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Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 507 Documents
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STUDI PERTUMBUHAN DAN PERKEMBANGAN KONDROSIT EMBRIO AYAM DALAM KULTUR DENGAN ASAM BORAT Nur Ducha; Mammed Sagi; Istriyati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/350

Abstract

The teratogenisity mechanism of boric acid is less known. There are contradictory observation on the effect of boric acid on the fetus malformation. An observation reported that boric acid is bound to the riboflavin and this complex causes a riboflavin deficiency on fetus. However, the other observation suggested that the present of riboflavin and boric acid had no decresed malformation on fetus, but increased malformation. It is giving an idea that the one of teratogenisity mechanism of boric acid may the biological mechanism to the target cells. The goal of this study was to evaluate the effect of boric acid on the growth and development of cultured chick embryo chondrocytes. Chondrocytes were dispersed from sternal cartilage of 12-d chick embryos (Gallus gallus). Randomized completely design was used to arrange this treatment with five replicates for each treatment. The dosage of boric acid were 10, 20, 40, 60, 80, and 100 μg/ml. The cultures were observed on 1, 3, 5, 7, 9 day after boric acid treatment, including: the cell proliferation, cell adhesion, cell attachment and colony formation. The data were analyzed descriptively. Result of this experiment showed that on high dose (80 and 100 μg/ml) cell adhesion was not formed, therefore the colony was not formed, and formed vacuola in the cell. This study indicated that boric acid on the high dose can inhibit the cell proliferation, cell adhesion, cell attachment and colony formation.
UJI PRODUKSI BIOSURFAKTAN OLEH Pseudomonas sp. PADA SUBSTRAT YANG BERBEDA Fatimah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/351

Abstract

Biosurfactant, microbial metabolite whose properties like surfactant, was suggested to replace chemically synthesized surfactant for take in hand environtmental pollution by petroleum hydrocarbon. This work was done to examine potency of Pseudomonas sp. isolated from Tanjung Perak Harbor to produce biosurfactant. Also, to know the effect of different substrates (glucose + yeast extract, lubricating oil and hexadecane) toward biosurfactant production. Pseudomonas sp. grown in mineral synthetic water and biosurfactant production was measured on stationary phase. Biosurfactant production based on emulsification activity and surface tension reduction of supernatant (using Du Nouy tensiometer). Solar, lubricating oil, and hexadecane were used to examine emulsification activity. Results indicated that Pseudomonas sp. have a potency to produce biosurfactant. Surface tension of supernatant decreased up to 20 dyne/cm, when grown on hexadecane substrate. Hexadecane is the best growing substrate for biosurfactant production than others.
POTENSI ANTIBODI SPERMATOZOA TERHADAP SPERMATOGENESIS DAN FERTILISASI PADA TIKUS PUTIH Indah Norma Triana
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/352

Abstract

The experiment was conducted the potential of antiibody sperm to spermatogenesis and fertility of the male rat. Ten of rat testis have been used in this research, then crushed and added with 10 cc saline and then were filtrated and centrifuged 1800 rpm for ten minutes. Twenty four of the male rat divided in to four groups. Group I: injected with saline 0.1 cc/sc/day for 45 days; group II: injected with testis suspension 0.1 cc/sc/day for 15 days; group III: injected with testis suspension 0.1 cc/sc/days for 30 days; group IV: injected with testis suspension 0.1 cc /sc/days for 45 days. Then each of the male rat were mated with female, then the number of the total of pups were counted. Male rat were killed and testis were collected for preparation of histology and count of the spermatocyte and spermatid. The results show that the number of the spermatocyte and spermatid has significant different in the group and control (p < 0.05). The number of the pups significantly different in the group and control (p < 0.05). Implication of this research was injected Antibodisperm on male rat can decrease of the number of spermatocyte, spermatid and the number of the pups.
HIDROLISIS BEBERAPA JENIS XILAN DENGAN ENZIM XILANOLITIK TERMOFILIK REKOMBINAN Ni Nyoman Tri Puspaningsih; Hery Suwito; Sri Sumarsih; Ali Rohman; One Asmarani
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/353

