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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 507 Documents
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HUBUNGAN KEKERABATAN BURUNG GELATIK JAWA (Padda oryzivora) DI PULAU JAWA BERDASARKAN KARAKTER MORFOLOGI R. Susanti; Margareta R.; Nugroho E.K.; Lidia Martanti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/360

Abstract

The study was conducted to evaluated the phylogenetics of the morphology character of gelatik jawa (Padda oryzivora). Fifty four P. oryzivora from natural population in Surabaya, Malang, Ngawi, Bekasi, Karawang, Cirebon, Bogor, Semarang, Magelang, Yogyakarta and Prambanan is adapted to condition of the research for 2 week. After individual identification card with bird ring and measured of the quantitative and qualitative of morphology characters, morphology characters of the bird were analysed of the phylogenetics. The phylogenetics of P. oryzivora population suggested that the bird from the geographic regions not a grouping. The high degree of migration is due to stress environment, especially of the broken of the forest, hunting, destroying and pesticides application. The migration of the population is due to the traffic of the trade from the region to the other region. This problem press to put the constitution into effect the trade embargo this species. The degree of the individual variation due to the degree of the migration, random breeding and selection. The degree of the inbreeding of the birds is not reported, so need to follow-up studies.
UJI DEGRADASI SELULOSA DARI JAMUR TANAH HUTAN BEKAS TERBAKAR WANARISET-SEMBOJA, KALIMANTAN TIMUR Suciatmih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/361

Abstract

In order to know the effect of isolation method on the occurrence and capability of soil fungi to degrade cellulose, a study was conducted in an over-burned forest in Wanariset-Semboja, East Kalimantan. Soil fungi were isolated using three isolation methods: incubation at 45° C, treatment with 50% ethanol for 15 minutes, and heat treatment at 70° C for 15 minutes. Plates for heat incubation and for other methods were incubated at 45° C and 27° C for three days, respectively. Cellulose degradation test of isolated fungi was examined using Carboxy Methyl Cellulose (CMC) media. Results showed that isolation method affected diversity and population of soil fungi. Heat treatment at 70° C for 15 minutes appeared to have highest diversity and population of soil fungi. Eupenicillium javanicum var javanicum (van Veyma) Stolk & Scott, Talaromyces byssoclamydoides Stolk & Samson, T. flavus (Klocker) Stolk & Samson, T. stipitatus C.R. Benjamin, and Penicillium argillaceum Stolk et al. were dominant in an over-burned forest in Wanariset-Semboja, East Kalimantan. Twenty-one isolated fungi degraded cellulose.
PERFORMANSI SAPI BALI PADA TIGA DAERAH DI PROVINSI BALI A. Supriyantono; Luqman Hakim; Suyadi; Ismudiono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/362

Abstract

The aim of the research was to know qualitative and quantitative traits of Bali cattle at three different areas geographically (lowland, midland and highland) at Bali province whether there are deviations or not. Variation in coat colour and some description about phenotypic Bali cattle are the main aspect for qualitative trait whereas body weight, some statistical vital (height at hip, body lenght, heart girth) and reproduction traits (mating system, origin of sire, condition of physiology, service per conception, calving interval and days open) of Bali cattle are the main aspect for quantitative trait. Sample of Bali cattle was taken in Tabanan (midland), Pulukan (lowland) and Karang Asem (highland) area about 2% of the total population. Each trait was measured at different age group. The age for each cattle was based on permanent incicy (PI) wich divided into 5 PI (PI0, PI1, PI2, PI3, PI4). The qualitative traits were found by direct observation at the field together with measurement of quantitative traits, body weight and some statistical vital. The result showed that in general Bali cattle are sexually dimorphic spesies, with bull being dark chestnut brown and cow and juveniles reddish brown. Both sexes have white rump patches and leggings. Both sexes carry horns, although they are much heavier and larger in the males.Quantitatively, bali cattle in Pulukan are much smaller than any other area followed in lack reproduction traits.
STUDI PEMANFAATAN KULIT KERANG SEBAGAI BAHAN PENYUSUN PADA PEMBUATAN LEM KACA M. Nadjib M.
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/363

