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Berkala Penelitian Hayati

jurnal
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Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur

ISSN : 08526834 EISSN : 2337389X DOI : https://doi.org/10.23869/bphjbr

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 507 Documents

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Biochemical compounds and sub-chronic toxicity test of Chlorella sp. and Spirulina sp. isolated from Glagah Coastal Water Slamet Widiyanto; Mulyati Sarto; Laksmindra Fitria; Rahadian Yudo; Eko Agus Suyono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 1 (2018): December 2018
Publisher : The East Java Biological Society

Microalgae are microscopic photosynthetic organisms found in marine and freshwater environments. This organism is a eukaryotic group of polyphyletic and very diverse. This study investigated the biochemical composition and sub-chronic test of Chlorella Sp. and Spirulina Sp. isolated from Glagah coastal water (Yogyakarta, Indonesia) as a preclinical study to create potential nutritional supplement for human health. Microalgae culture was analyzed for biochemical composition using several methods: AAS for metal analysis (Pb, Cu, Fe, Mg, Zn, and Hg), GC-MS and UV spectrophotometer for fatty acid profiles analysis (lenolenic acid, eicosatrienoic acid, arachidonic acid, linoleic acid, oleic acid, eicosenoic acid, EPA, DHA, omega 6 and 9), Alkaloids, Steroids, Tanin and Saponin. The next step is to do a sub-chronic test of microalgae biomass using experimental animals. The result shows that Spirulina sp. and Chlorella sp. contain 16 and 10 different biochemical compounds respectively. Furthermore, organic nutritional properties in both of microalgae are relatively high with a rich biochemical profile of Fe and nutritious poly-unsaturated fats (such as linolenic acid, arachidonic acid , omega 6, and 9). Regarding sub-chronic toxicity test, it was clear that Spirulina and Chlorella did not affect the blood profile. These findings suggest that both of Glagah strain microalgae are considered to have great potential as a multi-nutritional human health supplement.

Isolation and characterization of LEAFY homologous gene partial sequences from Dendrobium capra J.J. Smith Endemic Orchid of Java Island Muhammad Dylan Lawrie; Ari Indrianto; Aziz Purwantoro; Endang Semiarti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 2 (2018): June 2018
Publisher : The East Java Biological Society

Dendrobium capra is an endemic orchid species on the island of Java, Indonesia, which blooms only once a year, usually in February. In the flowering process, LEAFY (LFY) gene and its homologs are considered as key regulators in flowering of higher plants, including orchids. To understand floral regulatory mechanisms in orchids, information on the flowering gene structure needs to be explored. Partial sequences of LFY homologue of D. capra has been isolated and consists of 220 bp encoding 72 amino acid sequences. Bioinformatic analysis using Blastp search showed high similarity between the LFY genes of D. capra with other orchid LFY genes and has adjacent evolutionary relationship among orchid’sLFY. Amino acid sequence alignment result showed similar characteristic with other orchid’s LFY with conserved motif leucine-rich repeat and DDPRRRLLLLSPDQHN amino acid motif.

In situ study on camouflage behavior of Abdopus aculeatus (Octopodidae) (D’Orbigny, 1834) on the marine water of Panjang island, Buton, Southeast Sulawesi Jeni Jeni; Dwi Listyorini; Endang Suarsini
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 2 (2018): June 2018
Publisher : The East Java Biological Society

Abdopus aculeatus has good camouflage capabilities in nature. The objective of this study is to obtain information on the camouflage behavior of Abdopus aculeatus in nature (in situ). We used an explorative description to observe the camouflage behavior of 24 octopus samples at a number of points on the Panjang Island, Buton Regency. Abdopus aculeatus was identified molecularly using a mtDNA COI blast to determine the target species of observation. Any of the camouflage behavior found was documented and analyzed descriptively. Generally, there are four forms of observations for camouflage behavior; matching body color to wide array of natural background, immersing itself in the sand, resembling a specific object, and being part of the objects surrounding. The various camouflages patterns obtained show a process of defense evolution in minimizing the detection of predators or octopus prey.

