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Jurnal Natur Indonesia

JN
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Published by Universitas Riau

ISSN : 14109379 EISSN : 25030345 DOI : https://doi.org/10.31258/jnat

Core Subject : Science, Agriculture, Engineering,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

Jurnal Natur Indonesia published since 1998, is a science journal that presents articles on research results, thoughts and views from researchers and experts in the field of biosciences (basic science), including biology, physics, chemistry and mathematics. In the field of biosciences (basic science); Biology, Physics, Chemistry, Mathematics.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 306 Documents

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Preparasi DNA Spesies Colletotrichum sp. dan Spesifitas Sistem Fingerprinting RAPD Jamsari, Jamsari
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

DNA preparation from C. capsici dan C. gleosporides is important for PCR based analysis of anthracnose causingpathogens. On the other hand, RAPD as one of DNA-based fingerprinting is hindered by its instability and its nonspecifity problems. For that reason, converting RAPD fragments into other DNA based systems is an alternative toincrease its analysis reliability. The study showed that mycelia grown in 2-days liquid culture is appropriate materialfor DNA preparation especially when combined with Shagai-Maroof protocol as well as Promega Genomic DNAIsolation Kit. The study indicated also that some isolated RAPD-fragments showed its instability character. Thiswas proved by the occurrence of multi different length of fragments after re-amplification of some single RAPDspecific fragments.

Perubahan Komposisi Kimiawi Produk Yogurt dengan Penambahan Kalsium Karbonat pada Kultur Starter Campuran Safari, Agus; Ghina, Sarah Fahma; Djajasoepena, Sadiah; ', O. Suprijana; Indrawati, Ida; Rachman, Saadah D.; Kamara, Dian S.; Ishmayana, Safri
Jurnal Natur Indonesia Vol 17, No 1 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Mixed lactic acid bacteria culture is commonly used in yogurt production. In the present study, two lactic acid bacteria (Lactobacillus bulgaricus and Streptococcus thermophillus) was used as starter culture. Calcium carbonate was added to the starter culture to increase the quality of mixed starter culture of L. bulgaricus and S. thermophillus with ratio of 4:1. The present study was directed to investigate the chemical composition of mixed starter culture with and without calcium carbonat addition. Furthermore, the effect of each starter culture on yogurt product chemical composition was also examined. The pH, lactose, soluble protein and acid content was determined as chemical composition parameters. For starter culture without calcium carbonate addition, the yogurt has pH, lactose, soluble protein and acid content of 4.18–4.39, 4.18–4.39% w/v, 2.88–4.36% w/v and 0.82–0.99% w/v, respectively. While for starter culture with calcium carbonate addition, the yogurt product has pH, lactose, soluble protein and acid content of 4.26–4.37, 1.47–1.75% b/v, 3.42–4.95% w/v and 0.86–1.11% w/v, respectively. Addition of 0.05% w/v calcium carbonate to mixed starter culture gave effect on lactose consumption, where it still can convert lactose to lactic acid up to 45 days of storage. Furthermore, the yogurt product made with starter culture with calcium carbonate addition has higher soluble protein content compared to yogurt made with starter culture without calcium carbonate addition

Pengaruh Ion Kadmium(II) dan Nikel(II) pada Ekstraksi Ion Tembaga(II) dengan Ekstraktan 4-Benzoil -1-Fenil-3-Metil- 2- Pirazolin-5-On Menggunakan Emulsi Membran Cair Hamzah, Baharuddin; Jalaluddin, Noor; Wahab, Abdul Wahid; Upe, Ambo
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The effects of cadmium(II) and nickel(II) ions to copper(II) extraction using liquid membrane emulsion with 4-benzoyl-3-methyl-1-phenyl-2-pyrazolin-5-on (HPMBP) as an extractant was studied. The optimum condition forcopper(II) extraction were as follows: emulsification rate=2000 rpm, emulsification time=10 minutes, extractionrate=300 rpm, extraction time=15 minutes, concentration of mixed surfactant (span 80+span 20)=3%, volumeratio of membrane phase and internal phase=1:1, concentration of HPMBP=0.020 M, concentration of HCl=1M,volume ratio of emulsion and external phase=1:7. The result showed that the extraction of copper(II) by liquidmembrane emulsion with HPMBP as an extractant was selective to cadmium(II) and nickel(II) ions, relatively. Theresult also showed that in the extraction of 500 mg/l copper(II), the presence of 500 mg/l of nickel(II) was decreasingthe percentage of copper(II) extraction to be 83.73. While, the presence of 500 mg/l of cadmium(II) does notinfluence the percentage extraction of copper(II), relatively.

