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Sutiman Bambang Sumitro
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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 507 Documents
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ANALISIS KANDUNGAN KUMAN ENTERIK PADA AIR KEMIH Agus Supriyanto; Noer Moehammadi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 1 (1995): June 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.648 KB) | DOI: 10.23869/165

Abstract

This research was conducted in order to know the total late count (TPC) and what kind of enteric bacteria were isolated from urine specimens. Samples were taken from clinical laboratory, and was examinated in the section of microbiology department of Biology, FMIPAUniversitas Airlangga Surabaya. Thirty five samples were examinated. Method of detection of enteric bacteria, carried out in several steps including enrichment, isolation and identification. Identification step, was done using test of fisiology, motility and morphology with the Gram staining. The result showed, that the number of bacteria in urine specimens was 10 x 103 CFU per ml to 296 x 107 CFU per ml and consist of Enterobacter sp., Pseudomonas sp., Klebsiella sp., Serratia sp., Poteus morgagni, Proteus mirabilis, Aeromonas sp. and Escherichia coli.
PENGARUH KADAR AIR TANAH TERHADAP BIOMASSA CACING TANAH Pontoscolex corethrurus Alfiah Hayati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 1 (1995): June 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.428 KB) | DOI: 10.23869/166

Abstract

This research was done in order to study of effect the soil humidity on earthworm Pontoscolex corethrurus biomass. 80 earthworms were placed in plastic pot (12.5 cm in diameter and 10 cm on deep) contained soil media (four individuals in each pot). Four treatment of humidity (45 %, 5 %, 55 % and 60%) with five replication was carried out up to six weeks. At the end of experiment animals were weighting. The difference between pre and post experimental body weigh was calculated, F test was used to test biomass differences among the four conditions of humidity. The result shown that the optimum biomass was in 50% soil humidity in the soil media.
OPTIMALISASI KONDISI AMOBILISASI GLUKOMINASE MENGGUNAKAN PADATAN PENDUKUNG BENTONIT BENTUK ION UNTUK KONVERSI PATI MENJADI GULA Afaf Baktir; Sri Sumarsih; Hamami
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 1 (1995): June 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.807 KB) | DOI: 10.23869/167

Abstract

Bentonit is a clay available in a lot of quantity and very chief. It can be activated to cation or anion exchanger like resin. His research was done in order to search a treatment and to get optimum condition for binding the glucoamylase to the bentonit, and to observe the ability of immobilized glucoamylase obtained in hydrolyzing amylum. It was done three ways to activate the bentonit, to obtain three kinds of bentonit, those are 'active bentonit' (adsorbs enzyme physically) and 'cation exchanger bentonit' consist B- NH4+, and B-H+ (bind enzyme ionically). The bentonit was contacted to glucoamylase in various condition to search the optimum condition (pH, concentration, temperature, and contact duration time). Immobilized glucomynase obtained was tested it's binding strengthness and the ability of hydrolyze amylum to glucose. The conclusion of this research were, (1) bentonit can be used as a supporting material for immobilizing glucomynase in the form of B-H+ cation exchanger, (2) the optimum condition of bentonit and glucomylase binding was in pH = 5.5 and at 25oC, contact duration time was 30 minutes, (3) immobilizes glucomylase column (height of 1 cm and diameter of 2.5 cm) had conversion ability at the level of 19.88%.
STRUKTUR MOLEKUL SENYAWA ASAM 3,4 SECO BRIONONAT DALAM KULIT BATANG TANAMAN KECAPI KERA Sandoricum emarginatum Hiern Pratiwi Pudjiastuti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 1 (1995): June 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.443 KB) | DOI: 10.23869/168

Abstract

Kecapi kera (Sandoricum emarginatum Hiern) is a plant grow in Sumatera, Indonesia bong the familia Meliaceae. There are two species of Sandoricum in Indonesia, Sandoricum koetjape and Sandoricum emarginatum. The S.koetjape plant has been investigated and contains some chemical compounds and there are two of which have a cytotoxic activity to the cell culture P 388 but Sandoricum emarginatum not yet investigated. The aim of this research was to determine the molecular structure compound of stem bark of Kecapi kera which soluble in chloroform. The chemical compound in stem bark of Kecapi kera was extracted with petroleum ether, chloroform, and methanol, respectively. Chloroform fraction was separated and isolated with column chromatography, n-hexane-ethyl acetate as mobile phase and silica gel as stationary phase. Molecular structure determination was carried out using spectroscopy method such IR, NMR, and MS. As the result a white crystal, 3,4-seco bryo-nonat could be isolated. The crystal has a needle like form and had melting point between 226-228oC.
PENGARUH PEMETIKAN PUCUK DAN SAMPING TUNAS TANAMAN TEMBAKAU (Nicotiana tabacum L.) TERHADAP KERUSAKAN DAUN Hani Sudarmanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 1 (1995): June 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (412.629 KB) | DOI: 10.23869/169

