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Berkala Penelitian Hayati

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Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur

ISSN : 08526834 EISSN : 2337389X DOI : https://doi.org/10.23869/bphjbr

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 507 Documents

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KAJIAN HISTOLOGI OVARIUM IKAN SIDAT, Anguilla bicolor McClelland, YANG TERTANGKAP DI SEGARA ANAKAN CILACAP Farida Nur Rachmawati; Untung Susilo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

The aim of this research was to know the histology of eel ovary, Anguilla bicolor McClelland, at various gonad developmental stage, which caught at Segara Anakan Cilacap. This research was conducted with survey method, there are 69 samples, about 47.1–76.5 cm in length and 143.6–982.5 g in weight. The parameters are gonad weight, histological structure and gonad maturation level. The result show that all of eels is female, which in yellow eel, and presilver stage. As the result, it can be concluded that Anguilla bicolor McClelland which caught at Segara Anakan Cilacap is immature, whereas mature stage of eel is not found.

REGULASI OSMOTIK DAN NILAI HEMATOKRIT IKAN NILA (Oreochromis sp.) PADA MEDIUM DENGAN SALINITAS DAN TEMPERATUR AIR BERBEDA Untung Susilo; Wahyu Meilina; Sorta Basar Ida Simanjuntak
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

Osmotic regulation and blood hematocrite of nile, Oreochromis sp., have been evaluated as response to different salinity and water temperature. Three different salinities (0 ppt, 10 ppt and 20 ppt) in combination with two different water temperatures (26° C and 30° C) have been applicated in this experiment. Fish with average body weight 28.3 ± 2.62 g were used ini this experiment. The result showed that plasma osmolality of nile in freshwater have not different with in 10 ppt (P > 0.05), but plasma osmolality increased at 20 ppt especially at 8,16 and 24 hours after aclimated on 20 ppt. Osmoregulatory capacity has signifi cant heigher (P < .05) on nile in freshwater than in water with salinity 10 ppt and 20 ppt. Body water content and blood hematocrite were not signifi cant between different salinity and water temperature (P >.05). Conclution, osmotic regulation of nile showed signifi cant different between salinity treatment, but blood hematocrite only changed at 8,and16 hours after nile aclimated on water salinity 20 ppt and body water content not changed at both diferent salinity and water temperature.

ANTI-INFLAMMATORY EFFECT OF PORTERWEED (Stachytarpheta Jamaicensis (L.) Vahl) LEAF EXTRACT TO TNF-α EXPRESSION Anastya Eka Kharisma; Hendra Susanto; Muhammad Rifqi Hariri
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

Porterweed (Stachytarpheta jamaicensis (L.) Vahl) contain many fl avonoids compound. It is a potential candidate to supress the inflammatory process through down regulation of TNF-α level as a potent pro-infl ammatory cytokine in chronic infl ammation cases. The aim of this research was to improve the effect of Porterweed leaf extract to the TNF-α level and considered the optimum dosage in wistar rats with chronic infl ammation model. Twenty fi ve rats was divided into negative control group, positive control group, and treatment groups with dosage 50, 100 and 150 mg/kg BW. Data obtained through microscopical histologic observation on aortic organafter immunohistochemistry staining and was analyzed descriptively. The result revealed that Porterweed leaf extract is able to inhibit the increasing of TNF-α level. This phytochemical substance is predicted capable of preventing the oxidation reaction progession ofcyclooxygenase-2 (COX-2) to produce prostaglandins. The decreasing of prostaglandin level causes a negative feedback to TNF-α production and iniciating the declining level of TNF-α. Thus, the Porterweed leaf extract indicates supress chronic infl ammatory process through down regulation of TNF-α and prostaglandin activity in optimum dose 150 mg/kg bw.

THE N-HEXANE FRACTION OF KESUM (Polygonum Minus L.) INDUCE APOPTOSIS THE LUNG EPITHELIAL CELLS OF THE RATUS NOVERGICUS THAT EXPOSED BY BENZOPYRENE Muhamad Agus Wibowo; Basuki Bambang Purnomo; Mohammad Aris Widodo; Aulanni’am Aulanni’am
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

