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Jurnal Natur Indonesia

JN
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Published by Universitas Riau

ISSN : 14109379 EISSN : 25030345 DOI : https://doi.org/10.31258/jnat

Core Subject : Science, Agriculture, Engineering,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

Jurnal Natur Indonesia published since 1998, is a science journal that presents articles on research results, thoughts and views from researchers and experts in the field of biosciences (basic science), including biology, physics, chemistry and mathematics. In the field of biosciences (basic science); Biology, Physics, Chemistry, Mathematics.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 306 Documents

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Genetic Diversity and The Relationship Between The Indonesian Mangosteen (Garcinia Mangostana) and The Related Species Using Isozyme Markers Sinaga, Soaloon; Sobir, Sobir; Poerwanto, Roedhy; Aswidinnoor, Hajrial; Duryadi, Dedy
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Indonesia was known to have high diversity of mangosteens (Garcinia mangostana) and the related species. Inorder to elucidate the genetics variability of the diversity, thirty three accessions were examined by using isozymeanalysis. The genetic diversity and relationships among several mangosteens and other Garcinia sp wereestablished by using four isozymes. The level of polymorphism as revealed by isoenzyme was 88%. Althoughmangosteen is believed to reproduce exclusively through apomixis, our result show that considerable geneticdiversity exists within G. mangostana and between other Garcinia species. Based on 27 bands there were 5-42%dissimilarity level among mangosteen accessions, while the other species has 75% dissimilarity. The dendrogramis built based on isozyme marker analysis to separate clusters of mangosteen and other Garcinia sp. The datashowed that G. mangostana is a close relative of G. malaccensis, G. porrecta, G. celebica, and G. hombroniana. Theconcurrence analysis on isozyme analysis result showed the very good fit of Rolf correlation value (0.914). Thisresult indicated that isozymes could group G.mangostana and the related species.

Isolasi Gen Penyandi Peroksidase melalui Penapisan terhadap Pustaka Genom Kedelai Kultivar Slamet Suharsono, Suharsono; Rachman, Faisal; Astuti, Utut Widy
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Screening to genomic library of soybean cultivar Slamet in ë phage to isolate the gene encoding for peroxidase(per) was performed by two steps. cDNA of per gene from Arabidopsis thaliana labeled by alkalin phosphatasewas used as probe. The first screening was done by using 105 recombinant pfu (plaque forming unit). The secondscreening was carried out on 100 recombinant pfu isolated from the first screening. The result of second screeningshowed that all screened clones were supposed containing per gene. The ë phage resulted from second screeningwas infected into E. coli BM 25.8. Due to the Cre recombinase in E. coli BM 25.8, two lox P sites flanking the insertDNA of soybean created recombination. The recombination in loxP sites resulted the excision and formedrecombinant plasmid containing per gene of soybean. To multiply, the recombinant plasmid was introduced into E.coli DH5á. Analysis southern hibridization of recombinant plasmid cut by Bam HI showed that Bam HI 3.7 kbfragmen of S52121R clone, 5,3 kb of S2412R clone, 2,4 and 5,3 kb fragments of S2532R clone were supposed tocontain per genes.

Penapisan Aktivitas dan Senyawa Antidiabetes Ekstrak Air Daun Dandang Gendis (Clinacanthus nutans) Nurulita, Yuana; Dhanutirto, Haryanto; Soemardji, Andreanus A.
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

