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Sutiman Bambang Sumitro
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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 507 Documents
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PENGARUH JENIS BAHAN PEREKAT DAN METODE PENGERINGAN TERHADAP KUALITAS BRIKET LIMBAH BAGLOG JAMUR TIRAM PUTIH (Pleurotus ostreatus) Siti Mushlihah; Sulfahri; Renia Setyo Utami; Eko Sunarto; I.D.A.A. Warmadewanthi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.967 KB) | DOI: 10.23869/187

Abstract

White mushroom is one of popular food commodity in East Java. Every year total production of white mushroom is more than 4800 ton. The cultivation of this mushroom needed the medium known as "baglog". Every 3 month the medium should be changed. The huge amount of solid waste will be produced around 40 tons/month and must be managed by farmer. Based on the composition of waste contains 80% of sawdust and 10% of rice brain. This composition known has a high concentration of carbon and potential as a material to produce of energy. This research investigated the effectiveness of "baglog" from medium of white mushroom cultivation as a briquette for energy alternative. Different variables used in this experiment are drying process and material that used as an adhesive for making of the briquette. The quality of the briquette such as energy value, compressive strength of briquette, moisture, fixed carbon and emission of briquette, was analyzed, based on Indonesia Standard. Result of the experiment showed that energy value of the briquette is 3400 cal/gram with drying process using oven. The compressive strength result showed that the adhesive using starch glue is very effective. This briquette is environmentally friendly
TOKSISITAS ASETAMINOFEN PADA KHAMIR Candida tropicalis Heddy Julistiono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.652 KB) | DOI: 10.23869/188

Abstract

Use of the yeast for cell based method to do a screening substances having anti-oxidant property is considered to be simpler and cheaper. In order to develop yeast Candida tropicalis as a tool for evaluation of anti- or pro-oxidant property of substances in cell level, toxicity of an analgesic drug acetaminophen in the yeast had been preliminary studied. Incubation of yeast cell suspension in presence of 0.3% acetaminophen for 2 hours significantly decreased cell viability. Malon dialdehyde, a biochemical marker for cell oxidative damage, increased. Acetaminophen of 0.1% or 0.039% decreased when added in cell yeast suspension or supernatant respectively for 1 hour indicating drug metabolism by cellular and extracellular enzymes. The data indicated that toxicity of the drug in the yeast could be compared to that in mammalian cell where the drug was metabolized by cytochrome P-450 or peroxidase and followed with oxidative stress in cells caused by metabolite byproduct. Toxicity of the drug in the yeast may be in relation with formation of reactive oxygen species. These preliminary data used the yeast for screening antioxidant property of substances against acetaminophen toxicity.
ISOLASI DAN KARAKTERISASI BAKTERI PENDEGRADASI HERBISIDA DIURON DAN BROMACIL DARI AREA PERKEBUNAN DI LAMPUNG Nur Laili; Hartati Imamuddin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.778 KB) | DOI: 10.23869/189

Abstract

Diuron and bromacil are a broad-spectrum herbicide that is widely used in some plantation at Lampung. The soil sample is collected from some locations such as at banana Plantation (NTF), pineapple plantation (GGPC) and sugarcase plantation (GM). The aims of the research was to know the isolation and characterization of diuron and bromacil degrading bacteria. The highest diuron resistant bacteria was found in GM soil sample and The highest bromacil resistant bacteria was found in NTF soil sample. The result showed that there are three isolates for diuron degradation and five isolates for bromacil degradation bacteria from NTF, GGPC and GM. These isolates were tested to explore their ability for diuron and bromacil degradation on different concentrations. Isolation of bacteria was tested by using enrichment culture method and degradation of diuron and bromacil were checked by using spectrophotometric method. Diuron and bromacil degradation by 5 consortium indicated that all of consorsia have the ability to degrade them.
PENGARUH KEMASAN DAN LAMA PENYIMPANAN Acetobacter sp. RMG-2 DALAM BAHAN PEMBAWA TERHADAP POPULASI DAN PRODUKSI BIOSELULOSA DENGAN KONSENTRASI INOKULUM TERBATAS Ruth Melliawati; Nuryati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (784.146 KB) | DOI: 10.23869/190

