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Berkala Penelitian Hayati

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Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur

ISSN : 08526834 EISSN : 2337389X DOI : https://doi.org/10.23869/bphjbr

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 507 Documents

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Phytochemical study on the flower of Alstonia macrophylla Wall. ex G.Don (Apocynaceae) from Sumbawa Island, Indonesia Putri Sri Andila; Tri Warseno; I Putu Agus Hendra Wibawa; I Gede Tirta
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 2 (2019): June 2019
Publisher : The East Java Biological Society

Alstonia macrophylla Wall. ex G.Don is a native plant to South East Asia, belonging to Alstonia genus. This species has been reported to have numerous natural chemical compound which perfom multiple pharmacological and biological activities. The aim of this study was to investigated the phytochemical properties of the acetone extract of the flower of lstonia macrophylla Wall. ex G.Don. This is very interesting because phytochemical properties of its flower had been never reported yet. Alstonia macrophylla was harvested from the Natural Forest of Punik, Batu Dulang Village, Batulanteh Subdistrict, Sumbawa Regency, West Nusa Tenggara, Indonesia on Mei 2015. Acetone flower extract of A. macrophylla was analyzed with a GC-MS method to determine the chemical components. Result of GC–MS chromatogram revealed that there were 30 identified components in this extract. The major compounds were Cycloartenol acetate (17.11 %); 5H-1-Pyrindine (12.44 %); Lupeyl acetate (10.12%); Oleic acid (6.08 %); Benzenesulfonic acid, 4-hydroxy- (4.25 %); p-n-Amylphenol (4.23 %); and 4-Methylindole (4.22 %). Here, We reported the first study of phytochemical properties of A. macrophylla. This study help to understand further detail the potential of bioactive compound of A. macrophylla.

Combination of culture and dyeing conditions on cloth color dyed with mixed fungi dyes Suciatmih Suciatmih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

This study aimed to examine the dyes production of mixed Aspergillus and Paecilomyces on different culture conditions and evaluate the combination between culture and dyeing conditions on cloth color by the fungal dyes. The different media consist of mineral salt glucose (MSG) medium and mineral salt sucrose (MSS) medium were evaluated. Additionally, different culture conditions such as C and N sources, incubation temperature, and pH of the media were evaluated together with variation of dyeing conditions including pH and mordants. The result showed that different culture conditions on media and the combination of those with dyeing conditions produce distinction colors on the fabric. The variation of dyed colors on the fabric was related to the dyes concentration produced by combination treatment of culture condition and dyeing pH. However, the effect was not observed on the combination between culture condition and mordants. Combination of culture and dyeing conditions on cloth color dyed with mixed fungi dyes generated various cloth colors, adding a color variation on textile dyeing.

In vitro radiosensitivity of lymphocytes from high-background radiation ar-ea inhabitants in Indonesia: a pilot study using the G0 micronucleus assay Dwi Ramadhani; Sofiati Purnami; Siti Nurhayati; Eko Pudjadi; Mukh Syaifudin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 1 (2018): December 2018
Publisher : The East Java Biological Society

The Mamuju area has recorded a higher than average natural radiation dose compared to other regions in Indonesia. It is possible that inhabitants of Mamuju developed the radioadaptive response (RAR). To prove that the RAR was developed in Mamuju inhabitants, evaluation of lymphocytic radiosensitivity using the G0 micronucleus (MN) assay was conducted herein. The MN frequencies in peripheral blood lymphocytes before and after exposed to 1.5 Gy radiation at a dose rate of 0.38 Gy/min were assessed from 11 samples of Botteng Village, Mamuju inhabitants and control groups. These treated cells were then cultured and exposed to cytochalasin-B to arrest cells during the cytokinesis stage. The mean number of MN induced by radiation per 1000 binucleated cells (BNC) was evaluated for both Mamuju inhabitants and control groups. The lymphocytic radiosensitivity was calculated using standard deviation (SD) of the mean value (MV) of the number of MN induced by radiation found in the control group. Based on the SD and MV values, individual lymphocytes were classified as ‘‘resistant’’, ‘‘sensitive’’, or ‘‘normal’’ if the mean of MN induced by radiation was ≤ MV – (2×SD), ≥ MV + (2×SD) or in between, respectively. The results showed that lymphocytes from six samples in Botteng Village inhabitants were categorized as resistant and five samples were normal. In contrast, all samples in the control group were demonstrated to have normal sensitivities. The study results also revealed that lymphocytic radiosensitivity was not correlated with age and gender, which was probably because of the small sample size used in this study. Further investigation with a larger sample number and higher radiation dose should be conducted to validate our findings.