An investigation of the antidiabetic activity of Aqueous Extract and its fractions from a different polarity solvent:ethanol, ethyl acetate, and n-hexane of Dandang Gendis Leaves (Clinacanthus nutans Lindau) on Mile SwissWebster Mice has been carried out. The effect of aqueous extract of dose of 50, 100, and 150 mg/kg BW wastested on glucose serum level of alloxan induced that mice. Its fractions were tested by glucose tolerance method.Aqueous extract (150 mg/kg BW) significantly decreased blood glucose serum level (pd”0,05) that had the sameeffect as oral glibenclamide (0,65 mg/kg BW) administrated once a day for 9 days. Aqueous extract that wasfractionated has 5 fraction. Fraction of ethanol extract that was insoluble in ethyl acetate at a dose of 100 mg/kgBW was the most active fraction, that significantly (p<0,05) decreased blood glucose serum level of mice atglucose tolerance method of 18,4% in 3 hours after 2 g/kg BW of glucose administration. Thin layer chromatography(TLC) of that fraction ethanol 95%-chloroform 9:2 as eluent gave 5 spots under ultraviolet ray and showed spesificcompounds by qualitative test. Spot 1 (steroid) gave Rf 0,14; spot 2 and spot 3 that gave Rf 0,53 and 0, 67 wereflavonoid, spot 4 (triterpenoid) gave Rf 0,77; and spot 5 (chlorophyll) gave Rf 0,89. This research show that this plantcan used for hipoglicemic traditional medicine.

Synthesis and Antimalarial Activity of 2-Phenyl-1,10-Phenanthroline Derivative Compounds Hadanu, Ruslin; Mustofa, Mustofa; Nazudin, Nazudin
Jurnal Natur Indonesia Vol 15, No 1 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

To develop new potential antimalarial drugs of 2-phenyl-1,10-phenanthroline 5 derivatives from 8-aminoquinoline as startingmaterial were synthesized in good yields. The synthesis of 2-phenyl-1,10-phenanthroline 5 derivatives compoundswith 8-aminoquinoline 4 as starting material through three steps has been carried out. The first step of reactions is aldolcondensation of benzaldehyde 1 with acetaldehyde 2. The result of reactions is cinnamaldehyde 3 (92.14%) in the form ofyellow solid. The second step of reactions was synthesized of 2-phenyl-1,10-phenanthroline 5 (brown solid, 54.63%)through cyclization of 8-aminoquinoline 4 with cinnamaldehyde 3 compound. The third step of reactions is methylation andethylation of 2-phenyl-1,10-phenanthroline using dimethyl sulphate (DMS) and diethyl sulphate (DES) reagents that it wasrefluxed for 17 and 19 h, respectively. The results of reactions are (1)-N-methyl-9-phenyl-1,10-phenanthrolinium sulphate 6and (1)-N-ethyl-9-phenyl-1,10-phenanthrolinium sulphate 7 in yield from 90.62% and 89.70%, respectively. The results oftesting in vitro antiplasmodial activity at chloroquine-resistant Plasmodium falciparum FCR3 strain to 2-phenyl-1,10-phenanthroline 5 derivatives obtained that (1)-N-ethyl-9-phenyl-1,10-phenanthrolinium sulphate 7 compound has higherantimalarial activity (IC 50 :0.13 ± 0.02 μM) than antimalarial activity of (1)-N-methyl-9-phenyl-1,10-phenanthrolinium sulphate6 compound (IC 50 :0.25 ± 0.01 μM) and 2-phenyl-1,10-phenanthroline 5 compound (IC 50 :2.45 ± 0.09 μM). While, the resultsof testing in vitro antiplasmodial activity at chloroquine-resistant Plasmodium falciparum D10 strain to 2-phenyl-1,10-phenanthroline 5 derivatives obtained that (1)-N-methyl-9-phenyl-1,10-phenanthrolinium sulphate 6 compound has higherantimalarial activity (IC 50 :0.10± 0.04 μM) than antimalarial activity of (1)-N-ethyl-9-phenyl-1,10-phenanthrolinium sulphate7 (IC 50 :0.18 ± 0.01 μM) and 2-phenyl-1,10-phenanthroline 5 compound (IC 50 :0.55 ± 0.07 μM).