Abstract

Tanaman tembakau merupakan semak setahun yang telah lama dikenal dan dibudidayakan di Indonesia. Menurut penggunaannya, dibedakan tembakau sigaret/virginia, tembakau cerutu/vorstenlands, dan tembakau rajangan. Tembakau cerutu ditanam pada musim penghujan, sedangkan tembakau sigaret dan rajangan ditanam pada musim kemarau (Utami dkk, 1993). Dalam budidaya tembakau, kualitas daun lebih diutamakan daripada kuantitasnya. Kualitas tembakau dapat dilihat dari warna, aroma, rasa, dan keutuhan daunnya. Aroma dan rasa daun tembakau ditentukan oleh kandungan kimianya, di antaranya kadar nikotin. keutuhan daun ditentukan oleh ada-tidaknya serangan serangga hama. kadar nikotin daun dapat ditingkatkan dengan pemetikan pucuk dan tunas samping tanaman tembakau. Hasil penelitian Fahrurozi (1983) sebagaimana termuat dalam Berita Biologi 2 (1), 1983, menunjukkan bahwa pemetikan pucuk dan tunas samping tanaman tembakau dapat meningkatkan kadar nikotin rata-rata 3.935% dibandingkan tanaman tembakau yang tidak dipetik pucuk dan tunas sampingnya (1.657%). Yang menjadi permasalahan adalah apakah pemetikan pucuk dan tunas samping tanaman tembakau tersebut juga dapat menurunkan tingkat kerusakan daun yang diakibatkan oleh serangan serangga hama. Hal itu dapat diakibatkan dengan fungsi nikotin (alkaloid) sebagai senjata kimiawi khususnya insect repellents (Ehrlich and Raven,1967). Asumsi yang digunakan adalah peningkatan kadar nikotin dapat menurunkan toleransi serangga hama terhadap kadar nikotin tinggi, sehingga tingkat serangan serangga hama menjadi berkurang
Evaluation of soil fungi producing dyes for dyeing cloth with mordanting alum and copper sulphate Suciatmih Suciatmih; Yuliar Yuliar
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 2 (2018): June 2018
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (507.439 KB) | DOI: 10.23869/171

Abstract

Isolation of soil fungi was done to find an enviromental friendly dyes for dyeing cloth. The aims of this study include isolation and identification of soil fungi from burning waste, cassava rhizosphere, and compost in Cibinong Science Center Area; and evaluation of dyes potential produced by the soil fungi in combination with alum and copper sulphate mordants. Thirty-six isolates of the soil fungi were successfully isolated from the burning waste, cassava rhizosphere, and compost. These include Aspergillus, Colletotrichum, Emericella, Eurotium, Fusarium, Mucor, Paecilomyces, Penicillium, Rhizopus, and Trichoderma. The soil fungi were dominated by asexual morph (77.78%) of which Aspergillus (3 species and 15 isolates) was found as the most common genus from the three samples. A total of 14 isolates were found potential as dye-producing fungi. These include Aspergil-lus sp. strain R-2 (1 isolate), A. fumigatus (3 isolates), Penicillium sp. strain GRC-1 (5 isolates), Penicillium sp. strain R-3 (1 isolate), Penicillium sp. strain RC-1 (3 isolates), Trichoderma harzianum (1 isolate); and Monascus purpureus (1 isolate; as control). Color intensity produced by fungal dyes in combination with alum and copper sulphate as pre-mordanting agents is higher than alum and copper sulphate as post-mordanting agents.
Alanine as natural biopesticide from Mirabilis jalapa and its interaction with glutamate as an inhibitor in insects immune system Dina Maulina; Mohamad Amin; Sri Rahayu Lestari; Muhammad Aziz
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 2 (2018): June 2018
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (506.751 KB) | DOI: 10.23869/172