Antibacterial activity of water and ethanol extract of Allium sativum, Curcuma mangga, and Acorus calamus combination Bayyinatul Muchtaromah; Mujahidin Ahmad; Romaidi Romaidi; Lina Ainun Nazilah; Nayla Alin Naja
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 1 (2018): December 2018
Publisher : The East Java Biological Society

Madura has been known as an Indonesian tribe that usually used many recipes of traditional medicine in their daily life. One of the medicinal herbs to increase female fertility is the herbal "Subur Kandungan", which consists of garlic (Allium sativum), temu mangga (Curcuma mangga), jeringau (Acorus calamus). The purpose of this study was to determine the phytochemical content and antibacterial activity of A. sativum, C. mangga and A. calamus combination in water and ethanol solvent against Streptococcus aureus and Escherichia coli. There were three kinds of combinations with different composition ie first combination/C1 (36:36:28); second combination/C2 (40:30:30); third combination/C3 (35:40:45). Clindamycin was as a positive control. The phytochemical screening detected triterpenoids in both extracts, while alkaloids, flavonoids only in ethanol extracts. The highest inhibitory zone of both extracts to S. aureus and E. coli were discovered in C3 as strong and moderate inhibition. The most effective MIC value of water extract against S. aureus was found on C3 (9.76 x1010) while ethanol extract was obtained by C1 (5.9x108) at concentrations of 0.39% and MBC at 0.78%. The best MIC value of water extract against E. coli was found on C1 (1.08x1010) at a concentration of 25% and MBC at 50%, whereas ethanol extract was got on C3 (9.7x109) at 0.39% & MBC at 0.78%. It could be concluded that “Subur kandungan” herb recipes could be used as antibacterial drugs, which third combination/C3 in the ethanol solvent is the best treatment compare with others.

Seven species of Labidocera (Copepoda, Calanoida, Pontellidae) from off Kenjeran, Surabaya, Indonesia Dewi Citra Murniati; Mulyadi Mulyadi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 1 (2018): December 2018
Publisher : The East Java Biological Society

During taxonomic studies on the pelagic copepods of the Indonesian waters, seven species of Labidocera were identified from off Kenjeran, Surabaya during 1994-2012. Four of these species, L. acuta (Dana, 1849), L. kroyeri (Brady, 1883), L. laevidentata ((Brady, 1883), and L. minuta (Giesbrecht, 1889) have been recorded previously, L. javaensis (Mulyadi, 1997) has been described as new species, and two species, L. bengalensis (Krishnaswamy, 1952) and L. sinilobata (Shen & Lee, 1963) were new records for Indonesian waters. Labidocera bengalensis and L. minuta belong to the L. minuta-group, L. kröyeri to the L. kröyeri-group, L. javaensis to the L.pectinata-group, L. sinilobata to the L. detruncata-group, while L. acuta and L. laevidentata to the unassigned-group. The number of species were highest in June 1994 and June 1998, each with 5 recorded species. The number of species were decreased, 3 species in November 2000, 1 species in October 2010 and 2 species in July 2012. Labidocera sinilobata has been found for all times sampling, L. javaensis for four times, L. acuta and L. kroyeri are found twice, and L. bengalensis L. laevidentata and L. minuta are found for one time sampling. Descriptions, measurements and figures are given for these species, along with a review of their distribution over the world’s oceans, and with taxonomic remarks, ecological notes, their species groups, and restricted synonyms.

