Meningkatkan Kemampuan Berpikir Tingkat Tinggi Siswa SMP Wina Mariana Parinduri; Titin Rahmayanti Rambe
Journal of Natural Sciences Vol 3, No 3 (2022): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v3i3.310

Abstract

Against the background of students' indifference to science lessons, student scores are still below the KKM, and students have not experienced different learning models or media. The aim of this research is to develop a science Digital Book learning media that uses Kvisoft Flipbook, based on solving problems about matter and its changes to explore the feasibility of Digital Book media obtained through expert validation and to find out the attractiveness through student responses. Types of research and development (Research and Development). Through research and development steps that are guided by the ADDIE model. The development stage involved six validators and four practitioners to assess the feasibility of the material and media. The implementation phase involved 70 students (25 students at AlMaksum Stabat Private Middle School, 25 students at Stabat 1 Public Middle School, and 20 students at Bintang Langkat Private Middle School). The validation results from material experts and media experts which were carried out in 2 stages obtained very feasible criteria with a percentage of 72.50% and media experts at 80.75%. The results obtained by material practitioners were 74.41% and media practitioners 84.71%. For students' responses to learning media in the form of problem solving-based digital books, the scores obtained were for Al Maksum Langkat Private Middle School at 80.74%, Stabat 1 Middle School at 81.22%, and Bintang Langkat Private Middle School at 80.74%.
Metabolit Sekunder Ekstrak Etanol Daun Sukun (Artocarpus altilis (Parkinson) Fosberg) dan Potensinya Sebagai Obat Junius Gian Ginting
Journal of Natural Sciences Vol 3, No 3 (2022): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v3i3.304

Abstract

Breadfruit plants have been used as traditional medicine. Parts of medicinal plants are latex, roots, bark and leaves which are used to treat fractures, sprains, diarrhea, abdominal pain, dysentery, infectious diseases, headaches, hypertension, diabetes, asthma, hepatitis, and inflammation. This study aims to determine secondary metabolites and characterization of breadfruit leaves. The research method was carried out experimentally with extraction by maceration method, and phytochemical screening on simplicia and extracts for identification of secondary metabolites. The secondary metabolites of simplicia and extracts showed the presence of flavonoid compounds, saponins and tannins. Characterization showed the presence of water content of 7.32%, water soluble extract and ethanol content of 12.29% and 8.60%, total ash content and insoluble in acid were 8.50% and 2.34%, respectively. The conclusion of the study is that the secondary metabolites of breadfruit leaves have the potential as medicinal ingredients and the simplicia characterization has met the simplicia standard.
Aktivitas Enzim Antioksidan Pada Akar Eleocharis dulcis (Burm.F.) Trin. Ex Henschel Dalam Fitoremediasi Air Asam Tambang Batubara Di Wetland Air Laya 02 Wike Agung Safitri; Juswardi Juswardi
Journal of Natural Sciences Vol 4, No 1 (2023): Journal of Natural Sciences Maret
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v4i1.333

Abstract

Acid mine drainage (AMD) formed from open pit mining can cause environmental impacts if discharged directly into the waters because it has a low pH and contains heavy metals. Attempts to neutralize the pH and absorb heavy metals in AMD can be carried out by means of phytoremediation using Eleocharis dulcis (Burm.f.) Trin. Ex Henschel. This study aims to determine the activity of PO, PPO, and CAT on the roots of E. dulcis in AMD phytoremediation and evaluate the success of AMD phytoremediation. Sampling using convenience sampling method. Measurement of metal content of Fe and Mn with atomic absorption spectrophotometer (AAS). Protein extraction using refrigerated centrifugation, measurement of total protein content with BSA, and activity of PO, PPO and CAT using a uv-vis spectrophotometer. Based on research that has been done, E. dulcis is able to increase the pH from 5.6 to 7.7 and reduce Fe levels from 7.88 mg/l to 0.25 mg/l, Mn 1.71 mg/l to 0.75 mg /l. The physiological response shown by E. dulcis in AMD phytoremediation was an increase in antioxidant enzyme activity PO 178.98 U/mg protein/minute, PPO 388.96 U/mg protein/minute and CAT 184.34 U/mg protein/minute. Based on research, E. dulcis is a plant that is tolerant to pH stress and heavy metals, so it has the potential to be used in AMD phytoremediation.

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