Abstract

The aims of this research were to know the ability of recombinant xylanolytic enzyme from recombinant E. coli DH5a (pTP510) to hydrolyze several commercial xylan and analysis the reduction sugar product. Recombinant xylanolytic enzyme (exo-xylanase, b-xylosidase and a-L-arabinofuranosidase) could hydrolyzed several commercial xylan (oat-spelt xylan, birchwood, wheat, rye, and arabinan) with xylanolytic activities are: oat-spelt xylan (1.73 U/mL), birchwood (0.92 U/mL), wheat (6.52 U/mL), rye (4.94 U/mL), and arabinan (3.40 U/mL). Xylanolytic enzyme assay use specific substrate p-nitrophenyl-b-D-xylopyranoside (pNP-X) shown xylosidase activity 15.869 U/mL. Hydrolysis product was analyzed by HPLC. The results showed that xylose, arabinose, and xylo-oligosaccharide were produced from birchwood, wheat, rye, and arabinan hydrolysis, although xylose and arabinose were produced from hydrolysis of oat-spelt xylan.
PEMANFAATA N EKSTRAK DAUN WUNGU {Graptophyllum pictum (L.) Griff.} UNTUK PENURUNAN KADAR KOLESTEROL SERUM DARAH MENCIT BETINA YANG DIOVARIEKTOMI Listijani Suhargo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/354

Abstract

The purpose of this research was conducted to evaluate the effects of “daun wungu” extracts to decrease total cholesterol, LDL (Low Density Lipoprotein) and HDL (High Density Lipoprotein) cholesterol of ovariectomized mice. This research used 24 ovariectomized mice and 8 normal mice. The ovariectomized mice were grouped in 3 groups for K1 (the treatment with aquadest, 0.05 ml), K2 (the treatment with fish oil, 0.05 ml) and P (the treatment with “daun wungu” extracts, 0.5 mg in 0.05 ml fish oil). All treatments were done for 20 days. At the end of the treatments, the blood (0.5 ml) was taken from cardiac by tuberculin disposable syringe. And then with fotometry, serum total, LDL and HDL cholesterol were measured. The result of this research showed that daun wungu extracts (0.5 mg in 0.05 ml fish oil) could not decrease HDL cholesterol, but it could decrease total and LDL cholesterol of blood serum in ovariectomized mice.
KAPANG ENDOFIT DARI TAMAN NASIONAL GUNUNG HALIMUN SEBAGAI PENGHAMBAT PERTUMBUHAN MIKROBA PATOGEN Salmonella thypi DAN Candida albicans Ruth Melliawati; Puspita Suci Wulandari
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/355

Abstract

Endophytic fungi live inside plants without harming the host. The purpose of this research was to screen endophytic fungi which could inhibit Salmonella thypi and Candida albicans, as well as characterizing of endophytic fungi, and antimicrobial compound produced by endophytic fungi. Some methods were used on this research. Diffusion agar plate methode was used to screen endophytic fungi which could produce antimicrobial compound against Salmonella thypi and Candida albicans. Standard plate count was used to measure fungi growth. Antimicrobial compound produced by endophytic fungi was analized by TLC and HPLC technique, compared with standard antibiotic, chloramphenicol and nystatin. The result showed 5 kinds of endophytic fungi produced antimicrobial compounds against Salmonella thypi. The largest zone of inhibition was 115 mm2 shown by Hl.25F.112. Two kinds of endophytic fungi were able to inhibit Candida albicans with the largest inhibiting zone was 164 mm2 shown by Hl.108F.481. The morphology of Hl.108F.481 indicated that this fungus had vertical hypha with sporangium at the end of the hypha and Hl.25F.112 had partioned hypha and oval-shape ascus. TLC and HPLC analysis showed that water extract of Hl.25F.112 (1s), chloroform extract of Hl.25F.112 (1c) and chloroform extract of Hl.108F.481 (2c) contained anti-microbial compound with retention time (RT) closed to chloramphenicol and water phase of Hl.25F.112 (1s) closed to nystatin.
ISOLASI DAN SELEKSI MIKROBA AMILOLITIK DARI MAKANAN FERMENTASI/RAGI TAPAI GAMBUT DI KALIMANTAN SELATAN Elidar Naiola
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/356