Kesum (Polygonum minus L.) is a popular plant and is often used as additives in many typical dishes in the West Kalimantan. These plants belong to the family Polygonaceae, and potential to use in the prevention and treatment of lung cancer. This research aimed at studying the effect of giving the n-hexane fraction of the kesum to induces of apoptosis in epithelial cells of lung tissueof the animal models that exposed by benzopyrene. research was conducted on three groups of animal models: i) the healthy group, ii) the group treated by benzopyrene 200 mg/kg, and iii) the therapy group by n-hexane fraction 100 mg/kg. The results of the study then carried measurements of lung epithelial cell apoptosis index and measurements of lung ROS levels. The results showed that n-hexane fraction able to reduce of lung ROS levels of the animal models that exposed by benzopyrene with MDA levels respectively are 5.09 ± 0.76 ppm for the healthy group, 8.44 ± 1.89 ppm for the group treated by benzopyrene, and 5.47 ± 1.76 ppm for the therapygroup. The n-hexane fraction also able to induce apoptosis of the lung epithelial cells of the animal models that exposed by benzopyrene with apoptosis index values respectively are 2.61 ± 1.98 for the healthy group, 7.41 ± 2.26 for the group treated by benzopyrene, and 16.31 ± 5.73 for the therapy group.

DETEKSI SENYAWA ISOFLAVON DAIDZEIN DAN GENISTEIN PADA KULTUR IN-VITRO KALUS KEDELAI (Glycine Max Merr.) Tintrim Rahayu
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

The aim of this research is to identify the isofl avon compounds in the in-vitro cultured callus of soybean (Glycine max Merr.). This is an explorative research, in which callus were cultured in the B5 medium supplemented with 2 ppm 2,4 D. The friable callus were found when it was cultured in the solid medium containing 8 g/l agar and 20 g/l sucrose. When the callus and soybean were extracted with ethanol, a yellow colored substance appeared. If further analysis was done with thin layer chromatography (TLC) method employing 0,2 mm thin layer silica gel 60 F254 (DC-Plastikfolien Schicht-dicke), and eluent consisting n-Butanol - HCL 0.1 N (1:1), six light bluecolor nodes appeared under 366 nm UV light. The nodes have the following Rf: 0,14; 0,30; 0,52; 0,63; 0,79 and 0,92 respectively. This TLC result is comparable with the TLC result from soybeans since they have two nodes with the same Rf and color, namely blue color at Rf 0,81 and 0,92 respectively. Further confi rmation using HPLC (High Performance Liquid Chromatography) equipped with UVvis detector and Lichrospher 100RP–18, (10 μm) colom, as well as Hitachi D–2500 Chromato-integrator indicated that those similar two nodes identifi ed in the TLC were either daidzein or genistein. They can be detected by HPLC at 250 nm and 260 nm, when theywere eluated at the 80% metanol. The HPLC quantitative calculation indicated that concentration of daidzein is four time higher as it was compared with the daidzein concentration in the bean. The concentration of daidzein in the callus remained high up to 4–5 weeks after plantation. It’s concentration will decrease when the callus reached 6 weeks after plantation. Genistein as another component of isoflavon is not appear upon callus, while on soybean seeds extracts, both daidzen and genistein are detected.

EFFECT HYDRAULIC AGITATION USING ANAEROBIC BIOFILTER FOR TREATED DOMESTIC WASTEWATER Nur Indradewi Oktavitri; Agoes Soegianto; Trisnadi Widyaleksono C. Putranto; Angsukma P. Dewayanti; Adam L.H. Suryawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

The objective of this study was to investigate the effi ciency the TSS reduction of domestic wastewater by using hydraulic agitation in anaerobic biofi lter. The reactor consists of four columns. Each column has a sampling point. Upfl ow direction was operated in reactor in order to maximize the hydraulic agitation. Gravel was used as fi lter media to support the growth of biofi lm. The reactor treated wastewater of FST Airlangga University canteen which contains 8237.463 mg/l of TSS. Flow used during processing was 0.3 ml/min. Sampling TSS were performed on days 7, 14, 21, and 28. The results showed that the effi ciency of TSS were 64.57%, 76.16%, 94.23% and 98.46% respectively. The average TSS removal was 83.36%.

ANALISIS LAJU TIMBUNAN DAN KOMPOSISI SAMPAH DI PERMUKIMAN PESISIR KENJERAN SURABAYA Nita Citrasari; Nur Indradewi Oktavitri; Nuril A. Aniwindira
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

Solid waste in Coastal Settlement Kenjeran Surabaya is alarming due to of the habit of the peoples to pile until 1 meter on surface coastal land. The habit can impact of environmental pollution to that area. So, the purpose of this research was to know the solid waste generation and the composition of solid waste in Coastal Settlement Kenjeran Surabaya. The methods used was weigth volume analysis. That result of research showed that the solid waste generation was 0.23 kg/person/day with the composition 76.21% of garbage, 10.83% of plastic, 5.33% of paper, 0.82% of glass, 1.21% of wood, 2.27% of fabric, 0.44% of metal, 0.08% of foam, and 2.58% of the others(soil, sand, and gravel). The solid waste generation and the composition of solid waste could be used for the planning of management in Coastal Settlement Kenjeran Surabaya.

