Berkala Penelitian Hayati
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
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507 Documents
PENGARUH PEMANGKASAN DAN PENAMBAHAN GIBERELIN (GA3) TERHADAP LAMA WAKTU PEMBENTUKAN TUNAS DAN KUNCUP BUNGA TANAMAN MAWAR (Rosa sp)
Listijani Suhargo;
Y. Sri Wulan Manuhara
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 2 (1995): December 1995
Publisher : The East Java Biological Society
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DOI: 10.23869/177
The effect of cutting and adding gibberellin on the duration of stem and flower bud forming of Rosa sp had been done. This research purpose to shorthen the duration of stem and flower bud forming. The research sample were Rose plant with yellow colour and the same length and number of branches. The cutting was done on 0 cm, 5 cm, and 10 cm from the apex. In this research, we used gibberellins with 50 ppm and 100 ppm concentrations and as a control we used aquadest. The result showed that the best treatment to shorthen the duration of stem bud forming were the cutting 10 cm from the apex and with adding of gibberellin on 100 ppm concentration. And the best treatment to shorthen the duration of flower bud forming were with the cutting on 10 cm from the apex and with adding of gibberellins on 50 ppm concentration.
STUDI MEKANISME KINETIKA REAKSI PERTUKARAN ION FE(III) ANTARA DUA JENIS LIGAN SIDEROPHORE BAKTERI (DESFERRIFERRIOXAMINE B DAN AZOVERDINE) DENGAN LIGAN EDTA
Handoko Darmokoesoemo;
A.M. Albrecht Gary
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 2 (1995): December 1995
Publisher : The East Java Biological Society
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DOI: 10.23869/178
The kinetics of iron exchange and iron removal from two siderophore complex with use of ethylenediaminetetraacetic acid (EDTA) have been examined, using UV-Vis spectrophotometric method. Iron exchange between the ferric complexes of desferriferrioxamine B (siderophore produced by microorganism Azomonas macrocy-togenes) with EDTA was extremely slow under conditions at pH 7.4 and 25oC. Furthermore, pH dependence studies demonstrated that the exchange rate was accelerated as a function of increasing hydrogen ion concentration. Kinetics of iron removal from ferrioxamine B and the ferric complexe of azoverdine with excess of EDTA, show firt-order dependence on the concentration of the iron complex and hydrogen ion at 25oC with first-order rate constant of the dissociation of a ternary complex formed of (16 ±0.4) x 10-3s-1 and (1.3 ±0.2) x 10-2s-1 respectivy at pH region between 3.67 and 5.23. variation of pseudo first order rate constant kobs with hydrogen ion concentration shows a saturation kinetics at high constant ligand EDTA concentration (3.0 x 10-2 M) and at low pH region (3.67). the result were interpreted in terms of three-step mechanism, involving : (1) protonation of the ferrioxamine B comples and the ferric comples of azoverdine and (2) subsequent biomolecular reaction with EDTA.
BIOTRANSFORMASI 2E-6E-FARNESOL OLEH KHAMIR ENDOFIT CA1C-4
Andria Agusta;
Yuliasri Jamal
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society
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DOI: 10.23869/179
The microbial transformation of 2E-6E-farnesol by the endophytic yeast CA1C-4 isolated from temu hitam (Curcuma aeruginosa Roxb.) has been investigated. Incubation of the 2E-6E-farnesol in cultivated endophytic yeast CA1C-4 in PDB at room temperature (25–32o C) under shaking condition at 120 rpm for eight days, produce a biotransformed product. Chemical structure elucidation based on 1H- and 13C-NMR analysis and comparison with published data showed that the biotransformed product is 2E,6E-3,7,11-trimethyl-2,6,10-dodecatrien-1-carboxilic acid.