Produksi Asam Levulinat dari Inulin Umbi Dahlia (Dahlia sp. L) Menggunakan Katalis Asam Klorida Murwindra, Rosa; Linggawati, Amilia; Yanti, Pepi Helza; Awaluddin, Amir; ', Saryono
Jurnal Natur Indonesia Vol 16, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Dahlia is a floriculture plant with tuber that rich of inulin. Inulin is soluble in hot water and it will settle on cool temperature in alcohol. Extraction of result from 100 g dahlia tuber taken from Bukittinggi, West Sumatra is 4% inulin (w/w dahlia tuber). Inulin flour produced is white gray. Qualitative tests with Seliwanoff and Nelson-Somogyi methods shows that inulin positive contained of fructose and reducing sugar. Extracted result of inulin can be converted into levulinic acid using acid catalyst at high temperature. In this experiment, production of levulinic acid will bundle with variate temperature, reaction time and catalyst concentration of hydrochloride acid. The reaction of inulin convertion was done in ampul that burned in oven. Conversion result of 5% inulin to be levulinic acid on heating 150 o C hydrochloride acid concentration 0,1 M about 25 minutes was 12,33% (w/w inulin). On heating 170 o C hydrochloride acid concentration 0,1 M about 15 minutes is 26,25% (w/w inulin), while on heating 150 o C hydrochloride acid concentration 1 M about 15 minutes is 32,28% (w/w inulin). Experiment result showed that concentration of glucose, levulinic acid and formiat acid increase with increasing in reaction reaction temperature while fructose concentration decrease from 55,14 mg/mL to be 0,12 mg/mL. The longer time reaction and the higher concentration of catalyst hydrochloride acid, the lower concentration of glucose and fructose and the larger concentration of levulinic acid and formic acid.

Perbanyakan Tanaman Pulai Pandak (Rauwolfia serpentina L.) dengan Teknik Kultur Jaringan Yunita, Rossa; Endang, Endang; Lestarai, Gati
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Due to over exploitation of its bark for medicinal herbs and made worse by problem in conventional breeding, Rauwolfia serpentina (Pulaipandak), has been considered rare and was currently reported to be an endangered species. Therefore, conservation measure is urgent to betaken. One of them is by in vitro propagation. In this research, in vitro propagation covers several activities, such as (1) shoot inductionwith the application of MS (Murashige and skoog) media enriched with ZPT 0.0; 0.1; 0.3 mg/l BAP combined with 0, 1, 2 mg/l 2ip, (2)shoot multiplication by using 0.0; 0.5; 1.0 mg/l BAP combined with 0.0; 0.1; 0.2 and 0.3 mg/l thidiazuron), (3) root induction IBA at theconcentration of 0.0; 0.5; 1.0; 1.5; 2.0; 2.5; 3.0 mg/l, and (4) acclimatization. The result showed that the best shoot induction for calli isthe in vitro stem by the application of MS + 0.3 mg/l BAP + 1 mg/l 2ip basic media. For shoot multiplication, the best media was MS + 0.5mg/l BAP + 0.1 mg/l ; while the best formula for root induction was MS + 1 mg/l IBA. The best media for plantlet acclimatization is compost+ soil mixture in 1:1 ratio.

Seleksi, Karakterisasi, dan Identifikasi Bakteri Penghasil Kitinase yang Diisolasi dari Gunung Bromo Jawa Timur Soeka, Yati Sudaryati; Sulistiani, Sulistiani
Jurnal Natur Indonesia Vol 13, No 2 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Selection, characterization and identification of bacteria that can produce chitinase enzyme were isolated fromBromo Mountain, East Java. The 48 isolates were tested for capability to degrade chitine qualitatively, semiquantitatively and quantitatively. The result showed that 2 isolates, B2-4 and NA S4-1 could degrade chitin, withactivities of 4.8. 10-3 and 3.1 . 10-3 U/ml, after 1 and 2 days incubation respectively. By using molecular characterizationmethods, partial sequences of 16S rDNA and the primers 9F & 1510R were identified as Stenotrophomonas sp.