Screening of plant growth promoting rhizobacteria (PGPR) to promote growth of soybean Sri Widawati; Sulasih Sulasih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 1 (2018): December 2018
Publisher : The East Java Biological Society

Rhizobacteria is an essential component for the maintenance of soil fertility and plant growth. The objective of the study was to characterize plant growth promoting rhizobacteria (PGPR) activity such as phosphate solubilization, nitrogen-fixing, IAA production, Acc-deaminase and cellulase activity isolated from the rhizosphere and nodules of soybean and evaluate their effectiveness in stimulating soybean seedlings growth. Greenhouse assay was conducted by completely randomized design with three replications per treatment and two controls (K1-control without inoculation of bacteria and Nitrogen, K2-control inoculated with Bradyrhizobium japonicum. Pot experiments were conducted using sterile sand and supplemented with water and Muller solution, periodically. The results showed that nine rhizobacteria isolates were efficacious as potential PGPR. Greenhouse assay results showed that the highest vegetative growth yield was obtained in soybean inoculated with SWNC1 isolate, it was characterized as Bradyrhizobium spp. which gave the total nodules of 29.67; fresh weight of nodules of 0.61 g; nodules dry weight of 0.09 g; number of flowers of 23; plants dry weight of 5.57 g and plant height of 46 cm.

The automatic transmission motorcycle ultrafine particles PM0.1 effects in the alveolar enlargement, depleted of septum alveolus, and lung inflammation Arinto Yudi Ponco Wardoyo; Unggul Pujung Juswono; Johan A.E. Noor
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 1 (2018): December 2018
Publisher : The East Java Biological Society

Motorcycles smoke have been responsible inducing lungs related to diseases for a long time. This study was aimed to reveal the effect of the repeated exposure automatic transmission motorcycle smoke in the development of lungs damage in the wistar mice by observing the alveolar, the septum alveolar, and the tissues inflammation. Threes groups of the wistar mice had been labelled as control, C1, and C2 and keep in 30 x 20 x 20 cm3 chamber. The C1 group was introduced by the motorcycle smoke for 60 second and C2 for 100 seconds. The smoke was flowed into a chamber by using an air pump with the rate of 2 m/s. The smoke was exposed repeatedly to the mice as long as 10 sequence days, and the lung was observed for every 2 day. The alveolar size was observed by measuring the line core length. The septum alveolar was indicated by the depleted septum alveolus, and the inflammation was identified by the morphological way. All of the result were statistically analysed. The alveolar line core grew longer for the C1 and C2 group of 11±6 μm and 20±2 μm compared to the control. The Depleted Septum Alveolar increased up to 77% for the C1 group and 79% for the C2 group. The tissue inflammations were found in every sample. The R2 from statistical approach showed the strong correlation between the lung damage and the repeated exposures. The lung damage in terms of alveolar size enlargement, the increase of damage septum alveolus, and tissues inflammation were related to repeated exposure of motorcycle smoke.

The orbicules and allergenic protein of African tulip tree (Spathodea campanulata P.Beauv.): a roadside ornamental plant in Malang, Indonesia Janis Damaiyani; Yekti Asih Purwestri; Issirep Sumardi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 1 (2018): December 2018
Publisher : The East Java Biological Society

Orbicules or ubisch bodies are capable carrying specific pollen antigens which responsible for presenting allergenic activity in the atmosphere. Currently, there is no report on the orbicules and allergenic protein in African tulip tree. Thus, this research was aimed to investigate the presence of orbicules and allergenic protein in African tulip tree and to determine their potential as allergen source. The ultrastructure of orbicules were detected using Transmission Electron Microscope (TEM) and Scanning Electron Microscopy (SEM). Allergenic proteins were analyzed based on the molecular weight using SDS-PAGE. The result showed that he orbicules were present in the tapetal cells. Furthermore, the pro-orbicules were released by tapetal cells and fill the loculus before anthesis. The SDS-PAGE analysis showing several proteins with a molecular weight of 10-70 kD were detected in African tulip tree and cauld be potential as source for allergen. Protein bands with a molecular weight 55 kDa, 48 kDa, 39 kDa, and 27 kDa appear with the darkest, clearest and thickest expression of genes compared to other bands. In other species, the protein bands with molecular weight 55 kDa, 48 kDa and 27 kDa were detected as allergen. This results indicated African tulip tree could be potential for pollen allergen from ultrastructure and allergenic protein observation.

