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Berkala Penelitian Hayati

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Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur

ISSN : 08526834 EISSN : 2337389X DOI : https://doi.org/10.23869/bphjbr

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 507 Documents

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KAJIAN KOMPOSISI SERANGGA POLINATOR PADA TUMBUHAN PENUTUP TANAH DI PONCOKUSUMO – MALANG Budi Purwantiningsih; Amin Setyo Leksono; Bagyo Yanuwiadi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 2 (2012): June 2012
Publisher : The East Java Biological Society

The use of pesticides on agricultural land has a negative impact on health, economic, and environment. The using of variety of ground cover plants as refugia area are expected to be an alternative habitat for pollinators insect in plants: Cyperus rotundus L., Bidens pilosa L., Commelina difussa L., Capsicum frutescens L., and Ageratum conyzoides L. Observations made by "visual control". This reseacch aims to know the composition of pollinators insect visit of ground cover plants and analyze their patterns. Analysis of the data structure of insect communities in the spring and the fruit season obtained from INP and diversity (Shannon-Winner Index). Parameters are compared to diversity and abundance with ANOVA test, composition using Morisita Index. The results show that the abundance of insect pollinators of spring higher at 7 families (31%) with H '= 1.698 instead of the fruit season is 6 families (25%) with H' = 1.600. Abundance and diversity of pollinators insect in the spring and fruit season with a significance of P < 0.05. Morisita similarity index of 82%. Analysis of environmental factors of temperature, humidity, and light intensity on the abundance of insect pollinators found positive correlation with R-square 87.5%.

EFEKTIVITAS KURKUMIN SEBAGAI ANTIOKSIDAN DAN INHIBITOR MELANIN PADA KULTUR SEL B16-F1 Sugiharto; Arbakariya Ariff; Syahida Ahmad; Muhajir Hamid
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 2 (2012): June 2012
Publisher : The East Java Biological Society

Melanin inhibitors have become increasingly important ingredients in medication and cosmetics for the prevention of hyperpigmentation. In the last few years, a huge number of natural herbal extracts have been tested as inhibitors of melanin synthesis and some of these effects are related to the antioxidant properties. The objectives of this study were to determine of curcumin properties as antioxidant activity and melanin inhibitors. In this study, our data indicated that antioxidant assay with DPPH showed IC50 was 16,05 μg/ml. In the absence of α-MSH (α-Melanocyte Stimulating Hormone), melanin content assay in cell B16-F1 indicated that the highest activity of curcumin to reduce melanin content of 45,67% at 25 μg/ml. Meanwhile, in the presence of α-MSH at the same concentration indicated that the highest activity was 53,87%. Based on the data, curcumin has potential properties as antioxidant activity and melanin inhibitor.

AKTIVITAS POLISAKARIDA KRESTIN DARI EKSTRAK Coriolus versicolor TERHADAP PENINGKATAN ANTIBODI Mus musculus AKIBAT PAPARAN Mycobacterium tuberculosis Sri Puji Astuti Wahyuningsih; Amalia Nur Mashita; Dwi Winarni
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 2 (2012): June 2012
Publisher : The East Java Biological Society

This research aimed to know the bioactivity of polysaccharide krestin (PSK) with a different timing on formation of mice antibody exposed by Mycobacterium tuberculosis. Mycobacterium tuberculosis was used as infection agent that had an abilitiy to be imunogenic and to lead immune system responses decreased. Polysaccharides krestin was used as immunopotentiator which had a role to activate macrophage and stimulate B cell in order to produce antibody. This research involved 30 adult female mice of Mus musculus strain Balb/C in the age of 8–10 weeks and in the weight between of 30–40 g. Mice were divided into six groups, as follows: group K as a control, was added only aquades; group K+ as positive control, was added only PSK; group K- as negative control, was exposed by M. tuberculosis only; group P1 was added PSK before being exposed by M. tuberculosis; group P2 was added PSK after being exposed by M. tuberculosis; group P3 was added PSK before and after being exposed by M. tuberculosis. Polysaccharides krestin was added by gavage with single dose of 500 μg and M. tuberculosis was exposed intraperitoneally with concentration of 5x108 bactery per ml. The bioactivity of PSK was observed by formation antibody using indirect ELISA test. The results of research were the average of OD value of K group was 0,590 ± 0,042, K+ group was 0.641 ± 0.025, K- group was 1.044 ± 0.054, P1 group was 1.032 ± 0.125, P2 group was 1.127 ± 0.042, P3 group was 1.230 ± 0.097. The conclusion of this research showed that the adding of PSK increased mice formation antibody. The time of adding before and after being exposed M. tuberculosis was the most potential to raise antibody production on mice.