Abstract

Bioselulosa or nata de coco is one food product that contains 25% fiber, which is very useful to support health, especially the digestive system. Along with the development of science and technology, raw material for making nata de coco become increasingly diverse as well as developing its usefulness for the wider interests. Inoculum became one of the things that are important in nata production process is also a determinant of product quality. This research was conducted to obtain information about the packaging is good for storing inoculum Acetobacter sp. RMG-2 in the carrier material and the efficiency of inoculum for the production bioselulosa. Two kinds of containers (straws and plastic bags) with two kinds of carrier material (cellulose pulp and CMC) are used. Storage of inoculum carried out for 20 weeks at 4° C. The measured parameter is the number of population, wet weight, dry weight and thickness as well bioselulosa pesentase minimal inoculum for the manufacture bioselulosa. Result, the inoculum in the CMC are packed using a straw is better than the plastic bag packaging, with a population of Acetobacter sp RMG-2 at 4.53 x 108 cells/ml, while in the plastic bag packaging population reached 3.5 x 107 cells/ml, respectively during storage was 20 weeks (five months). Bioselulosa production uses between 1–15 ml inoculum in 1250 ml of medium/tray of wet weight data obtained vary. With 1 ml of inoculum produced an average wet weight of 725 grams with a thickness of 0.6 cm. Research on the percentage of inoculum between 0.1 to 1.0%, showing that the use of 0.1% (in the CMC carrier materials for 8 weeks) can still be used for production with the average bioselulosa/100 ml of medium weight 31.45 grams wet, 1.6 g dry weight and thickness of 0.8 cm during the 7 days incubation. Investigation was still continuing to be carried out to get optimal results.
PENAPISAN FITOKIMIA DAN ANTIBAKTERI EKSTRAK METANOL MANGROVE (Excoecaria agallocha) DARI MUARA SUNGAI PORONG Asep Awaludin Prihanto; Muhamad Firdaus; Rahmi Nurdiani
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.913 KB) | DOI: 10.23869/193

Abstract

The aims of this study were to screen phytochemical and antibacterial potency of methanol extract of Excoecaria agallocha. The phytochemical screening used qualitative analysis and the antibacterial assay determined by diffusion method. The results showed that extracts of leaf, flower, bark, stem and root of Excoecaria agallocha, respectively, contained alkaloid, steroid, flavonoid, tannin, terpenoid and saponin. Methanol extract of all part of Excoecaria agallocha, except the root, inhibited the growth of gram positive bacteria (Staphylococcus aureus ATCC 9144), Meanwhile, the leaf of Excoecaria agallocha extract not only inhibited the growth of gram positive bacteria (Staphylococcus aureus ATCC 9144) but also the growth of gram negative bacteria (Escherichia coli ATCC 8739).
EFEK PROTEKSI EKSTRAK AIR PANAS BUAH MAHKOTA DEWA Phaleria macrocarpa (SCHEFF.) BOERL. TERHADAP STRES OKSIDASI AKIBAT FERRI SITRAT PADA KHAMIR Candida tropicalis Heddy Julistiono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.103 KB) | DOI: 10.23869/194

Abstract

Yeast Candida tropiclis had been used as a cell model to investigate effects of drugs on cell level. Phaleria macrocarpa (Scheff.) Boerl., is traditionally used in Indonesia as medicinal plant. This study was to evaluate the antioxidant property of P. macrocarpa in cell level by using yeast C. tropicalis induced with ferric citrate. Ferric citrate of 1 mM or 5 mM induced oxidative stress in the the yeast, marked with increasing of an oxidative damage marker, malon dialdehyde (MDA). Concentration of 5 mM of Ferric citrate caused cell mortality but concentration of 1 mM did not affect cell viability. Hot water extract of P. macrocarpa of 1 mg/ml attenuated MDA level in yeast cell induced with 1 mM ferric citrate. Whereas, 1 mg/ml of P. macrocarpa decreased cell mortality of the yeast induced with 5 mM ferric citrate without decreasing level of MDA. However, 4 mg/ml of P. macrocarpa induced oxidative damage in yeast cell. The data may indicate the potential use of Indonesian traditional plant P. macrocarpa as herbal medicine for protecting human cell from oxidation damage.
APLIKASI RHIZOBIUM DAN CENDAWAN MIKORIZA ARBUSCULA (CMA) TERHADAP PERTUMBUHAN TANAMAN KACANG TANAH (Arachis hypogeal) DI DESA SOCAH KECAMATAN SOCAH KABUPATEN BANGKALAN MADURA Tutik Nurhidayati; Nurul Jadid; Safita Meridian
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.892 KB) | DOI: 10.23869/195

