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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 507 Documents
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PENGUJIAN VALIDASI ANALISIS KADAR ANDROGRAFOLID SECARA KROMATOGRAFI CAIR KINERJA TINGGI (KCKT) DENGAN ELUASI GRADIEN TERHADAP EKSTRAK HERBA SAMBILOTO (ANDROGRAPHIS PANICULATA NESS) Toetik Aryani
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/463

Abstract

Andrographolide of Andrographis paniculata Nees have been isolated and determined Extraction was carried out by maceration with ethanol as solvent. The concentration of Andrographolide was determined by HPLC method. The eluation was carried by out gradiently using methanol: water as an eluent and UV spectrophotometer at maks 228 as detector. The result of HPLC analysis are selectivity >1.2–1.5; r was 0.9937; precision was KV < 10%; accuracy > 90%; DL was 0.075; QL was 0.50 and PW < F table. Andrographolide content of Andrographis paniculata Nees from Banyuwangi was 6.25%, Kediri was 14.69% and Surabaya was 6.83%
PERKEMBANGAN SEL SPERMATOGENIK MENCIT (Mus musculus L.) SETELAH PEMBERIAN EKSTRAK KULIT KAYU DURIAN (Durio zibethinus Murr.) Anni Nurliani; Rusmiati; Heri Budi Santoso
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/464

Abstract

It has been conducted a study of identify population of spermatogenic cells of mice after induction Durian (Durio zibethinus Murr.) bark extract. This research used completely randomized design with five treatments i.e. control without treatment; control with aquadest; 5% durian bark extract; 10%; and 20% with five repetitions for each treatment. Extract was given per oral in volume dose of 0,5 ml/ 25–30 g/BW per day during 36 days. The result of this study showed that durian bark extracts caused significantly decrease of population of spermatogenic cells in concentrate 5%, 10%, and 20%.
ANALISIS STATISTIK PENGUJI KECENDERUNGAN POPULASI DAN VARIASI TIPE HABITAT BUAYA Crocodylusporosus DI DAERAH PASANG SURUT TELUK ARGUNI IRIAN JAYA Hellen Kurniati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 2 (2000): June 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/465

Abstract

The population ecology of saltwater (Crocodylusporosus) in Arguni Bay, Irian Jaya was studied during 19990-1998 period. In this study, the habitas were divided into five types (1. Dominant vegetation Nypafructicansand primary forest; 2.Dominant vegetations N. fructicans; 4.Mangrove; 5. Lake with dominate vegetation N. fructicans and mangrove, along the lake bank was covered by Barringtonia spp, Ficusspp and Callophylum spp, whereas almost at lake offshore was covered by lotus floating plant). Result of GLM statistical analysis showed there were no significant (p>0.05) interaction between habitat type and survey time, neither on the habitat type and time of survey. The Duncan test on habitat showed a grouping in three categories (A, AB, and B) in which habitat 5 was at first ranking. However, there was no grouping on time of survey. From this study it was concluded that mangrove habitat was essential for C.porosus life and used as a nursery ground.
KARAKTERISASI FRAGMEN DNA GEN GLUKOAMILASE (GLU1) PRODUK PCR DENGAN ANALISIS RESTRIKSI Sofijan Hadi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/466

Abstract

Characterization used retriction enzyme on the 1784 bp (base pairs) DNA fragmen of glucoamylase gene (GLUI) of E. fibuligera ITB. R. cc. 64 has been done. The rectriction enzyme usage was Stu 1, Eco RI, Eco RV, Bam HI and Sau 3A. The purpose of this research were: First was to know recognition site of 1784 bp DNA fragmen of glucoamylase gene (GLUI) by the restriction enzyme above. The second was to know homologyst the glucoamylase gene (GLUI) E. fibuligera ITB. R. cc. 64 and the glucoamylase gene (GLUI) Saccharomycopsis fibuligera HUT 7212 (pSf GLUI). The result of amplification glucoamylase gene (GLUI) indicated that 1784 bp DNA fragmen on GLUI locus has succesfully isolated and gave the same size with the positive control pSf GLUI. Analysis of those DNA fragmen by StuI, Eco RV, Eco RI, Bam HI and Sau 3A indicated that 1784 bp of DNA fragmen from E. fibuligera ITB.R.cc.64. has the same result with 1784 bp of DNA fragmen from pSf GLUI. The result of the fragments after incubated by restriction enzymes are as follows: ± 997 bp and 787 bp by Eco RI, 1000 bp and 1780 bp by Bam H) and 850 bp and 760 bp by Sau 3A. Digestion using StuI and Eco RI was failed. To ensure that the DNA fragmen 1784 bp has characteristic as glucoamylase gene, it should be expressed into S. cerevisiae and/or should be determined the nucleotide sequence by DNA sequencing.
AKTIVITAS KONTRASEPSI DAN INTERSEPSI EKSTRAK METANOL DAUN BENALU Dendrophthoe petandra L PADA MENCIT G N Astika
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 2 (2000): June 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/467