BEBERAPA SIFAT FISIKA KIMIA TANAH YANG BERPENGARUH TERHADAP MODEL KECEPATAN INFILTRASI PADA TEGAKAN MAHONI, JABON, DAN TREMBESI DI KEBUN RAYA PURWODADI Agung Sri Darmayanti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 2 (2012): June 2012
Publisher : The East Java Biological Society

The research was conducted at the Purwodadi Botanical Gardens in three areas covered by the dominant vegetation Mahoni (Swietenia macrophylla), Jabon (Anthocephalus cadamba) and trembesi (Samanea saman) in January to May 2012. The purpose of this study was to obtain a model infiltration rate (cm/h) that was influed by physical and chemical properties of the soil that exist under the vegetation. Physical and chemicalparameters of soil chemical properties is observed macropore (%), organic matter (%), percentage of the fraction silt (%), sand(%), and clay(%), and bulk density (BD) (g/cm3). The six factors are thought to differ indirectly because of the specification and morphology of the dominant plant species shelter. Data infiltration was collected by using the ring infiltrometer, while macroporosity tested with metylen blue method, the properties of the other physical chemical factortested by laboratory of Department of Soil, Brawijaya University. The model derived from the analysis of the regression equation using SPSS software version 17.0. The study produced three different models of infiltration rate on each plot different, they were Y = - 15,8 + 17,3 X1 - 1,09 X2 + 1,53 X3 + 0,001 X4 - 21,3 X6 (Mahoni's plot);Y = - 108 + 53,0 X1 - 0,68 X2 + 5,27 X3 - 0,470 X4 + 59,7 X6 (Jabon's plot); Y = - 20,1 + 17,4 X1 - 1,06 X2 + 1,57 X3 + 0,082 X4 - 21,6 X6 (Trembesi's plot). Y= Infiltration rate (cm/h), X1= macropore (%), X2= organic matter (%), percentage of the fraction silt (%) (X3), sand (%) (X4), and X6= bulk density (g/cm3). In the equation not found factor X5 (clay fraction) helped form a model because of multicollinearity analysis obtained by assuming that the clay fraction still contains a linear relationship (correlated) with other variables.

STUDI ANATOMI DAUN DAN BATANG Maesopsis eminii (Rhamnaceae) Eka Fatmawati Tihurua; Sunaryo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 2 (2012): June 2012
Publisher : The East Java Biological Society

Maesopsis eminii is one of alien and invasive plant in Indonesia and planted in Resort Bodogol, Gunung Gede Pangrango National Park. The results showed M. eminii has dorsiventral and hypostomatic leaf type with anomocytic stomata, drusse type of CaCO3 crystal, multicellular and uniseriate trichomes in the upper and lower surface. While its stem has variation in its ray initial layer depend on its trunk circumference.

PERKEMBANGAN KULTUR DAUN Aglaonema sp. var Siam Pearl, Aglaonema sp. var. Lady Valentin dan Aglaonema sp. var. Lipstik DENGAN PERLAKUAN ZAT PENGATUR TUMBUH IAA DAN BAP Ika Sukma Dewi; Dwi Kusuma Wahyuni; Hery Purnobasuki
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 2 (2012): June 2012
Publisher : The East Java Biological Society

The aim of this research was to know the effect of 3-indole-acetic acid (IAA) and 6-bensilaminopurine (BAP) and optimum concentration of IAA+BAP toward growth and development of the leaf culture of Aglaonema sp. var. Siam Pearl, Aglaonema sp. var. Lady Valentine, and Aglaonema sp. var. Lipstik. The explants were taken from the leaf. The explants were cultured in solid MS medium with addition various concentration of IAA 0,5 mg/l, 2 mg/l, 3 mg/l, 5 mg/l, and 8 mg/l and BAP 5 mg/l. Observation had been conducted for 16 weeks to know the response the development of explants leaves such as the periphery of explants coloured of brown, leaves explants of arch shaped, the colour of explants to change for pale, and to form callus. The data were include of the number of explants which able to form callus, time of callus formation, the colour and texture callus, and stage of development explants were analyzed descriptively. The result of 16 weeks observation showed that the addition of IAA and BAP on the leaves explants to give the different effects. Aglaonema sp. var. Lipstik can form callus by treatment of 5 mg/l IAA:5mg/l BAP, and 8 mg/l IAA:5 mg BAP. The colour of callus are clear, the shape of globular, with friable textures.