Abstract

The cultivation of peanuts in Socah village mostly located in marginal land. On the other hand to increase the productivity of land by local communities is use of chemical fertilizers. The effects of chemical fertilizers is the decline in the productivity of the land. In these circumstances it is absolutely necessary to perfect technology of dry land resources is essential. One of the technologies that can be developed is the use of rhizobium and mycorrhiza. Thus occurred the tripartite relationship between rhizobium, mycorrhiza, and plants arbuscula beneficial for growth and development of land. In this study the application of local rhizobium and mycorrhiza on crops of peanuts, with measurable parameters of nodule formation and detection of plant growth and productivity of peanut.
PENGUJIAN BIJI KOLEKSI KEBUN RAYA PURWODADI Rony Irawanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (750.334 KB) | DOI: 10.23869/196

Abstract

Purwodadi Botanic Garden is a conservation institution of ex-situ plants which goes to do conservation, research, education and ecotourism activities. The main characteristic of the botanic garden is the collection of plants, some supported collections, and their documentation. Seed collection is one of the supported collection, which is responsible for handling the management of seed material collections. Seed collection as an active collection needs to be tested to know the quality of seeds during storage (viability/germination). Seeds viability activity is routinely done in the Purwodadi Botanic Gardens. This research aims to know the quality of seed collections in the Purwodadi Botanic Gardens where the result can be used as a guidance in to do the later conservation of the seed in rider to improve the quality of Purwodadi Botanic Garden collection. The stocktaking result of the plants which produce fruit and seed showed that there are 524 species from 77 genus, with a range of seed collection is around 10 – 65 species every year. Thus, seed collection viability in 2010 amounts 64 species.
THE ORGANOLEPTIC AND PHYSIC CHARACTERISTICS AND LACTIC ACID CONTENTS OF YOGHURT WITH COMMERCIAL STARTER ADDED Bifidobacteria bifidum Tatik Khusniati; Abdul Choliq; Rita Dwi Rahayu
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.631 KB) | DOI: 10.23869/197

Abstract

Bifidobacteria bifidum is probiotic bacteria which inhibit negative bacteria in human ulcer. Adding B. bifidum in commercial yoghurt starter, may increase yoghurt quality. To know yoghurt quality, organoleptic and physic characteristics and lactic acid contents of yoghurt with commercial starter added B. bifidum was observed. B. bifidum concentrations added were 1:4, 2:4, 3:4 (v/v). Organoleptic characteristics were conducted by 18 panelists, physics were visually detected and lactic acid contents were by titration method. The results show that accepted yoghurt characteristics were yoghurt with commercial starter added B. bifidum 1:4 (v/v), and fat yoghurt were more acceptable than that skim. The higher B. bifidum concentrations used, the stronger flavours (after expiry date) and colours (at and after expiry date) of yoghurt, while yoghurt homogenity decreased (at and after expiry date). Fat yoghurt flavours were stronger than that of skim. The higher B. bifidum concentrations and storage times, the higher yoghurt lactic acid contents. Lactic acid contents of fat yoghurt with various starters, were higher than that skim at storage 0-15 days. The fat yoghurt lactic acid contents were 0.99%-1.44%, while that skim were 0.95-1.20%. Based on organoleptic and physic characteristics and lactic acid contents, fat yoghurt were more acceptable that that skim.
PROTEASE ACTIVITIES OF SEMI-PURIFIED Pseudomonas fluorescens IN PROTEIN DEGRADATION OF PASTEURIZED MILK AT STORAGE Tatik Khusniati; Irma Normalia
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.162 KB) | DOI: 10.23869/198

Abstract

Protein on stored milks spoiled due to protease activities of Pseudomonas fluorescens. To know protease effect on milks, protease activities of semi-purified P. fluorescens on protein degradation in stored pasteurized milks were detected. Protease was semi-purified by ethanol 70%. Protease activities were detected by modified Lowry method, protein degradation by formol titration, and protein content by Kjeldahl method. Milk storage' times were conducted on 0 day (4 days before expired date) up to 12 days (8 days after expired date). The results show that the longer the storage times the higher protease activities and protein degradation of milks. At storage 12 days, protease activities on control were 0.2556 Unit/mL (skim) and 0.2116 Unit/mL (whole), and on inoculated milk (crude) was 2.2044 Unit/mL (skim) and 1.5378 Unit/mL (whole); while on inoculated milk (semi-purified) was 3.5355 Unit/mL (skim) and 1.6778 Unit/mL (whole), respectively. The decrease of inoculated milk' homogeneous were faster than that of control. Protein degradation on control, inoculated skim milks (crude and semi-purified) on 12 days were 4.48%, 7.28% and 7.62%, while that on whole milks were 3.81%, 7.28% and 6.04%, respectively. Based on protease, protein degradation and homogeneous, it can be concluded that skim milk spoiled faster than whole milk.

Page 16 of 51 | Total Record : 507

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