Abstract

It had been reported that leaf of Dendrophthoe petandra contained a flavonol glycoside of which quercetion was its aglycone. In-vitro study in the activity of quercetin indicated that quercetin inhibited the activity of sperm hyaluronidase. So it meant quercetin inhibited sperm penetration into cumulus. Other study proved that quercetin inhibited the activity of cytochrome P-450 III A 4 enzyme which catalyzed the reversible hydroxylation of estradiol-17β into estrone and futher into estriol. The elevation of estradiol serum level disturbed gamete and zygote transport in oviduct causing premature expulsion of zygote into uterus. This research was designed to provide information about the anti-fertility activity of methanolic extract of leaf of Dendrophthoe petandra, focused on its contraceptive and interceptive activity. Virgin female Balc-C mice were used as the test animals. Each study had two group of mice which were treated with 150 mg/30 g and 200 mg/30 g extract respectively. Control group were treated with respective vehicle at 1 ml/30 g. statistical analysis of data (p<0.05) concluded that the extract had contraceptive and interceptive activity. According to the criterion given by Lee andChi (1985), this methanolic extract had a priority for further study.
NEW RECORDS AND TAXONOMIC REEXAMINATION OF THE GENUS KELERIA (COPEPODA, POECILOSTOMATOIDA, LICHOMOLGIDAE) FROM INDONESIAN WATERS Mulyadi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/468

Abstract

Taxonomy and distribution were studied on the species of the family Lichomolgidae recently collected from 4 sites in Indonesian waters. Three species were recorded, including Kelleria pectinata (A. Scott, 1909) has been recorded previously from Indonesian waters, and Kelleria australiensis Bayly, 1971 and Kelleria regalis Gurney, 1927 were new records for the area. The distribution of these species in Indonesian waters and other regions in the world is compiled and discussed.
PENGHILANGAN GEN Lacz DARI PLASMID pUKC 815 UNTUK MEMBENTUK VEKTOR EKSPRESI DI RAGI Saccharomyces cereviceae Ahmad Thontowi; Ni Nyoman T P; Mariatun Loegito
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 2 (2000): June 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/469

Abstract

The pUKC 815 plasmid was a shuttle vector. This plasmid had the genetic marker to the urasil (URA3) in yeast and ampicillin resistanced marked in E. coli. The pUKC 815 plasmid also had a promotor gene PGK. The PGK promotor was expected to inducting the expression of amylase gene. Expression of amylase gene in S. cereviceaecould be do by preparing the pUKC 815 plasmid as an expression vector through reducing the LacZ gene then it would be replace by the amylase gene. This research used several methods. These method were isolate the pUKC 815 plasmid, the digestion of the pUKC 815 DNAwith the BamHI restriction enzyme, electrolution, ligation and the transformation of 8.000 pb fragment pUKC 815 plasmid in to the cell of E. coli DH5a. the result shown that the expression vector in yeast (S. cereviceae) from the pUKC 815 plasmid with the 8.000 pb was formed.
AKTIVITAS ANTIBAKTERI NBENZOILAMOKSISILIN DAN N34DIKLOROBENZOILAMOKSISILIN TERHADAP Staphylococcus ATCC 25923 DAN Escherichia coli ATCC 35218 Harry Santosa; Bambang Soekardjo; Ami Soewandi J S
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 2 (2000): June 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/470

Abstract

N-Benzolamoxicillin and N-3,4-dichlorobenzolamoxicillin were synthesized from amoxicillin by acylation with benzoylchloride and 3,4-dichlorobenzoylchloride respectively. Structure characterization of the amoxicillin derivative compounds were done by UV, IR and 1H-NMR spectroscopy. Both derivative compound has antibacterial antivity against Staphylococcus aureus and Escherichia coli. The activity of N-3,4-dichlorobenzolamoxicillin is more active than N-Benzolamoxicillin.
TOKSISITAS Bacillus sphaericus H-5a5b (VCRC B42i) TERHADAP LARVA Culex quinquefasciatus Salamun; Rosmanida; Ni’matuzzahroh; R.A. Samsumaharto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 2 No 2 (1996): December 1996
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/471

Abstract

Bacillus sphaericus H-5a5b is an entomoathogenic agent which showing high potency for vector control, highly specific to insect target, and do not produce any adverse environmental impact. Such agent would be promising for vector control in Indonesia. The present studies is aimed to observing the toxicity status of Bacillus sphaericus H-5a5b (VCRC B42) against Culex quiquefascitus larvae. The larvae of Culex quiquefascitus were reared under the laboratory conditions. The powder of VCRC B42 were prepared by Vector Control Research Centre (VCRC), India. Bioassays on VCRC B42 agains larvae of Culex quiquefascitus were also carried out under the laboratory conditions. The value of LD50 and LT50 for VCRC B42 were decided using probit analysis. The toxicity status was compared with standart of VCRC India. The result of the bioassays showed that the VCRC B42 was moderately toxic agains larvae of the Culex quiquefascitus. The value of LD50 for VCRC B42 was 0.058 mg/l and LT50 about 27 hours.
CHARACTERIZATION OF ENVELOPE E PROTEIN OF DENGUE VIRUS INDNESIA ISOLATE Rantam F A; Soetjipto Soetjipto; Dachlan Y P
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 2 (2000): June 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/472

Abstract

Dengue virus is a human pathogen that has as an increasingly important public health threat. These virus have four serotypes (DEN-1, DEN-2, DEN-3, DEN-4). There is no specific treatment for any pathogenic dengue virus and there are few vaccines available. This study was carry out to E protein characterization from dengue virus infected C5/36 cells for vaccine candidate. We found that E protein induced antibody response. The data of the reactivity on the nitrocellulose membrane between E protein against polyclonal and monoclonal antibodies on western blot analysis, shown that the E protein whisc was once of dengue virus protein has immunogenic. The Indonesia isolate had no difference with the Phillipine isolate. The result suggested that all of the E protein dengue virus strain have peptides overlapping. Also promising for he production of immunogenic particulate proteins, especially DEN protein for the four strains.

Page 41 of 51 | Total Record : 507

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