Makroalgae di Paparan Terumbu Karang Kepulauan Anambas Kadi, Achmad
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Macroalgae research in Anambas Islands that is the reef flats Jemaja, Pemutus and Matak Waters was carried onJune 2005. Survey method on the research using quadrat transect. Macroalgae is a marine flora which containsChlorophyceae, Phaeophyceae and Rhodophyceae. Biodiversity in the research location was 18 species. Thehigher diversity index (H) was 0.24 and equitability index (e) was 0,105. The higher density of species were 207individuals. Dominant species was 22,83% by Sargassum polycystum.

Studi Etnobotani Dioscorea spp. (Dioscoreaceae) dan Kearifan Budaya Lokal Masyarakat di Sekitar Hutan Wonosadi Gunung Kidul Yogyakarta Purnomo, Purnomo; Daryono, Budi Setiadi; Rugayah, Rugayah; Sumardi, Issirep
Jurnal Natur Indonesia Vol 14, No 3 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Dioscorea spp. were cultivated as alternative food in the garden and yard on dry season, and it’s planted by peoples in the villages. The objectives of the research are to identify the species, the tuber use, and the local wisdom to support the conservation of Dioscorea around Wonosadi forest. Samples and data collection was conducted in 15 villages around Wonosadi forest. Interview survey was done on peoples who are cultivate yam species (Dioscorea spp.) in their garden. Research were emphasized to tuber characteristic, traditional uses, and local wisdom to supporting yam conservation. Data was analyzed descriptively to illustrate the kind of local usage and local wisdom of connect with Dioscorea spp. The results showed that there were found species of gembili (D. esculenta (Lour.) Burk.), uwi (D. alata L.), and gadung (D. hispida dennst.) as cultivated plants, and tomboreso (D. pentaphylla L.), gembolo (D. bulbifera L.), and jebubuk (D. numularia Lamk.) as wild species in the forest. Tuber is raw material for many kind of food based on carbohydrate content. Gadung were used as fickle food with high diversification and insecticide for rice and corn. Peoples more consume gembili and uwi than gembolo and others species as rice substitute at dry season. The local wisdom were indicated that peoples still consume gembili and uwi as rice substitute, while tuber also used as sesaji in traditional concept jajan pasar or tukon pasar, and tuber of uwi was used as diet for diabetes complaint, and row material of uwi ungu tuber was used effectively for diarrhea complaint in the villages.

Fenologi Mangrove (Rhizophora apiculata, R. mucronata dan R.stylosa) di Pulau Unggas, Air Bangis Pasaman Barat, Sumatera Barat Kamal, Eni
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Phenology of tropical forest plant is a population factor and does not include community factor, this is caused by a genetic determinationand also the influence of the environment. Phenology of plants in mangrove correlated with the time of flowering, fruiting, and productionof fruits or propagules. Phenology research in mangrove plants in the Unggas Island, West Pasaman, done with ‘tagging’ technique method.Signs made of plastic and are numbered. Data collection included from the formation of flower blooming, up to the formation of fruit orpropagules ripe. Phase of the development is categorized to two categories: (a) flower, and (b) fruit. The analysis method is the analysis ofvariance (ANOVA, p<0.01) and post-hoc test, Duncans Multiple Range Test (DMRT, p<0.01) to obtain comparison and differencesbetween the components of flower, flower fall, fruit, and fruit maturity.

Page 12 of 31 | Total Record : 306

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Home Page

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Editorial Team

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Contact Name Irwan Effendi

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Journal Mail Official kurniawanronal09@gmail.com

Editorial Address Kampus Bina Widya KM. 12,5 Simpang Baru, Tampan, Pekanbaru 28293 Indonesia

Location Kota pekanbaru, Riau INDONESIA

Abstract

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Contact Name
Irwan Effendi

Contact Email
natur@ejournal.unri.ac.id

Phone
+62811764031

Journal Mail Official
kurniawanronal09@gmail.com

Editorial Address
Kampus Bina Widya KM. 12,5 Simpang Baru, Tampan, Pekanbaru 28293 Indonesia

Location
Kota pekanbaru,

Riau

INDONESIA