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Jurnal Natur Indonesia

JN
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Published by Universitas Riau

ISSN : 14109379 EISSN : 25030345 DOI : https://doi.org/10.31258/jnat

Core Subject : Science, Agriculture, Engineering,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

Jurnal Natur Indonesia published since 1998, is a science journal that presents articles on research results, thoughts and views from researchers and experts in the field of biosciences (basic science), including biology, physics, chemistry and mathematics. In the field of biosciences (basic science); Biology, Physics, Chemistry, Mathematics.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 306 Documents

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Medium Term Conservation of Several Carnation Accessions Via in Vitro Culture Budiarto, Kurniawan; Marwoto, Budi
Jurnal Natur Indonesia Vol 13, No 2 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Sufficient genetic diversity is important in carnation breeding program. In vivo conservation of carnation germplasmis considered inefficient due to some technical and economical aspects. In vitro conservation was then, expectedto overcome the limitation of in vivo method. The research was conducted to find out the proper media for medium-term in vitro conservation of several carnation accessions in low temperature storage. A complete factorialexperiment with 25 replications was designed to accomplish the combination of two factors. The first factor wassix commercial carnation cultivars, namely Pink Maladi, Orange Triumph, Opera, Tundra, Yellow Liberty and PradoReffit. The second factor was the conservation media i.e. 1⁄2MS + DMSO 3% and 1⁄2MS + 3% DMSO + 3% sucrose andcontrol (MS 0+3% sucrose). The results showed that in vitro conservation of carnation in low temperature weresuccessfully conducted using 1⁄2MS+3% DMSO and 1⁄2MS+3% DMSO+3% sucrose without significant variation in allaccessions tested up to 10 and 12 months respectively. The increase of death plantlets, however, was detected onthe media of 1⁄2MS+3% DMSO after 6 months storage with significant decrease in viability hereafter. The existenceof sucrose in DMSO media induced root formation and plantlet resistance to low temperature storage.

Construction of a DNA Vaccine Using Glycoprotein Gene and Its Expression Towards Increasing Survival Rate of KHV-Infected Common Carp (Cyprinus carpio) Nuryati, Sri; Alimuddin, Alimuddin; Sukenda, Sukenda; Soejoedono, Retno Damayanti; Santika, Ayi; Pasaribu, Fachriyan Hasmi; Sumantadinata, Komar
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Deoxyribonucleic acid (DNA) vaccine has recently been developed as an alternative vaccine against virus infection.This study was the first step of DNA vaccine development to protect cyprinids including common carp (Cyprinuscarpio) and fancy koi (Cyprinus carpio) from KHV (koi herpesvirus) infection in Indonesia. One of KHV glycoproteingenes, i.e. glycoprotein (GP) was ligated with Japanese medaka (Oryzias latipes) â-actin promoter to generatepAct/GP as a DNA vaccine. Fourty fish in body weight of 10-15 g/fish were individually injected by pAct/GP intomuscle in different dosage of 2.5 μg, 7.5 μg and 12.5 μg/100 μl phosphate buffer saline. Total RNA was extractedfrom the 12.5 μg of pAct/GP-injected fish muscle at 24, 48 and 67 hours post-injection to analyze GP expression byRT-PCR method. Potential of pAct/GP as DNA vaccine was examined by injecting KHV into the 30-days-vaccinatedfish. Both of possitive and negative control fish group were not vaccinated. Possitive control fish group wereinjected with KHV, but negative control fish group were not. KHV-challenged fish were reared for 1 month, and thedeath fish were calculated daily. Result of RT-PCR analysis showed that GP gene expression were detected at 3 dpost-injection. Expression of GP in the vaccinated fish groups helped to improve their survival rate after challengedby KHV. All of fish without DNA vaccination had dead 17 days after KHV injection. The results demonstrated thatpAct/GP had high potency to be used as a DNA vaccine against KHV infection in cyprinids.

Biomassa Kerang Anadara granosa pada Perairan Pantai Kabupaten Indragiri Hilir Nasution, Syafruddin
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The present study was carried out to investigate the biomass of cockle (Anadara granosa) from coastal waters ofSouth Indragiri District, Riau Province. The biomass was investigated by quadrad sampling method in the intertidalarea along the Concong beach, where fishing of cockles mainly occured. The first station was near by the Concongestuary, while the second station was about 3 miles away from the fisrt one. Samples were collected from threedifferent portions of the intertidal zone that of High Intertidal Mid-Intertidal, and Low Intertidal. The result showedthat the highest biomass was found at the lower partion of the intertidal zone. Soft tissue of A. granosa was about24.8% of total wet-weight and 7.2 % of dry-weight. While material organic was about 85 % of soft tissue dry-weight.Water qualities of the sampling station showed that the overall water qualities were still in normal condition.

