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Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 507 Documents
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Pengaruh Diet Tinggi Serat Pangan terhadap Absorpsi Fe dan Efisiensi Regenerasi Hb Rattus norvegicus Leny Yuanita
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/452

Abstract

The aim of the study were to find: 1)The effect of high intakes of dietary fiber on iron absorption and haemoglobin regeneration efficiency percentages, through combination of pH 4 and 7 with length of boiling time of 0 (raw), 5, 15, and 25 minutes. 2)The role of iron binding to dietary fiber on haemoglobin regeneration efficiency. The experiment involved yard long beans and 110 of male Rattus norvegicus (Wistar strains) as dietary fiber sources and animal model respectively. The results revealed that: 1) High intakes of yard long bean fiber will decreased iron absorption and haemoglobin regeneration efficiency. pH, boiling time and its interaction affected on iron absorption and haemoglobin regeneration efficiency (p < 0,05). On dietary fiber treatment, the highest iron absorption and haemoglobin regeneration efficiency at raw- pH 4 were higher than standard feeding treatment; while the lowest at pH 7- boiling time 5 minutes treatment. The treatment’s results at raw-pH 7 and pH4- boiling time 25 minutes were identical with standard feeding treatment. 2) An increase and decrease of boiling time and pH respectively increased iron absorption and haemoglobin regeneration efficiency, due to a decrease of iron binding to dietary fiber. The same results also occurred with non boiling treatment (raw condition) due to the role of exhancer substances on iron non heme absorption.
ABNORMAL STRUKTUR HISTOLOGIS KORTEKS CEREBELLAR TIKUS DENGAN NORMAL FOLIASI AKIBAT IRADIASI SINAR X MASA POSTNATAL Win Darmanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/453

Abstract

Our previous experiment showed that prenatal exposure of rats to X-irradiation on gestation day 21st as the late gestation period causes heterotopic Purkinje cells and abnormal foliation of the cerebellum. However the present study observed the heterotopik of Purkinje cell layer following exposure of the rats to X-irradiation on postnatal day (P) 4, without abnormal foliation of the cerebellum. It also demonstrated the process of re-derangement of Purkinje cells. Rat pups were exposed to 2.5 Gy X-irradiation and the cerebellum was examined histologically and by immunohistochemistry to identify Purkinje cells. At 6 h after exposure, extensive cell death was observed in the external granular layer (EGL) of the cerebellum. By P7 (3 days after exposure), while Purkinje cells with well developed dendrites aligned underneath the EGL in the control cerebellum, Purkinje cells with shorter and abnormally oriented dendrites failed to align and re-deranged in the heterotopic location. On adult, the cerebellum of rats irradiated was observed almost normal folial developed. It is strong support previous study that the critical stage for cerebellar abnormal foliation caused X-irradiation is in the early postnatal period.
Identifikasi Simplisia yang Dijual sebagai Strychnos ligustrina Bl. di Pasar Tradisional Surabaya dengan Metode Random Amplified Polymorphic DNA (RAPD) Oeke Yunita; Angelica Kresnamurti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/454

Abstract

Authentication of Strychnos ligustrina Bl. had been performed at molecular level (DNA) with Random Amplified Polymorphic DNA (RAPD) method, based on the amplification of random DNA fragments by Polymerase Chain Reaction (PCR) with a single arbitrary primer. The aim of this research was obtaining similar banding patterns between DNA of plant Strychnos ligustrina Bl. and DNA of its lignum on local market. Strychnos ligustrina Bl. was determined by UPT Balai Konservasi Tumbuhan Kebun Raya Purwodadi and plants sold as Strychnos ligustrina Bl. were collected as lignum from traditional market at Wonokromo, Rungkut, Genteng, Benowo dan Pabean. DNA from these plants were extracted by modified Cetyltrimethylammonium bromide (CTAB) method and amplified by RAPD method. Amplification had been performed by primer OPO-4 had shown banding patterns on the gel electrophoresis which banding patterns were shown by Strychnos ligustrina Bl. and plants sold as Strychnos ligustrina Bl. on Benowo. Based on this early result, we assume that plants sold as Strychnos ligustrina Bl. on Benowo has closely genetic relationship with Strychnos ligustrina Bl.
ANTI-PROLAKTIN SEBAGAI PENGHAMBAT PROSES MOULTING Husni Anwar; Erma Safitri
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/455