Abstract

Shellfish are ones of sea’s product which use as food, and shell part as a waste. This experiment was done to utilize shell as main product in glass adhesive together whit Arabic gum and white part of chicken egg as sticky substances. The shell contended about 76,64% of CaCO3 which could repair adhesive working to increase shear strength and curing time. Adhesive were made by variation composition in gram number of shell powder and Arabic gum. The best composition was obtain on 68.45% of shell; 8.22% of Arabic gum; 1.42% of white part of chicken egg and 21.90% of water. This composition had 16,620.105 NM-2 of shear strength; 23.14% of volatility and 90.9583 pa.det or 909.5830 poise viscosity as optimal result at eleven days of curing time. In environment resistant test, shear strength decreased about 64.33% in acid; 17.96% in water; and 1.83% in base. Storage life test of adhesive was performed in closed box at temperature 27° C and fungi grew it in fourth week.
EKSPRESI GEN PENYANDI b-XILOSIDASE DALAM SISTEM pHIS1525/ Bacillus megaterium MS941 Sri Sumarsih; Ni Nyoman Tri Puspaningsih; Sofijan Hadi; Ami Soewandi J.S.
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/364

Abstract

The aim of this research was to express the β-xylosidase gene in the pHIS1525/ Bacillus megaterium MS941 system. The xyl gene was amplified from pTP510 and cloned into pHIS1525 in E. coli DH10β. The recombinant plasmid was transformed into B. megaterium MS941 by protoplast transformation. Transformants were selected by growing the recombinant B. megaterium MS941 on solid LB medium containing tetracycline (10 μg/ ml). The expression of β-xylosidase was assayed using 0.2% methylumbelliferyl-β-D-xyloside (MUX) and the proteins were analyzed by SDS-PAGE method. The β-xilosidase activity was determined toward p-nitrophenyl-β-Dxylopyranoside (pNPX) as a substrate and p-nitrofenol releasing was measured by UV/Vis spectrophotometer at λ = 405 nm. This research showed that recombinant B. megaterium MS941 expressed the β-xylosidase gene (xyl) and secreted it into the culture medium. The SDS-PAGE analysis of extracellular protein (culture medium) showed a 60,0 kD protein band. The recombinant Bacillus megaterium MS941 expressed and secreted the β-xilosidase into culture medium 5 hours after adding 5% xylose. The b-xylosidase activity was 0.441 unit/ml toward pNPX as a substrate.
GANGGUAN PEMBENTUKAN ATAP BUMBUNG NEURAL EMBRIO MENCIT AKIBAT INDUKSI 2-ME YANG BERTEPATAN DENGAN MASA NEURULASI PRIMER Eko Prihiyantoro; Win Darmanto; Samekto Wibowo; Mammed Sagi; Sri Kadarsih Soedjono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/365

Abstract

The objective of this study was to determine time of neural fold fusion at dorsal mid line neural axis after treatment 2-methoxyethanol (2-ME) to pregnant mouse during neurulation period and to observe relation process of point of neural fold fusion. Mice at 08:05 gestational days treatment with 2-ME dose 7.5 mmol/kg bw on the other hand control group injected with aqua bidest. Pregnant mice at 08:12, 09:00, 09:12 was sacrificed by dislocation cervix. Embryo was collected after observe with dissecting microscope for external morphology and fixation in Bouin solution and buffer formalin for histological preparation and immunohistochemistry process. Result showed that there was failure of first point neural fold fusion at junction of perspective fore brain and mid brain. The failure of neural fold fusion was caused by increasing apoptosis neuroepithelium. There were no relation process between first point of neural fold fusion and second point or another point of fusion. Failure of first point fusion not cause failure another point of fusion. Observation at 09:12 gestational days showed that the only first point fusion was still open but formation of another part neural tube have finished.
PEMANFAATAN EKSTRAK JAMUR Coriolus versicolor UNTUK MENINGKAT KAN JUMLAH TOTA L LEUKOSIT DAN MAKROFA G PADA TIKUS PUTIH WISTAR SETELAH PEMAPARAN 2-METHOXYETHANOL Sri Puji Astuti Wahyuningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/366

