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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 507 Documents
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KEANEKARAGAMAN, PERSEBARAN, DAN POTENSI JENIS-JENIS Garcinia DI INDONESIA Tahan Uji
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/340

Abstract

Indonesia has a high species richness of Garcinia (Garcinia spp.) and it is as a important basic materials for breeding of mangosteen (Garcinia mangostana). Based on examination of herbarium collections and literature study, there are 64 species of Garcinia encountered in Indonesia. Twenty-five of which occur in Kalimantan, 22 species in Sumatera and Sulawesi respectively, 17 species in Moluccas and Papua respectively, 8 species in Java, and 5 species in Lesser Sunda Island. Six species of those are as cultivation plants (Garcinia atroviridis, G. beccari, G. dulcis, G. mangostana, G. nigrolineata and G. parviflora), 58 species as the wild plants, 22 species as edible fruits, and 21 species as timber plants. Some members of Garcinia, e.i. G. malaccensis, G. picrorhiza and G. beccari are reported as rootstock potential species to be wedge-grafting by mangosteen (G. mangostana) seedlings.
PRODUKTIVITAS BIOMASSA DAN LAJU PERTUMBUHAN RUMPUT LAUT Eucheuma cottonii DI PERAIRAN WAEL SERAM BAGIAN BARAT PROVINSI MALUKU Johanis Fritzgal Rehena
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/341

Abstract

Biomass productivity and growth rate of Eucheuma cottonii has been done during three cultivation periods of the year 2008 in water territory Wael, in the west of Seram regency. Research of sea grass was conducted by float raft method (floiting method) systems monoline. Statistical design used Factorial Pattern Completely Randomized Design 3 × 3 × 2. Cultivation period as factor A consisted of A1 (period I), A2 (period II), and A3 (period III). The result of the analysis showed that cultivation period (factor A) and also initial weight (factor B) gave a real response to growth rate Eucheuma cottonii, and so do second interaction of factor (AB) gave real response. Average of biomass productivity during three cultivation periods of each different initial number weighing ranged from 108.65–664.76 gram/sea grass with daily growth number ranged from 2.15–6.86% / day
PENGARUH Saccharomyces cerevisiae TERHADAP KADAR LOVASTATIN DALAM ANGKAK YANG DIHASILKAN DARI FERMENTASI BERAS OLEH Monascus purpureus JMBA Evi Triana; Novik Nurhidayat
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/342

Abstract

Fermented rice by Monascus purpureus, namely angkak, produces lovastatin. Lovastatin has been believed and studied as antihypercholesterolemic agent. The level of lovastatin in natural occurred product is very low, result in high price in the market. Something which could increase lovastatin amount is required to be investigated. Therefore a study about the effect of Saccharomyces cerevisiae in stimulating the growth of M. purpureus and the best time for adding S. cerevisiae in order to yield optimal amount of lovastatin has been conducted. The result showed that the best time for addition S. cerevisiae yieldied optimal amount of lovastatin is the day 12.
POTENSI FORMULASI BAKTERI PEREDUKSI NITRAT WADUK SUTAMI MALANG DALAM MENGHAMBAT PERTUMBUHAN Microcystis C. Retnaningdyah; U. Marwati; Suharjono Suharjono; N. Ajijah; Marjono Marjono; A. Soegianto; B. Irawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/343

Abstract

The problem facing in water ecosystem espescially reservoir in Indonesia is the blooming of Microcystis. The specific objective of this research was to determined the ability of indigenous bacteria formulation from Sutami reservoir, which be known have a capability of reducing nitrate, to controlled Microcystis growth. The research methodology as follow: the bacteria formulation was isolated from Sutami reservoir that had been tested having potency to reduce nitrate, than we carried out to examine the effect of that bacteria formulation toward the growth rate of Microcystis. This experiment have been done in laboratory. The formula of bacteria and Microcystis were grown together in sterilized natural media from Sutami reservoir adding with NO3-N 5 ppm. The treatment of this research were aeration and non aeration and variation of light intensity (2–3 Klux and 5–9 Klux). Incubation was done in laboratory with constant light for 12 hours per day. The abundance of Microcystis and bacteria were counted every day for 13 days. Then, the environment parameters such as pH, conductivity, concentration of nitrate, nitrite and ammonium were measured every weeks. The experiment was done with three replications at the same time using completely randomized design. The research result showed that there were found 22 species of bacteria capable to reduce nitrate in selective media, but only six species have highest potency to reduce nitrate more than 90%. The consortium of the six bacteria with abundance 4 × 107 cell/mL able to controlled the growth of Microcystis in laboratory 80–95% be started at sixth day after incubation. The bacteria consortium was found in this research can be recommended to used as bioagent active to controlled Microcystis blooming in the water if culturing of these bacteria utilize medium having no potency as source of pollutant in the water.
TRANSFORMASI GEN SUCROSE PHOSPHATE SYNTHASE (SoSPS1) MENGGUNAKAN Agrobacterium tumefaciens UNTUK MENINGKATKAN SINTESIS SUKROSA PADA TANAMAN TEBU (Saccharum officinarum L.) Miswar; Bambang Sugiharto; Joedoro Soedarsono; Sukarti Moeljapawiro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/344