Abstract

Alanine is a secondary metabolic secretion from Mirabilis jalapa. It is used as a modulation of natural pesticide compound. Alanine is predicted to act as an inhibitor compound in the defense mechanisms in the insect body. This study investigated the modeling structures and the protein receptor from alanine in the immune system mechanism and their optimization of the effectiveness as biopesticides to the insects. This research was conducted using an in silico modeling through the reverse docking stage, including the collection of 3D structures of natural compounds, prediction of target protein, receptor profiling, clarification of the potential of alanine compounds based on mode of action with PyRx 0.8 software, and interaction visualization between alanine compounds with the target protein using PyMOL and LigPlus+ software. The results showed that the alanine compounds from M. jalapa plants have targeted well the glutamate receptors in the insects. Both alanine and glutamate compounds have the same interactions with the glutamate proteins receptor shown by the Van der Waals interaction, e.g. the hydrogen bonding on certain amino acids has binding affinity of -15.889 kJ/mol. The interaction between alanine and glutamate affects the signal transduction cascade at the cellular level due to a neuromuscle and olfactory inhibitor. In addition, the continuous interruption of signal transduction affects the dysfunctional immune system of the insect body leading to their mortality.
PENGARUH PENAMBAHAN ENZIM DAN WAKTU INKUBASI TERHADAP JUMLAH PROTOPLAS MESOFIL DAUN ANGGREK Dendrobium Sp Edu Setiti Wida Utami
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 2 (1995): December 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.652 KB) | DOI: 10.23869/174

Abstract

This research was done in order to study of effect the supplementation of enzyme and incubation time for the protoplast quality leaf mesophyll Dendrobium sp. Protoplast can be used for culture protoplast, for somatic cross, biology research, and material for genetic manipulation. This research to make use of material was Dendrobium sp Orchid. Explant that to used was leaf mesophyll Dendrobium sp. For the protoplast isolation to used enzyme combination cellulase and maserozym (Onozuka R-10). Yakult JONSHA Co., Ltd., (Japan) with concentration cellulase : maserozym is (0.5; 0.05; 0.75; 0.075; 0; 0.1). leaf mesophyll Dendrobium sp. to get a soak in enzyme solution with incubation time 12-13 hours and 14-1 hours.the result shown that enzyme combination selulase and maserozym can be used for protoplast isolation leaf mesophyll Dendrobium sp Orchid. The best enzyme concentration to fit for isolation protoplast leaf mesophyll Dendrobium sp to be selulase 0.75% and maserozym 0.075% with incubation time 14-15 hours.
APLIKASI BEBERAPA INOKULAN Rhizobium DAN INTERVAL PEMOTONGAN HIJAUAN PAKAN TERNAK Stylosanthes guyanensus TERHADAP KANDUNGAN NITROGEN TANAH DI LAHAN KERING Kusriningrum Rochiman
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 2 (1995): December 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (481.339 KB) | DOI: 10.23869/175

Abstract

This research on discovering compatible Rhizobium inoculum for Stylosanthes guyanensis, the influence of defoliation interval on forage and the influence of interaction between inoculum and defoliation interval on nitrogen content in dry land had been conducted in Ranuklindungan Village, Grati, Pasuruan since July 1993 until November 1994. This experiment used factorial with randomized block design, with two factor (Rhizobium inoculum : without inoculum, inoculum of Stylosanthes guyanensis planted soil, legin of Leguminosae Cover Crops (Calopogonium mucunoides), Legin of Glycine max ; and defoliation interval ; without interval defoliation, 40 days defoliation interval, 60 days defoliation interval, and three replications. The inoculation which was manifested on nodule formation influences the nitrogen content in soil on 12 month old. Stylosanthes guyanensis with the best result on inoculation of Stylosanthes guyanensis planted soil. The defoliation interval doesn't influence the nitrogen content in soil. There are no interaction between inoculum and defoliation interval on nitrogen content in soil. The 4-8 month-old Stylosanthes guyanensis need supplementary nitrogen of soil media, the 8-12 month-old ones can increase nitrogen content of soil media up to 17.39 % by inoculation of Stylosanthes guyanensis planted soil and up to 12.58 % by 60 days defoliation interval.
FRAKSINASI AMILASE DARI Endomycopsis fibuligera ITBRcc64 DENGAN METODE PRESIPITASI AMONIUM SULFAT Afaf Baktir
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 2 (1995): December 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.749 KB) | DOI: 10.23869/176

Abstract

Endomycopsis fibuligera ITB.R.cc.64 produced high extracellular amylase activity. It showed two kinds of amylase activities, those are liquefication and saccharification. The aim of this research are to separate each kind of amylase by fractionation with ammonium sulfate and to test the specific activity of each fraction. It was done three fraction ammonium sulfate: 0-40 %, 40-60 %, and 60-80%. This fraction has the strong liquefication activity of 20.847 U/mg protein, the second fraction has the strong saccharification activity of 18.903 U.mg protein and the weak liquefication activity of 1.756 U/mg protein.

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