Microbiological and Ecophysiological Characterization of Green Algae Dunaliella sp. for Improvement of Carotenoid Production ainuri, Muhammad Z; Kusumaningrum, Hermin P.; Kusdiyantini, Endang
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

An isolate of green algae Dunaliella sp. from BBAP Jepara is usually used as a source for carotenoid supplementfor marine animal cultivation in the local area. In order to improve carotenoid production especially detection ofbiosynthetic pathway from the organisms investigated in this study, the main purpose of this study is characterizingDunaliella sp. based on it’s microbiological and ecophysiological characters. The research was done by characterizethe growth, the cell and colonies microbiologically, total pigment production, and also characterize all of theecophysiological factors affecting the algal growth and survival. The results of this research showed that Dunaliellasp. posseses typical characteristic of green eucaryote alga, in their growth and ecological condition. The extremecharacters which was toleration ability to high salinity environment of was used to conclude Dunaliella sp. asDunaliella salina.

3,3’-di-O-methylellagic acid, an Antioxidant Phenolics Compound from Sonneratia alba Bark Herawati, Netti; Firdaus, Firdaus
Jurnal Natur Indonesia Vol 15, No 1 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Free radicals play an important role in some pathogenesis of serious diseases, such as neurodegenerative disorders, cancer,liver cirrhosis, cardiovascular diseases, atherosclerosis, cataracts, diabetes and inflammation. Compounds that can scavengefree radicals have great potential in ameliorating these diseases. It is reported that phenolic compounds in plants possessstrong antioxidant activity and may help to protect cells against the oxidative damage caused by free radicals. Previousstudy revealed that mangrove trees, Sonneratia alba Bark showed strong antioxidant activity. Ethyl acetate fractionexhibited the best antioxidant performance. The antioxidant activity of this fraction was attributed to the presence ofcompounds of different polarities such as phenolics. Furthermore, the phenolic antioxidant of ethyl acetate fraction werepurified and identified with UV, IR, MS, and 2D NMR spectrometry. 3,3’-di-O-methylellagic acid was found in brownamourphous powder. Antioxidant activity was evaluated and compared with L-(+)-ascorbic acid as standard by usingDPPH assay. The compound has strong antioxidant activity than ascorbic acid standar with the value of IC 50 11.35 and17.64 μg/mL, respectively. The high value of antioxidant activity of compound indicate that S. alba is a potential source ofnatural antioxidant agent.

Pengaruh Penambahan Sargassum sp. dan Inositol dalam Pakan terhadap Pertumbuhan dan Daya Tahan Juvenil Ikan Gurami (Osphronemus gouramy Lac.) Lestari, Dwi; Widiastuti, E. L.; Nurcahyani, N.; Susanto, G. N.
Jurnal Natur Indonesia Vol 16, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Gourami (Osphronemus gouramy Lac.) as one of the freshwater fishes, are easily to be cultured but slow in growth rate. One of the ways to enhance their growth rate is by feeding them with high nutritious food. Sargassum sp. and inositol are known compound able to stimulate the growth of some fishes. The study was conducted to determine the effect of Sargassum sp and inositol on commercial feed to the growth rate and survival of juvenile gouramy. The study was conducted from January to April 2015 in Aquatic Laboratory of Biology Department - University of Lampung. Completely randomized designed was used with 4 treatment groups and 5 replications. Anova and Tukey at 5% levels were applied to this study. The result indicated that addition of inositol and Sargassum sp on commercial food significantly affected the gourami growth parameters such as the body weight, body length, body width as well as juvenile gouramy spesific growth rate and their survival rate.

Isoterma dan Termodinamika Adsorpsi Kation Cu2+ Fasa Berair pada Lempung Cengar Terpilar Bahri, Syaiful; Muhdarina, Muhdarina; Nurhayati, Nurhayati; Andiyani, Fitri
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Pillared Cengar clay have been synthesized by two methods, first clay suspension is directly mixed into aqueous solution of hydroxy-aluminum polycations (WK) and second by mixing the clay suspension into the solution of sodium acetate and hydroxy-aluminumpolycations (SAK) sequentially. Both clays were calcined in air on atmospheric condition. Diffraction pola, surface morphology and cationexchange capacity of the pillared clays were characterized using X Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) andvisible spectrophotometry methods, respectively. The pillared clays showed increases of basal spacing from 3.57 Å to 4.55 Å and smectiteas a new mineral. Morphology of SAK has more heterogeneous surface with small plates and agglomeration of grains compare with WKwhich small plates. Adsorption of aqueous cation of Cu 2+ were studied on various variables of initial concentration as well as temperatures.As the result, adsorption of cation Cu 2+ on pillared Cengar clay is corresponding to Freundlich isotherm, while the adsorption capacity ofWK on cation Cu 2+ is slightly lower than SAK. The thermodynamic aspect, the WK is reflected possessed exothermic processes withnegative entropy, increased in Gibbs energy and non spontaneous, while the SAK possessed endothermic processes having positive entropy,decreased in Gibbs energy and non spontaneous.