Abstract

Sucrose phosphate synthase (SPS; EC 2.3.1.14) plays an important role in partition of assimilated carbon in most plants. SPS catalyses the penultimate reaction in the pathway of sucrose synthesis, in which sucrose-6-phosphate (Suc6P) is synthesized from UDPglucose (UDPG) and fructose-6-P (Fru6P). To increase the capacity of sugarcane in sucrose synthesis, spindle leaves of sugarcane cv R579 were transformed with cDNA SoSPS1 from sugarcane under the control of constitutive promoter (35S CaMV) that constructed in pBI 121 (pKYS) using Agrobacterium tumefaciens. Based on PCR analysis, we have detected the existence of SPS transgene in some lines of transformed sugarcane, called line 4, 5, 6, and 7. The SPS transgene in transformed sugarcane could be expressed into translation level and increased the amount of leaves SPS protein, so the activity of leaves SPS was higher than wild type sugarcane as control. The transformed sugarcane line 4, 5, 6, and 7 showed 1.4–2.9 fold increases in SPS activity and 1,76–2,2 fold increases in leaves sucrose content. Increasing in SPS activity in transgenic sugarcane was coupled by the increase in invertase activity and ratio between sucrose and starch content.
Skrining dan Karakterisasi Parsial Senyawa Antifungi dari Actinomycetes Asal Limbah Padat Sagu Terdekomposisi Alimuddin Ali
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/345

Abstract

Actinomycetes are one of the most attractive sources of antibiotics and other biologically active substances of high commercial value. Actinomycetes have proved to be capable of biosynthesizing secondary metabolites bearing conspicuous structural diversity, which could be further enlarged by structure modification. The objective of this work was to isolate and characterize of actinomycetes were responsibility to biosynthesis of a compound as antifungal agent and parsial identifying of these substances based on TLCbioautography. The study was done by some steps activities i.e: Isolation of actinomycete, antifungal assay, extraction and examination of MIC and TLC-bioautography using fungal test.The result of these research revealed that a genera Streptomyces spp has majority of actinomycetes was obtained from sources of microbia (decompozing sagoo solid waste). Isolate AS0827 has two a different spots which as antifungal activity based on Rf values. MIC of antifungal subtances was obtained from the actinomycetes about 200 µg/mL.The genera was shown highest of inhibition zone from of all the genera was named isolate AS0827.
PROPORSI MIKROSPORA UNINUKLEAT PADA EMPAT KLON TEBU (Saccharum spp.) Suaib; Woerjono Mangoendidjojo; PDN Mirzawan; Ari Indrianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/346

Abstract

An experiment to study the two different morphological characters of four clones of sugarcane (Saccharum spp.) panicles containing more than 50% of uninucleate microspore development was conducted in Tissue Culture Laboratory at Biology Faculty, Gadjah Mada University, Yogyakarta, since March until May 2006. Morphological characters of both kinds of panicles i.e. unsheated- and sheated flowers from sheat flag leaf were observed. Mean, percentage, and standard deviation from the mean value of the six different stages of microspore development e.g. tetrad, early- and late-uninucleate, early- and late-binucleate, and multinucleate or pollen grains were statistically used in this calculation. All data percentages were analyzed by variance analysis through General Linier Model Procedure, and comparisons between means of the uninucleate microspore development based on the two different morphological characters of four clones was calculated by Least Significance Difference method. Comparisons between the two different panicles characteristics in accordance with the proportion of the uninucleate microspore development, however, were analyzed by T-student procedure. All calculations were done by using SAS program of computer statistics package. Result of the research showed that: (1) the unsheated panicles were contained less than 50% of uninucleate (early- and late-uninucleate) microspore development; (2) the sheated panicles tend to be in high proportion of early- and late-uninucleate microspore development, and multinucleate or pollen grains, and (3) the more away of spikelets or anthers positioned in the panicle or sub-panicle, the more number or percentage of uninucleate microspores development were tend to be gradually decreased.
EFEK EKSTRAK AKAR GINSENG JAWA DAN KOREA TERHADAP LIBIDO MENCIT JANTAN PADA PRAKONDISI TESTOSTERON RENDAH Dwi Winarni
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/347