Abstract

A experimental study had been done using anti-prolactin as moulting process inhibitor and toknow the affect of anti-prolactin on the time of egg laying recovery of laying bird. The objective of this study was to prove that, moulting phase can be stopped with pasif imunisation antiprolactin intra muscular. The stages of this study as follow: used anti-prolactin from the third stage of study with the dose of 50 mg/ml (P1), 100 mg/ml (P2), and 200 mg/ml (P3) and 0.5 ml PBS (as control). In this stage, moulting was ceased respectively in day 4.8 ± 1.033 (P1); 4.6 ± 0.843 (P2); 4.68 ± 0.516 (P3) and 61.9 ± 2.079 (control). Statistical test using one way Anova revealed significant difference (p < 0.01) between control and treatment groups, and BNT 5% test showed that the fastest moulting inhibition was found in P3 group (200 mg/ml), which was not significantly different (p < 0.05) with P1 (50 mg/ml) and P2 (100 mg/ml). The time of egg laying recovery was respectively at 9.3 ± 0.675 (PI); 7.4 ± 0.843 (P2); 3.3 ± 0.823 (P3) and 18.4 ± 1.174 (control). Statistical test using one way Anova test showed significant difference (p < 0.01) between control and treatment groups, and BNT 5% test indicated that the shortest time of egg laying recovery was at group P3 (200 mg/ml), which was significantly different (p < 0.05) from Pl (50 mg/ml); P2 (100 mg/ml); and control (PBS 0.5 ml). Conclusively, anti prolactin (50 mg/ml, 100 mg/ml and 200 mg/ml) to inhibit moulting process and to affect the time of egg laying recovery.
PERDAGANGAN BURUNG-BURUNG PARUH BENGKOK DI BALI [Parrot Trade in Bali] W Widodo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/456

Abstract

The survey was carried out to know some aspects of the bird trade in Bali on May 2002, especially to observe the diversity and abundance of species of Psittacidae Family along with their problems. The survey was done by direct observations and interview to the traders in the Sanglah and Satria Bird Markets, Denpasar-Bali. The total of 80 spescies of birds was traded in Bali and most of them i.e.: 75 species or 1577 individuals were found in the Satria bird market, but only 37 species or 393 individuals traded in the Sanglah bird market. Psittacidae family was found more abundantly, i.e.: 428 individiuals in 13 species of parrots. Those spesies of parrots were: Violet-necked Lory (Eos squamata), Blue-streaked Lory (E. reticulata), Red Lory (E. bornea), Rainbow Lorikeet (Trichoglossus haematodus), Chattering Lory (Lorius garrulus), Cockatail Australia (Nymphicus hollandicus), Moustached Parrakeet (Psittacula alexandri), Peach-faced Lovebird (Agapornis roseicollis), Fischer’s Lovebird (A. fischeri), Masked Lovebird (A. personata), Bali Hanging Parrot (Loriculus pusillus) and Budgerigar (Melopsittacus undulatus). The Rainbow Lorikeet was found more abundant than another species of parrots, but Bali or Javan Hanging Parrot was very rare. The most catching area of Psittacidae was about 45% from Moluccas Islands.
POLIETILENA GLIKOL (PEG) DALAM MEDIA IN VITRO MENYEBABKAN KONDISI CEKAMAN YANG MENGHAMBAT TUNAS KACANG TANAH (Arachis hypogaea L.) Enni Suwarsi Rahayu; Edi Guhardja; Satriyas Ilyas; Sudarsono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/457

Abstract

The objectives of this experiment were to determine response of epycotyl of nine peanut cultivars against polyethylene glycol (PEG 6000) induced stress under in vitro conditions, effective concentration of PEG to inhibit growth and development of epycotyl, evaluate tolerance of the cultivars against PEG stress, and evaluate changes in total proline content due to PEG stress. Growing epycotyls from peanut seeds (TDK) or from embryo axis (TTK) were planted on liquid MS-0 medium containing PEG 6000 (0%, 5%, 10%, 15%, and 20%). Growth, development, and the tissue damage score of the epycotyl were observed after six weeks. Total content of proline were analyzed for stressed and non stressed epycotyl to determine effect of PEG stress on proline accumulation. Results of the experiment indicated that addition of PEG 6000 in to MS-0 medium inhibited growth and development of peanut epycotyls and increased its total proline content. Addition of PEG 6000 might be used to simulate drought stress under in vitro condition. PEG at 15% concentration was effective for inhibiting growth and development of epycotyl explant. The response of peanut epycotyls against medium containing 15% PEG 6000 might be used as alternative methods for screening peanut tolerance against drought stress. The TDK and TTK might be used as explant, while increased in shoot length (TTK), in leaf number (TDK and TTK), in milted leaf number (TDK and TTK), in root number (TDK) and score of tissue damage (TDK) might be used as criteria for drought tolerance.
METODE PEMBUATAN ANTIPROLAKTIN PADA HEWAN COBA KAMBING LOKAL SEBAGAI PENGHAMBAT PROSES RONTOK BULU PADA AYAM ARAB PETELUR Erma Safitri
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/458