Pembuatan Biodiesel dari Minyak Kelapa melalui Reaksi Metanolisis Menggunakan Katalis CaCO3 yang dipijarkan Padil, Padil; Wahyuningsih, Slamet; Awaluddin, Amir
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Biodiesel is one of alternative fuels. Biodiesel can be made from coconut oil and is referred to as cocodiesel. Thisresearch studies the optimum condition of cocodiesel production by methanolysis reaction between coconut oiland methanol using heterogen catalyst of calcium carbonate (CaCO 3) is calcined for 1.5 hours at 900oC. In order toget optimum condition, several experimental parameters are applied such as catalyst concentration 1-3 %-wt andmolar ratio of methanol/coconut oil 4:1-12:1. The optimum condition obtained from this experimental as follow:catalyst concentration was 2%, molar ratio of methanol/coconut oil was 8:1 at 600C, produced the higgest conversionof cocodiesel 75.02%. The cocodiesel meet the requirement of Standar Nasional Indonesia (SNI) specifications.Based on data analysis, the product has a qualification as diesel fuel. SNI Biodisel is density (40 0C) 850-890Kg/m3,kinematic viscosity (40 0C) 2,3-6,0 mm2/s, cetane number min 51, iod number max 115 gr iod/100 gram,moisture content max 0,05 % volum, acid number max 0,8 mg KOH/g, flash point min 100 0C.

Keanekaragaman Spesies Parasitoid Telur Hama Lepidoptera dan Parasitisasinya pada Beberapa Tanaman di Kabupaten Solok, Sumatera Barat Hidrayani, Hidrayani; Rusli, R.; Lubis, Y. S.
Jurnal Natur Indonesia Vol 15, No 1 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

A study was conducted to determine the species diversity of lepidoptera egg parasitoids on several crops in Solok. lepidopteran eggs were collected from rice, corn, tomato, and cabbage. A survey was conducted by using Stratified Purposive Sampling Method. Two districts (Gunung Talang and Lembah Gumanti) were chosen as centre of the target crops. The results showed that five species of egg parasitoids were found parasitizing lepidopteran eggs. The species found on rice were Telenomus sp., Tetrastichus sp., and Trichogramma japonicum parasitizing Scirpophaga incertulas and Hesperiidae with parasitization levels were 29.54; 13.02; and 2.05% respectively. The species found on corn was Trichogrammatoidea spp. parasitizing Helicoverpa armigera with parasitization level was 29.07%. None of parasitoid was found to parasitize eggs of H. armigera on tomato. The egg parasitoid parasitizing Crocidolomia pavonana and Spodoptera litura on cabbage was Trichogramma chilotrae with parasitization level was 1.67%. The most abundance species of lepidopteran egg parasitoids were Telenomus sp.and Trichogrammatoidea sp. The diversity index of egg parasitoids on rice in different sample areas varied from 0.46 to 0.63 based on Simpsons index. It indicated that the species diversity of lepidopteran eggs was relatively low.

Sintesis Nanopartikel Nd-CeO2 Menggunakan Metode Simple Heating untuk Aplikasi Solid Oxide Fuel (SOFCs) Sriyanti, Ida; Abdullah, Mikrajuddin
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Nd-CeO2 nanoparticles have been synthesized with a simple heating method. The microstructure and morphologyof material were characterized by a Scanning Electron Microscopy (SEM), and the conductivity of the material wasmeasured by an AC Impedance Spectroscopy. At room temperature we obtain a sample prepared at 9% at/at. Ndhave the highest conductivity of 1.47 x 10-4. Scm-1. This conductivity surpass the value reported by Anefleous for Nd-CeO2 (NDC) material prepared by different method. Sample has been 49 nm crystals size obtained from FWHM .Thisresult proves that a simple heating method is the promising for synthesizing electrolyte materials for SOFCapplication.

Enzyme–Catalysed Synthesis of Palm-Based Wax Esters-A Kinetic Study Gunawan, Erin Ryantin; Basri, Mahiran; Suhendra, Dedy
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Wax esters are long chain esters that are derived from fatty acids and alcohols with chain lengths of 12 carbons or more. Wax esters havea wide range of application in industrial especially in cosmetics. The present work focuses on the synthesis of wax esters by alcoholysisreaction from palm oil and oleyl alcohol in hexane by lipase from Rhizomucor meihei (Lipozyme IM). Effect of various concentrations ofpalm oil and oleyl alcohol were studied to deduce the kinetics and mechanism of the reaction. The alcoholysis reaction followed Michaelis-Menten kinetics. The reaction follows a Ping-pong Bi-Bi mechanism. The maximum rate was estimated to be 6 x 10 -3 mmol/h. mg catalystand the Michaelis-Menten constant for palm oil and oleyl alcohol were 4.145 M and 6.120 M, respectively.