Abstract

This research was designed to compare the potency and duration effect of Java ginseng and Korean ginseng root extract administration on sexual behavior of male mice. It was done experimentally on male mice (strain BALB, aged 8–10 weeks, weighed 25–35 g). Thirty eight mice were grouped to 4 (four) groups: First group was treated with solvent (as positive control), 2nd group was treated ethynilestradiol (EE2) (as negative control), 3rd group was treated with Java ginseng root extract, and 4th group was treated with Korean ginseng root extract. All groups were administered with EE2 0.56 mg/20 g bw/day for 9 days as pretreatment to decrease the testosteron level. After pretreatment, each group divided to 3 subgroups (each would receive treatment for 9, 18, and 27 days). Ethynilestradiol 0.56 mg/20 g bw/day was administered along treatment to keep testosterone level low, except to positive control group. Java ginseng and Korean ginseng root extract (equal with 1.4 mg ginseng root powder/ 20 g bw/day) and EE2 were administered orally. The level of testosterone after pretreatment was measured by RIA (radioimmuoassay) and changes in libido were determined by libido test. After the last treatment, 1 male mouse kept singly in individual cage. Libido test was carried out for 20 minutes. All of these activities during test recorded by handycam. The mice were observed for time from the introduction of female into the cage of male upto the first mount (mounting latency/ ML) and for the number of mounts (mounting frequency/MF). The results indicated that at low testosterone level, Java ginseng root extract administration at the dose equal with 1.4 mg ginseng root powder/20 g bw/day shortened mounting latency and increased mounting frequency but Korean ginseng extract root at the same dose gave negative effects. Duration of administration of both Java and Korean ginseng root extract didn’t give effect on libido.
KEMAMPUAN JAMUR TIRAM (Pleurotus ostreatus) SEBAGAI SUPLEMEN UNTUK PENINGKATAN SEKRESI AIR SUSU DAN DIAMETER ALVEOLUS KELENJAR AMBING MENCIT I.B. Rai Pidada; Listijani Suhargo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/348

Abstract

This study was aimed to know the effects of oyster mushroom supplement to milk secretion and mammary alveolus diameter of mice. The research was carried out under laboratory condition at the laboratory of Reproduction Biology, Faculty of Mathematic and Sciences, Airlangga University by using Complete Random Method. The study was designed by using totally 32 mice post partum (PP) and devided into four groups of treatment which consisted 8 female mice post partum of each group. The groups are: (a) the control groups, the groups that were given 0.2 ml aquadest, (b) the treatments of oyster mushroom solution with 3 variation of mushroom concentration, there are 2%, 4% and 6%. The oyster mushroom solution was given by gavage. The treatments were started on 3th to 12th day of lactation period. The increasing of milk secretion were showed by the increasing of body weight of offsprings. The data were collected from the difference result of the balance of weight body of offspring and the data were observed on 4th, 6th, 8th, 10th, and 12th of lactation periode and then on 13th day, the mice (five mice for each group) were killed to make histological preparat of mammary glands. The datas were analyzed by ANOVA and LSD test (a = 0.05). The results of this research showed that oyster mushroom supplement can increase milk secretion and mammary alveolus diameter of mice. Oyster mushroom with 6% concentration gave the best effects And there was a positive corelation between the weight of oyster mushroom that was used with the increasing of the oyster mushroom effects.
BIOAKTIVITAS INSULIN LIKE GROWTH FACTOR–I COMPLEX PLASMA SEMINALIS KAMBING TERHADAP KUALITAS SPERMATOZOA HASIL SENTRIFUGASI Suherni Susilowati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/349

Abstract

Series of exploratived laboratory experiment have been conducted to analysed the bioactifity of Insulin Like Growth Factor – I (IGF-I) Complex protein of goat seminal plasm on the membran plasm and acrosom cap of centrifugation sperm. First experiment was conducted to identificatify and isolate IGF –I Complex of goat seminal plasm by using Native - Polyacrylamide gel electroforesis. The result of analysis of Native – PAGE indicated that IGF-I Complex have molecular weight of 150 kDa. Second experiment was conducted to role of IGF-I Complex protein on the membrane plasm and acrosom cap of centrifugation sperm. Semen was collected by using artificial vagina from eight mature goat. Immediately after initial evaluation, semen was centrifugated with Bracket and Oliphant’s (BO) for 5 minutes 1800 rpm. And then divided two groups. Each group was supplemented with 3 × 106 of sperm concentration and then into group I was supplemented with BO medium and group two was supplemented with IGF-I Complex protein. The result of this experiment indicated that percentages of intact plasm membrane and intact acrosom cap were significantly (p < 0.05), between BO medium and IGF-I protein. In conclusion, IGF- I Complex protein is the optimal in improving plasm membran and acrosom cap of centrifugation sperm

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