Abstract

Anti-prolactin has a specific activity against prolactin. It neutralizes prolactin action in circulation. The effect of such neutralization is the inhibition of feather fall off process, so that hens may be able to produce eggs again. Anti-prolactin can be produced by injecting prolactin isolate from blood serum of arabic hens in feather fall off-phase into local goat. Prolactin isolate was immunized to local goat to produce anti-prolactin. Six local goats were divided into 2 groups. The first group comprised 1 goat immunized with PBS, and the second one was immunized with prolactin isolate in CFA and subjected to booster with prolactin isolate in IFA twice. The formation of anti-prolactin and the highest titer was detected using indirect ELISA. The results of this study showed that (1) Anti-prolactin could be produced in goat from the prolactin isolate of feather fall off-phase arabic hens blood serum; (2). The first emergence of Antiprolactin was at the first bleeding after immunization of prolactin isolate in CFA and first booster in IFA. The highest titer was found at eleventh bleeding after the third booster with prolactin isolate in IFA.
PENGARUH SUHU LINGKUNGAN TROPIS TERHADAP PENENTUAN JENIS KELAMIN DROSOPHILA G. Wayan Seregeg
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/459

Abstract

Based on many research studies conducted it can be concluded that there are more than 250 theories of sex-determination. This means that there is not any single theory on sex-determination. This study aims at uncovering effects of the temperature of the tropical environment on sex-determination in Drosophila. The method undertaken was to collect and count the ratio of males to females, Drosophila directly caught in natural environment. The catching was conducted in three periods, ie.: April 2001, April 2002, and April 2004.The results were as follows: (1) The number of females caught at each periode was much more than males. The ratio of males to females was 1:2; (2) The ratios of males to females at three successive periodes were significantly different at p ≥ 5. The ratios vary due to the influence of several factor, outside the temperature of the environment, on the ratios of males to females in Drosophila.
VERIFIKASI cDNA T29 SEBAGAI KANDIDAT GEN PENGKODE PROTEIN Toxoplasma gondii DENGAN METODE SDS PAGE Ira Djajanegara; Wayan Artama; Retno Lestari; Sabar Pambudi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/460

Abstract

The process of cDNA construction from mRNA isolated from Toxoplasma gondii has been done. There were 7 candidates cDNA which one of them is called T29. Since Toxoplasma gondii is the cause of toxoplasmosis infection, cloning the gene encoding protein from this parasite provides an important tool for developing diagnostic kit for detection of toxoplasmosis. Digestion of the cDNA T29 with EcoRI which is the restriction site where the cDNA was inserted yielded a 1.862 bp fragment. The fragment was subcloned into E. coli expression vector pMal-p2x and transformed into E.coli strain TB1. Colonies of TB1 were grown on ampicillin plates and the recombinant plasmid was extracted using the standard procedure. The plasmid was digested using EcoRI and PstI, checked by PCR amplification using malE and M13/pUC primers. The recombinant plasmid was expressed in TB1 and the protein extracted was ran in SDS PAGE to observe the presence of the expressed protein. Based on the data from this experiment, there was no expression result of the expressed cDNA which was confirm by the PCR result. Therefore, it was concluded that cDNA T29 was not carrying the gene coding for protein from parasite Toxoplasma gondii.
ELUSIDASI STRUKTUR ISOLAT SKUALENA DARI IKAN HIU CUCUT BOTOL (Centrophorus sp) Aniek Setiya Budiatin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/462

Abstract

The main source of squalene was liver oil of shark. Producing of squalene this oil could be separated from other components inside. The research obtained that the value of squalene was 75.83 ± 1.14%. To discover the purification and safety to consume squalene which had been isolated from oil of the liver’s shark cucut botol ‘ was used a method which available in AOAC, therefore that could be done elucidation of the structure of isolate with any instruments such as KLT; FTIR; GC; GC-MS; and NMR. Structure elucidation of isolate compare with standard of squalene had result as follows: a. KLT: Rf isolate = 0.457; Rf standard = 0,457; b. FTIR = 2980– 2850 CH of methyl and methylene; 1668.58 & 1446.74 a double chain of carbon (C=C); 1381.16 & 835.25 = branch chain that all for isolate was the same with standard; GC tR = 9.065 minute of isolate and standard was 9.124 minutes; GC-MS the main fraction with mass 69 was one unit of isoprene (C5H9); the value of proton NMR (δ = ppm) were 1.601 & 1.674; 2.016; 5.150 & 5.155 for isolate; the standard were 1.599 & 1.670; 2.016; 5.155. The result of elucidation structure could be concluded that the isolate was the same with the squalene standard. Therefore, isolate could be able to consume safety as natural health food for all consumers because it had already pure and free from other compound such as cholesterol.

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