Berkala Penelitian Hayati
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
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<![CDATA[KEANEKARAGAMAN JENIS BENALU PARASIT PADA TANAMAN KOLEKSI DI KEBUN RAYA EKA KARYA, BALI ]]>
<![CDATA[Tahan Uji]]>;
<![CDATA[Sunaryo Sunaryo]]>;
<![CDATA[Erlin Rachman]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/316
<![CDATA[Benalu is one of the parasitic plants which have ataccked many collection plants species in Eka Karya Botanical Garden, Bali. Exploration and collection of these parasitic plants in this area are conducted. Four parasitic plants species, i.e. Dendrophthoe pentandra, Helixanthera cylindrica, Scurrula atropurpurea, and S. parasitica are recorded and they attack 32 collection plants species in Eka Karya Botanical Garden. Dendrophthoe pentandra is reported as the highest population species to parasiting collection plants species. While the Myrtaceae family and Syzygium genera are also reported as the highest parasited species.]]>
<![CDATA[KARAKTERISASI HIDROLISAT PROTEIN KEDELAI HASIL HIDROLISIS MENGGUNAKAN PROTEASE DARI TANAMAN BIDURI (Calotropis gigantea) ]]>
<![CDATA[Yuli Witono]]>;
<![CDATA[Aulanni’am Aulanni’am]]>;
<![CDATA[Achmad Subagio]]>;
<![CDATA[Simon Bambang Widjanarko]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/317
<![CDATA[Properties of soy protein hydrolysate produced by protease from biduri plant were studied. The soy protein hydrolysate had different properties due to various concentrations and hydrolysis times of protease from biduri. Enzymatic hydrolysis of the soy protein decreased significantly in the TBA value. This process increased the soluble protein content and promoted the Maillard reaction, resulting in a more brown color. Moreover, the soy protein hydrolysate had a higher value of ‘umami’ taste by organoleptic evaluation.]]>
<![CDATA[KONSTRUKSI VEKTOR BINER UNTUK EKSPRESI GEN dip22 (YANG DIISOLASI DARI TEBU VARIETAS M 442-51) PADA TANAMAN ]]>
<![CDATA[Wiwit Budi Widyasari]]>;
<![CDATA[Sony Suhandono]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/318
<![CDATA[Sugarcane is the principle plant for producing sugar in Indonesia. Water supply is one key element in the agronomy of sugarcane. Sugarcane is a high biomass crop which requires large amounts of water. Low yields of sugar observed in water stressed plants indicate that sugarcane is very sensititive to drought. A number of genes that respond to drought, salt, and cold stress at the trasnscriptional level have been reported. dip22 (drought inducible protein) protein isolated from drought resistance variety M 442-51 was predicted to be a protein regulator to water stress in sugarcane. Increasing of tolerance to water stress by over expression of dip22 genes in high yield sugarcane variety hopefully will maintain sugar production. The goal of this research was to construct a binary vector for dip22 gene expression in plant. dip22 gene from mutated PCR was cloned to pGEM®–T Easy and transformed to Escherichia coli strain DH5a. And then, these gene was isolated again from pGEM®–T Easy-dip22 (pGdip) plasmid using restriction enzymes NcoIand PmlI. pCAMBIA 1303 plasmid is an expression vector which has the constitutive promoter CaMV35S. Recombinant plasmid was transformed to Escherichia coli strain DH5a for plasmid propagation through DNA replication. Recombinant plasmid was isolated, and digested with NcoI and PmlI to examine the presence of dip22 gene in the pCAMBIA 1303 plasmid. The recombinant plasmid was transformed to A. tumefaciens strain LBA 4404. Plasmid isolated from A. tumefaciens was digested with Bst XI and Bst EII to examine the similarity between pCAMBIA 1303-dip22 (pCdip) from Escherichia coli and A. tumefaciens. The result by electrophoresis showed that both plasmids had the same size after digested. It was concluded that the transformed A. tumefaciens strain LBA 4404 bacteria has pCAMBIA 1303-dip22 (pCdip) plasmid indeed. Therefore, this construct of dip22 gene in binary vector can be used for improving drought tolerance in plant.]]>
<![CDATA[PERBANDINGAN PERTUMBUHAN DAN PERKEMBANGAN GONAD IKAN MAS (Cyprinus carpio Linn.) DIPLOID DAN TETRAPLOID ]]>
<![CDATA[Akhmad Taufiq Mukti]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/319
<![CDATA[The aim of this study were to know growth and gonadal development of tetraploidy common carp. The method that used in this study was experiment. Treatment that used was tetraploidyzation by heat shock 40°C during 1.5 minutes of common carp eggs to 29 minutes after fertilization. Five replicates were carried out for treatment. Parameters test were relative length growth (h), specific growth rate (SGR) and gonadal development of common carp. Data analysis that used was descriptive analysis. The result of this study indicated that tetraploidization teratment influenced on growth and gonadal development of common carp. The relative length growth of tetraploidy common carp was 5.38 ± 0.12 (30-days) and 19.12 ± 0.00 (110-days), as their specific growth rate was 19.87 ± 0.10% BW/day (30-days) and 8.57 ± 0.00% BW/day (110-days). Tetraploidy common carp have fertile characteristic and show gonadal development that same with diploid common carp relatively.]]>