In vitro culture technique of Camellia sinensis L for epicatechin production with phosphor inducer Sutini Sutini; Widiwurjani Widiwurjani; Nora Augustien; Hadi Suhardjono; Guniarti Guniarti; Djoko Agus Purwanto; Wirdhatul Muslihatin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

In several ASEAN countries, in vitro culture techniques are one of the cultivation technologies that applied in sustainable agriculture. This technology has been performed in response to obtain the secondary metabolite of epicatechin from Camellia sinensis L. However, The yield of epicatechin is remaining low than in the field production. To overcome this problem, phosphor inducers is desirable to be supplemented in the study. The young leaf shoots of C. sinensis L was sliced on a culture-filled bottle, the sliced part was re-cultivated in the same medium by supplementing of 2,4-D growth regulators, then the medium was optimized by phosphor inducer with range of concentartions 40-200 ppm. The callus formation was then evaluated using high performance liquid chromatography methods to obtain qualitative and quantitative data. The results showed that the yield of epicatechin was obtained to 11.75%, suggesting that the supplementing of phosphor inducers at cultivation of in vitro culture of C. sinensis L able to improve the production of epicatechin by in vitro culture technique.

Reproductive phenology of Bruguiera sexangula (Lour.) Poir. in Berbak and Sembilang National Park, South Sumatra Sarno Sarno; Rujito Agus Suwignyo; Zulkifli Dahlan; Munandar Munandar; Moh Rasyid Ridho
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 2 (2018): June 2018
Publisher : The East Java Biological Society

Phenology is the study of the period phases which occured naturally in plants. Information about phenology is essential for the sustainable management of mangrove area. During these phases was significantly influenced by the circumstances surrounding environment as ever shines, temperature, and humidity. Observations of the phenology is devoted on mangrove Bruguierasexangula, especially since the phenology information about B. sexangula is still restricted in Berbak and Sembilang National Park, South Sumatra. Research was carried out in February-September 2016. The methods used in this research is a tagging method or labelling on flower buds and observed till the stage of being a mature propagule. Initiation of flowering B. sexangula was occurring in February, the next step was the flowering that occurs from April to June, while the bear season occured from late June-July. The stage of maturation of propagule until October. Harvest time of propagule B. sexangula was conducted in October. Flower initiation of B. sexangula stage to propagule maturity stage takes about 7 months.

The distribution and composition of Odonata (Dragonfly and Damselfly) in Sriwijaya University, Inderalaya Campus South Sumatera Yulia Pujiastuti; Yuanita Windusari; Muhammad Agus
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 1 (2017): December 2017
Publisher : The East Java Biological Society

The information about distribution and composition of Odonata in Sriwijaya University campus area is still not much. The decrease areas supporting the growth of Odonata influence distribution and composition of these species. The objective of research was to analyze the distribution and composition of Odonata in Sriwijaya University, Inderalaya Campus. This research had been conducted from December 2016 until February 2017 and continued in April 2017 by using visual observation, direct capture, and sticky traps. The sampling locations were determined by five points of observation station by using purposive sampling method. The result revealed Odonata living in Sriwijaya University, Inderalaya Campus is was aggregated and consisted of 22 species belonged to five families. The highest composition of species was found in Libellulidae (77.65%) followed by Coenagrionidae (17.86%).

The effects of cigarette smoke nanoparticles in the colorectal carcinogenesis of wistar rats Dody Novrial; Ignatius Riwanto; Sutiman B Sumitro; Indra Wijaya
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 1 (2017): December 2017
Publisher : The East Java Biological Society

Smoking cigarette is one of risk factors for developing colorectal carcinoma. Various researches have been made to suppress the toxicity of cigarette smoke. One of such effort is to reduce the size of cigarette smoke particles using nano water solution containing aromatic groups and EDTA. This study aims to assess the effects of this cigarette smoke nanoparticle regarding colorectal carcinogenesis. The experimental study was carried out with sequential post test only control group design. Thirty Wistar rats were divided into 3 groups: exposure to cigarettes smoke, exposure to smoke of cigarettes containing nano water solution and control for 14 weeks and 28 weeks. Colorectal epithelial morphology was assessed on the histopathology examination, whereas the expression of APC, KRAS, MSH2, MLH1, and p53 was assessed on immunohistochemistry procedure. Our results showed that cigarette smoke nanoparticles had better effects regarding colorectal epithelial morphology, especially through the increased expression of APC.