COMPOSITION OF UNDER-SHRUBS SPECIES IN MOUNT SALAK NATIONAL PARK, WEST JAVA
Inge Larashati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society
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DOI: 10.23869/180
Forest in Mount Salak area has a very important existence, so that in the year of 2003 was announced officially as a conservation area and became a part of Mount Halimun Salak National Park (TNGHS). As one of the conservation area in Indonesia, Mount Salak forest has so many natural resources which support the living prosperity of the people, especially The West Java citizen. But in the use of the natural resources people have never been appreciating the conservation concepts then causing the forest degradation rapidly and terribly saddening. In order to explore the vegetation in the conservation area left, then the ecology analysis was done by making research plots in a hectare area. Data collecting was done on the systematically-located 1 meter square-size sub-plots. Those plots were located in a relatively-good natural forest. Data analysis result stated Athyrium dilalatum, Stenochlaena palustris, Calamus javensis, Curculigo latifolia and Strobilanthes blumei were the species which dominated Mount Salak National Park in the altitude of 1.267 meter above sea level.
EFEK TEPI KORIDOR JALAN DI HUTAN BUKIT POHEN, CAGAR ALAM BATUKAHU, BALI
Dini Fardila;
Sutomo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society
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DOI: 10.23869/181
Road corridor has been known to have an influence on the microclimate and vegetation condition along the forest edge which directly bordered with the road. This study was conducted in order to obtain information regarding the depth of edge influence which can be seen by the changes in microclimate and vegetation condition from forest edge to interior in Pohen Mountain, Batukahu Nature Reserve Bali. Microclimate variables such as light intensity, wind velocity, temperature and relative humidity, and vegetation variables such as basal area, tree height, density and number of species were measured on two 100 m transects perpendicular from forest edge to interior. The correlation between microclimate and vegetation condition with distance from the road was analyzed by using MANOVA and ANOVA. The depth of edge influence was estimated by using Principal Component Analysis (PCA) ordination. Microclimate showed significant correlation with distance from the road to forest interior (r > 0.5, p < 0.05). Light intensity, temperature and wind velocity tends to decline as we move towards the forest interior and the reverse was true for the relative humidity. Microclimate gradient changed sharply until it reached the distance of 40 m from the forest edge. For vegetation parameter only the height of trees which was signifi cantly correlated with the distance from the road. PCA analysis showed that the depth of edge influence for the microclimate condition was clearly showed up until 40 m from the road. This result suggests that forest buffer area with minimum width of 40 m is needed to protect the forest interior of Pohen Mountain from the exposed microclimatic condition of the edge area.
POPULASI DAN AKTIVITAS DENITRIFIKASI SERTA EMISI GAS N2O PADA LAHAN PERTANIAN ORGANIK, PERTANIAN INTENSIF, DAN HUTAN
Dwi Agustiyani;
Nur Laili;
Hartati Imamuddin;
Nunik Sulistinah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society
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DOI: 10.23869/182
This research investigate the population and potentials denitrification activity from three different soils, organically farmed soil, intensive farmed soil and forest soil. Our objectives were to explore spatial gradients in denitrifier populations, examine whether populations density and its potential activity was related to soil chemical properties (C and N content), and determine the potential emission of gas N2O. Results indicated biological functional differences between these three different soil ecosystems. Forest soil had the highest population density of denitrifying bacteria and also had significant potential denitrifying activities. The highest potentials denitrifying activity in the soil affected to the lowest emission of N2O gas. The lowest population and potential denitrifying activity was measured in the intensive farmed soil. Those conditions might be promoted the potentials emission of N2O.
MITOCHONDRIAL DNA (MTDNA) VARIATION OF SULAWESI BLACK MACAQUE (Macaca nigra) LIVED IN TWO NATIONAL PARKS (TANGKOKO AND DUA SAUDARA) OF NORTH SULAWESI
Hapry F.N. Lapian
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society
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DOI: 10.23869/183
Hypervariable II (HVII) regions of mitochondrial DNA (mtDNA) D-loop regions of Macaca nigra lived in Tangkoko and Dua Saudara National Parks have been analysed. An approximately 600 base pairs (bp) of HVII regions was sequenced to characterize genetic variation among 20 samples of Macaca nigra lived in Tangkoko and Dua Saudara National Parks. Using an outgroup of Macaca fascicularis of Kalimantan, a neighbor-joining and maximum parsimony trees both identified that all DNA samples of Macaca nigra were arranged in similar cluster. The low nucleotide diversity among samples of Macaca nigra in Tangkoko and Dua Saudara suggests either there is a direct mix among species or that they may have similar ancestor among them.