<![CDATA[EMBRIOGENESIS SOMATIK ANGGREK BULAN Phalaenopsis amabilis (L.) Bl: STRUKTUR DAN POLA PERKEMBANGAN ]]>
<![CDATA[Edy Setiti Wida Utami]]>;
<![CDATA[Issirep Soemardi]]>;
<![CDATA[Taryono Taryono]]>;
<![CDATA[Endang Semiarti]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/320
<![CDATA[Research of the structure and development pattern of somatic embryos from callus of leaf explants moon orchid Phalaenopsis amabilis (L) Bl had been done. One year old of plantlets were used as explants sources. Basal leaf of these explants were cultured in Somatic Embryo Induction Medium (SEIM) e.i.: NP(New Phalaenopsis) medium added with 2 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Then somatic embryos were transferred to EMM (Embryo Maturation Medium) e.i.: NP medium added with 1 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Finally, mature somatic embryo were transferred to NP medium without plant growth regulator as Embryo Germination Medium (EGM). The origin of somatic embryos initially from single cell at the pheriphery of embryogenic callus. These cells then devided in mitotic repeatedly formed globular proembryo, elongation embryo, and completed embryo. The structure and development pattern of somatic embryos as the same as with zygotic embryo.]]>
<![CDATA[STUDI PERBANDINGAN METODE TRANSFORMASI DNA MENGGUNAKAN VEKTOR Agrobacterium tumefaciens PADA TANAMAN TEBU (Sacharum hybrid) ]]>
<![CDATA[Sri Setyati]]>;
<![CDATA[Purnama Oktaviandari]]>;
<![CDATA[Muhammad Hazmi]]>;
<![CDATA[Bambang Sugiharto]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/321
<![CDATA[In order to compare transient expression of gus gene driven by CaMV 35S and rice ubiquitin RUBQ2 promoters, a DNA transformation was conducted using embryogenic callus and suspension cultures of sugarcane. The transient gus expression was observed by histochemical staining method. The histochemical observation of GUS activity after co-cultivation showed that RUBQ2 promoter produced high level of clear blue spots both in embryogenic callus and suspension cultures, while the CaMV35S promoter was not detected. The suspension cultures slightly increased transient gus gene expression compared to embryogenic callus. However, the histochemical analysis of regenerated putative transformant plants after 5 successive cycles on the selection medium showed no blue spots of gus gene expression. PCR amplification of DNA for CaMV35 or nptII in putative transformant plants confirmed that there was no integration of the transformed gene in the genome DNA. The results suggested a possibility of somaclonal variation with callus propagation, thus did not produce transformed plants. To avoid the somaclonal variation, the transformation was conducted using in vitro plants and multiple shoots without intervening callus phase. Histochemical observation of infected materials after co-cultivation showed that almost all of the infected materials partially exhibited blue color in the basal region. In case of in vitro plants, they rapidly grow and multiplied in the selection medium, thus the method provided an excellent system for the transformation in sugarcane. The results suggest that in vitro plants as well as multiple shoots need further investigation to be used as target tissues for Agrobacteriummediated transformation in sugarcane.]]>
<![CDATA[UJI AKTIVITAS ANTIOKSIDAN EKSTRAK AIR DAN EKSTRAK METANOL BEBERAPA VARIAN BUAH KENITU (Chrysophyllum cainito L.) DARI DAERAH JEMBER ]]>
<![CDATA[Moch. Amrun H.]]>;
<![CDATA[Umiyah Umiyah]]>;
<![CDATA[Evi Umayah U]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/322
<![CDATA[Star apple or Chrysophyllum cainito L., family Sapotaceae; which is indigenous in Central America has been grown locally arround Jember, East Java. Ethnobotanical data exhibits its medicinal properties such as: soothing inflammation in laringitis and pneumonia, treatment for diabetes mellitus and cancer remedy which are related to free radical mechanism. Therefore, it is necessary to determine its free radical scavenger activity.There are three types of Jember’s star apple fruit (local name: kenitu): big size, green color with round shape (kenitu hijau bulat); medium size, green color with oval shape (kenitu hijau lonjong); and small size, red purplish color with round shape (kenitu merah bulat). Previous research shown that both water and methanol extracts of kenitu hijau lonjong has DPPH free radical scavenger activity as its antioxidant capacity. In recent research the DPPH free radical scavenger activity was performed on both water and methanol extract of these three types of kenitu. The results showed that kenitu merah bulat exhibits the highest antioxidant activity of these three types with IC50 = 426.118 and 169.094 ppm for methanol and water extracts sequentially in 60th minutes.]]>