THE PROFILE OF BAMA BEACH BASED ON THE SUBSTRATE, THE PRESENCE OF SEAGRASS, CORAL LIFEFORM, AND ECHINODERMATA Noorafebrianie Minarputri; Noer Moehammadi; Bambang Irawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 2 (2012): June 2012
Publisher : The East Java Biological Society

Bama Beach is one of the most visited beaches in Baluran National Park, Situbondo. However, this beach does not have information of beach profile which is very important for managing coastal and marine resources. The aims of this research are to make beach profiles based on substrate, presence of seagrass, coral lifeforms, and Echinodermata. This research’s method is qualdrate transect. Five transects were made, roll meter pulled from the highest tides zone to the reef crest. The distance between each transect around 35–40 m. Plots 1x1 m2 were placed along each transect with 8-15 m gap between each plot. Geographical coordinates were noted for each plot so the results are data in those plots. The results are the dominant substrate in Bama Beach is sand and always mixed with gravel. Boulders specifically found in the highest tides zone and close to the reef crest. Seagrass can be found almost in all plots, about 10 to 250 m from the highest tides zone. The dominant coral lifeform on reef flat is coral submassive; then approaching the reef crest, the coral massive is the dominant one. At Bama Beach, was found 5 classes and 9 orders of Echinodermata. Asteroidea with order Valvatida and Spinulosida; Ophiuroidea with order Ophiurida; Echinoidea with order Camarodonta, Diadematoida, Stomopneustoida; Holothuroidea with order Aspidochirotida and Apodida; and Crinoidea with order Comatulida. Those orders live in all zones, which are sandy zone, seagrass zone, and coral reef zone.

PENENTUAN JENIS KELAMIN BULU BABI Tripneustes gratilla SECARA MORFOLOGI Abdul Hamid A. Toha; Agung Pramana; Sutiman B. Sumitro; Luchman Hakim; Widodo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 2 (2012): June 2012
Publisher : The East Java Biological Society

Determining the sex of T. gratilla was difficult and generally determined directly by the presence, length and shape as well as size gonopore genital papilla. We tried to determine of the gender 17 individual of T. gratilla based on morphological approach. Injection of KCl and cleavage of the whole individual is also carried out to observe the color of the gonads as a reference for determining the sex. We found 6 samples of female and 11 male samples based on gonad color. The results of morphological measurements showed that all parameters used have not been able to determine the sex of T. gratilla, either singly or in combination parameters. The number of samples need to be added till at least 30 individual each sex. This is consistent with logistic regression 3, where the P-value is less than the P-value logistic regression 1 and 2, which enables different results if the sample size is enlarged.

Amplifikasi Gen CRY1 dan Analisis Genom Isolat Bacillus thuringiensis Lokal Dwi Suryanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009
Publisher : The East Java Biological Society

A study on amplification of Cry1 gene and genome analysis of local isolate of Bacillus thuringiensis TU1 has been done. Amplification was done for cryI gene by PCR-technique. Genome analysis was performed using pulsed-field gel electrophoresis. Insecticidal specificity assay of the isolate to larvae was done in larvae of Heliothis armigera, Plutella xylostella, Aedes aegypti, and Culex sp. Isolate of commercial strain, B. thuringiensis var kurstaki strain HD-7, was used as control for amplification of cryI gene, genome analysis, and insecticidal specificity assay. The result showed that insecticidal specificity assay of the isolates of both TU1 and commercial have similar spectrum of toxicity to the larvae. Amplification of cry1 gene resulted to a fragment of approximately 550 bp in both TU1 and commercial. Genome profiles of TU1 and commercial strain were similar.

STATUS KUALITAS SUNGAI MUSI BAGIAN HILIR DITINJAU DARI KOMUNITAS FITOPLANKTON Hilda Zulkifli; Husnah; Moh. Rasyid Ridho; Suhodo Juanda
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009
Publisher : The East Java Biological Society

Musi river is the main source of water for society and industry on the downstream area, and also becomes the waste disposal area for domestic/industry. The objective of this study was to evaluate the status of Musi river on the downstream area based on phytoplankton community (from the Pulokerto district to downstream of musi river on Tanjung Buyut Village). The sample of water surface and plankton were taken compositely on 18 stations. The water quality analysis is held on LIPI Bogor, while the identification of microscopic community of phytoplankton is held on BRPPU Mariana. The result of research showed that phytoplankton community on observed locations comprises of Chlorophyta with 18 genus (45.34%), followed by Cyanophyta with 7 genus (26.09%), Baccillariophyta with 14 genus (25.81%) and Euglenophyta with 2 genus (2.17%). The Oscillatoria (Cyanophyta) is found on most of observed locations. Based on the examination of saprobic status, Pulokerto station is categorized as “moderate polluted” or (ά/ß –mesosaprobik dan ß/ά –mesosaprobik), while closer to downstream of Musi river is categorized as “low polluted” or (oligo/ß –mesosaprobic).

Page 18 of 51 | Total Record : 507

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Home Page

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Contact Name Sutiman Bambang Sumitro

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Editorial Address Jalan Surakarta No. 5 Malang, Indonesia

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Sutiman Bambang Sumitro

Contact Email
berkalahayati@yahoo.com

Phone
+62341570631

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wulidanisa@berkalahayati.org

Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia

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INDONESIA