SELF ASSEMBLY AND MAGNETISM OF LIVING BIOLOGICAL MOLECULES Sutiman Bambang Sumitro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 2 (2012): June 2012
Publisher : The East Java Biological Society

Biological molecules are essentially nano size structure. All of them are complex structure with specifi c function dedicated to perform normal ordered organizational system. The forces for their work are non-covalent interactions; include spontaneous folding of proteins, DNA, RNA and other bio-macromolecules, ligand-receptors interactions, assembly-disassembly of macromolecule, andtransportation or movement of many other nano size sub cellular components. The non-covalent interactions are weak bonds system that is low energetic chemical and physical forces. The energetic forces are mainly atomic forces such as electromagnetic force emergence from electron spinning and transitions at every atom of the complex macromolecular structure. The energy will work along with different level of energy, and atomic positioning within macromolecules. This paper review and discuss the role of magnetism on molecular working process as part of thermodynamically open systems to develop order, which is constantly receiving, transforming and dissipating energy, can and do continually exhibit self assembly and organization, along with the self repairing, and perpetuation.

PENGARUH MANIPULASI MEDIA TERHADAP KANDUNGAN ALKALOID VINKRISTINA KALUS DAUN Catharanthus roseus(L.) G.Don Y. Sri Wulan Manuhara
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 1 (1995): June 1995
Publisher : The East Java Biological Society

The effect of the manipulation of the media on the vincristine alkaloid content in the callus of Catharanthus roseus (L.) G.Don were studied. This work was done as an effort for gaining the vincristine alkaloid through tissue culture which was expected to obtain a larger amount of the alkaloid. Tissue culture of C. roseus was initiated from leaf explants on growth medium (MS supplemented with 1 mg/l 2,4-D and 1 mg/l BAP). after seven weeks incubation, with only subculturing on the same medium, the proliferating calli were subculture on a production medium (MS supplemented with 1mg/l IAA and 1 mg/l BAP) which were containing different additional concentration of sucrose, BAP, tryptophan, and concentration of 50% basic medium of MS from the standard. Eleven weeks-old calli were harvested from each treatment and dried for chemical analysed by thin layer chromatography on silica gel GF 254 using chloroform-aceton-triethylamine as mobile phase. Rf value and uv spectra were used to identify vincristine, and concentration of vincristine alkaloid was determine by preparative thin layer chromatography with methanol solvent and measured by uv-vis spectrophotometer at 233 nm. The maximum content of vincristine alkaloid was obtained from callus, which was grown on the MS standard with an addition of 40 g/l sucrose or 4 mg/l BAP or and also 50% of the MS standard medium. Tryptophan addition a precursor could not induce the alkaloid vincristine forming.

ISOLASI DAN KARAKTERISASI MUTAN sal4 DI RAGI Saccharomyces cereviceae Ni Nyoman Tri Puspaningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 1 (1995): June 1995
Publisher : The East Java Biological Society

Recently, genetics manipulation in yeast Saccharomyces cereviceae have much been done. It because yeast can be used as a host cell alternative in the foreign protein expression, therefore information about fidelity from yeast should be studied. Preliminary study showed that SAL 4 gene has assumed to has a role in translation fidelity control and/or termination factor. To study the gene function, mutation in yeast BSC483/1a has been done by Ethylmethane sulphonate. Mutants wished are mutated at sal 4 locus and have characteristic of both allosuppressor and omnipotent suppressor. Phenotype of allosuppresor mutants were indicated by white colour consistency in YPD and Y8 medium, temperature sensitivity, paromomycin sensitivity and growth rate. Quantitatively, effectiveness as omnipotent suppressor has been done by using gene fusion between PGK and B-galaktosidase. The result showed that BSC483/1a strain could be mutated by Ethylmethane sulphonate 1% and produced eight allosuppressor mutants. Two of them (Number 8 and 10) have characteristic of temperature sensitivity, and the two others (Number 1 and 13) were mutated at sal 4 gene locus. Characterize of sal 4 mutants (1 and 13) didn't show temperature sensitive and have growth rate relatively more slowly than the wild type. Mutant (number 13) could suppress nonsense mutation (realthrough) at termination codon UAG with B-galaktosidase activity as amount 2.70 unit/ml.

Page 13 of 51 | Total Record : 507

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Home Page

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Contact Name Sutiman Bambang Sumitro

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Sutiman Bambang Sumitro

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berkalahayati@yahoo.com

Phone
+62341570631

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wulidanisa@berkalahayati.org

Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia

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INDONESIA