<![CDATA[SEMI PURIFIKASI DAN KARAKTERISASI ENZIM PROTEASE Bacillus sp. ]]>
<![CDATA[Elidar Naiola]]>;
<![CDATA[Nunuk Widhyastuti]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/323
<![CDATA[The aim of the research was to find the partial purified of enzyme protease from Bacillus sp. The crude enzyme of protease was produce in rice brand medium (100 gram of rice brand in a liter tofu liquid waste). The enzyme was semi-purified by the procedure of precipitation using ethanol in different percentages of saturation, gel filtration using Sephadex G 100 and Ion Exchanged Chromatography using DEAE Sephadex A50. Specific activities of the enzyme during purification were 5.71 U/mg (crude enzyme); 6.75 U/mg (ethanol precipitations); 37.16 U/mg (gel filtration) and 43.02 U/mg (Ion Exchanged Chromatography). The optimum temperature for enzyme reaction was 45–50 °C, while the optimum pH was 7.0–8.0. Protease was relatively stable after heating until 37–50 °C for 60 minutes. Metal ions had different effects to the enzyme. CaCl2, FeCl3, MnCl2, ZnCl2 and MgCl2 increased enzyme activity, CdCl2 and HgCl2 gave an inhibitory effect, and another of metal ions had no effects to the enzyme.]]>
<![CDATA[PENGARUH MUTASI DENGAN RADIASI SINAR GAMMA (Co60) TERHADAP PRODUKTIVITAS JAMUR TIRAM ABU-ABU (Pleurotus sajur-caju) ]]>
<![CDATA[Ira Djajanegara]]>;
<![CDATA[Priyo Wahyudi]]>;
<![CDATA[Donowati Tjokrokusumo]]>;
<![CDATA[Netty Widyastuti]]>;
<![CDATA[Harsoyo Harsoyo]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/324
<![CDATA[Irradiation aplied to living organisms may have positive or negative effects on physiological and morphological properties of the organisms. One way to gain genetic variation with better properties than the parental strain is by Gamma (Co 60) radiation application. During this experiment, Gama (Co 60) rays was applied to the grey oyster (Pleurotus sajur-caju) mushroom mycellia during exponential phase. Radiation was applied at 0.75 KGray with dose velocity of 1.149 KGray. Analysis of mushroom productivity performances indicate that diameter of mycellia, fresh weight, dry weight, diameter of fruit body and the amount of fruit body of the mutant and control were not significantly different. However, the isozyme pattern showed a different pattern between the mutant and the control which indicates that mutation process has already occured. These data show that mutation did not affect the productivity of the mushroom. Therefore, mutation may affect the nutritional quality of the mushroom instead. Further experiment to verify this possibility is suggested.]]>
<![CDATA[IMMUNOGENESITY OF SPESIFIC PROTEIN MOLECULAR WEIGHT 16 KDa (PS16) LEAF OF SIAM CITRUS INFECTED BY CITRUS VEIN PHLOEM DEGENERATION (CVPD) DISEASE ]]>
<![CDATA[Made Sritamin]]>;
<![CDATA[Aulanni ‘am Aulanni ‘am]]>;
<![CDATA[IGP. Wirawan]]>;
<![CDATA[Liliek Sulistyowati]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/325
<![CDATA[Citrus Vein Phloem degeneration (CVPD) is an important citrus disese, which damaged citrus plantation and causing decrease of citrus production. In Indonesia, the CVPD disease caused by Liberobacter asiaticum bactery and the disease spread out by vectir insect Diaphorina citri and using infected bud in wood grafting. In infected citrus plant, two specific protein molecules with molecular weigt 16 kDa and 66 kDa are found. These protein molecules are not found in healthy citrus plant. The immunogenicity of PS16 accumulated on leaf of citrus plant infected by CVPD is known yet. The research material were leaves of citrus plant infected CVPD, leaves of healthy citrus plant and reagent used these research are for isolation of the total protein leaf of citrus plant, SDS-PAGE electroforesis, electroelution of PS16, ELISA Methods, Dot-Blot Method, anti-PS16 as aprimery antibody and secondary antibody is anti-Rabbit IgG Conjugated AP. The result of the research showed that of PS16 accumulated on leaf of citrus plant infected CVPD has immunogenic character. It is indicated by increase of the titer anti-PS16 after first immunization ang 2nd booster by indirect ELISA method and can be used to induce antibody (anti-PS16) and so showed that positive reaction between PS16 with anti-PS16. It is indicated by purples dark blue on cellulose membrane by Dot Blot method.]]>