The traditional hunting on Greater Green Leafbird (Chloropsis sonnerati) in East Java: A vulnerable situation for vulnerable bird Agung S. Kurnianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 1 (2017): December 2017
Publisher : The East Java Biological Society

Greater Green Leafbird (GGL) Chloropsis sonnerati is one of the favorite songbird in Indonesia and live harmoniously among local people. However the increasing human needs creates a vulnerable situation, both to socio-economic and bird sustainability. This study aims to reveal the GGL tradi-tional hunting method in two villages within the national park. In addition, this study also aims to reveal the trading plot from the initial supply to the local traders and the fluctuation of GGL population in their natural habitat. We conducted two approaches: an explorative interview on bird traders in Malang, Jember, and Banyuwangi, and a deep interview on the national park rangers, society, and bird hunters. Local people generally use two methods for GGL hunting. Pikat method, was used to catch specific birds that mostly live on trees. The other method, net method, was not widely used by the traditional hunter. Population of GGL was considered as abundant in the past, when the demand was low and the hunting activity only conducted seasonally using pulot method. The high of demand, modernization of method, and regeneration of hunters shifted the sustainable harvest into exploitation. During 2001-2003, the trend of hunting began to decline the GGL population in nature.

An evaluation on cropping system of rice and corn in dry land during dry season Zainal Arifin; Amik Krismawati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Dry land has wide and diverse agroecosystem, which potential as growth source to produce dietary crops (foodstuff). The objective of the research was to study the appropriate managerial technique of cropping system for rice and corn to increase production and income of the farm operation in dry land during dry season. The research was conducted on dry land during dry season (DS) I in 2018 at Jatisari Village, Arjasa Subdistrict, Situbondo Regency, on the area of 10,000 m2 width. The research used Randomized Block Design with 4 treatments and 6 replications, includes : (a) Upland rice (20 cm x 20 cm), (b) Upland rice largo super (40 cm x 20 cm x 10 cm), (c) Upland rice largo super (40 cm x 20 cm x 10 cm) + corn (160 cm x 40 cm), and (d) Corn (75 cm x 20 cm). Results of the research showed that the intercropping system between upland rice largo super and corn produced higher yield, 8,151 kg/ha DGH and 2,092 kg/ha grains of corn. Besides that, yield of corn trimming was 2,446 kg/ha fresh biomass. The increase production of intercropping upland rice largo super with corn has increase income of the farmers, IDR. 41,923,100 with R/C ratio 3.25, so that it is economically feasible.

In vitro embryogenic callus induction of Carica pubescens Lenne and K.Koch using 2,4-D (2,4-Dichlorophenoxy Acetic Acid) and BAP (6-Benzylaminopurin) Shinta Shinta; Eko Budi Minarno; I’anatur Rofiqoh
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Carica pubescens Lenne & K. Koch has benefits as a producer of medicinal compounds. Propagation of C. pubescens is important to be done through in vitro embryogenic callus culture. This research aimed to determine the effect of 2,4-D growth regulators, 2,4-D and BAP interactions on the induction of embryogenic callus C. pubescens Lenne & K. Koch. This research is experimental, using a completely randomized design (CRD) with 25 treatments and 5 replications. Embryonic callus was induced by various concentration of 2,4 D (0 mg/L, 2.5 mg/L, 5 mg/L, 7.5 mg/L, and 1 mg/L) in combination with BAP (0 mg/L, 2.5 mg/L, 5 mg/L, 7.5 mg/L, and 1 mg/L). Data were analyzed using two-way ANOVA, followed by the Duncan Multiple Range Test (DMRT) (p>0.05). The result showed that the addition of 2,4 D and BAP as a growth regulator gave an effect on embryonic callus. Concentration of 7.5 mg/L 2,4 D and 0.5 mg/L BAP in callus 19 days after planting showed the most effective interaction of both inductors. The combination produced callus fresh weight at 1.09 g and 99% in percentage of explant callus area. Morphological and anatomical observations showed the characteristic of embryogenic callus as indicated by yellowish, textured callus friable, having a large core and containing starch grains also plastids.

Page 3 of 51 | Total Record : 507

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Sutiman Bambang Sumitro

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berkalahayati@yahoo.com

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wulidanisa@berkalahayati.org

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Jalan Surakarta No. 5 Malang, Indonesia

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Sutiman Bambang Sumitro

Contact Email berkalahayati@yahoo.com

Phone +62341570631

Journal Mail Official wulidanisa@berkalahayati.org

Editorial Address Jalan Surakarta No. 5 Malang, Indonesia

Location Unknown, Unknown INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

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Abstract

Abstract

Abstract

Contact Name
Sutiman Bambang Sumitro

Contact Email
berkalahayati@yahoo.com

Phone
+62341570631

Journal Mail Official
wulidanisa@berkalahayati.org

Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia

Location
Unknown,

Unknown

INDONESIA