Abstract

The aim of this research was to increase sum of total rat’s leucocytes and macrophages using Coriolus versicolor exposure 2-Methoxythanol (2-ME). Coriolus versicolor extract have been primary active compounds are polysaccharide krestin (PSK). This compound PSK contains of ß-glucan. The immune cells had b-glucan receptor. From this reason, Coriolus versicolor extract could increase of immune system after exposed 2-ME. This research used 30 of 3–4 month female rats, and they were divided into 5 groups. K (control), P1 (exposed by 2-ME), P2 (administered with Coriolus versicolor extract before exposed by 2-ME), P3 (administered with Coriolus versicolor extract after exposed by 2-ME), P4 (administered with Coriolus versicolor extract before and after exposed by 2-ME). Coriolus versicolor extract used dose of 300 mg/kg body weight was given with gavage. 2-ME dose of 11 mmol/kg body weight was given with injection at cavum peritoneum. Number on leucocytes and macrophages counted by using Haemocytometer Improved Neubauer. The data was analyzed with ANOVA, further more if any differences were analyzed with LSD. Result of this study showed that (1) P1 decreased on the total number of leucocytes if compared with K, but increased on the total number of macrophage. (2) P2, P3, and P4 increased the total number of leucocytes and macrophage. Suggestion that can be proposed from this research was Coriolus versicolor extract can repair and increase immune system.
PENENTUAN KADAR STPP FOOD GRADE UNTUK MENINGKATKAN MASA SIMPAN IKAN NILA TILAPIA (Oreochromis niloticus L.) Leny Yuanita
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/367

Abstract

The aim of the study was to determine Sodium Tripolyphosphate Food Grade (STPP FG) concentration as soaking solution at the safe limit to increase the quality and storage period of Nila tilapia (Oreochromis niloticus L). The first stage was to determine the combination of STPP FG concentration and the length time of soaking; the independent variables are STPP FG concentration, soaking time; the dependent variables are: P2O5 content, organoleptic parameter. The second stage was to determine the storage period; the independent variables are STPP FG concentration, soaking time, and storage period; while the dependent variables are chemical and microbiological quality. Data analysis were qualitative and quantitative descriptive. The results of the first stage study revealed that the variation of the STPP FG concentration and soaking time to meet the prerequirement of P2O5 content and the quality of Nila tilapia were: 90 g/l – 1 hour soaking time, 60 g/l – 2 hours soaking time, and 60 g/l – 1 hour soaking time. The second stage study showed that the storage period of Nila tilapia was 6 hours when variations of 90 g/l STPP FG concentration and 1 hours soaking time were applied; while variation of 60 g/l STPP FG - 2 hours soaking time and 60 g/l STPP FG - 1 hours soaking time were 4 and 2 hours storage period respectively.
PEMBUKTIAN HORIZONTAL TRANSFER OF RESISTANCE GENES MELALUI UJI SENSITIVITAS ANTIBIOTIKA PADA BAKTERI GENUS Staphylococcus DARI KASUS BOVINE MASTITIS Mustofa Helmi Effendi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/368

Abstract

The experiment was carried out to show the evidence of horizontal transfer of resistance genes by antibiotics sensitivity test on bacteria of genus of Staphylococcus from bovine mastitic cases. The first step of the experiment was to prepare pure culture of Staphylococcus aureus and Coagulase Negative Staphylococci. Milk samples were collected from mastitic cases at the afternoon milking time. Preparation of pure culture of isolates were confirmed by MS agar, hemolytic activity, catalase and coagulase test. Both isolates were tested by using antibiotics sensitivity test. The result showed that the both isolates of Staphylococcus aureus and Coagulase Negative Staphylococci from Surabaya dairy herd were resistant against erythromycin antibiotic. Based on the result, it can be concluded that there was horizontal transfer of resistance genes between both isolates from same udder of dairy cows.
INDUKSI 2-METHOXYETHANOL PADA MASA PRENATAL SEBAGAI PENYEBAB KELAINAN OTAK PADA MENCIT Win Darmanto; Eko Prihiyantoro; Ria Harmonis
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/370

Abstract

2-Methoxyethanol (2-ME) and glycol esters are widely used as a solvents in the industry and plasticizers, and have becomes ubiquitous environmental pollutants. This study were designed to evaluate the malformation of brain development as an impact of 2-ME. Four groups of pregnant mice were injected to 2-ME dose 10 mmol/kg body weight at 7, 13, 15, and 17 days gestation respectively. Control group were injected with sterile water. At 18 days of gestation samples in groups 7 and 13 days gestation were sacrificed. Brain were remove and weight, morfologically and histologically were examined. Samples were divided into five groups, i.e. control group, 7th, 13rd, 15th and 17th days of gestation groups. Samples in 7th and 15th days of gestation groups killed in 18th days of gestation to observed fetal brain defect and decreased of fetal brain weight but some samples to allow delivery of fetuses. Furthermore, the fetuses killed on 0, 5, 10, and 20 days after birth, to take their brain. The result of these study showed that there are decreasing total weight of fetal brains and there are cerebral malformation as impact of 2-ME exposure.

Page 31 of 51 | Total Record : 507

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