Isolasi dan Karakterisasi Protease Ekstraseluler dari Bakteri dalam Limbah Cair Tahu Fatoni, Amin; Zusfahair, Zusfahair; Lestari, Puji
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Protease has been used in large application industrial process such as detergent, leather, textil, softdrink, andmedicine. In order to find unique protease, many substances were explored as proteases of bacteria sources. Inthis study, tofu liquid waste was used as a source of bacteria producing proteases. Waste sample was growth inskim milk agar medium showing proteases activity, it was used to produce extracellular protease. The microbialcolonies were identified as Staphyllococcus sp. Protease was extracted with 5000 g centrifugation at 4 0C, andpurificated with ammonium sulphate precipitation continued with dialisis. Optimum production time, pH, metal ion,EDTA, specific activity, KM, and Vmaks were studied for enzyme characterization. Volume of crude enzyme was 300ml, with spesific activity of 3.55 U/mg. Protease obtained from 60% ammonium sulphate fraction had the highestspecific activity of 68.22 U/mg. Study on the protease characterization revealed that optimum temperature of thisenzyme was 400C. The optimum pH of the enzyme was found to be 8.0. The kinetic parameters K M dan Vmaks withcasein as substrate were 0.31% and 51.55 U/ml. Some inhibitory effect was observed in the presence of EDTA, Cu +2,Co+2, Zn+2, and enzyme activity was stimulated by Mg+2, indicating that this ion had a functional role in the molecularstructure of the enzyme.

Kajian Kondisi Terumbu Karang dan Strategi Pengelolaannya di Pulau Panjang, Air Bangis, Kabupaten Pasaman Barat Oktarina, Angreini; Kamal, Eni; Suparno, Suparno
Jurnal Natur Indonesia Vol 16, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Coral reef ecosystems are essential for the survival of marine life, but the presence of coral reef are declined and decayed.Methods used in this study was a survey method. To retrieve the condition of coral cover using the line intercept transectwhile to determine management strategies using SWOT analysis. The average percentage of coral cover in 8 point researchstations at 50.10% are still in the good condition, which is in the north 3 m depth 53.51%, north 7 m depth 68.14%, east 3 mdepth 93.29%, east 7 m depth 15.93%, south 3 m depth 75.17%, south 7 m depth 20.53%, west 3 m depth 41.50%, and west7 m depth 32.79%. The strategies for coral reef ecosystem management, among others: 1) community-based management, 2)forming Kelompok Masyarakat Pengawas (Pokmaswas) institutions, 3) establish in Panjang Island as a marine conservationareas, 4) Ko-Management-based, 5) effort to improve the economy in order to support the increased knowledge of theimportance of conservation and management of coral reef ecosystem.

Efek Sumber Karbon Berbeda terhadap Produksi â-Glukan oleh Saccharomyces Cerevisiae pada Fermentor Air Lift Kusmiati, Kusmiati; Thontowi, Ahmad; Nuswantara, Sukma
Jurnal Natur Indonesia Vol 13, No 2 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The need of â-glucan is increasing in food, medicine and cosmetic industry, because it becomes anticancer,antitumor and antiaging, increases immunosystem, and decreases cholesterol content in blood. The cell walls ofS. cerevisiae contain 80-90% polysaccharides that posses â-glucan. This research was aimed to obtain appropriatecarbon sources to increase the production of â-glucan. The carbon sources used were glucose, glucose commercial,sucrose and molases. The fermentation process was done by using air lift fermentor. The steps of fermentatonincluded regeneration of S. cerevisiae strain, preculture, fermentor preparation and running fermentor for 84hours. Sampling of S. cerevisiae culture was determined the cell growth by optical density (OD) usingspectrophotometer UV/VIS at ë 550 nm. The protein content was determined by Lowry method at ë 755 nm and thetotal glucose was measured by phenol sulphate method at ë 490 nm. The measurement result of cell growthshowed that the high intensity of S. cerevisiae in medium contain molases, but it did not show significant effectwhen compare to other carbon sources. The protein and carbohydrate contain in medium tended to decrease. Theresult of â-glucan on glucose, sucrose, glucose commercial and molases were 933,3, 1100, 1000, and966,7 mg/l. It can be concluded that sucrose and glucose commercial can replace the glucose to produce of â-glucan, because they are cheaper and easier to get. Beside that, molases can be used as an alternative carbonsource because it can produce of â-glucan as well as glucose.

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Home Page

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Editorial Team

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Contact Name Irwan Effendi

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Journal Mail Official kurniawanronal09@gmail.com

Editorial Address Kampus Bina Widya KM. 12,5 Simpang Baru, Tampan, Pekanbaru 28293 Indonesia

Location Kota pekanbaru, Riau INDONESIA

Abstract

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Contact Name
Irwan Effendi

Contact Email
natur@ejournal.unri.ac.id

Phone
+62811764031

Journal Mail Official
kurniawanronal09@gmail.com

Editorial Address
Kampus Bina Widya KM. 12,5 Simpang Baru, Tampan, Pekanbaru 28293 Indonesia

Location
Kota pekanbaru,

Riau

INDONESIA