Diversity, activity analysis and effectivity of rhizobacteria in plants rhizosphere on the growth of Arachis hypogaea L plants Sri Purwaningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 1 (2018): December 2018
Publisher : The East Java Biological Society

This research was conducted to observe the diversity, activity analysis and effectivity of rhizobacteria in plant rhizosphere on the growth of Arachis hypogaea L plants. The soil samples were collected from rhizosphere soil from different plants at Suro Muncang village and Bukit Peninjauan village. The rhizobacteria was isolated in YEMA media to test the qualitative activity including nitrogen, indole acetic acid (IAA) fixation, and siderophores. An isolate used in this research were 1 RB, 2 RB, 8 RB, 9 RB, 10 RB, 11 RB, 16 RB, 17 RB, 19 RB, 20 RB, 21 RB. Experimental plants were harvested after 45 days. The parameters used were the dry weight of shoots, roots, nodules root, total plants, number of nodules, and symbiotic capacity. The research design was conducted by Completely Randomized Design with three replications per treatment. The results showed that the population of rhizobacteria ranged between 13-51 x 105 CFU/g soil. Rhizobacterial isolates showed different level of plant growth promotion, which is the isolate 19 RB (isolate from Leucaena leucacephala plant) has a higher influence to the growth of A. hypogaea L.

Regeneration and histological study of somatic embryogenesis of sugarcane (Saccharum officinarum L.) cultivar PS 864 Fitri Damayanti; Suharsono Suharsono; Aris Tjahjoleksono; Ika Mariska
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 1 (2018): December 2018
Publisher : The East Java Biological Society

The process affecting somatic embryo formation in sugarcane is very specific for each genotype, so the determination of the best somatic embryo regeneration medium in sugarcane clones is necessary. The objective of this study was to determine the concentration of Benzine Amino Purine (BAP) and Kinetin for somatic embryo maturation and to observe the maturation stage for somatic embryo of sugarcane cultivar PS 864. Maturation of the nodular callus was conducted by addition of Kinetin (0, 1, 3, and 5 mg/L) and BAP (0 and 5 mg/L) in solid and liquid medium. The medium was optimized using glutamine. The medium for somatic embryo germination used full and half strength Murashige and Skoog medium (MS) supplemented with 20 g/L sucrose and amino acid or growth regulator (BAP and NAA). Nodular callus well grew in solid medium, whereas callus become browning and not existence of somatic embryo maturation in liquid medium. The highest number of globular embryos (38 embryos) was produced from MS medium supplemented with 1 or 3 mg/L Kinetin combined with 5 mg/L BAP. The highest number of scutellum (21 embryos) and coleoptile (19 embryos) resulted from 3 mg/L Kinetin. The MS medium with full strength was added with 100 mg/L glutamine that was the best germination medium. This medium resulted the highest percentage of somatic embryo (73.29%) forming bipolar structure, and the largest number of leaves (4.58). Histological analysis showed that somatic embryos of sugarcane emerged from many cells through the budding process and also initiated from one cell.

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Contact Name
Sutiman Bambang Sumitro

Contact Email
berkalahayati@yahoo.com

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+62341570631

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wulidanisa@berkalahayati.org

Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia

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Abstract

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Sutiman Bambang Sumitro

Contact Email berkalahayati@yahoo.com

Phone +62341570631

Journal Mail Official wulidanisa@berkalahayati.org

Editorial Address Jalan Surakarta No. 5 Malang, Indonesia

Location Unknown, Unknown INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Sutiman Bambang Sumitro

Contact Email
berkalahayati@yahoo.com

Phone
+62341570631

Journal Mail Official
wulidanisa@berkalahayati.org

Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia

Location
Unknown,

Unknown

INDONESIA