Cyclodextrin Formation Initiated by Enzyme Debranching Reaction On Amylopectin Branch Chain Of Tapioka Laga, Amran
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Degradation of starch by the glucosyltransferase enzyme (CGTase) to produce the primary product of chainsplitting undergoes an intramolecular reaction without the participation of water molecule. From this process, α-1,4-Linked cyclic product, known as cyclodextrins, are formed. The aim of the research was to cut amylopectinbranch in order to produce one straight chain, to optimize cyclic reaction formation cyclodextrin by CGTase. Theresearch was devided into 3 stages; (1) debranching enzyme concentration estimation (5,10,15,20, and 25 unit/gram) and the length of otimum reaction to produce straight chain for 5 hours which sample was taken each hour,(2) reaction length time estimation to form cyclodextrin in order to use debranching products (straight chain) assubstrates, the reaction length for 360 minutes and sample taken each for 30 minutes, and (3) the best substrateconcentration for straight chain (20-40% w/v) to produce cyclodextrin. The result showed that enzyme concentrationtreatment and optimal length reaction will produce straight chain with enzyme concentration of 14 units/gram for3 hours and straight chain product of 20 units/gram for 1 hour with straight chain product of 83.5%. The optimumlength of reaction for cyclodextrin formation from amylose produced from the de-branching process was 240minutes. The amount of cyclodextrin produced was 143.45 g/L with conversion value of 47.81% at 30% (w/v)substrate concentration. Highest yield of cyclodextrin (154,28 g/L) and conversion value of 44.08% was obtained at35% (w/v) substrate concentration

Keragaman Genetik berbagai Isolat Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hyphomycetes) dan Virulensinya terhadap Crocidolomia pavonana Trizelia, Trizelia; Santoso, Teguh; Sosromarsono, Soemartono; Rauf, Aunu; Sudirman, Lisdar I
Jurnal Natur Indonesia Vol 14, No 3 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The objectives of the research were to study the genetic variability of various Beauveria bassiana (Bals.) Vuill. isolates andvirulence to Crocidolomia pavonana. Thirteen isolates of B. bassiana were isolated from Nilaparvata lugens (Stål),Leptocorisa oratorius (F.), Thrips sp., Hypothenemus hampei (Ferr.), Crocidolomia pavonana (F.), Spodoptera litura (F.),Chrysodeixis chalcites (Esp.), and Riptortus linearis (L.) pests from different geographical locations in Indonesia. Fourteendifferent random primers were used to amplify DNA. Clustering of isolates were obtained using Unweight Pair GroupMethod Avarage (UPGMA). The results showed that only OPA08 primer generated more polymorphic DNA fragments.Result of clustering analysis showed that B. bassiana isolates forms two groups having genetic similarity equal to 55%.Clustering of isolates does not correlate to host type nor geographic origin. Mortality of second instar C. pavonana larvaewas dependent on the fungal isolates. At a concentration of 108 conidia/ml, isolate Bb-La2 had the highest virulence whichcaused 65% mortality of 2nd instar larvae. Isolates of B. bassiana is virulent against C. pavonana larvae present in the samegenotype group.

Pewarisan Sifat Densitas Stomata dan Laju Kehilangan Air Daun (rate leaf water loss RWL) pada Kacang Tanah (Arachis hypogaea L.) Adisyahputra, Adisyahputra; Sudarsono, Sudarsono; Setiawan, Kukuh
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The aim of this research is to analyze and examine the inheritance of stomatal density trait and RWL as a variable in drought tolerance ofpeanut. The experiment was conducted by using cv. Kelinci that is sensitive genotype as female parent and US 605 which is tolerantgenotype as male parent, including population off spring from hybrid cv. Kelinci (P1) with US 605 (P2). Stomatal density was determinedby making leaf imprint and by observing leaf imprint under microscope. Relative water loss was determined by dipping peanut leaf in PEG40% for 48 hours. Result of the analysis showed that stomatal density and RWL were not only controlled by qualitative characters of majorgene, but also controlled by quantitative character of minor gene by polygenic with the complex gene action. Both characters seem toinfluence more as genetic factor and have high level fixation additive varians which can give the opportunity to obtain the tolerant offspring.

Page 16 of 31 | Total Record : 306

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Irwan Effendi

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Home Page

OAI Link

Editorial Team

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Reviewer

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Contact Name Irwan Effendi

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Phone +62811764031

Journal Mail Official kurniawanronal09@gmail.com

Editorial Address Kampus Bina Widya KM. 12,5 Simpang Baru, Tampan, Pekanbaru 28293 Indonesia

Location Kota pekanbaru, Riau INDONESIA

Abstract

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Contact Name
Irwan Effendi

Contact Email
natur@ejournal.unri.ac.id

Phone
+62811764031

Journal Mail Official
kurniawanronal09@gmail.com

Editorial Address
Kampus Bina Widya KM. 12,5 Simpang Baru, Tampan, Pekanbaru 28293 Indonesia

Location
Kota pekanbaru,

Riau

INDONESIA