Abstract

Fourteen isolates of microbe which produce amylase were isolated from various fermented foods/ragi tapai at South Kalimantan. Three out of them were produce the higher amylase activities compared to others which shown the clear zone areas after pouring with Iodium solution with relative activities > 3. From the studies on morphological and physiological characterization, it was indicated that one isolate was belong to yeast, it was namely Candida sp (ISO RTG). The amylase activity of Candida sp (ISO RTG) was studied in media containing soluble starch, and result showed that the maximum activity of amylase reach after 3 days, it was 1.85 × 102 U/ml (one unit activity is define as μmol of glucose produce per ml per minute). In medium content rice flour as a carbohydrate sources, the maximum activity was 1.91 × 102 U/ml. The optimum temperature for enzyme reaction was at 40–45° C while optimum pH was at pH 5.0–6.0 and the enzyme was relatively stable under such conditions.
DEKLOROFILASI EKSTRAK PROTEASE DARI TANAMAN BIDURI (Calotropis gigantea) DENGAN ABSORBAN CELITE Yuli Witono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/357

Abstract

Biduri’ plant is a wild bush in tropical countries which is one of potential protease source. However, protease extracted from leaf and stamp top of biduri is still green in color due to contain a protein bounding-chlorophyll. It would be problem if it is used for some food. The objective of this rearch was to study a dechloroplyllation technique in order to obtain protease with low chlorophyll content but high specific activity. The results showed that the best dechlorophyllation method of biduri protease could be eluted by celite absorbance. The first step elution was obtained 16 ml filtrate of biduri protease with low chlorophyll. Consist to the result above also decreased protein content, with loading capacity was 1.067 gram biduri/gram celite or 0.015 μg chlorophyll/gram celite. However in the second step elution, after biduri filtrate has been freezed for 12 hours was obtained the dechlorophylated biduri protease was higher in loading volume. Resulting in increased of loading capacity to be 2.13 gram biduri/gram celite or 0.004 μg chlorophyll/gram celite. The chlorophyll decreased to about < 44% of chlorophyll from the first step elution, even the specific activity increased 286% compared with the first step elution.
CUTICULAR COMPONENT ANALYSIS FOR DISCRIMINATION OF Aedes Aegypti (DIPTERA: CULICIDAE) FROM SEVEN LOCALITIES IN SOUTH KALIMANTAN Abdul Gafur
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/358

Abstract

Cuticular components of female Aedes aegypti from seven localities (populations) in South Kalimantan were compared. Mosquitoes from four populations of Banjarmasin, and one population each of Pelaihari, Barabai, and Kotabaru were sampled. Cuticular hydrocarbons were removed from adult female and were separated and quantified by gas chromatography. Stepwise discriminant analysis determined the degree of differences between populations. Using the concentration of the hydrocarbons in a linear discriminant function and the estimator obtained from cross validation, it was demostrated that specimens could be correctly allocated the population to which they belong with an average success rate of 80.6%. All (100%) specimens of populations outside Banjarmasin were correctly identified. There was reduced segregation of the four Banjarmasin populations, suggesting greater similarity in the hydrocarbons of Aedes aegypti from these populations. It was suggested that the greater similarity correlated with increased contact between populations in the region.
PERAN ANTIBODI KUNING TELUR (IgY) SEBAGAI OPSONIN UNTUK PENCEGAHAN SERANGAN MUTAN STREPTOCOCCUS SEROTIPE D (STREPTOCOCCUS SOBRINUS) Okti Nadia Poetri; Retno D. Soejoedono; Agustin Indrawati; I Wayan T. Wibawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/359

Abstract

The aim of this study was to explore the role of serotype d Mutan Streptococcus (Streptococcus sobrinus) spesific immunoglobulin Y (IgY-Ss) as opsonin against the same strain. The eggs were collected from Single Comb Brown Leghorn which has been immunized with Streptococcus sobrinus. Agar gel precipitation test was applied to detect IgY-Ss in serum and egg. Egg containing IgY-Ss was collected and extracted by PEG-Amonium sulphate and purified using fast protein liquid chromatography. The purity of IgY-Ss was determined by UV spectrophotometer. Molecular weight was established by SDS-PAGE (sodium dedocyl sulphate-poly acrilamide gel electrophoresis). Biological activities of IgY-Ss as opsonin was determined by phagocytosis assay. Phagositic activity of macrophages was not increased by preincubation of both S. sobrinus (107 CFU/ml) and 100 μg of IgY-S, however the phagositic capacity was increased from 1.6 bacterial cell/ macrophag to 5.17 bacterial cell/ macrophag. These finding suggest that IgY-Ss obtained from hens immunized with S. sobrinus provide an alternative to prevent S. sobrinus infection.

Page 30 of 51 | Total Record : 507

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