PENGUJIAN KOMPOS DAN INOKULAN MIKROBA TERHADAP PERTUMBUHAN TANAMAN SENGON BUTO (Enterolobium cyclocarpum, (WILLD) PADA LAHAN BEKAS TAILING POND DI CIKOTOK
Hartati Imamuddin;
Nunik Sulistinah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society
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DOI: 10.23869/184
Soil pollution has been so attracting considerable public attentions over the last decades. Phytoremediation is an emerging technology that uses plants to clean up pollutant soils. The study was carried out in gold mining Cikotok, Banten. Sengon buto (Enterolobium cyclocarpum, (Willd) which is used as cyanogenic plant, compost and microbes inoculant is as stimulator to growth this plant. The experiment consist of K0 as control (plant without compost), K1 as plant + compost and K2 as plant+compost + microbe inoculant, with 3 replicated. The objectives of this experiment were to investigate the potential for phytoremediation of cyanide contaminated soils using hyperaccumulator/cyanogenic plants and to assess the fate and transport of cyanide compounds in soils. The results showed that compost and microbe were able to stimulate growth of Sengon Buto after 7 months planting and to reduce cyanide until 66% Total bacteria in the study was relatively stable but NFB bacteria was decline.
AMOBILISASI SEL Bacillus licheniformis KA-08 DALAM MENGHASILKAN KERATINASE TERMOSTABIL
Anthoni Agustien
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society
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DOI: 10.23869/185
Isolate local Bacillus licheniformis KA-08 known extracellular thermostable keratinase producers. Scale up of thermostable keratinase production can be with cells immobilized. The objective of the research is to thermostable keratinase production of B. licheniformis KA-08 cells immobilization. Thermostable keratinase activities were determined with modification of Brandelli and Riffel method. Protein concentration of enzyme determined with Lowry method. Immobilization of cells by Ca-alginate matrix with Adinarayana method, alginate concentration and amount of alginate bead effects with Beshay method. The result extracellular thermostable keratinase of B. licheniformis KA-08 cells immobilized was maximum produced at 12 times incubation with activity as 9.25 U/mg. Three percent alginate has optimum activity. Three hundred alginate beads has optimum activity. Cells immobilized of B. licheniformis KA-08 has scale up of thermostable keratinase activity at 2 times than free cells. Thermostable keratinase produced by cell immobilized was nine cycles.
DIVERSITAS SERANGGA PADA HUTAN TANAMAN MONOKULTUR DAN HUTAN HETEROGEN DENGAN METODE WINDOW TRAPS
Irham Falahudin;
Jasmi;
Siti Salmah;
Dahelmi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society
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DOI: 10.23869/186
Insects are a very important role in an ecosystem. Among these roles are: herbivori, predation, parasitism, decomposition, pollination, and so on (Speight et al., 1999). In addition to the role of insects have been used as an indicator species. Several recent studies (Davids, 2000; Talvi, 2000; Castillo and Wagner, 2002; McGeoch, 1998 & 2002) shows that insects have a response that indicates the extent of damage to forest habitat. To see how much effect the change will see the diversity of insects flying at both locations using the window trap. This method was chosen because it will look at the role of flying insects that can be used as indicators of changes in land to the stratification of the existing trees at both locations. The expected goal in this study were 1) To determine the level of insect diversity in both forest site 2) To determine the effect of changes in forest insect conditions in each forest stratification. From this research it was found that it contained the highest density of heterogeneous forests there are 29 families of 41 species and 119 individuals from arrest (day and night) while in monoculture forests with 32 species of 22 families and 98 individuals in which the degree of diversity between the forest with a monoculture in the heterogeneous forest monoculture forests range 2.14